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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王錦堂(Jin-Town Wang) | |
| dc.contributor.author | Ming-Chen Chiang | en |
| dc.contributor.author | 江明真 | zh_TW |
| dc.date.accessioned | 2022-11-25T08:01:13Z | - |
| dc.date.copyright | 2021-08-31 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-08-11 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/82862 | - |
| dc.description.abstract | "心血管疾病是全球一大死因，近年來已經有許多論文報導它的致病機制其中之一是由於腸道菌產生的小分子代謝物導致。當人類攝食動物性食物，例如：紅肉、雞蛋、貝類以及魚類等富含膽鹼 (choline) 和左旋肉鹼 (L-carnitine)，這些物質會被一些腸道菌分解產生三甲胺 (trimethylamine, TMA)，而三甲胺藉由肝臟酵素形成氧化三甲胺 (trimethylamine-N-oxide, TMAO) 進入血液循環，若氧化三甲胺累積濃度高可能導致心血管疾病的發生。本研究室的合作團隊利用16S 核醣體DNA次世代定序分析血漿中有高濃度氧化三甲胺的受試者之腸道菌株，其中與產氧化三甲胺高度相關的菌株包含Alistipes timonensis、埃氏類桿菌 (Bacteroides eggerthii)和Ihubacter massiliensis，因此本研究利用受試者糞便檢體培養腸道微生物及聚合酶連鎖反應技術進行分離A. timonensis、B. eggerthii和I. massiliensis。實驗結果顯示無法分離出A. timonensis，B. eggerthii則在糞便檢體O13中成功分離，並將其命名為B. eggerthii O13，而I. massiliensis仍在分離中。之後對B. eggerthii O13進行膽鹼、肉鹼及γ-丁基甜菜鹼 (γ-Butyrobetaine, γ-BB) 之代謝利用實驗，結果顯示B. eggerthii O13不具有代謝膽鹼、肉鹼或γ-丁基甜菜鹼產生三甲胺的功能。本研究另探討先前由本實驗室分離出可能為低三甲胺生成相關的菌株Flavonifractor plautii是否對代謝γ-丁基甜菜鹼為三甲胺的Emergencia timonensis具抑制能力，將兩種菌株共同培養進行γ-丁基甜菜鹼代謝利用競爭實驗，結果顯示雖然F. plautii可能藉由抑制E. timonensis生長而降低三甲胺生成，但不具有顯著差異。" | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-25T08:01:13Z (GMT). No. of bitstreams: 1 U0001-1008202117075700.pdf: 3354599 bytes, checksum: f08f409d9127de87698ed395110b07c3 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | "口試委員會審定書 i 誌謝 ii 中文摘要 iii Abstract iv 目錄 v 表目錄 vii 圖目錄 viii 第一章、緒論 1 1.1 腸道菌叢 (Gut microbiota) 1 1.2 心血管疾病 (Cardiovascular disease, CVD) 1 1.3 腸道菌與心血管疾病關聯 2 1.4 具產高三甲胺能力潛在對象 3 1.5 Alistipes timonensis 4 1.6 Bacteroides eggerthii 5 1.7 Ihubacter massiliensis 5 1.8 Flavonifractor plautii 5 1.9 研究動機 (Aim) 6 第二章、材料與方法 7 2.1 材料 7 2.1.1 糞便檢體 7 2.1.2 菌株 (Strain) 7 2.1.3 培養基 (Media) 7 2.1.4 引子 (Primer) 7 2.1.5 化合物 (Compound) 8 2.2 方法 8 2.2.1 菌株培養 8 2.2.2 糞便之腸道微生物培養 8 2.2.3 糞便之基因組DNA萃取 9 2.2.4 細菌之基因組DNA萃取 9 2.2.5 聚合酶連鎖反應 (Polymerase chain reaction, PCR) 10 2.2.6 菌落聚合酶連鎖反應 (Colony polymerase chain reaction, colony PCR) 11 2.2.7 分離特定腸道菌種 11 2.2.8 分離I. massiliensis 12 2.2.9 含I. massiliensis菌液管餵無菌鼠 13 2.2.10 氘標定之膽鹼及肉鹼之代謝利用實驗 13 2.2.11 氘標定之γ-丁基甜菜鹼之代謝利用實驗 13 2.2.12 氘標定γ-丁基甜菜鹼之代謝利用競爭實驗 14 第三章、結果 15 3.1以菌落聚合酶連鎖反應分離菌種 15 3.1.1 Alistipes timonensis的分離 15 3.1.2 Bacteroides eggerthii的分離 15 3.1.3 Ihubacter massiliensis的分離 16 3.2 膽鹼、肉鹼和γ-丁基甜菜鹼之代謝利用實驗 17 3.3 Alistipes timonensis與其他腸道微生物cutC及cutD 基因比對 17 3.3.1 cutC序列比對 17 3.3.2 cutD序列比對 18 3.4 Flavonifractor plautii與Emergencia timonensis交互作用 18 3.4.1 γ-丁基甜菜鹼之代謝利用實驗 18 3.4.2 競爭實驗 19 第四章、討論 20 第五章、參考文獻 22" | |
| dc.language.iso | zh-TW | |
| dc.title | 分離產高三甲胺表現型之相關菌種與其功能分析 (II) | zh_TW |
| dc.title | Isolation and functional analysis of bacterial species associated with high trimethylamine producing phenotype (II) | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 賴信志(Hsin-Tsai Liu),魏淑鉁(Chih-Yang Tseng) | |
| dc.subject.keyword | 心血管疾病 (Cardiovascular disease, CVD),腸道菌叢 (gut microbiota),三甲胺 (trimethylamine, TMA),Bacteroides eggerthii,Flavonifractor plautii, | zh_TW |
| dc.subject.keyword | Cardiovascular disease,Gut microbiota,trimethylamine,Bacteroides eggerthii,Flavonifractor plautii, | en |
| dc.relation.page | 52 | |
| dc.identifier.doi | 10.6342/NTU202102250 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2021-08-12 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 微生物學研究所 | zh_TW |
| dc.date.embargo-lift | 2024-08-31 | - |
| 顯示於系所單位： | 微生物學科所 | |
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