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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林順福(Shun-Fu Lin) | |
dc.contributor.author | Tzu-Kai Lin | en |
dc.contributor.author | 林子凱 | zh_TW |
dc.date.accessioned | 2021-05-20T00:51:03Z | - |
dc.date.available | 2023-08-11 | |
dc.date.available | 2021-05-20T00:51:03Z | - |
dc.date.copyright | 2020-08-21 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2020-08-12 | |
dc.identifier.citation | 何余堂、陳寶元、傅廷棟、李殿榮、涂金星 (2003) 白菜型油菜在中國的起源與進化。遺傳學報 30 :1003-1012。 宋來強、傅廷棟、楊光聖、涂金星、馬朝芝 (2005) 1 對複等位基因控制的油菜( Brassica napus L.) 顯性核不育系609AB的遺傳驗証。作物學報 31:869-875。 李殿榮 (1980) 甘藍型油菜三系選育初報。陝西農業科學 (1):26-29。 林俊義 (2006) 能源作物之國外推動經驗與國內發展展望(下)。技術服務 65:5-10。 邱祥發、彭武男 (1995) 低芥酸油菜品種之育種研究。桃園區農業改良場研究彙報第22號。 胡勝武、于澄宇、趙惠賢、路明、張春紅、俞延軍 (2004) 甘藍型油菜核不育材料Shaan-GMS恢復基因的篩選及其遺傳分析。西北農林科技大學學報(自然科學版) 32:9-18。 張慧、張淑江、武劍、李菲、章時蕃、王曉武、張新梅、孫日飛 (2010) 大白菜細胞核隱性雄性不育系恢復基因BrMf2的標記及定位. 中國農業科學 43 :993-999。 曹家樹、曹壽椿 (1995) 大白菜起源的雜交驗證初報。園藝學報 22 :93-94。 曹壽椿、李式軍 (1982) 白菜地方品種的初步研究—Ⅲ.不結球白菜品種的園藝學分類。南京農業大學學報 (2):30-37。 郭晶心、周乃元、馬榮才、曹鳴慶 (2002) 白菜類蔬菜遺傳多樣性的AFLP分子標記研究. 農業生物技術學報 10:138-143。 馮輝、魏毓棠、許明 (1995) 大白菜核基因雄性不育系遺傳假說及其驗證。中國科協第二屆青年學術年會園藝論文集pp 458-466。 楊光巠、傅廷棟 (1998) 甘藍型油菜細胞質雄性不育的遺傳分類研究。中國農業科學 31:27-31。 董振生、劉創社、景軍勝、冉龍貴、張修森、董軍剛、劉詢俠 (1999) 白菜型油菜雙顯性核不育896AB恢復系基因型的鑑定。作物學報 25 :193-198。 劉后利 (1984) 幾種蕓薹屬油菜的起源和進化。作物學報 10:9-18。 劉定富 (1992) 植物顯性核不育恢復性遺傳的理論探討。遺傳 14:31-36。 鄭仲孚、趙致康、吳國璋、林興 (1963) 台灣雜糧作物品種圖說。台灣省政府農林廳 pp 251-267。 魏大勇、譚傳東、 崔藝馨、吳道明、李加納、梅家琴、錢偉 (2017) 甘藍型油菜polCMS育性恢復位點的全基因組關聯分析。中國農業科學 50:802-819。 譚其猛 (1979) 試論大白菜品種的起源、分布和演化。中國農業科學 12 :68-75。 Ashikawa I, Hayashi N, Yamane H, Kanamori H, Wu J, Matsumoto T, Ono K, Yano M (2008) Two adjacent nucleotide-binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance. Genetics 180 :2267-2276. Bannerot H, Boulidard L, Cauderon Y, Tempe J (1974) Transfer of cytoplasmic male sterility from Raphanus sativus to Brassica oleracea. In: Wills AB, North C (Eds) Proc Eucarpia Meet Cruciferae. Scottish Hortic Res Inst, Invergavrie, UK, pp 52–54. Bannerot H, Boulidard L, Chupeau Y (1977) Unexpected difficulties met with the radish cytoplasm in Brassica oleracea. Eucarpia Cruciferae Newsletter 2: 16. Borkakati RR, Virmani SS (1996) Genetics of thermosensitive genic male sterility in rice. Euphytica 88:1-7. Broman KW (2010) Genetic Map Construction with R/qtl, pp. Technical Report # 214. University of Wisconsin-Madison, Department of Biostatistics Medical Informatics. Broman KW, Wu H, Sen Ś, Churchill GA (2003) R/qtl: QTL mapping in experimental crosses. Bioinformatics 19:889-890. Cao S, Li S (1981) Breeding of summer Chinese cabbage 'Dwarf Hybrid No. 1' and male sterile AB lines. Acta Horticulturae Sinica 8:35-42 Catchen JM, Amores A, Hohenlohe P, Cresko W, Postlethwait JH (2011) Stacks: building and genotyping loci De Novo from short-read sequences. G3: Genes Genomes Genetics 1:171-182. Chang C-W, Wang Y-H, Tung C-W (2017) Genome-wide single nucleotide polymorphism discovery and the construction of a high-density genetic map for melon (Cucumis melo L.) using genotyping-by-sequencing. Front Plant Sci 8:125. Chen F, Hu B, Li C, Li Q, Chen W, Zhang M (1998) Genetic studies on GMS in Brassica napus L: I. Inheritance of recessive GMS line 9012A. Acta Agronomica Sinica 24:431-438. Cheng F, Liu S, Wu J, Fang L, Sun S, Liu B, Li P, Hua W, Wang X (2011) BRAD, the genetics and genomics database for Brassica plants. BMC Plant Biol 11 :136. Choi S, Teakle G, Plaha P, Kim J, Allender C, Beynon E, Piao Z, Soengas P, Han T, King G, Barker G, Hand P, Lydiate D, Batley J, Edwards D, Koo D, Bang J, Park B-S, Lim Y (2007) The reference genetic linkage map for the multinational Brassica rapa genome sequencing project. Theor Appl Genet 115:777-792. Chung H, Jeong Y-M, Mun J-H, Lee S-S, Chung W-H, Yu H-J (2014) Construction of a genetic map based on high-throughput SNP genotyping and genetic mapping of a TuMV resistance locus in Brassica rapa. Mol Genet Genomics 289 :149-160. Delourme R, Bouchereau A, Hubert N, Renard M, Landry BS (1994) Identification of RAPD markers linked to a fertility restorer gene for the Ogura radish cytoplasmic male sterility of rapeseed (Brassica napus L.). Theor Appl Genet 88:741-748. Delourme R, Foisset N, Horvais R, Barret P, Champagne G, Cheung WY, Landry BS, Renard M (1998) Characterisation of the radish introgression carrying the Rfo restorer gene for the Ogu-INRA cytoplasmic male sterility in rapeseed (Brassica napus L.). Theor Appl Genet 97:129-134. Deng Z, Li X, Wang Z, Jiang Y, Wan L, Dong F, Chen F, Hong D, Yang G (2016) Map-based cloning reveals the complex organization of the BnRf locus and leads to the identification of BnRfb, a male sterility gene, in Brassica napus. Theor Appl Genet 129:53-64. Dong F, Hong D, Xie Y, Wen Y, Dong L, Liu P, He Q, Yang G (2012) Molecular validation of a multiple-allele recessive genic male sterility locus (BnRf) in Brassica napus L. Mol Breed 30:1193-1205. Elshire RJ, Glaubitz JC, Sun Q, Poland JA, Kawamoto K, Buckler ES, Mitchell SE (2011) A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species. PLoS ONE 6 (5):e19379. doi:10.1371/journal.pone.0019379 Engelke T, Gera D, Tatlioglu T (2004) Determination of the frequencies of restorer- and maintainer-alleles involved in CMS1 and CMS2 in German chive varieties. Plant Breed 123:51-59. Fan ZX, Lei WX, Hong DF, He JP, Wan LL, Xu ZH, Liu PW, Yang GS (2007) Development and primary genetic analysis of a fertility temperature-sensitive polima cytoplasmic male sterility restorer in Brassica napus. Plant Breed 126:297-301. Fang Z, Sun P, Liu Y, Yang L, Wang X, Hou A, Bian C (1997) A male sterile line with dominant gene (Ms) in cabbage (Brassica oleracea var. capitata) and its utilization for hybrid seed production. Euphytica 97 :265-268. Farfan IDB, De La Fuente GN, Murray SC, Isakeit T, Huang P-C, Warburton M, Williams P, Windham GL, Kolomiets M (2015) Genome Wide Association Study for Drought, Aflatoxin Resistance, and Important Agronomic Traits of Maize Hybrids in the Sub-Tropics. PLoS ONE 10:e0117737. doi:10.1371/journal.pone.0117737 Feng H, Wei P, Piao Z-Y, Liu Z-Y, Li C-Y, Wang Y-G, Ji R-Q, Ji S-J, Zou T, Choi S-R, Lim Y-P (2009) SSR and SCAR mapping of a multiple-allele male-sterile gene in Chinese cabbage (Brassica rapa L.). Theor Appl Genet 119:333-339. Feng H, Wei Y, Ji S, Jin G, Jin J, Dong W Multiple allele model for genic male sterility in Chinese cabbage. In: III International Symposium Diversification of Vegetable Crops 467, 1996. pp 133-138. FitzJohn RG, Armstrong TT, Newstrom-Lloyd LE, Wilton AD, Cochrane M (2007) Hybridisation within Brassica and allied genera: evaluation of potential for transgene escape. Euphytica 158:209-230. Fu T, Yang G, Yang X (1990) Studies on “Three Line” Polima Cytoplasmic Male Sterility Developed in Brassica napus L. Plant Breed 104:115-120. Ganal MW, Durstewitz G, Polley A, Bérard A, Buckler ES, Charcosset A, Clarke JD, Graner E-M, Hansen M, Joets J, Le Paslier M-C, McMullen MD, Montalent P, Rose M, Schön C-C, Sun Q, Walter H, Martin OC, Falque M (2011) A large maize (Zea mays L.) SNP genotyping array: development and germplasm genotyping, and genetic mapping to compare with the B73 reference genome. PLoS ONE 6:e28334. doi:10.1371/journal.pone.0028334 Hackett C, Broadfoot L (2003) Effects of genotyping errors, missing values and segregation distortion in molecular marker data on the construction of linkage maps. Heredity 90:33. Hall TA BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In: Nucleic Acids Symposium Series, 1999. vol 41. Information Retrieval Ltd., [London] c1979-c2000., pp 95-98. Hansen M, Halldén C, Nilsson N-O, Säll T (1997) Marker-assisted selection of restored male-fertile Brassica napus plants using a set of dominant RAPD markers. Mol Breed 3:449-456. Haslam TM, Haslam R, Thoraval D, Pascal S, Delude C, Domergue F, Fernández AM, Beaudoin F, Napier JA, Kunst L, Joubès J (2015) ECERIFERUM2-LIKE proteins have unique biochemical and physiological functions in very-long-chain fatty acid elongation. Plant Physiol 167:682-692. Hatzig SV, Frisch M, Breuer F, Nesi N, Ducournau S, Wagner MH, Leckband G, Abbadi A, Snowdon RJ (2015) Genome-wide association mapping unravels the genetic control of seed germination and vigor in Brassica napus. Front Plant Sci 6:221. doi:10.3389/fpls.2015.00221 He J, Ke L, Hong D, Xie Y, Wang G, Liu P, Yang G (2008) Fine mapping of a recessive genic male sterility gene ( Bnms3 ) in rapeseed ( Brassica napus ) with AFLP- and Arabidopsis -derived PCR markers. Theor Appl Genet 117:11-18 He YQ, Yang J, Xu CG, Zhang ZG, Zhang Q (1999) Genetic bases of instability of male sterility and fertility reversibility in photoperiod-sensitive genic male-sterile rice. Theor Appl Genet 99:683-693. Heng S, Shi D, Hu Z, Huang T, Li J, Liu L, Xia C, Yuan Z, Xu Y, Fu T, Wan Z (2015) Characterization and classification of one new cytoplasmic male sterility (CMS) line based on morphological, cytological and molecular markers in non-heading Chinese cabbage (Brassica rapa L.). Plant Cell Rep 34:1529-1537. Hong D, Wan L, Liu P, Yang G, He Q (2006) AFLP and SCAR markers linked to the suppressor gene (Rf) of a dominant genetic male sterility in rapeseed (Brassica napus L.). Euphytica 151:401-409. Hong DF, Liu J, Yang GS, He QB (2008) Development and characterization of SCAR markers associated with a dominant genic male sterility in rapeseed. Plant Breed 127:69-73. Huang L, Yang Y, Zhang F, Cao J (2017) A genome-wide SNP-based genetic map and QTL mapping for agronomic traits in Chinese cabbage. Sci Rep 7:46305. doi:10.1038/srep46305 Huang Z, Chen Y, Yi B, Xiao L, Ma C, Tu J, Fu T (2007) Fine mapping of the recessive genic male sterility gene ( Bnms3 ) in Brassica napus L. Theor Appl Genet 115:113-118. Imai R, Koizuka N, Fujimoto H, Hayakawa T, Sakai T, Imamura J (2003) Delimitation of the fertility restorer locus Rfk1 to a 43-kb contig in Kosena radish (Raphanus sativus L.). Mol Genet Genomics 269:388-394. Jean M, Brown GG, Landry BS (1997) Genetic mapping of nuclear fertility restorer genes for the ‘Polima’ cytoplasmic male sterility in canola (Brassica napus L.) using DNA markers. Theor Appl Genet 95:321-328. Kaul MLH (1988) Male sterility in higher plant. Monographs on Theoretical and Applied Genetics, vol 10. Springer-Verlag, Germany Ke LP, Sun YQ, Hong DF, Liu PW, Yang GS (2005) Identification of AFLP markers linked to one recessive genic male sterility gene in oilseed rape, Brassica napus. Plant Breed 124:367-370. Kenji W, Takashi S, Eimi F, Kenji K, Daizo I, Junzo F (2013) Genic male sterility in Brassica rapa ssp. rapa cv. 77B. J of Agri Sci 57 :287-292. Kim H, Choi SR, Bae J, Hong CP, Lee SY, Hossain MJ, Van Nguyen D, Jin M, Park B-S, Bang J-W, Bancroft I, Lim YP (2009) Sequenced BAC anchored reference genetic map that reconciles the ten individual chromosomes of Brassica rapa. BMC Genomics 10 (1):432. doi:10.1186/1471-2164-10-432 Kosambi DD (1943) The estimation of map distances from recombination values. Annals of Eugenics 12:172-175. Lee J, Izzah NK, Choi B-S, Joh HJ, Lee S-C, Perumal S, Seo J, Ahn K, Jo EJ, Choi GJ, Nou I-S, Yu Y, Yang T-J (2015) Genotyping-by-sequencing map permits identification of clubroot resistance QTLs and revision of the reference genome assembly in cabbage (Brassica oleracea L.). DNA Res 23:29-41. Leflon M, Eber F, Letanneur J, Chelysheva L, Coriton O, Huteau V, Ryder C, Barker G, Jenczewski E, Chèvre A (2006) Pairing and recombination at meiosis of Brassica rapa (AA) × Brassica napus (AACC) hybrids. Theor Appl Genet 113:1467-1480. Lei S, Yao X, Yi B, Chen W, Ma C, Tu J, Fu T (2007) Towards map-based cloning: fine mapping of a recessive genic male-sterile gene ( BnMs2 ) in Brassica napus L. and syntenic region identification based on the Arabidopsis thaliana genome sequences. Theor Appl Genet 115:643-651. Levings CS, III (1990) The Texas cytoplasm of maize: cytoplasmic male sterility and disease susceptibility. Science 250:942-947. doi:10.1126/science.250.4983.942 Li CW (1981) The origin, evolution, taxonomy and hybridization of Chinese cabbage. Chinese cabbage, Proceedings of the First International Symposium:3-11. Li SL, Qian YX, Wu ZH (1985) Inheritance of genic male sterility in Brassica napus and its application to commercial production. Acta Agriculturae Shanghai 1:1-12. Li SL, Qian YX, Wu ZH, Stefansson BR (1988) Genetic male sterility in rape (Brassica napus L.) conditioned by interaction of genes at two loci. Canadian Journal of Plant Science 68:1115-1118. Li SL, Zhou ZJ, Zhou XR (1993) Inheritance of recessive genic male sterile line S45AB of rape (Brassica napus L.). Acta Agriculturae Shanghai 9:1-7 Li X, Wang A, Zu F, Hu Z, Lin J, Zhou G, Tu J (2016) Identification of a nuclear-recessive gene locus for male sterility on A2 chromosome using the Brassica 60 K SNP array in non-heading Chinese cabbage. Genes Genomics 38:1151-1157. Liu J, Hong D, Lu W, Liu P, He Q, Yang G (2008) Genetic analysis and molecular mapping of gene associated with dominant genic male sterility in rapeseed (Brassica napus L.). Genes Genomics 30:523-532. Lu GY, Yang GS, Fu TD (2004) Molecular mapping of a dominant genic male sterility gene Ms in rapeseed ( Brassica napus). Plant Breed 123:262-265. Lu W, Liu J, Xin Q, Wan L, Hong D, Yang G (2013) A triallelic genetic male sterility locus in Brassica napus: an integrative strategy for its physical mapping and possible local chromosome evolution around it. Ann Bot 111:305-315. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. PNAS (USA) 88:9828-9832. Mizuno H, Katagiri S, Kanamori H, Mukai Y, Sasaki T, Matsumoto T, Wu J (2020) Evolutionary dynamics and impacts of chromosome regions carrying R-gene clusters in rice. Sci Rep 10:872. doi:10.1038/s41598-020-57729-w Morris GP, Ramu P, Deshpande SP, Hash CT, Shah T, Upadhyaya HD, Riera-Lizarazu O, Brown PJ, Acharya CB, Mitchell SE, Harriman J, Glaubitz JC, Buckler ES, Kresovich S (2013) Population genomic and genome-wide association studies of agroclimatic traits in sorghum. PNAS (USA) 110:453-458. Nagaharu U (1935) Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization Jpn J Bot 7:389-452. Navabi ZK, Parkin IAP, Pires JC, Xiong Z, Thiagarajah MR, Good AG, Rahman MH (2010) Introgression of B-genome chromosomes in a doubled haploid population of Brassica napus × B. carinata. Genome 53:619-629. doi:10.1139/G10-039 Nieuwhof M (1961) Male sterility in some cole crops. Euphytica 10 (3):351-356. Ogura H (1968) Studies on the new male sterility in Japanese radish, with special reference to the utilization of this sterility towards the practical raising of hybrid seeds. Memoirs of the Faculty of Agriculture 6:39-78. Otyama PI, Wilkey A, Kulkarni R, Assefa T, Chu Y, Clevenger J, O’Connor DJ, Wright GC, Dezern SW, MacDonald GE, Anglin NL, Cannon EKS, Ozias-Akins P, Cannon SB (2019) Evaluation of linkage disequilibrium, population structure, and genetic diversity in the U.S. peanut mini core collection. BMC Genomics 20 (1):481. doi:10.1186/s12864-019-5824-9 Pan T, Zeng F, Wu S, Zhao Y (1988) A study on the breeding and application GMS line of low eruci acid in rapeseed (B. napus). Chinese Journal of Oil Crop Sciences 3:5-8. Pelletier G, Primard C, Vedel F, Chetrit P, Remy R, Rousselle, Renard M (1983) Intergeneric cytoplasmic hybridization in cruciferae by protoplast fusion. Molecular and General Genetics 191:244-250. Poland J, Brown P, Sorrells M, Jannink J-L (2012) Development of high-density genetic maps for barley and wheat using a novel two-enzyme genotyping-by-sequencing approach. PLoS ONE 7 (2):e32253. doi: 10.1371/journal.pone.0032253 Polowick PL, Sawhney VK (1988) High Temperature Induced Male and Female Sterility in Canola (Brassica napus L.). Ann Bot 62:83-86. Rajcan I, Kasha KJ, Kott LS, Beversdorf WD (2002) Evaluation of cytoplasmic effects on agronomic and seed quality traits in two doubled haploid populations of Brassica napus L. Euphytica 123:401-409. Ruffio-Chable V, Bellis H, Herve Y (1993) A dominant gene for male sterility in cauliflower (Brassica oleracea var. botrytis): phenotype expression, inheritance, and use in F1 hybrid production. Euphytica 97: 9-17. Sawhney VK (2004) Photoperiod-sensitive male-sterile mutant in tomato and its potential use in hybrid seed production. The Journal of Horticultural Science Biotechnology 79:138-141. Sodhi YS, Chandra A, Verma JK, Arumugam N, Mukhopadhyay A, Gupta V, Pental D, Pradhan AK (2006) A new cytoplasmic male sterility system for hybrid seed production in Indian oilseed mustard Brassica juncea. Theor Appl Genet 114:93-99. Song L-Q, Fu T-D, Tu J-X, Ma C-Z, Yang G-S (2006) Molecular validation of multiple allele inheritance for dominant genic male sterility gene in Brassica napus L. Theor Appl Genet 113:55-62. Spindel JE, Begum H, Akdemir D, Collard B, Redoña E, Jannink JL, McCouch S (2016) Genome-wide prediction models that incorporate de novo GWAS are a powerful new tool for tropical rice improvement. Heredity 116:395-408. Sun Z, Wang Z, Tu J, Zhang J, Yu F, McVetty PBE, Li G (2007) An ultradense genetic recombination map for Brassica napus, consisting of 13551 SRAP markers. Theor Appl Genet 114:1305-1317. Suwabe K, Tsukazaki H, Iketani H, Hatakeyama K, Kondo M, Fujimura M, Nunome T, Fukuoka H, Hirai M, Matsumoto S (2006) Simple Sequence Repeat-Based Comparative Genomics Between Brassica rapa and Arabidopsis thaliana: The Genetic Origin of Clubroot Resistance. Genetics 173:309-319. Takahata Y, Nagasaka M, Kondo H, Kaizuma N (1996) Genic male sterility in Brassica campestris L. Acta Hort 407:147-150. Thompson KF (1972) Cytoplasmic male sterility in oilseed rape. Heredity 29:253-257 Tu J, Fu T, Zheng Y (1997) Analysis on inheritance and isolocus of the rapeseed GMS 90-2441A (B. napus L.). Journal of Huazhong Agricultural University 16:255-258. Van der Meer QP (1987) Chromosomal monogenic dominant male sterility in chinese cabbage (Brassica rapa supbsp. pekinensis (Lour.) hanelt). Euphytica 36:927-931. Van Os H, Stam P, Visser RGF, van Eck HJ (2005) SMOOTH: a statistical method for successful removal of genotyping errors from high-density genetic linkage data. Theor Appl Genet 112:187-194. Verma JK, Sodhi YS, Mukhopadhyay A, Arumugam N, Gupta V, Pental D, Pradhan AK (2000) Identification of stable maintainer and fertility restorer lines for 'polima' CMS in Brassica campestris. Plant Breed 119:90-92. Wan Z, Jing B, Tu J, Ma C, Shen J, Yi B, Wen J, Huang T, Wang X, Fu T (2008) Genetic characterization of a new cytoplasmic male sterility system (hau) in Brassica juncea and its transfer to B. napus. Theor Appl Genet 116 :355-362. Wang X, Lou P, Bonnema G, Yang B, He H, Zhang Y, Fang Z (2005) Linkage mapping of a dominant male sterility gene Ms-cd1 in Brassica oleracea. Genome 48:848-854. Wang Y, Sun S, Liu B, Wang H, Deng J, Liao Y, Wang Q, Cheng F, Wang X, Wu J (2011) A sequence-based genetic linkage map as a reference for Brassica rapa pseudochromosome assembly. BMC Genomics 12 (1):239. doi:10.1186/1471-2164-12-239 Xiao L, Yi B, Chen Y, Huang Z, Chen W, Ma C, Tu J, Fu T (2008) Molecular markers linked to Bn;rf : a recessive epistatic inhibitor gene of recessive genic male sterility in Brassica napus L. Euphytica 164:377-384. Xie Y, Dong F, Hong D, Wan L, Liu P, Yang G (2012) Exploiting comparative mapping among Brassica species to accelerate the physical delimitation of a genic male-sterile locus (BnRf) in Brassica napus. Theor Appl Genet 125:1-12. Xie YZ, Hong DF, Xu ZH, Liu PW, Yang GS (2008) Identification of AFLP markers linked to the epistatic suppressor gene of a recessive genic male sterility in rapeseed and conversion to SCAR markers. Plant Breed 127:145-149. Xu Z, Xie Y, Hong D (2009) Fine mapping of the epistatic suppressor gene (esp) of a recessive genic male sterility in rapeseed (Brassica napus L.). Genome 52:755-760. Yi B, Chen Y, Lei S, Tu J, Fu T (2006) Fine mapping of the recessive genic male-sterile gene (Bnms1) in Brassica napus L. Theor Appl Genet 113:643-650. Ying M, Dreyer F, Cai D, Jung C (2003) Molecular markers for genic male sterility in Chinese cabbage. Euphytica 132:227-234. Zhang H, Zhang S-j, Wu J, Li F, Zhang S-f, Wang X-w, Zhang X-m, Sun R-f (2010) Mapping of recessive genic male sterile restoring gene (BrMf2) in Brassica rapa L. ssp. pekinensis. Scientia Agricultura Sinica 43:993-999. Zhang L-G, Hao D-F (2001) Investigation on the sterility changeover of male sterility Line CMS7311 in heading Chinese cabbage. Acta Botanica Sinica 43:1123-1128. Zhou Y, Tang H, Cheng MP, Dankwa KO, Chen ZX, Li ZY, Gao S, Liu YX, Jiang QT, Lan XJ, Pu ZE, Wei YM, Zheng YL, Hickey LT, Wang JR (2017) Genome-wide association study for pre-harvest sprouting resistance in a large germplasm collection of Chinese wheat landraces. Front Plant Sci 8:401. doi:10.3389/fpls.2017.00401 Zu F, Xia S, Dun X, Zhou Z, Zeng F, Yi B, Wen J, Ma C, Shen J, Tu J, Fu T (2010) Analysis of genetic model of a recessive genic male sterile line 7-7365AB in Brassica napus L. based on molecular markers. Scientia Agricultura Sinica 43:3067-3075. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/8270 | - |
dc.description.abstract | 白菜類作物是重要的蔬菜來源之一,為了應用雄不稔性生產具雜種優勢的商業F1種子,急需豐富雄不稔基因的遺傳資源。本研究在小油菜(Brassica rapa ssp. chinensis var. oleifera Makino)地方品種中發現具雄不稔性的自發性突變體,利用後裔檢定進行系統性的遺傳分析,結果顯示該性狀符合單一基因座上三個複等位基因控制的遺傳模式,將該基因命名為BrMs,而此三個複等位基因間的顯隱性關係為BrMsa > BrMsb > BrMsc,其中控制雄不稔性表現者為BrMsb,此遺傳模式為不結球白菜上首見,透過genotyping-by-sequencing (GBS)策略針對不同雄不稔等位基因建構之F2及BC1分離族群進行基因定位,不同雄不稔等位基因均被定位於Chr07染色體之相同區域而驗證遺傳分析的結論。 依據GBS粗定位之區間標誌(bin markers)設計為單一核苷酸多型性(single nucleotide polymorphism, SNP)分子標誌,並以包含1,590株的F2分離族群進行驗證,其中有二個SNP 分子標誌分別位於雄不稔基因的二側,與基因之遺傳距離各均為0.3 cM而將BrMs限縮於約400 kb範圍內,為比較不同雄不稔基因間之差異,結球白菜雄不稔基因Ms的序列特徵化增幅區域(sequence characterized amplified region, SCAR)分子標誌也成功的轉換為SNP 分子標誌,並分析相同F2族群,連鎖分析顯示Ms基因雖亦屬Chr07連鎖群但與本研究之BrMs基因位於不同區域,然而透過基因體比較分析發現大油菜雄不稔基因BnRf位於本研究BrMs基因粗定位範圍內,進一步依據BnRf之基因序列,分析本研究雄不稔系與恢復系其BnRf 同源基因之差異而開發了一個Indel分子標誌,以該分子標誌偵測前述F2族群之923個植株,結果顯示該分子標誌基因分型的結果與外表型完全一致。 為了進行BrMs基因的精細定位,將雄不稔系NH80-A與恢復系TA95進行全基因體定序,針對BrMs粗定位區間比較二品系DNA序列之差異,並開發了9個SNP分子標誌用以分析4,415株 F2族群,基因精細定位的結果顯示BrA7_6746K與BrA7_6749K等二個SNP分子標誌與雄不稔外表型共分離,然而依據BnRf 而設計的Indel分子標誌進行基因分型時,發現2個體其外表型與分子標誌的基因型不一致,推測該個體可能為複合基因內重組的結果,透過基因精細定位將BrMs限縮於24 kb範圍內,該區域僅包含Bra014987、Bra014988及Bra014989等3個預測基因(putative genes),可做為據圖選殖(map-based cloning)之參考。 正常稔性個體其BrMs基因型大都不具BrMsb等位基因,為了開發可鑑別BrMsa與BrMsc等位基因之分子標誌,利用140個B. rapa自交系與NH80-A進行測交而分析其BrMs基因型,並針對整個族群進行全基因體關連性分析(genome-wide association study, GWAS),結果於Chr07染色體上探勘出17個SNPs與BrMs基因具關聯性,挑選4個設計為SNP分子標誌,其中TqBrA7_6750K基因分型的結果顯示其可有效鑑別BrMsa與BrMsc等位基因。 本研究解析本土小油菜細胞核雄不稔性之遺傳機制,研究結果顯示其遺傳行為具商業應用潛力,並可擴大應用於白菜類作物,BrMs基因定位之結果及開發的分子標誌,除可做為基因選殖之參考外,亦有助於透過分子育種加速選育白菜類作物之雄不稔品系。 | zh_TW |
dc.description.abstract | Consisting of many crop subspecies, Brassica rapa is cultivated as a very important source of vegetables. Despite male sterility has been widely used in commercial production of hybrid seeds in brassica crops, but not in B. rapa due to lack of useful genetic resources with male sterile genes. We discovered a spontaneous mutant from a local variety of rape (B. rapa ssp. chinensis var. oleifera Makino) in Taiwan. Results from a series of progeny tests indicated that male sterility in B. rapa ssp. chinensis fits a one gene with multi-allelic model. The gene designated as BrMs is composed of three alleles with BrMsa dominant to BrMsb, and BrMsb dominant to BrMsc (BrMsa > BrMsb > BrMsc). Among three alleles, BrMsb conferring male sterility and the genotype with BrMsbMsb and BrMsbMsc would be sterile. This is the first report of such genetic model in non-heading Chinese cabbage. The model was confirmed according to the results of gene mapping under the genotyping-by-sequencing (GBS) strategy, which was that the male sterility loci were mapped at the same region of chromosome 07 using BC1 and F2 populations involved different BrMs alleles. SNP markers were designed based on GBS bin markers co-segregating with BrMs trait to evaluate genetic distance between BrMs and markers loci based on 1,590 F2 plants derived from male sterile (NH80-A) and restorer line (TA95). Two flanking SNP markers, BrA7_6632K and BrA7_7625K, were only 0.3 cM away from the upstream and downstream of BrMs locus respectively, narrowing the male sterility gene down to a 400 kb region. A SNP marker, syau_scr04_SNP, developed from sequence characterized amplified region (SCAR) marker closely linked to Ms locus in Chinese cabbage was used to investigate the same F2 population. The result of linkage analysis suggested that BrMs and Ms were located on the same A07 chromosome but at different loci. Interestingly, comparative genomic analysis showed that a male sterile gene in Brassica napus, BnRf, was within the 400 kb region of the BrMs locus. The BnRf orthologs of B. rapa ssp. chinensis were sequenced, and one indel marker (BrMs_indel) was developed for genotyping the same F2 population. Male sterility phenotypes of all 923 investigated plants were completely co-segregated with the marker genotypes. To perform gene fine-mapping for the BrMs, the whole genome sequencing of the male sterile line NH80-A and restorer line TA95 was accomplished. Within the interval detected from primary BrMs gene mapping, the variations of DNA sequences between two lines were developed into 9 SNP markers. These SNP markers and BrMs_indel marker were investigated in 4,415 plants of an F2 population derived from NH80-A × TA95. The genotyping results showed that two SNP markers, BrA7_6746K and BrA7_6749K, were co-segregated with male sterility. Two out of 4,415 plants showed no co-segregation between male sterility phenotypes and BrMs_indel genotypes, and it might be resulted from the intragenic recombination within the BrMs gene complex. In addition, the results of fine mapping had located BrMs to a 24 kb physical region. This area contains only 3 putative genes, Bra014987, Bra014988 and Bra014989, providing solid information for map-based cloning. Despite BrMsb conferring male sterility in B. rapa, most male fertile varieties retain BrMsa or BrMsc but not BrMsb allele in the locus. In order to develop molecular markers to distinguish BrMsa and BrMsc alleles, 140 B. rapa inbred lines were crossed with NH80-A to test their BrMs genotype, and followed by performing genome-wide association studies (GWAS). Seventeen SNPs on the Chr07 chromosome associated with BrMs were found, and four of them were selected to develop SNP markers. Genotyping result of examined B. rapa accessions indicated that TqBrA7_6750K marker could be used to discriminate BrMsa, and BrMsc alleles effectively. This study explored the genetic mechanism of the new genic male sterility mutation in non-heading Chinese cabbage. The mutated gene has commercial application potential and can be extended to the other B. rapa crops. The results of gene fine mapping and marker development, not only have provided a solid foundation for BrMs gene cloning, but also speeded up the development of male sterile lines of B. rapa crops through molecular breeding. | en |
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dc.description.tableofcontents | 中 文 摘 要 i Abstract iii 第1章 總 論 1 1 前言 2 2 前人研究 3 2.1 小油菜之分類地位及其與蕓苔屬物種間之關係 3 2.2 白菜類之起源 5 2.3 台灣小油菜之栽培史 6 2.4 雄不稔性的類型 6 2.5 十字花科作物細胞質雄不稔性的來源與利用 7 2.6 細胞質雄不稔性應用上可能的限制 8 2.7 蕓苔屬作物細胞核雄不稔性的來源與遺傳 10 2.7.1 隱性基因控制之細胞核雄不稔的遺傳與應用 10 2.7.2 單一顯性基因控制之細胞核雄不稔的遺傳與應用 12 2.7.3 細胞核內二對顯性基因所控制之雄不稔性的遺傳與應用 14 2.7.4 細胞核內複等位基因所控制之雄不稔性的遺傳與應用 15 2.7.5 細胞核內多個基因所控制之雄不稔性的遺傳與應用 16 2.8 雄不稔性相關基因的分子標誌與基因定位 17 第2章 不結球白菜雄不稔性的遺傳模式 21 1 前言 22 2 材料與方法 22 2.1 試驗材料 22 2.2 後裔檢定試驗之遺傳分析 24 2.2.1 雄不稔性遺傳模式之假設 24 2.2.2 試驗材料相互雜交建構後裔檢定分析族群 25 2.2.3 後裔檢定試驗之規模及性狀調查 26 2.2.4 利用後裔檢定試驗之結果推導雄不稔性之遺傳模式 27 3 結果 27 3.1 以TA95為恢復系之第一雜交群組後裔檢定試驗 27 3.2 以SG388為恢復系之第二雜交群組後裔檢定試驗 34 4 討論 39 4.1 測交分析之方法 39 4.2 大油菜與大白菜細胞核雄不稔遺傳之比較 40 4.3 遺傳行為對爾後育種之差異 40 第3章 以雄不稔株 × 恢復系之F2族群建立遺傳連鎖圖定位BrMs基因 41 1 前言 42 2 材料與方法 43 2.1 建構基因定位分離族群 43 2.2 GBS(genotyping-by-sequencing)之進行 43 2.2.1 建構GBS library 43 2.2.2 序列分析(raw sequence data processing) 44 2.3 連鎖分析(linkage analysis) 44 3 結果 44 3.1 GBS的結果 44 3.2 BrMs之基因定位(gene mapping) 45 4 討論 53 4.1 透過GBS策略建構連鎖圖以進行基因定位 53 4.2 本研究遺傳連鎖圖與其他不結球白菜連鎖圖之比較 54 第4章 以雄不稔株 × 維持系之BC1族群建立遺傳連鎖圖定位BrMs基因 55 1 前言 56 2 材料與方法 56 2.1 建構基因定位分離族群 56 2.2 GBS之進行 56 2.2.1 建構GBS library 56 2.2.2 序列分析 57 2.2.3 連鎖分析 57 3 結果 58 3.1 GBS的結果 58 3.2 BrMs之基因定位 58 4 討論 69 4.1 BC1遺傳連鎖圖與F2連鎖圖之比較 69 4.2 基因定位結果驗證BrMsa、BrMsb及BrMsc的等位性 69 第5章 BrMs基因之精細定位及開發其SNP分子標誌 71 1 前言 72 2 材料與方法 73 2.1 粗定位之GBS bin markers 驗證 73 2.1.1 建構BrMs基因之分離族群 73 2.1.2 將GBS及SCARs markers轉換為SNP 分子標誌 73 2.1.3 參試植株基因分型及BrMs連鎖分析 74 2.2 不結球白菜BrMs與大油菜BnRf之關係 74 2.2.1 不結球白菜BnRf orthologs間序列比較及引子設計 74 2.2.2 BnRf orthologs 之DNA片段選殖與定序 75 2.2.3 BrMs與BnRf ortholog之等位性分析 76 2.3 BrMs基因之精細定位 76 2.3.1 基因體定序及序列分析 76 2.3.2 開發精細定位用SNP分子標誌 77 2.3.3 精細定位用分離族群之建構與基因分型 77 3 結果與討論 78 3.1 粗定位之GBS markers的驗證 78 3.1.1 將GBS markers轉換為SNP分子標誌 78 3.1.2 結球白菜Ms基因之SCAR分子標誌轉換為SNP分子標誌 78 3.1.3 SNP分子標誌與BrMs基因之連鎖分析結果 82 3.1.4 不結球白菜雄不稔BrMs基因與結球白菜Ms基因間可能之關係 83 3.1.5 SNP分子標誌開發策略及應用於MAS之潛力 83 3.1.6 近緣種大油菜之BnRf基因位於不結球白菜BrMs之定位區間 84 3.2 不結球白菜BrMs與大油菜BnRf之關係 84 3.2.1 不結球白菜BnRf orthologs間序列之選殖與比較 84 3.2.2 不結球白菜BrMs與油菜BnRf之等位性 87 3.3 BrMs基因之精細定位 87 3.3.1 不結球白菜基因體定序與組裝結果 87 3.3.2 開發精細定位用SNP 分子標誌 89 3.3.3 精細定位BrMs基因 91 第6章 利用全基因體關連分析(GWAS)開發鑑別BrMsa/BrMsc等位基因之SNP分子標誌 95 1 前言 96 2 材料與方法 97 2.1 試驗材料 97 2.2 GBS之進行 98 2.2.1 建構GBS library 98 2.2.2 序列分析 99 2.3 全基因體關聯性分析(GWAS) 100 2.4 SNP分子標誌之設計與驗證 100 3 結果與討論 101 3.1 次世代定序所獲得之讀序 101 3.2 透過GBS探勘獲得之SNPs 103 3.3 GWAS分析之族群組成 107 3.4 GWAS分析的結果 113 3.5 SNP分子標誌的驗證 116 第7章 總結 125 參考文獻 131 附 錄 141 附錄 1 兩種遺傳模式下不結球白菜參試品系可能的基因型 141 附錄 2 Progeny test A之各種雜交組合後裔雄不稔性狀分離情形 142 附錄 3 Progeny test B之各種雜交組合後裔雄不稔性狀分離情形 143 附錄 4 Progeny test C之各種雜交組合後裔雄不稔性狀分離情形 144 附錄 5 本研究部份內容所發表之報告 145 | |
dc.language.iso | zh-TW | |
dc.title | 不結球白菜雄不稔性之遺傳分析、基因精細定位及基因體關聯性之研究 | zh_TW |
dc.title | Genetic Analysis, Gene Fine-mapping, and Genome-wide Association Studies on Male Sterility in Brassica rapa ssp. chinensis | en |
dc.type | Thesis | |
dc.date.schoolyear | 108-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 曾富生(Fu-Shen Thseng),羅筱鳳(Hsiao-Feng Lo),王仕賢(Dennis Wang),林學詩(Hsueh-Shih Lin),謝明憲(Ming-Hsien Hsieh) | |
dc.subject.keyword | 不結球白菜,雄不稔性,遺傳分析,遺傳連鎖圖譜,基因定位,單一核苷酸多型性分子標誌,育種, | zh_TW |
dc.subject.keyword | non-heading Chinese cabbage,male sterility,genetic analysis,genetic map,gene mapping,SNP marker,plant breeding, | en |
dc.relation.page | 155 | |
dc.identifier.doi | 10.6342/NTU202002822 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2020-08-13 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農藝學研究所 | zh_TW |
dc.date.embargo-lift | 2023-08-11 | - |
顯示於系所單位: | 農藝學系 |
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