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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林璧鳳(Bi-Fong Lin) | |
dc.contributor.author | Victoria Yun-Yun Khor | en |
dc.contributor.author | 許韻韻 | zh_TW |
dc.date.accessioned | 2021-05-20T00:49:28Z | - |
dc.date.available | 2025-08-20 | |
dc.date.available | 2021-05-20T00:49:28Z | - |
dc.date.copyright | 2020-09-15 | |
dc.date.issued | 2020 | |
dc.date.submitted | 2020-08-18 | |
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European Journal of Nutrition, 52(2), 601-608. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/8164 | - |
dc.description.abstract | 綠藻 (chlorella) 是一種單細胞微藻,為目前熟知的保健營養品,具有抗氧化、降血糖、血脂,調節免疫等功能。綠藻含有豐富的營養素,但對於綠藻内的維生素B12,是否為具活性在人體可利用的形式,目前還有爭議。因此,本研究利用動物實驗探討在B12降低的情況下,補充綠藻 (Chlorella sorokiniana) 是否可以改善B12缺乏引起的代謝異常情況。實驗包括實驗一:給予13,21週齡C57BL/6J小鼠AIN-93 (ctrl組) 與B12缺乏飼料15週,後將缺乏組分爲B12缺乏組 (dB12) 和綠藻組 (dB12Ch),餵食28週。實驗二,重複實驗一但小鼠週齡與餵食時間不同。小鼠於9-13週齡開始實驗,餵食AIN-93與B12缺乏飼料17週後,補充綠藻餵食16週。實驗三,給予8週齡C57BL/6J小鼠ctrl組與B12缺乏飼料12週後,分爲B12缺乏組 (dB12) ,1% 綠藻組 (dB12Ch1) 和5% 綠藻組 (dB12Ch5),補充綠藻餵食10週。定期採血,測血糖、血脂和血膽固醇,犧牲後採集性腺脂肪、皮下脂肪、腎周脂肪等測得體脂肪百分率,並測肝臟、腎臟、尿液及糞便的B12含量,和分析脾臟探討補充綠藻對餵予維生素B12缺乏的全身性免疫影響。結果顯示,綠藻可顯著降低血糖、血膽固醇、肝臟膽固醇與三酸甘油酯,也能顯著增加糞便内膽固醇與三酸甘油酯的含量,改善脂質代謝狀況。另外,綠藻也可顯著提高IL-10並降低TNF-α,可能具有免疫調節作用。綠藻的補充顯著增加肝臟與腎臟内維生素B12含量,表示綠藻内的維生素B12可被吸收並儲存於小鼠體内,可作為素食者的維生素B12食物來源。 | zh_TW |
dc.description.abstract | Chlorella is a single-celled microalgae. It is a well-known health supplementation. Chlorella is rich in nutrients, but it is still debatable whether vitamin B12 in Chlorella is an active form which can be used in human body. In this study, we used animal model to investigate whether supplementation of Chlorella sorokiniana can improve metabolic abnormalities caused by B12 deficiency. Experiments included Experiment 1: 13-21-week-old C57BL/6J mice were fed with AIN-93 and B12-deficient diet for 15 weeks, deficient group were divided into B12 deficient group (dB12) and chlorella supplement group (dB12Ch), fed for 28 weeks. Experiment 2: repeat experiment 1, but difference in ages and feeding periods. 9-13-week-old C57BL/6J mice, fed with AIN-93 and B12 deficiency diet for 17 weeks, supplemented with Chlorella for 16 weeks. In Experiment 3, 8-week-old C57BL/6J mice fed AIN-93 and B12-deficient diet for 12 weeks, were divided into B12-deficient group (dB12), 1% chlorella group (dB12Ch1) or 5% chlorella group (dB12Ch5), and fed for 10 weeks. Blood samplings were carried out regularly. Gonadal white adipose tissue, Inguinal white adipose tissue, Retroperitoneal white adipose tissue and Brown adipose tissue were collected after sacrificing to measure the percentage of body fat. The content of B12 in liver, kidney, urine, feces and the spleen were investigated as well as the effect of chlorella on the systemic immunity of vitamin B12 deficiency. The results showed Chlorella can significantly reduce blood sugar, blood cholesterol, liver cholesterol and triglycerides, partially due to significant increase of cholesterol and triglycerides in feces. In addition, Chlorella supplement obviously increase IL-10 and reduce TNF-α, which may have immunomodulatory effects. The vitamin B12 content in the liver and kidney were significantly elevated after supplement of Chlorella, indicating that vitamin B12 in Chlorella can be absorbed and stored in mice. It suggest that Chlorella may be the source of vitamin B12 for vegans. | en |
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dc.description.tableofcontents | 中文摘要 i Abstract ii 目錄 iii 圖目錄 vii 表目錄 ix 縮寫對照表 x 第一章、緒論 1 第一節 文獻回顧 1 一、 維生素B12 1 (一) 簡介 1 (二)維生素B12與葉酸的交互作用 3 (三)維生素B12與脂質代謝 4 (四)維生素B12與腎臟疾病 5 (五)維生素B12缺乏 5 二、 素食 8 三、 藻類 9 (一)微藻 9 (二)應用 9 四、 綠藻 9 (一)簡介 9 (二) 綠藻的營養成分 11 (三)綠藻功能的相關研究 12 五、 免疫 13 (一)維生素B12與免疫反應及氧化壓力的影響 14 (二)綠藻與免疫的相關研究 14 第二節 研究動機與目的 16 第二章、實驗設計與材料方法 18 第一節 實驗設計與分組 18 第二節 動物飼養 19 第三節 實驗方法 22 一、 血清、尿液與糞便收集 22 (一)血清收集 22 (二)糞便收集 22 (三)尿液收集 22 二、 組織處理 22 (一)肝臟與腎臟 22 (二)肝臟脂質 23 三、 樣品分析 23 (一)蛋白質檢測 23 (三) 腎病變指標檢測 24 1. 尿液肌酸酐檢測 24 2. 血清尿素氮檢測 24 (四) 維生素B12含量測定 24 1. 血清與尿液B12測定 24 2. 組織B12含量萃取與測定 25 3. 糞便B12含量萃取與測定 26 (四)血清與綠藻葉酸測定 26 (五)醣類與脂質檢測 27 1. 血糖分析 27 2. 血清與肝臟三酸甘油酯分析 27 3. 血清與肝臟膽固醇分析 28 4. 糞便脂質萃取與測定 28 (六)體脂肪百分率計算 28 (七)脾臟細胞單離與培養 29 1. 淋巴細胞分離 29 2. 細胞計數 30 3. 免疫細胞培養 30 4. 細胞激素含量分析 30 (八)統計分析 31 第三章 實驗結果 32 一、 小鼠體重與攝食量 32 二、 小鼠器官重量 33 三、 體脂肪比率 35 四、 小鼠組織、血清、尿液與糞便B12 36 五、 餵予B12缺乏與綠藻對小鼠血清葉酸之影響 40 六、 餵予B12缺乏與補充綠藻對脂質的影響 41 七、 糞便脂質分析 43 八、 餵予B12缺乏和補充綠藻對血糖之影響 44 九、 餵予B12缺乏和補充綠藻對小鼠腎臟功能的影響 45 十、 餵予B12缺乏和補充綠藻對脾臟細胞活性的影響 46 第四章 綜合討論與結論 47 第一節 討論 47 一、 餵予維生素B12缺乏與補充綠藻對老年小鼠之影響(實驗一和二) 47 二、 餵予維生素B12缺乏與補充綠藻對實驗三小鼠之影響 50 (一)器官重與體脂肪 50 (二)維生素B12之影響 50 (三)補充綠藻對餵予B12缺乏小鼠脂質代謝和血糖之影響 55 (四)脾臟免疫的影響 56 三、 低劑量、中劑量與高劑量綠藻對整體的影響 57 第二節 結論 59 參考文獻 60 附錄 67 圖1-1維生素B12之化學結構 2 圖1-2維生素B12之功能與葉酸循環。 4 圖1-3綠藻的分類 10 圖3-1餵食缺B12飼料與補充綠藻後小鼠的體脂肪比率。(a) 實驗一、(b) 實驗二、(c) 實驗三 35 圖3-2餵食缺B12飼料與補充綠藻後小鼠肝臟的維生素B12含量。(a) 實驗一、(b) 實驗二、(c) 實驗三 36 圖3-3餵食缺B12飼料與補充綠藻後小鼠腎臟的維生素B12含量。(a) 實驗一、(b) 實驗二、(c) 實驗三 37 圖3-4餵食缺B12飼料與補充綠藻後小鼠血清的維生素B12含量。(a) 實驗一、(b) 實驗二、(c) 實驗三 38 圖3-5餵食缺B12飼料與補充綠藻對小鼠尿液糞便維生素B12之影響。(a) 實驗一、(b) 實驗二、(c) 實驗三 39 圖3-6餵食缺B12與補充綠藻對小鼠血清葉酸的影響。(a) 實驗一、(b) 實驗二、(c) 實驗三 40 圖3-7餵食缺B12和補充綠藻對小鼠血清和肝臟三酸甘油酯之影響。(a, b) 實驗一、(c, d) 實驗二、(e, f) 實驗三 41 圖3-8餵食缺B12和補充綠藻對血清與肝臟膽固醇之影響。(a,b) 實驗一、(c, d) 實驗二、(e, f) 實驗三 42 圖3-9餵食缺B12和補充綠藻對實驗三糞便的 (a) 排出量, (b, c) 三酸甘油酯及 (d, e) 膽固醇含量 43 圖3-10餵食缺B12和補充綠藻對禁食血糖之影響。(a) 實驗一、(b) 實驗二、(c) 實驗三 44 圖3-11餵食缺B12和補充綠藻對小鼠(a, c, e) 尿蛋白和 (b, d, f) 血清尿素氮之影響。(a-b) 實驗一、(c-d) 實驗二、(e-f) 實驗三 45 圖 4-1餵食缺B12飼料12週和犧牲後22週血清維生素B12的含量。 52 表1-1 維生素B12的生化指標 7 表1-2 素食的種類 8 表 2-1實驗一和實驗二飼料組成 20 表2-2實驗三飼料組成 21 表3-1餵食不同飼料小鼠體重及攝食量 32 表3-2餵予缺乏維生素B12和補充綠藻對小鼠絕對器官重量之影響 33 表3-3餵予缺乏維生素B12和補充綠藻對小鼠相對器官重量之影響 34 表3-4 餵予B12缺乏和補充綠藻對小鼠脾臟免疫細胞之影響。 46 表4-1實驗小鼠於實驗一和實驗二中各組的年齡分配 47 表4-2不同劑量的綠藻在餵予缺B12飼料下之影響 57 | |
dc.language.iso | zh-TW | |
dc.title | 補充綠藻對C57BL/6J小鼠攝食維生素B12缺乏飼料的影響 | zh_TW |
dc.title | The effect of Chlorella sorokiniana supplementation on C57BL/6J mice fed with vitamin B12-deficient diets | en |
dc.type | Thesis | |
dc.date.schoolyear | 108-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 許瑞芬,陳玉華,洪永瀚,鄭光成 | |
dc.subject.keyword | 素食,維生素B12缺乏,綠藻,脂質代謝,免疫, | zh_TW |
dc.subject.keyword | Vegans,vitamin B12 deficiency,Chlorella sorokiniana,lipid metabolism,immunology, | en |
dc.relation.page | 67 | |
dc.identifier.doi | 10.6342/NTU202003734 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2020-08-19 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
dc.date.embargo-lift | 2025-08-20 | - |
顯示於系所單位: | 生化科技學系 |
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