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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 羅世強(Shyh-Chyang Luo) | |
| dc.contributor.author | Ke-Hsin Wang | en |
| dc.contributor.author | 王可昕 | zh_TW |
| dc.date.accessioned | 2022-11-25T03:03:56Z | - |
| dc.date.available | 2023-08-08 | |
| dc.date.copyright | 2021-11-09 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-08-12 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/81802 | - |
| dc.description.abstract | " 含有聚胜肽之雙親性嵌段共聚高分子因其良好的生物相容性與生物降解性，被視為運用在藥物輸送或組織工程學上富有潛力的材料。尤其因為此種高分子在水中有可形成多種穩定的自組裝結構，更有助於開發精準藥物釋放系統或用做組織工程材料上。然而，含有聚胜肽之雙親性嵌段共聚高分子具有結構上的多變性，同時也使其結構難以預測，這是由於多種作用力參與了自組裝結構的形成。因此，我們以釐清主導結構形成的作用力與影響因素，以及理解自組裝造成凝膠化的機制為研究目標，並整理出能使此種高分子凝膠化的配方，以便未來應用於生醫組織工程中。更具體而言，此篇研究以開發用於治療神經相關疾病的生醫材料為目標，利用聚胜肽聚谷氨酸苯酯（PBG）、聚賴氨酸（PLL）與聚乙二醇（PEG）組成新穎嵌段共聚物並探討其特性。透過精準多肽聚合的技術，我們發現當嵌段共聚物的各鏈段以不同方式排列，會展現出明顯不同的組裝結構。 其中，(Bx-r-Ky)-E34-(Bx-r-Ky)因兩端無規排列的鏈段而組成鬆散的聚集；而對於Ky- Bx-E34-Bx-Ky高分子，當PBG鏈段聚集時PLL鏈段僅能構成開環形結構，使得不同微胞之間因高分子鏈糾纏而產生的作用力減弱，疏水作用力因此成為誘發聚集的主導作用力；另一方面，Bx-Ky-E34-Ky-Bx高分子則因為微胞之間較強的作用力，在水溶液中自組裝成網狀結構，故可在低臨界濃度下形成水凝膠。我們也發現以更長鏈段的PLL取代原有結構中的PEG之後，該高分子有更顯著的凝膠化趨勢。考慮聚胜肽之雙親性嵌段共聚高分子的分子量效應與親/疏水平衡，我們構建了Bx-Ky-E34-Ky-Bx高分子的相圖。該圖顯示了在給定聚合物之組成時，能使高分子在水溶液中構成網狀水凝膠結構的最低濃度；若再結合論文中高分子組成對機械性質的圖表，便能夠快速進一步找到具有所需機械性質的水凝膠製備所需之成分。此研究結果整合高分子成分濃度對於自組裝形態與機械性質的影響，希望使該高分子可做為一種方便使用的體內支架, 促進並修復神經元的生長。 藉由系統性的研究，對此聚胜肽之雙親性嵌段共聚高分子在水溶液中的行為獲得全面性的了解，其中有關嵌段序列強烈影響自組裝行為的探討是現有的文獻中鮮少著墨討論的。經由對照相圖，也可快速搜尋所需水凝膠的成分與濃度。希望此凝膠機制能夠在未來，成為設計促進神經元再生的新材料時有力的工具。" | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-25T03:03:56Z (GMT). No. of bitstreams: 1 U0001-0808202112151300.pdf: 11639093 bytes, checksum: 93200726827c427a3e2f7ef01309e749 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | "口試委員審定書 i 誌謝 ii 摘要 iii ABSTRACT iv CONTENTS vi LIST OF FIGURES x LIST OF TABLES xv Chapter 1 Introduction 1 1.1 Polypeptides-containing amphiphilic block copolymers and their versatile self-assembly nature 1 1.2 Synthesis of polypeptides-containing block copolymers 5 1.3 Monitoring self-assembly structures 11 1.3.1 Versatile and instantly responding self-assembly structures 11 1.3.2 Susceptive structural transformation 13 1.3.3 Manipulating hydrophobic-interaction-induced gelation 14 1.4 Targeted application and rationale of materials selection 24 1.4.1 Poly(γ-benzyl-L-glutamate) (PBG, abbreviated as Bx in copolymers) 24 1.4.2 Poly(L-lysine) (PLL, abbreviated as Ky in copolymers) 25 1.4.3 Poly(ethylene glycol) (PEG, abbreviated as Ez in copolymers) 26 1.5 Motivation, objective and experimental designs 27 Chapter 2 Chemicals, Instruments and Experimental procedures 30 2.1 Chemicals and Instruments 30 2.2 Nomenclature 35 2.3 Synthesis method of polypeptides-containing block copolymers 36 2.3.1 Synthesis of macro-initiator 36 2.3.2 Synthesis of N-carboxyl anhydrides (NCAs) 37 2.3.3 Polymerization and deprotection 38 2.4 Chemical structure characterizations 41 2.4.1 1H Nuclear Magnetic Resonance (NMR) 41 2.4.2 Fourier Transform Infrared Spectroscopy (FTIR) 41 2.4.3 Gel Permeation Chromatography (GPC) 42 2.4.4 Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectroscopy (MALDI-ToF) 42 2.5 Morphology determination 43 2.5.1 Tube-inverting method 43 2.5.2 Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) 43 2.5.3 Dynamic Light Scattering (DLS) 43 2.5.4 Circular Dichroism (CD) 44 2.5.5 Scanning Electron Microscopy (SEM) 44 2.5.6 Small Angle X-ray Scattering (SAXS) 44 2.6 Mechanical properties measurements 44 2.6.1 Rheology study 44 2.6.2 Compression modulus study 45 Chapter 3 Results and Discussion 46 3.1 Synthesis and characteristic of block copolymers 46 3.1.1 Macro-initiator and monomers 46 3.1.2 Block copolymers 56 3.2 Study of Copolymer Morphology in Low Concentration Solution 65 3.2.1 Aggregate formation of amphiphiles in DLS 66 3.2.2 Secondary structures in polypeptide segments 70 3.2.3 Summary of study of polymer morphology in low concentration solution 77 3.3 Study of copolymer morphology study in high concentration solution 78 3.3.1 (Bx-r-Ky)-E34-(Bx-r-Ky) 78 3.3.2 Ky-Bx-E34-Bx-Ky 83 3.3.3 Bx-Ky-E34-Ky-Bx 87 3.3.4 Integration of study of polymer morphology in high concentration solution and self—assembly model purposing 95 3.4 Mechanical properties of polypeptides-containing block copolymers hydrogel 100 3.4.1 Shear modulus and compression modulus 100 3.4.2 Temperature effect 114 Chapter 4 Conclusions 117 Chapter 5 Recommendations 120 5.1 Investigating hydrogel stability in the physiological solution 120 5.2 Systematic study of nanostructure with SAXS 121 5.3 Investigating nano-scale assemblies with cryo-TEM 121 REFERENCE 123 APPENDIX 129" | |
| dc.language.iso | en | |
| dc.subject | 形態學 | zh_TW |
| dc.subject | 水凝膠 | zh_TW |
| dc.subject | 嵌段高分子 | zh_TW |
| dc.subject | 雙親性 | zh_TW |
| dc.subject | 機械性質 | zh_TW |
| dc.subject | 自組裝 | zh_TW |
| dc.subject | 聚胜肽 | zh_TW |
| dc.subject | morphology | en |
| dc.subject | amphiphilic | en |
| dc.subject | self-assembly | en |
| dc.subject | block copolymers | en |
| dc.subject | polypeptide | en |
| dc.subject | mechanical property | en |
| dc.subject | hydrogels | en |
| dc.title | 含有聚胜肽之新穎雙親性嵌段共聚高分子之設計合成與水溶液中自組裝結構之研究 | zh_TW |
| dc.title | "Study of Novel Amphiphilic Block Copolymers Containing Polypeptides: Design, Synthesis and Morphology in Aqueous Solution" | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 林唯芳(Wei-Fang Su) | |
| dc.contributor.oralexamcommittee | 趙基揚(Hsin-Tsai Liu),童世煌(Chih-Yang Tseng) | |
| dc.subject.keyword | 自組裝,聚胜肽,形態學,機械性質,雙親性,嵌段高分子,水凝膠, | zh_TW |
| dc.subject.keyword | self-assembly,polypeptide,morphology,mechanical property,amphiphilic,block copolymers,hydrogels, | en |
| dc.relation.page | 134 | |
| dc.identifier.doi | 10.6342/NTU202102184 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-08-12 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 材料科學與工程學研究所 | zh_TW |
| dc.date.embargo-lift | 2023-08-08 | - |
| 顯示於系所單位： | 材料科學與工程學系 | |
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