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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳光超(Guang-Chao Chen) | |
| dc.contributor.author | Shih-Yuan Chen | en |
| dc.contributor.author | 陳世原 | zh_TW |
| dc.date.accessioned | 2022-11-25T05:36:20Z | - |
| dc.date.available | 2026-10-18 | |
| dc.date.copyright | 2021-11-02 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-10-19 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/82123 | - |
| dc.description.abstract | "ZER1蛋白屬於ZYG11家族(ZYG11A, ZYG11B和ZER1)的其中之一，它們是Cullin2-RING泛素連接酶複合物(CRL2)的受體辨識亞單位。目前，ZER1在哺乳動物細胞中的生理作用還不太清楚。而ZER1的果蠅直系同源物，稱為dZER1。根據我們之前的實驗知道RagC-D會和dZER1有交互作用。Rag蛋白是鳥嘌呤核苷酸結合蛋白，負責調節 TOR的空間募集和活化，進而調控細胞自噬。因此，我們進一步研究ZER1在細胞自噬和mTOR信號傳導中的功能，同時，我們還透過 TurboID 鄰近標記-質譜分析去探索ZER1 相互作用組。 然而，在哺乳動物細胞中敲落ZER1並未發現對細胞自噬或mTOR途徑有任何顯著影響。而我們的研究顯示不僅是ZER1，ZYG11A和ZYG11B也會與 Rag蛋白相互作用，指向ZYG11家族具有功能重疊。另一方面，根據質譜數據的富集分析，我們發現ZER1與蛋白質折疊、核糖核酸調控和細胞核轉運有關。此外我們也找到一些與細胞自噬相關的潛在相互作用物，並在共免疫沉澱法測定後確實與ZER1相互作用。總結上述發現，我們未來將關注整個ZYG11家族如何在細胞自噬和 mTOR 信號傳導中作用，並且我們也會確定哪些ZER1 潛在相互作用組確實是ZER1的受體以提供未來的研究。 " | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-25T05:36:20Z (GMT). No. of bitstreams: 1 U0001-1510202100273100.pdf: 3903781 bytes, checksum: af7036342cb0c883099947c213de900a (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | "中文摘要 I Abstract II Introduction 6 1. Autophagy 6 2. mTOR signaling pathway 9 3. Proximity labeling 13 4. Cullin2-RING E3 ubiquitin ligase complexes 14 Material and methods 19 Results 28 1. ZER1 may be associated with mTOR signaling and autophagy 28 2. Knockdown of ZER1 does not affect autophagy and mTOR signaling 29 3. ZYG11A and ZYG11B may have overlapping functions with ZER1 30 4. TurboID based proximity labeling is applied in the study of the ZER1 interactome 32 5. The major role of ZER1 is associated with protein folding, nuclear transport, and DNA RNA regulation 33 6. Identification of ZER1 interactors 34 Discussion 36 Reference 40 Figures 52" | |
| dc.language.iso | en | |
| dc.subject | ZYG11家族 | zh_TW |
| dc.subject | TurboID | zh_TW |
| dc.subject | 細胞自噬 | zh_TW |
| dc.subject | mTOR信號傳導 | zh_TW |
| dc.subject | ZER1 | zh_TW |
| dc.subject | autophagy | en |
| dc.subject | mTOR signaling | en |
| dc.subject | ZER1 | en |
| dc.subject | ZYG11 family | en |
| dc.subject | TurboID | en |
| dc.title | 探索 ZER1蛋白在哺乳細胞中之分子功能 | zh_TW |
| dc.title | Exploring the Molecular Function of ZER1 in Mammalian Cells | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 楊維元(Hsin-Tsai Liu),顏雪琪(Chih-Yang Tseng) | |
| dc.subject.keyword | ZER1,ZYG11家族,TurboID,細胞自噬,mTOR信號傳導, | zh_TW |
| dc.subject.keyword | ZER1,ZYG11 family,TurboID,autophagy,mTOR signaling, | en |
| dc.relation.page | 70 | |
| dc.identifier.doi | 10.6342/NTU202103740 | |
| dc.rights.note | 同意授權(限校園內公開) | |
| dc.date.accepted | 2021-10-20 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| dc.date.embargo-lift | 2026-10-18 | - |
| 顯示於系所單位： | 生化科學研究所 | |
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