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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 許輔(Fuu Sheu) | |
| dc.contributor.author | Jhih-Ting Hsiao | en |
| dc.contributor.author | 蕭智庭 | zh_TW |
| dc.date.accessioned | 2022-11-25T06:33:33Z | - |
| dc.date.copyright | 2021-08-18 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-07-14 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/82199 | - |
| dc.description.abstract | "青花菜 (Brassica oleracea var. italica) 採收後於20℃儲放2至3日即老化並失去商品價值。首先分析青花菜之蛋白質體，發現RNA結合蛋白GR-RBP2 (glycine-rich RNA binding-protein 2) 可能與青花菜採後老化相關，此外，除了GR-RBP2外，冷休克結構域蛋白 (cold shock domain containing-protein，CSP) 也同為富含甘胺酸之蛋白質 (glycine-rich protein)，兩者於阿拉伯芥被證實具有RNA伴護蛋白 (RNA chaperone) 活性且其表現皆受低溫所誘導，因此該兩種蛋白質可能皆參與青花菜老化過程之生理反應。另一方面在採後處理技術中，冷休克處理 (cold shock treatment，CST) 被發現能夠延緩部分蔬果老化，故假設CST造成之低溫能誘導此類蛋白質產生，以穩定mRNA結構及轉譯進而延緩採後老化。本研究欲分析青花菜CSP及GR-RBP2與老化之關聯性，並分析其表現是否受CST所誘導，以說明CST誘導CSP及GR-RBP2表現而延緩採後老化之可能性。利用次世代定序建立青花菜轉錄體資料庫，其中45,051個特定基因 (unigenes) 分別有6個註解到CSP基因 (BoCSPs)，2個註解到GR-RBP2 (BoGR-RBP2s)。將青花菜小花貯藏於20℃下3天、5℃及0℃下15天，以青花菜花苞葉綠素損失率作為老化指標，分析其與上述8個基因表現量之相關性。結果顯示BoCSP1_1、BoCSP3、BoCSP4_1、BoGR-RBP2_1及BoGR-RBP2_2與老化呈現負相關。將青花菜小花浸泡0℃冰水浴30分鐘進行CST後放置於5℃貯藏，並於第0、16、24、40及48小時收樣，分析BoCSPs與BoGR-RBP2s之表現量是否受CST所誘導，結果顯示BoCSP2、BoCSP3、BoCSP4_1及BoCSP4_2表現量可受CST所提升。前述兩項實驗結果顯示，BoCSP3與BoCSP4_1與老化呈負相關，且其表現量受CST所提升，說明冷休克處理可能經由誘導CSP之產生，得以延緩青花菜老化。未來可深入探討其受CST調控之訊息傳遞路徑及其於採後生理之作用機制。" | zh_TW |
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| dc.description.tableofcontents | 誌謝 I 摘要 II ABSTRACT III CONTENTS V LIST OF TABLES VII LIST OF FIGURES VIII CHAPTER 1 INTRODUCTION 1 1.1 Cold shock domain-containing proteins in prokaryotes 1 1.2 Cold shock domain-containing protein in eukaryotes 2 1.2.1 UNR protein and Rrp44 protein 3 1.2.2 Y-box protein family 3 1.2.3 Lin28 protein family 4 1.3 Cold shock domain-containing protein in plant kingdom 4 1.3.1 Cold shock domain-containing protein in Arabidopsis thaliana 5 1.3.2 Cold shock domain-containing protein in wheat 6 1.3.3 Cold shock domain-containing protein in Oryza sativa 6 1.4 Glycine-rich proteins with RNA recognition motif in plants 7 1.5 Cold shock treatment in postharvest 9 1.6 Introduction of broccoli (Brassica oleracea var. italica) 10 1.7 Postharvest senescence of broccoli 12 1.8 Aim of this study 13 CHAPTER 2 MATERIALS AND METHODS 15 2.1 Plant materials and treatments 15 2.2 Total RNA purification for transcriptome analysis and qPCR 15 2.3 RNA sequencing and assembly of broccoli transcriptome 16 2.4 Functional annotation of de novo assembled contigs 16 2.5 Determination of total chlorophyll loss in flower buds 17 2.6 Gene expression analysis using qPCR 17 2.7 Statistical analysis and correlation analysis 18 CHAPTER 3 RESULTS 19 3.1 Preliminary proteomic analysis of fresh and senesced broccolis 19 3.2 Sequencing and assembly of B. oleracea var. italica transcriptome 20 3.3 Annotation of unigenes to explore BoCSPs and BoGR-RBP2s 20 3.4 Correlation analysis among chlorophyll loss and gene expression of BoCSPs and BoGR-RBP2s 22 3.5 Relative gene expression of BoCSPs and BoGR-RBP2s after CST 24 CHAPTER 4 DISCUSSION 27 4.1 BoCSPs and BoGR-RBP2s expression during florets senescence 27 4.1.1 Negative correlation among five genes and floret senescence 27 4.1.2 Probable cold-regulated BoCSPs and BoGR-RBP2s 28 4.1.3 Non-senescence-related BoCSPs 29 4.2 Response of BoCSPs and BoGR-RBP2s to CST 29 4.2.1 Expression of four CST-induced genes 29 4.2.2 Four non-CST -inducible genes 30 4.3 Regulation and functions of CSPs in postharvest senescence and CST 32 4.4 Conclusion 33 REFERENCE 34 TABLES 45 FIGURES 51 APPENDIX 62 | |
| dc.language.iso | en | |
| dc.subject | 富含甘胺酸之蛋白質 | zh_TW |
| dc.subject | RNA伴護蛋白 | zh_TW |
| dc.subject | 冷休克處理 | zh_TW |
| dc.subject | 冷休克結構域 | zh_TW |
| dc.subject | RNA辨識超二級結構 | zh_TW |
| dc.subject | Cold shock treatment | en |
| dc.subject | RNA recognition motif | en |
| dc.subject | Glycine-rich protein | en |
| dc.subject | Cold shock domain | en |
| dc.subject | RNA chaperone | en |
| dc.title | 探討青花菜冷休克蛋白CSPs與RNA結合蛋白GR-RBP2基因及其表現 | zh_TW |
| dc.title | Exploration of Cold Shock Domain-Containing Proteins and Glycine-Rich RNA Binding Protein 2 Genes in Brassica oleracea var. italica and Investigation of Their Expressions | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 蘇南維(Hsin-Tsai Liu),周志輝(Chih-Yang Tseng),官彥州 | |
| dc.subject.keyword | RNA辨識超二級結構,富含甘胺酸之蛋白質,冷休克結構域,RNA伴護蛋白,冷休克處理, | zh_TW |
| dc.subject.keyword | RNA recognition motif,Glycine-rich protein,Cold shock domain,RNA chaperone,Cold shock treatment, | en |
| dc.relation.page | 73 | |
| dc.identifier.doi | 10.6342/NTU202101405 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2021-07-15 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 園藝暨景觀學系 | zh_TW |
| dc.date.embargo-lift | 2024-07-01 | - |
| 顯示於系所單位： | 園藝暨景觀學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| U0001-1207202114494200.pdf 未授權公開取用 | 4.05 MB | Adobe PDF |
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