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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林雅芬(Ya-Fen Lin) | |
| dc.contributor.author | Pei-Chu Liao | en |
| dc.contributor.author | 廖珮筑 | zh_TW |
| dc.date.accessioned | 2022-11-25T07:45:23Z | - |
| dc.date.available | 2023-09-01 | |
| dc.date.copyright | 2021-11-02 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-09-22 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/82468 | - |
| dc.description.abstract | "新興汙染元素鎵 (Gallium, Ga) 和銦 (Indium,In) 是製造高科技產品的重要原料，近年來大量使用，造成其對環境汙染及糧食安全之疑慮；然而鎵或銦如何影響作物生長之機制，目前尚未明瞭。本實驗以水稻 (Oryza sativa L. cv. Taikeng 9) 為材料，研究水稻在鎵 (GaCl3) 或銦處理 (InCl3) 下的生長，並進一步分析水稻在銦處理下生理和分子層次之變化。結果顯示，鎵處理不影響水稻生長，而銦處理明顯抑制水稻生長，使根長縮短、植株矮化、生物量減少、葉片出現壞死斑點，並改變根部細胞構造，如：表皮細胞變大及厚壁層細胞壁變薄。銦主要累積在根部，僅少部分轉運至地上部；銦處理亦會影響必需營養元素累積，導致根部磷、鎂、鐵元素濃度的降低。全轉錄組定序 (RNA sequencing) 分析結果證實，銦處理會誘導蛋白質磷酸化、防禦反應及元素轉運相關基因的表現，以應對銦逆境對水稻的傷害；同時也會觸發水稻缺磷反應、蛋白質降解和細胞凋亡相關基因表現，造成銦毒害水稻之性狀。雖然銦處理促使水稻發生缺磷之反應，但透過比較水稻在銦和缺磷處理下的形態變化，我們發現銦 (而非缺磷) 才是讓水稻呈現毒害性狀的主要因素。有趣的是，施加磷酸可以緩解水稻的銦毒害，暗示其在農業上的可能應用。磷肥用於減緩新興污染元素銦毒害之相關研究，值得進一步探討。" | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-25T07:45:23Z (GMT). No. of bitstreams: 1 U0001-2209202116174000.pdf: 12564060 bytes, checksum: 83c22d2e9d301e02c532ed7bdc86ed58 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | "口試委員會審定書 I 誌謝 II 中文摘要 III 英文摘要 IV 目錄 V 表目錄 X 圖目錄 XI 附錄表目錄 XV 縮寫表 1 前言 2 1. 新興汙染元素 2 2. 銦 2 2.1 銦的特性和用途 2 2.2 銦的生產和汙染 3 2.3 銦的化合物 4 3. 銦的毒害 4 3.1 銦對動物和人類的影響 4 3.2 銦對植物的影響 5 4. 銦於台灣之現況 5 5. 轉錄體分析 6 6. 研究動機和目的 6 材料與方法 8 1. 植物材料 8 1.1 材料 8 1.2 種植環境 8 1.3 水耕栽培 8 1.4 種子消毒和催芽 8 1.5 水稻育苗 9 2. 試驗處理 9 2.1 不同濃度鎵處理 9 2.2 不同濃度銦處理 9 2.3 不同天數銦處理 10 2.4 不同程度缺磷和銦處理 10 3. 組織顯微構造觀察 11 3.1 樣品製備 11 3.2 雷射掃瞄共軛焦顯微鏡觀察 11 4. 細胞顯微構造觀察 12 4.1 樣品切片製備 12 4.1.1 包埋 12 4.1.2 切片 12 4.2 顯微鏡觀察 13 5. 活性含氧物染色分析 13 5.1 DAB (3,3‘-diaminobenzidine) 染色法 13 5.2 NBT (Nitro blue tetrazolium) 染色法 14 6. 樣品均質化 14 7. 葉綠素含量測定 15 7.1 葉片褪色 15 7.2 相對葉綠素含量 15 7.3 葉綠素含量 15 8. 丙二醛含量測定 16 9. 抗氧化酵素活性測定 16 9.1 Soluble / Total peroxidase (POD) 16 9.1.1 Soluble POD 16 9.1.2 Total POD 17 9.2 Ascorbate peroxidase (APX)、Catalase (CAT)、Glutathione reductase (GR) 17 9.2.1 樣品酵素液萃取 17 9.2.2 蛋白質定量 18 9.2.3 APX活性測定 18 9.2.4 CAT活性測定 19 9.2.5 GR活性測定 19 10. 植體元素濃度測定 19 10.1 樣品準備 19 10.2 微波消化 20 10.3 感應耦合電漿質譜儀 (Inductively coupled plasma mass spectrometry, ICP-MS) 21 10.4 感應耦合電漿光學發射光譜儀 (Inductively coupled plasma optics emission spectrometer, ICP-OES) 21 11. 水耕液正磷酸鹽濃度測定 22 12. RNA定序 (RNA sequencing, RNA-seq) 23 12.1 RNA萃取 23 12.2 RNA定序 24 12.3 生物資訊分析 25 13. Real-time PCR (RT-qPCR) 26 13.1 RNA萃取 26 13.2 cDNA合成 26 13.3 Primer設計 27 13.4 RT-qPCR 27 14. 統計分析 28 結果 29 1. 新興污染元素對水稻生長之影響 29 1.1 鎵 29 1.1.1 生理性狀 29 1.1.2 氧化逆境反應 29 1.2 銦 31 1.2.1 生理性狀 31 1.2.2 氧化逆境反應 32 1.2.3 組織顯微結構變化 33 2. 銦抑制水稻生長 34 2.1 生理性狀 34 2.2 氧化逆境反應 35 2.3 植體元素濃度 35 3. 銦處理水稻轉錄體分析 37 3.1 定序品質和主成分分析 37 3.2 差異性表現基因分析 38 3.3 GO分析 38 3.4 受體激酶 39 3.5 轉錄因子 40 3.6 轉運蛋白和金屬伴護蛋白 41 3.7 泛素-蛋白酶體 43 4. 水稻銦毒害和缺磷之生理反應 43 4.1 生理性狀 44 4.2 組織顯微結構變化 45 4.3 植體元素濃度 45 5. 添加磷緩解銦元素對水稻之毒害 47 5.1 生理性狀 47 5.2 組織顯微結構變化 48 5.3 植體元素濃度 48 5.4 水耕液磷酸鹽濃度 50 討論 51 1. 鎵對水稻生長的影響 51 2. 銦對水稻的毒害 52 3. 水稻對銦的防禦反應 54 4. 銦處理誘導水稻缺磷反應 56 5. 銦與磷之交互作用 58 6. 磷元素緩解水稻的銦毒害 59 結論 61 表 62 圖 67 附錄表 151 參考文獻 190 " | |
| dc.language.iso | zh-TW | |
| dc.title | 新興污染元素銦抑制水稻生長之機制 | zh_TW |
| dc.title | The mechanism of rice growth inhibition by indium – an emerging contamination element | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 葉國禎(Hsin-Tsai Liu),洪傳揚(Chih-Yang Tseng),楊淑怡,羅靜琪 | |
| dc.subject.keyword | 新興汙染元素,銦,水稻,RNA定序,缺磷, | zh_TW |
| dc.subject.keyword | Emerging contaminants,Indium,Oryza sativa L.,RNA sequencing,Phosphorous deficiency, | en |
| dc.relation.page | 200 | |
| dc.identifier.doi | 10.6342/NTU202103288 | |
| dc.rights.note | 同意授權(限校園內公開) | |
| dc.date.accepted | 2021-09-24 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農藝學研究所 | zh_TW |
| dc.date.embargo-lift | 2023-09-01 | - |
| 顯示於系所單位： | 農藝學系 | |
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