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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 孫岩章(En-Jang Sun) | |
dc.contributor.advisor | 孫岩章(En-Jang Sun | eirl5622@ntu.edu.tw | ), | |
dc.contributor.author | I-Sun Lei | en |
dc.contributor.author | 李綺璇 | zh_TW |
dc.date.accessioned | 2023-03-19T21:09:11Z | - |
dc.date.copyright | 2022-09-14 | |
dc.date.issued | 2022 | |
dc.date.submitted | 2022-09-05 | |
dc.identifier.citation | 1. 郭克忠、許秀惠。2003。葡萄銹病。植物保護圖鑑系列11-葡萄保護。植物保護圖鑑系列。行政院農業委員會動植物防疫檢疫局。250頁。101-103頁。 2. 行政院農業委員會林業試驗所。2020。樹木褐根病診斷及防治建議手冊。行政院農業委員會林業試驗所。 3. 安寶貞、蔡志濃、張東柱、王姻婷。2005。台灣果樹及木本觀賞植物立枯型真菌性病害之調查與分布。植物病理學會刊 14 (3):203-210。 4. 李碧峰。2013。花木修剪基礎全書。麥浩斯出版。 5. 吳孟玲、許育晏、李芷芸、吳厚德、洪挺軒。2014。微生物製劑在褐根病防治管理之應用研究。台灣林業科學 29:41-53。 6. 吳孟玲、陳昭翰、李芷芸、劉則言、王瀛生、洪挺軒。2011 。木材的腐朽菌PCR快速檢測技術建立。中華林學季刊 44(4):501-516。 7. 吳美麗。2001。台灣兩種盤菌目真菌。植物科學期刊。46(3):238-245. 8. 李豐在。2005。草莓灰黴病之防治策略。花蓮區農業專訊 54:12-13。 9. 呂凱珮。2016。常見樹木腐朽病菌的鑑定及防治之研究。國立台灣大學植物病理與微生物學系碩士論文。 10. 邱志明、林振榮、唐盛林、湯適謙、羅卓振南。2004。不同藥劑塗布對櫸木修枝傷口變色與腐朽之效應。台灣林業科學 19(3):177-186。 11. 周文能、張東柱。2005。野菇圖鑑。遠流出版社。440頁。 12. 林正忠、蔡淑芬。2005。番石榴瘡痂病。植物保護圖鑑系列15-番石榴保護。防檢局。台北。205頁。 13. 孫岩章。2015。台大樹木褐根病預防性防治第一期試驗計畫。國立台灣大學植物醫學研究中心。 14. 孫岩章。2016。樹木褐根病預防性及治療性藥劑注射防治成果。植栽及樹木之醫療及健檢研討會論文集。臺灣植物及樹木醫學學會。 15. 孫岩章。2018a。植物醫師指南。五南圖書出版公司。 16. 孫岩章。2018b。樹木褐根病及靈芝根基腐病之預防及治療性藥劑注射方法及成果報告。2018植栽及樹木之健檢與醫療研討會。臺灣植物及樹木醫學學會。 17. 孫岩章。2018c。預防樹木褐根病及運用治療注射新技術專業施作之必要性。台灣林業 44(1):47-56。 18. 孫岩章。2021。植物醫師與樹木醫師培訓的建議。農業世界雜誌454:31-37。 19. 孫岩章、李綺璇。2020。於水瓊脂上塗佈離體病菌進行藥劑藥效測試之方法。2020植栽及樹木之健檢與醫療研討會。臺灣植物及樹木醫學學會。 20. 孫岩章、曾勝志、林立偉、謝譯賢。2019。群聚型樹木褐根病在田間以掘溝配合藥劑注射防治成功之實例。2019植醫樹醫健檢醫療暨樹木褐根病研討會。臺灣植物及樹木醫學學會。 21. 孫岩章、黎志華、林炳松、陳添宇、傅學俞。2020。高速公路沿路樹木之健檢及風險評估之推展。2020植栽及樹木之健檢與醫療研討會。臺灣植物及樹木醫學學會。 22. 張東柱、謝煥儒、張瑞璋、傅春旭。1999。台灣常見樹木病害。林業叢刊。98號。203頁。 23. 張東柱、傅春旭。2017。樹病學。五南圖書出版公司。 24. 張廣淼、彭淑貞、吳添益。2009。國產優良品牌草莓生產管理技術作業標準。行政院農業委員會苗栗區農業改良場。 25. 黃晉興。2009。白粉病。植物保護圖鑑系列19─ 甜瓜保護。行政院農業委員會動植物防疫檢疫局。75-78頁。 26. 陳文華、曾勝志、陳學弘、蔡鎮宇、孫岩章。2015。台灣綠化樹種百大疫病蟲害之評選。樹木疫病蟲害之醫療及健檢研討會論文集。臺灣植物及樹木醫學學會。 27. 陳文華、孫岩章。2020。臺灣植物及樹木醫學學所提「樹木醫師培訓規範」之介紹。2020植栽及樹木之健檢與醫療研討會。臺灣植物及樹木醫學學會。 28. 陳文華。2009。蟲害及?害各論。植物保護圖鑑系列 19 ─ 甜瓜保護。行政院農業委員會動植物防疫檢疫局。12-56頁。 29. 陳彥佑、余思葳、楊秀珠。2016。百香果整合管理。行政院農業委員會農業藥物毒物試驗所。48頁。 30. 梁臻穎。2018。颱風造成臺北地區樹木風倒害因及其管理之研究。國立台灣大學植物醫學碩士學位學程碩士論文。 31. 梁臻穎、孫岩章。2018。颱風造成樹木倒伏害因之分析及預防。台灣林業 44(1):35-46。 32. 傅春旭、張東柱、李鎧彤、莊鈴木、黃勁暉。2016。以ITS分子序列診斷樹木靈芝病害。中華林學季刊 49(3):201-209。 33. 費雯綺、王喻其。2007。植物保護手冊─糧食作物及其他篇。412頁。第115頁。 34. 楊秀珠、余思葳。2012。十字花科蔬菜之病蟲害發生與管理。蔬菜病蟲害綜合防治專輯-蔬菜。行政院農業委員會藥物毒物試驗所。62頁。 35. 趙治平。2008。香蕉黑星病。植物保護圖鑑系列18-香蕉保護。行政院農業委員會動植物防疫檢疫局。180頁。46-48頁。 36. 蔡志濃、安寶貞、謝文瑞。2005。抑制褐根病菌、白紋羽菌及南方靈芝菌之化學藥劑篩選。植物病理學會刊 14:115-124。 37. 蔡志濃、鄭秀芳、蔡惠玲、黃鴻章、謝文瑞、安寶貞。2013。利用薄膜培養基法檢測褐根病菌分泌之生體外分解酵素。台灣農業研究 62(2):184-194。 38. 蔡志濃、謝文瑞、安寶貞。2008。氮肥與化學藥劑對果樹褐根病菌之影響與田間病害防治。植物病理學會刊 17:119-126。 39. 廖君達、郭建志。2011。引起馬拉巴栗基腐病之Fusarium solani之鑑定與防治。科技計畫研究成果發表會論文輯。95-100頁。 40. 黎志華。2021。櫻花及常見樹木疫病蟲害診治及風險評估之研究。國立台灣大學植物醫學碩士學位學程碩士論文。 41. 鐘珮哲、賴巧娟、蔡正賢、吳岱融。2020。草莓重要病蟲害辨識指南。行政院農業委員會苗栗區農業改良場。33頁。 42. 蕭文偉、孫岩章。2001。老樹病因的診斷與病例報告。巨木(老樹)保護研討會論文集。國立台灣大學農學院實驗林管理處。95-104頁。 43. 蕭文偉、孫岩章。2007。木棉老樹外科手術救治之研究。臺北市樹木保護推廣暨紀念謝副教授煥儒研討會。國立臺灣大學植物病理與微生物學系。台北市。 44. Bari, E., Daryaei, M. G., Karim, M., Bahmani, M., Schmidt, O., Woodward, S., Ghanbary, M.A.T.,and Sistani, A. 2019. Decay of Carpinus betulus wood by Trametes versicolor—An anatomical and chemical study. International Biodeterioration and Biodegradation 137: 68–77. 45. Behera, B. C., Parida, S., Dutta, S. K., and Thatoi, H. N. 2014. Isolation and identification of cellulose degrading bacteria from Mangrove soil of Mahanadi river delta and their cellulase production ability. Amer. J. Microbiol. Res. 2(1): 41–46. 46. Chang, T. T. 1995. A selective medium for phellinus noxins. European Journal of Forest Pathology 25: 185-190. 47. Chaparro, D. F., Rosas, D. C., and Varela, A. 2009. Isolation of wood-decaying fungi and evaluation of their enzymatic activity. Revista Iberoamericana de Micolog?a 26(4): 238-243. 48. Demissie, Z. A., Brown. W. G., Loewen, M. C. 2019. A universally primed-polymerase chain reaction (UP-PCR) marker to discriminate Clonostachys rosea ACM941 from related strains. Journal of Fungi 5(2): 39. 49. Dhingra, O. D. and Sinclair, J. B. 1995. Basic Plant Pathology Methods. CRC Press. 50. Fairmaire, L. 1889. Col?opt?res de l''int?rieur de la Chine 5e partie. Annales de la Soci?t? Entomologique de France 9(6): 5-84. 51. Fukasawa, Y. 2021. Ecological impacts of fungal wood decay types: A review of current knowledge and future research directions. Ecological Research 36(6): 910-931. 52. Fukasawa, Y., Osono, T., and Takeda, H. 2011. Wood decomposing abilities of diverse lignicolous fungi on nondecayed and decayed beech wood. Mycologia 103(3): 474-482. 53. Fukasawa, Y. 2018. Temperature effects on hyphal growth of wood-decay basidiomycetes isolated from Pinus densiflora deadwood. Mycoscience 59: 259– 262. 54. Ginterov?, A., and Janotkov?, O. 1975. A simple method of isolation and purification of cultures of wood-rotting fungi. Folia Microbiol (Praha). 20(6): 519-520. 55. Gonthier, P., and Nicolotti, G. 2013. Infectious forest diseases. CABI. University of Torino. 56. Goodell, B., Qian, Y., and Jellison, J. 2008. Fungal decay of wood : Soft rot-brown rot-white rot. In Development of Commercial Wood Preservatives. ACS Symposium Series. American Chemical Society. 57. Guglielmo, F., Bergemann, S., Gonthier, P., Nicolotti, G., and Garbelotto, M. 2007. A multiplex PCR-based method for the detection and early identification of wood rotting fungi in standing trees. Journal of Applied Microbiology 103: 1490-1507. 58. Hankin, L., and Anagnostakis, S. L. 1975. The use of solid media for detection of enzyme production by fungi. Mycologia 67: 597-607 59. Hankin, L., and Anagnostakis, S. L. 1977. Solid media containing carboxymethylcellulose to detect Cx cellulase activity of micro-organisms. Journal of General Microbiology 98: 109-115. 60. Hartman, J. R., Pirone, T. P., and Sall, M. A. 2000. Pirone's tree maintenance (7th ed.). New York: Oxford University Press. 61. Hsiao, W. W., Hung, T. H., and Sun, E. J. 2019. Newly discovered basidiocarps of Phellinus noxius on 33 tree species with brown root rot disease in Taiwan and the basidiospore variations in growth rate. Taiwania 64(3): 97-102. 62. Hsiao, W. W., Sun, E. J., Wu, R. Y., and Hung, T. H. 2020. Malabar chestnut as a model and bioassay plant for Phellinus noxius brown root rot disease. J. Plant Med. 62: 17-24. 63. Jasalavich, C. A., Ostrofsky, A., and Jellison, J. 2000. Detection and identification of decay fungi in spruce wood by restriction fragment length polymorphism analysis of amplified genes encoding rRNA. Appl. Environ. Microbiol. 66(11): 4725-4734. 64. Latinovic, J., Radisek, S. and Latinovic, N. 2015. Severe infection of figs by fig rust pathogen Cerotelium fici in Montenegro. Agric. Forest. 61: 101-107. 65. Lindblad, I. 2000. Host specificity of some wood-inhabiting fungi in a tropical forest. Mycologia 92(3): 399-405. 66. Martin, J. H., Mifsud, D., and Rapisarda,C. 2000. The whiteflies (Hemiptera: Aleyrodidae) of Europe and the Mediterranean Basin. Bulletin of Entomological Research 90: 407–448. 67. More, S. S., Renuka, P. S., Pruthvi, K., Swetha, M., Malini, S., and Veena, S. M. 2011. Isolation, purification, and characterization of fungal laccase from Pleurotus sp. Enzyme Res. 2011: 248735. 68. Morris, H., Hietala, A. M., Jansen, S., Ribera, J., Rosner, S., Salmeia, K. A., and Schwarze, F. W. M. R. 2020. Using the CODIT model to explain secondary metabolites of xylem in defense systems of temperate trees against decay fungi. Annals of Botany 125(5): 701–720. 69. M?ller, J., Ulyshen, M., Seibold, S., Cadotte, M., Chao, A., B?ssler, C., Vogel, S., Hagge, J., Weib, I., Baldrian, P., Tl?skal, V., and Thorn, S. 2020. Primary determinants of communities in deadwood vary among taxa but are regionally consistent. OIKOS 129: 1579–1588. 70. Pandey, K. K., and Pitman, A. J. 2003. FTIR studies of the changes in wood chemistry following decay by brown-rot and white-rot fungi. Int. Biodeter. Biodegr. 52: 151-160. 71. Potyralska, A., Schmidt, O., Moreth, U., Lakomy, P., and Siwecki, R. 2002. rDNA-ITS sequence of Armillaria species and a specific primer for A. mellea. Forest Genetics 9: 119-123 72. Reynolds, N. K., Jusino, M. A., Stajich, J. E., and Smith, M.E. 2021. Understudied, underrepresented, and unknown: Methodological biases that limit detection of early diverging fungi from environmental samples. Mol. Ecol. Resour. 22(3): 1065-1085. 73. Sazci, A., Radford, A., and Erenler, K. 1986. Detection of cellulolytic fungi by using Congo red as an indicator: A comparative study with the dinitrosalicyclic acid reagent method. Journal of Applied Bacteriology 61: 559-562. 74. Schilling, M., Farine, S., Peros, J. -P., Bertsch, C., and Gelhaye, E. 2021. Wood degradation in grapevine diseases. Advances in Botanical Research 99: 175-207. 75. Schmit, J. P. 2005. Species richness of tropical wood-inhabiting macrofungi provides support for species-energy theory. Mycologia 97(4): 751-761. 76. Schubert, M., Fink, S., and Schwarze, F.W.M.R. 2008. Evaluation of Trichoderma spp. as a biocontrol agent against wood decay fungi in urban trees. Biological Control 45: 113-123 77. Shigo, A.L. 1985. Compartmentalization of decay in trees. Scientific American 152 (4): 96-103. 78. Sun, E. J., Chen, W. H., Li, W. C., and Huang, R. N. 2020. Shallow-hole trunk and root injection with a fungicide-containing mixture for controlling brown root rot disease in trees. J. Plant Med. 62(2): 25-34. 79. Szabo, L. J., Mollov, D. S., Rosen, C. 2013. First report of garlic rust caused by Puccinia allii on Allium sativum in minnesota. Plant Dis. 97(2): 285. 80. Thomas, L., Ram, H., and Singh, V. 2018. Inducible cellulase production from an organic solvent tolerant Bacillus sp. SV1 and evolutionary divergence of endoglucanase in different species of the genus Bacillus. Brazilian Journal of Microbiology 49: 429-442. 81. Tischner, Z., P?ldy, A., Kocsub?, S., Kredics, L., Dobolyi, C., Seb?k, R., Kriszt, B., Szab?, B., Magyar, D. 2022. Survival and growth of microscopic fungi derived from tropical regions under future heat waves in the Pannonian Biogeographical Region. Fungal Biology 126(8): 511-520. 82. To-anun, C., Visarathanonth, N., Engkhaninun, J., and Kakishima, M. 2004. First report of plumeria rust, caused by Coleosporium plumeriae, in Thailand. The Natural History Journal of Chulalongkorn University 4(1): 41-46. 83. Weaver, D. J., 1974. A gummosis disease of peach trees caused by Botryosphaeria dothidea. Phytopathology 64: 1429-1432. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/83507 | - |
dc.description.abstract | 台大植物醫學碩士學位學程最主要目的是培養出能夠服務農民、改善農業及樹木產業的植醫及樹醫專業人才。本論文以此為目標分為兩大部分:一是植醫及樹醫相關診治案例實作記錄及報告;二是城巿樹木傷口棲息菌之研究。 重要植物疫病蟲害診治案例包含雲林、屏東、台北等地區之農業病蟲害案例與台北地區之樹木診治病例。如銹病、白粉病、灰黴病、炭疽病等病害,並使用水瓊脂平台法之藥劑測試方法尋求最佳之處方。蚜蟲、?類、粉蝨等蟲害則多使用葉面噴施之方法進行防治之測試。樹木之褐根病及靈芝根基腐等係以莖部淺孔注射作為醫療方法;松樹之松斑天牛以因滅汀與芬普尼進行莖部注射;櫻花流膠以待克利主劑進行流膠部位之注射。另樹木之傷口腐朽則以外科手術進行清創、藥劑之除菌除蟲,再經抗風性RC修補及表面修復。各診治案例皆有藥劑測試之結果與診治後之改善狀況及未來之建議,為日後植醫及樹醫服務累積一定之診治能力及經驗。 樹木傷口棲息菌之研究以採集台北地區之八種常見公園及行道樹種之高部位修剪傷口樣本進行分離,包括艷紫荊、台灣欒、阿勃勒、青剛櫟、光蠟樹、榕樹、樟樹及台灣櫸。各以四種培養基,包括馬鈴薯葡萄糖瓊脂培養基(PDA)、酸化PDA、麥芽瓊脂培養基(MEA)及MA+4選擇性培養基進行組織分離及菌量測定。共分離出110個菌株,經固體培養基之體外分泌酵素能力測定,得知其中54個菌株同時具有纖維素與木質素分解能力,可能為軟腐菌或白腐菌。另23個菌株只有纖維素分解能力;9個菌株只有木質素分解能力。經ITS與LSU序列進行分子鑑定與形態對比,54個菌株中以子囊菌佔大多數,達85%。又自54個菌株中,挑選較好培養的12個菌株進行3種樹木木片培養之木材腐朽能力測試,經培養30日後可測得其乾重損失率即為木材腐朽率。結果發現屬於擔子菌之2菌株木片乾重損失率較高達14-16%,子囊菌者則木片乾重損失率約為6-8%,即亦有相當的木片腐朽能力,並能在木片上長出產孢結構。 自八種常見公園及行道樹種之高部位修剪傷口進行組織分離、鑑定及菌量測定之結果,發現除了台灣櫸以外,其他7種之傷口棲息菌中,皆以子囊菌為主流,其種數約佔90%,總菌量也佔9成以上,唯在台灣櫸中,子囊菌種數只佔6成,菌量則佔8成以上,種數之多寡與菌量兩者是造成傷口腐朽程度之關鍵。 本研究挑選54個菌株中之7個菌株(SR01、SR02、SR10、SR18、SR24、SR30、SR35)進行藥劑測試,選用24.9%待克利乳劑、75%四氯異苯?可濕性粉劑、25%普克利乳劑、50%蓋普丹可濕性粉劑、25%三泰芬可濕性粉劑、50倍波爾多液。使用含藥培養基與PD3A平台上菌液與藥劑直接接觸之兩種方法進行測試。發現含藥培養基之測試法易受藥劑劑型的影響,菌液與藥劑直接接觸之藥劑測試法則較便利及與可信。結果發現待克利與普克利對於分離之樹木棲息菌有較好之抑制效果,抑制率可達100%和95%。三泰芬於擔子菌有較高於子囊菌之抑制率。而對新北巿新莊林道於2018年施用三泰芬石灰合劑進行傷口保護之腐朽程度調查,發現其腐朽程度皆屬輕度,可以證明其具腐朽預防之藥效。 | zh_TW |
dc.description.abstract | The main purpose of the Master Program for Plant Medicine of National Taiwan University is to train professional plant doctor and tree doctor who can serve farmers and the tree industry to improve the plant health. This dissertation is divided into two parts for this purpose: the first is the 50 case reports each with diagnosis and treatment of important plant diseases, tree pests or non-infectious plant diseases; the second is the in-depth research on tree wound inhabiting fungi in urban forest. The important case reports of diagnosis and treatment include crop diseases and insect pests cases in Yunlin, Pingtung, Taipei and other areas and tree diagnosis and treatment cases in Taipei or other area. Major cases are rust, powdery mildew, gray mold, and anthracnose etc. The study uses the water agar platform method to screen the best agent for controlling the pathogens. Aphids, mites, whiteflies and other pests are controlled by conventional foliar spraying. The brown root rot and Ganoderma root and butt rots were cured by shallow-hole trunk injection with difenoconazole as principal fungicide. The Whu-she blood-spotted long-horned Beetle was treated by injecting emamectin benzoate or fipronil as principal insecticide into the stem. The cherry gummosis disease was cured by injecting principal difenoconazole into the gumming points of tree stem. Tree wounds are surgically treated by wound-cleaning, pesticide application to remove the wood rot fungi and insects, then refilled with wind-resistant RC structure and remodelled with natural tree bark. Each diagnosis and treatment case has the results of the controlling test or improving situation and the recommendation for future works, which will accumulate certain diagnosis and treatment ability or experience for the future plant medicine and tree medicine services. For the study of tree wound inhabiting fungi, pruning wound samples from eight common tree species in Taipei areas were investigated, collected and the containing fungi were isolated. They are Hong Kong orchid tree, flame gold-rain tree, golden shower tree, ring-cupped oak, Formosan ash, small-leafed banyan, camphor tree, and Taiwan zelkova. Four culturing media including the potato dextrose agar (PDA), acidified PDA, malt agar medium, and MA+4 selective medium were used for isolating all the available fungi in the wounds. A total of 110 fungal isolates were obtained, among them 54 isolates showing decomposition abilities for both cellulose and lignin, similar to the white rot or soft rot fungi. While 23 isolates can decompose only the cellulose, and 9 isolates can decompose only the lignin. Molecular identification by PCR and ITS or LSU sequencing together with morphological comparison of the 54 soft rot isolates showed that Ascomycetes groups accounted for 85% of them. The major 12 isolates from these 54 ones were cultured on sterilized wood chips of three tree species for 30 days to determine their wood decomposition ability. Results showed that two Basidiomycetes isolates can decompose the wood chip with about 14-16% dry weight loss, while the major Ascomycetes isolates can decompose the wood chip with about 6-8% dry weight loss, expressing their realistic wood rotting ability. From the isolation and identification results of wound rot fungi on eight common tree species in Taipei areas, we find that Ascomycetes fungi are the dominant group, occupying 90 % of the fungal species, except on the Taiwan zelkova tree wounds which is only 60%. On the basis of propagule density in wound, the Ascomycetes fungi are also the dominant group, occupying over 90 % of the propagule, even on the Taiwan zelkova tree wounds is over 80%. The species and propagule dominance of Ascomycetes fungi in tree wounds indicate that they play very important roles in wound rotting in the urban tree ecosystem. Among the 54 soft rot isolates, 7 of them (SR01, SR02, SR10, SR18, SR24, SR30, SR35) were selected for fungicide testing. Six fungicide including 24.9% difenoconazole, 75% chlorothalonil WP, 25% propiconazole, 50 % captan WP, 25% triadimefon WP, and Bordeaux mixture (50X), were selected for the experiments. Two methods were conducted in the test, including the poisoned food medium method and the PD3A platform method in which the fungal mycelial suspension is directly mixed with the fungicide on the harder PDA platform. Results showed that the fungal response in poisoned food medium could be affected by the formulation type of the fungicide. While the PD3A platform method showed the more convenient and reliable results. From the testing we find that difenoconazole and propiconazole have better inhibitory effect on most of the test isolates, with inhibition rate reaching 100% and 95%, respectively. The triadimefon showed a higher inhibition ability to Basidiomycetes isolates than the Ascomycetes isolates. A special investigation of wound protectant field trial at Shin-Chung district, New Taipei City, showed that the application of triadimefon-lime mixture on pruned wounds in 2018 expressed good protection results so far as the wound rot level are lower than those without the protectant. | en |
dc.description.provenance | Made available in DSpace on 2023-03-19T21:09:11Z (GMT). No. of bitstreams: 1 U0001-1708202221190400.pdf: 17509849 bytes, checksum: 996b31509c6bb00c9e1fd2101c1c6212 (MD5) Previous issue date: 2022 | en |
dc.description.tableofcontents | 目錄 第一章 前言 1 第二章 前人研究 3 一、 植醫及樹醫相關案例之診斷及防治 3 二、 木材腐朽菌簡介 4 三、 木材棲息菌之分離與鑑定 5 四、 木材分解能力測定 6 五、 樹木傷口之藥劑保護 7 第三章 材料與方法 8 一、 重要植物醫學疫病蟲害五十診治案例報告 8 二、 常見樹木傷口腐朽之調查及棲息菌之分離 9 (一) 常見樹木修剪傷口腐朽之田間調查 9 (二) 樹木傷口木材棲息菌之採樣 10 (三) 樹木傷口木材棲息菌之分離 10 三、 木材棲息菌之分解能力測試 11 (一) 纖維素分解酵素分泌能力測試 11 (二) 木質素分解酵素分泌能力測試 11 (三) 木材棲息菌以木片培養之腐朽能力測試 12 四、 樹木傷口木材棲息菌之菌種鑑定 12 (一) 木材棲息菌之形態學鑑定 12 (二) 木材棲息菌之分子生物學鑑定 13 五、 城巿樹木傷口木材棲息菌之種類分佈 13 (一) 各樹種傷口棲息菌菌種及菌量之估算 13 (二) 傷口棲息菌菌種中子囊菌與擔子菌之佔比估算 14 六、 樹木傷口腐朽防治藥劑之篩選與測試 14 (一) 以培養基進行化學藥劑抑制棲息菌生長能力之測試 14 (二) 化學藥劑半抑制濃度(IC50)測試 14 (三) 田間預防性施用傷口保護劑之成效調查 15 第四章 結果 16 一、 重要植物醫學疫病蟲害五十診治案例報告 16 二、 常見樹木傷口腐朽之調查及棲息菌之分離 68 (一) 常見樹木修剪傷口腐朽之田間調查 68 (二) 常見樹木傷口棲息菌之採樣及分離 68 三、木材棲息菌之分解能力測試 76 (一) 纖維素分解酵素分泌能力測試 76 (二) 木質素分解酵素分泌能力測試 77 (三) 纖維素與木質素分解能力測試之總合結果 78 (四) 木材棲息菌之腐朽能力測試 82 四、 樹木傷口木材棲息菌之初步鑑定 86 五、 城巿樹木傷口木材棲息菌之種類分佈 90 (一) 各樹種傷口棲息菌菌種及菌量之估算 90 (二) 傷口棲息菌菌種中子囊菌與擔子菌之佔比估算 95 六、 樹木傷口腐朽防治藥劑之篩選與測試 95 (一) 以培養基進行化學藥劑抑制能力之測試 95 (二) 化學藥劑半抑制濃度(IC50)測試 101 (三) 田間預防性施用傷口保護劑 104 第五章 討論 106 一、 重要植物醫學疫病蟲害五十診治案例報告 106 二、 樹木傷口棲息菌之研究 107 參考文獻 110 ? 表目錄 表1. 台北地區採集傷口木屑樣本之常見樹種樹木調查資料 69 表2. CMC澄清環澄清度之分級標準 76 表3. ABTS氧化程度之分級標準 77 表4. 自樹木傷口分離之菌株培養五天同時具有纖維素分解能力與木質素分能能力的測定結果 79 表5. 自樹木傷口分離之菌株培養五天只具有纖維素分解能力測定結果 80 表6. 自樹木傷口分離之菌株培養五天只具有木質素分解能力測定結果 81 表7.接種12個木材棲息菌之3種木片含水率 83 表8. 接種12個木材棲息菌培養30天之木片乾重損失率 84 表9. 同時具有纖維素與木質素分解之木材棲息菌分子鑑定結果 86 表10. 艷紫荊傷口以4種培養基分離所得之主要菌株及其菌量結果 91 表11. 台灣欒傷口以4種培養基分離所得之主要菌株及其菌量結果 91 表12. 阿勃勒傷口以4種培養基分離所得之主要菌株及其菌量結果 92 表13. 青剛櫟傷口以4種培養基分離所得之主要菌株及其菌量結果 92 表14. 光蠟樹傷口以4種培養基分離所得之主要菌株及其菌量結果 93 表15. 榕樹傷口以4種培養基分離所得之主要菌株及其菌量結果 93 表16. 樟樹傷口以4種培養基分離所得之主要菌株及其菌量結果 93 表17. 台灣櫸傷口以4種培養基分離所得之主要菌株及其菌量結果 94 表18. 8種樹木傷口中同時具有纖維素與木質素分解能力之子囊菌與擔子菌之佔比分析結果 95 表19、測試5種化學藥劑以100ppm之濃度及50倍之波爾多液加入PDA培養基對七種木材棲息菌菌絲生長之抑制結果 97 表20、測試5種化學藥劑以100ppm之濃度及50倍之波爾多液直接在PD3A上與七種木材棲息菌菌絲液接觸對生長之抑制結果 98 表21、待克利與普克利對七種木材棲息菌於PD3A平台測試之半抑制濃度(IC50)結果 102 圖目錄 圖1. 木材棲息菌於CMC培養基培養以剛果紅染色之結果 77 圖2. 移離YMEA培養基已括除菌絲並以ABTS染色之松木木片 78 圖3. 木材棲息菌接種於木材木片三十天之生長狀況 83 圖4. 12種木材棲息菌接種於三種木材木片三十天導致之平均乾重損失率 84 圖5. 12種木材棲息菌接種於三種木片木材培養三十天刮除菌絲後烘乾之木片形態 85 圖6. 木材棲息菌菌株於PDA之形態特徵 88 圖7. SR01與6種化學藥劑直接接觸五天之抑制結果 99 圖8. SR10與6種化學藥劑直接接觸五天之抑制結果 99 圖9. SR24與6種化學藥劑直接接觸五天之抑制結果 100 圖10. SR30與6種化學藥劑直接接觸五天之抑制結果 100 圖11. SR35與6種化學藥劑直接接觸五天之抑制結果 101 圖12. SR10與6種濃度化學藥劑在PD3A平台直接接觸五天之抑制結果 103 圖13. SR18與6種濃度化學藥劑在PD3A平台直接接觸五天之抑制結果 103 圖14. SR30與6種濃度化學藥劑在PD3A平台直接接觸五天之抑制結果 104 | |
dc.language.iso | zh-TW | |
dc.title | 植物醫學重要診治案例及樹木傷口棲息菌之研究 | zh_TW |
dc.title | Important diagnosis and treatment cases in plant medicine and research on tree wound inhabiting fungi | en |
dc.type | Thesis | |
dc.date.schoolyear | 110-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 洪挺軒(Ting-Hsuan Hung) | |
dc.contributor.oralexamcommittee | 鍾嘉綾(Chia-Lin Chung),張東柱(Tun-tschu Chang) | |
dc.subject.keyword | 診治案例,修剪傷口,樹木注射,水瓊脂平台,木材腐朽菌,軟腐菌, | zh_TW |
dc.subject.keyword | diagnosis and treatment cases,pruning wound,shallow-hole trunk injection,water agar platform,wood decay fungi,soft-rot fungi, | en |
dc.relation.page | 115 | |
dc.identifier.doi | 10.6342/NTU202202526 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2022-09-05 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物醫學碩士學位學程 | zh_TW |
顯示於系所單位: | 植物醫學碩士學位學程 |
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