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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 曲芳華(Fang-Hua Chu) | |
| dc.contributor.author | Chong-Yao Hong | en |
| dc.contributor.author | 洪崇耀 | zh_TW |
| dc.date.accessioned | 2021-05-20T00:49:28Z | - |
| dc.date.available | 2025-08-17 | |
| dc.date.available | 2021-05-20T00:49:28Z | - |
| dc.date.copyright | 2020-08-21 | |
| dc.date.issued | 2020 | |
| dc.date.submitted | 2020-08-18 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/8163 | - |
| dc.description.abstract | 紅檜 (Chamaecyparis formosensis Matsum.)為臺灣特有的珍貴樹種，其精油成分以萜類化合物 (Terpenoids) 為主，並已被證實有多種優異的生物活性，故對紅檜的萜類合成酶 (Terpene synthase, TPS) 及細胞色素P450 (Cytochrome P450) 的功能研究更顯重要，未來可以應用於重要成分的半化學合成。且柏科於P450的研究亦較闕如，若能鑑定P450功能亦能協助裸子植物演化途徑的解析。本研究自紅檜葉部轉錄體的NGS (Next generation sequence) 資料庫取得4個倍半萜合成酶：Cf_6163、Cf_17788_1、Cf_17788_2及Cf_30044；以及3個P450的序列：Cf_791、Cf_9283及Cf_12832。其中Cf_6163、Cf_17788_1及Cf_17788_2各與FPP受質反應出Germacrene A、新化合物以及Isogermacrene A，其中新化合物被鑑定為1,2,3,5,6,7,8,8a-Octahydro-8a-methyl-1-methylene-7-(1-methylethyl) naphthalene，Cf_30044則未成功完成催化反應。Cf_17788_1/2的產物，可能具有特殊的環化機制，未來透過點突變試驗可對其環化機制有更進一步的了解。3個P450中，Cf_791屬於CYP706M亞族、Cf_9283及Cf_12832各屬於CYP76AA亞族。P450與TPS在大腸桿菌 (Escherichia coli) 的共表現系統僅有Cf_6163與Cf_12832有出現新的產物。未來可採用其他表現系統，並將其他TPS及P450納入試驗，以得到準確的P450功能鑑定。 | zh_TW |
| dc.description.abstract | Chamaecyparis formosensis Matsum.is a precious tree species unique to Taiwan. Its essential oil components are mainly terpenoids. It has been confirmed to have a variety of excellent biological activities. Therefore, it is important on the research of functional characterization of terpene synthase (TPS) and CYP450 (Cytochrome P450). The research can be applied to the semi-chemical synthesis of important components in the future. On the other hand, the study of P450 from Cupressaceae is also lacking. Once the function of P450 can be identified, it can also help the analysis of evolutionary pathway of gymnosperms. In this study, four sesquiterpene synthases were obtained from the NGS (Next generation sequence) database of Chamaecyparis formosensis Matsum leaf transcriptome: Cf_6163, Cf_17788_1, Cf_17788_2 and Cf_30044; and three P450 sequences: Cf_791, Cf_9283 and Cf_12832. Among them, Cf_6163, Cf_17788_1 and Cf_17788_2 reacted with FPP substrate to produce Germacrene A, a new compound and Isogermacrene A. The new compound was identified as 1,2,3,5,6,7,8,8a-Octahydro-8a-methyl- 1-methylene-7-(1-methylethyl) Naphthalene. Cf_30044 failed to complete the catalytic reaction. The product of Cf_17788_1/2 may have a special cyclization mechanism. In the future, a point mutation test can be used to further understand their cyclization mechanism. Among the three P450s, Cf_791 belongs to the CYP706M subfamily, while Cf_9283 and Cf_12832 belong to the CYP76AA subfamily. In the co-expression system of P450 and TPS in Escherichia coli, only Cf_6163 and Cf_12832 had a new product. In the future, other expression systems can be used, and other TPSs and P450s can be included in the test to obtain accurate P450 functional identification. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-20T00:49:28Z (GMT). No. of bitstreams: 1 U0001-1708202014211900.pdf: 5465449 bytes, checksum: 8c583049220ce01196d80ac0e8577e3b (MD5) Previous issue date: 2020 | en |
| dc.description.tableofcontents | 口試委員審定書 i 誌謝 ii 摘要 iii Abstract iv 目錄 vi 圖目錄 ix 表目錄 xi 一、前言 1 二、文獻回顧 4 2.1 萜類化合物介紹 4 2.1.1萜類化合物 4 2.1.2植物中的功能：以植物防禦為例 5 2.2 萜類化合物的生合成 7 2.2.1萜類化合物的生合成途徑 7 2.2.2萜類化合物的環化途徑：以倍半萜類化合物為例 10 2.3 萜類合成酶的分類 12 2.3.1萜類合成酶的受質 12 2.3.2萜類合成酶的離子化機制 12 2.3.3萜類合成酶的內含子分布 15 2.3.4萜類合成酶的演化分群 16 2.4 細胞色素P450的介紹 17 2.5 P450與植物演化 22 2.6 P450對萜類化合物的修飾 24 2.6.1 初級萜類代謝相關的P450家族 24 2.6.2 初級萜類代謝相關的P450家族 25 三、材料方法 30 3.1 試材及紅檜葉部RNA萃取 30 3.2 選殖基因的篩選 30 3.3葉部基因的選殖 31 3.4蛋白質質體建構 32 3.5西方墨點法 (Western blot) 34 3.6 萜類合成酶蛋白質大量表現及純化 35 3.7目標蛋白質與受質反應 37 3.8大腸桿菌 in vivo co-expression反應系統 38 3.9以氣相層析質譜儀 (GC-MS) 分析產物 38 3.10基因序列演化地位分析 39 3.11蛋白質相關資訊預測 39 3.12 NMR (Nuclear Magnetic Resonance) 解析倍半萜化合物結構 40 3.13 Genomic DNA 萃取及內含子序列取得 40 四、結果 41 4.1 萜類合成酶選殖資訊 41 4.2 萜類合成酶內含子分布狀況 44 4.3 萜類合成酶系統發生樹 46 4.4 萜類合成酶西方墨點結果 48 4.5 萜類合成酶酵素純化 49 4.6 萜類合成酶 in vitro反應 51 4.7 萜類合成酶 in vivo 反應 56 4.8 NMR解析結果 58 4.9 萜類合成酶模擬結構 62 4.10 P450選殖資訊 63 4.11 P450系統發生樹 67 4.12 P450 西方墨點結果 68 4.13 P450 Coexpression 69 五、討論 70 5.1 萜類合成酶產物 70 5.1.1 產物於植物體中的分布狀況 70 5.1.2 萜類化合物之生物活性及應用 74 5.2萜類合成酶的環化機制及重要反應殘基 75 5.3 內含子分布情形 80 5.4 系統發生樹分群 81 5.5 P450反應結果 83 5.5.1系統表現 83 5.5.2其他家族 85 六、結論 87 七、參考資料 88 | |
| dc.language.iso | zh-TW | |
| dc.title | 紅檜葉部倍半萜合成酶及細胞色素P450的基因選殖和功能鑑定 | zh_TW |
| dc.title | Cloning and Characterization of Sesquiterpene Synthases and Cytochrome P450 from Leaves of Chamaecyparis formosensis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 108-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 何政坤(Cheng-Kuen Ho),孫英玄(Ying-Hsuan Sun),林盈仲(Ying-Chung Lin),王升陽(Sheng-Yang Wang) | |
| dc.subject.keyword | 紅檜,萜類合成酶,細胞色素P450,倍半萜類化合物,細胞色素P450表現系統, | zh_TW |
| dc.subject.keyword | Chamaecyparis formosensis Matsum,Terpene synthase,Cytochrome P450,Sesquiterpenoid,P450 Expression system, | en |
| dc.relation.page | 100 | |
| dc.identifier.doi | 10.6342/NTU202003745 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2020-08-19 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 森林環境暨資源學研究所 | zh_TW |
| dc.date.embargo-lift | 2025-08-17 | - |
| 顯示於系所單位： | 森林環境暨資源學系 | |
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