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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳昭瑩(Chao-Ying Chen) | |
| dc.contributor.author | Quan Fong | en |
| dc.contributor.author | 馮全 | zh_TW |
| dc.date.accessioned | 2022-11-25T05:35:08Z | - |
| dc.date.available | 2024-01-01 | |
| dc.date.copyright | 2022-02-17 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2022-01-03 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/82068 | - |
| dc.description.abstract | 為了減少化學農藥的使用與風險，需要開發化學農藥的替代資材，微生物農藥即為其中之一。多黏類芽孢桿菌(Paenibacillus polymyxa)菌株TP3分離自田間草莓植株的地上部組織，先前的研究顯示其施用於草莓植株具有對灰黴病與炭疽病之防治功效。多黏類芽孢桿菌也會產生具有環境耐受性以及倉儲壽命長的內生孢子，適合選用開發為微生物農藥。本研究以黃豆粉培養基替代AK產孢培養基可產生十倍量的內生孢子，使生產成本降低、提升應用可行性；當於黃豆粉培養基添加米糠、海草粉等碳素源，可增加TP3內生孢子產量，為大量生產TP3之培養基配方的參考依據。透過對峙培養以及孢子發芽抑制試驗，得知TP3具對草莓葉枯病之防治潛力，盆栽試驗證實TP3對草莓葉枯病具有防治效果，並在TP3施用十天內維持防治效果。多項推薦使用於草莓與番茄之化學殺菌劑與殺蟲劑不會抑制TP3的生長，如將TP3與其可耐受之殺菌劑共同施用時，可提升對葉枯病的防治效果；將TP3延伸應用至番茄灰黴病的防治，試驗得知TP3對番茄灰黴病菌之菌絲生長與孢子發芽均具有抑制能力，由盆栽試驗及葉表菌數調查得知TP3施用十天內具有病害防治效果，但TP3菌數會逐漸下降。由於菌株TP3處理可增加番茄葉片由激發子flg22誘導之癒傷葡聚醣沈積，推測誘導抗性(induced resistance)是TP3防治番茄病害的機制之一。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-25T05:35:08Z (GMT). No. of bitstreams: 1 U0001-3012202116245800.pdf: 3411153 bytes, checksum: cc4fdc6284cfb0b42d057b99b33ddbe9 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 中文摘要 i 英文摘要 ii 壹、前言 1 貳、前人研究 2 一、臺灣草莓產業 2 二、草莓葉枯病菌 3 三、臺灣之番茄產業 4 四、番茄灰黴病 5 五、微生物農藥 6 六、多黏類芽孢桿菌 7 七、多黏類芽孢桿菌於植物病害防治之應用 8 八、多黏類芽孢桿菌TP3 9 九、多黏類芽孢桿菌的產孢培養條件 10 參、材料與方法 12 一、 供試植物的栽培 12 1. 草莓植株之培養條件 12 2. 番茄植株之培養條件 12 二、多黏類芽孢桿菌之培養與保存 12 1. TP3之培養與保存 12 3. 發酵生產高濃度之TP3內生孢子濃縮液 13 三、供試菌株之培養 14 1. 草莓葉枯病菌 14 2. 番茄灰黴病菌 14 四、TP3內生孢子生產優化試驗 14 1. 培養溫度對TP3產生內生孢子之影響 14 2. 添加碳素源對TP3內生孢子數量及其對病原菌拮抗能力之影響 15 五、病原菌對峙試驗 15 六、真菌孢子發芽抑制試驗 15 七、TP3於草莓葉表原位防治葉枯病試驗 16 八、盆栽防治試驗 16 1. 連續施用TP3對草莓葉枯病之防治效果 16 2. 施用TP3防治草莓葉枯病之效期試驗 16 3.連續施用TP3對番茄灰黴病菌之防治效果 17 4. 施用TP3防治番茄灰黴病之效期試驗 17 九、TP3之化學農藥耐受性試驗 17 1 . 以濾紙圓盤法(paper disc method)測試TP3對於化學農藥之耐受性 17 2. TP3與農藥共同施用防治草莓葉枯病試驗 17 十、番茄葉表之TP3菌數調查 18 十一、以組織化學染色法檢測番茄防禦反應 18 十二、田間驗證試驗 19 1. TP3之田間施用方法 19 2. TP3施用於田間草莓植株對果實之葉枯病菌感染影響試驗 19 3. TP3施用於田間草莓植株對花及幼果數量影響試驗 19 十三、 統計分析方法 19 肆、結果 21 一、黃豆粉培養基適於生產TP3內生孢子 21 二、TP3培養條件與不正常形態菌落的產生 21 三、多黏類芽孢桿菌TP3可抑制草莓葉枯病菌之菌絲生長與孢子發芽 22 三、以黃豆粉培養基培養之TP3內生孢子培養液可用於防治草莓葉枯病，且具有10天的持效性 22 四、菌株TP3具有對多項化學殺菌劑與殺蟲劑之耐受性，且共同施用可提昇對草莓葉枯病的防治功效 22 五、TP3田間施用可降低草莓果實受葉枯病菌之感染及增加花及幼果數量 23 六、由對峙培養及孢子發芽試驗得知TP3具有抑制番茄灰黴病菌菌絲生長及孢子發芽之能力 23 七、以黃豆粉培養基培養之TP3處理番茄葉片，對灰黴病有至少十天之保護效期，並且可留存於葉表 24 伍、討論 25 草莓葉枯病之生物防治 25 田間試驗 27 陸、參考文獻 32 柒、圖表集 46 表一、多黏類芽孢桿菌TP3與各項化學殺蟲劑、殺菌劑之耐受性 47 圖一、比較黃豆粉培養基與AK產孢培養基之TP3內生孢子產量 48 圖二、以2%黃豆粉培養基培養TP3之內生孢子產量 49 圖三、多黏類芽孢桿菌TP3內生孢子生產之優化 50 圖四、多黏類芽孢桿菌TP3以不同比例之粗原料進行培養之內生孢子產量 51 圖五、比較常溫與冷藏半年之由黃豆粉培養基所培養之TP3內生孢子數量變化 52 圖六、多黏類芽孢桿菌TP3於黃豆粉培養基上之不正常形態菌落及其拮抗能力差異 54 圖七、多黏類芽孢桿菌TP3與草莓葉枯病菌對峙培養 55 圖八、多黏類芽孢桿菌TP3之菌體抑制草莓葉枯病菌之孢子發芽 56 圖九、多黏類芽孢桿菌TP3培養上清液抑制草莓葉枯病菌之孢子發芽 57 圖十、多黏類芽孢桿菌TP3於草莓葉表原位抑制葉枯病之病徵發展 58 圖十一、 黃豆粉培養基培養之多黏類芽孢桿菌TP3抑制草莓葉枯病之效果 59 圖十二、於接種前不同天數處理多黏類芽孢桿菌TP3對草莓葉枯病之防治效果 60 圖十三、多黏類芽孢桿菌TP3與化學殺菌劑分別及共同使用對草莓葉枯病之防治效果 61 圖十四、田間施用多黏類芽孢桿菌TP3對草莓果實之病害防治效果 62 圖十五、田間施用多黏類芽孢桿菌TP3對草莓花及幼果數量之影響 63 圖十六、多黏類芽孢桿菌TP3抑制番茄灰黴病菌之菌絲生長 64 圖十七、多黏類芽孢桿菌TP3營養細胞抑制番茄灰黴病菌孢子發芽情形 65 圖十八、多黏類芽孢桿菌TP3抑制番茄灰黴病之效果 66 圖十九、於接種前不同天數處理多黏類芽孢桿菌TP3對番茄灰黴病之防治效果 67 圖二十、多黏類芽孢桿菌TP3 噴灑後不同天數留存於番茄葉表之菌量 68 圖二十一、多黏類芽孢桿菌TP3促進番茄葉片由flg22誘導之癒傷葡聚醣沉積 69 捌、附錄 70 附圖一、於相位差顯微鏡觀察TP3內生孢子培養液 71 | |
| dc.language.iso | zh-TW | |
| dc.subject | 番茄灰黴病 | zh_TW |
| dc.subject | 多黏類芽桿孢菌 | zh_TW |
| dc.subject | 微生物農藥 | zh_TW |
| dc.subject | 草莓葉枯病 | zh_TW |
| dc.subject | Paenibacillus polymyxa | en |
| dc.subject | tomato gray mold | en |
| dc.subject | strawberry leaf blight | en |
| dc.subject | microbial pesticide | en |
| dc.title | 多黏類芽孢桿菌TP3之內生孢子生產及植物病害防治應用 | zh_TW |
| dc.title | Endospore production of Paenibacillus polymyxa TP3 and application in plant disease control | en |
| dc.date.schoolyear | 110-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 洪挺軒(Hsin-Tsai Liu),鍾嘉綾(Chih-Yang Tseng),林乃君,黃健瑞 | |
| dc.subject.keyword | 多黏類芽桿孢菌,微生物農藥,草莓葉枯病,番茄灰黴病, | zh_TW |
| dc.subject.keyword | Paenibacillus polymyxa,microbial pesticide,strawberry leaf blight,tomato gray mold, | en |
| dc.relation.page | 71 | |
| dc.identifier.doi | 10.6342/NTU202104600 | |
| dc.rights.note | 同意授權(限校園內公開) | |
| dc.date.accepted | 2022-01-04 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 植物醫學碩士學位學程 | zh_TW |
| dc.date.embargo-lift | 2024-01-01 | - |
| 顯示於系所單位： | 植物醫學碩士學位學程 | |
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