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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 林順福(Shun-Fu Lin) | |
| dc.contributor.author | Po-Chun Lin | en |
| dc.contributor.author | 林伯駿 | zh_TW |
| dc.date.accessioned | 2022-11-24T09:24:29Z | - |
| dc.date.available | 2022-11-24T09:24:29Z | - |
| dc.date.copyright | 2021-11-06 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-10-05 | |
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(2016). Development of commercial thermo-sensitive genic male sterile rice accelerates hybrid rice breeding using the CRISPR/Cas9-mediated TMS5 editing system. Scientific Reports, 6(1), 1-12. Zhou, H., Zhou, M., Yang, Y., Li, J., Zhu, L., Jiang, D., Dong, J., Liu, Q., Gu, L., Zhou, L., Feng, M., Qin, P., Hu, X., Song, C., Shi, J., Song, X., Ni, E., Wu, X., Deng, Q., Liu, Z., Chen, M., Liu, Y. G., Cao, X., Zhuang, C. (2014). RNase ZS1 processes UbL40 mRNAs and controls thermosensitive genic male sterility in rice. Nature Communications, 5(1), 1-9. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/81589 | - |
| dc.description.abstract | 使用溫敏性雄不稔特性以育成兩系雜交水稻品種可有效利用雜種優勢以提高水稻產量，而目前臺灣仍未建立穩定生產兩系雜種水稻之系統。因此，本研究目的為定位溫敏性雄不稔數量性狀基因座，以探討其遺傳機制，並選拔出溫敏性雄不稔品系作為兩系雜交用之母本，同時探討影響稔實率之雜交不親和性等性狀。本研究以具溫敏性雄不稔性狀之台大品系A與稉稻台南13號雜交產生F1再自交所獲得164株之F2族群為材料，並以129個SSR分子標誌建立連鎖圖譜，提高分子標誌之密度。結合分子標誌基因型與稔實率等外表型進行數量性狀基因定位，結果共測得4個與稔實率相關之QTL：其中位於第1對染色體之QTL於常溫環境下測得，為影響秈稻與稉稻亞種間雜交不親和之QTL，且與前人所測之位置相近；而於第3對與第11對染色體上定位到溫敏性雄不稔QTL，且分別與不同前人測得之溫敏性雄不稔QTL位置相符合，與此兩QTL緊密連鎖之分子標誌可應用於選育兩系水稻雜交系統之具有雄不稔特性之母本品系；而定位於第6對染色體上之QTL由於其影響稔實率之對偶基因來自稉稻父本台南13號，推測此基因座為耐高溫相關之基因座，若將此QTL中之秈稻對偶基因導入稉稻品系中，可望提升臺灣稉稻品系之耐高溫程度，以因應全球氣候暖化。此外，利用上述兩溫敏性雄不稔數量性狀基因座緊密連鎖之分子標誌進行雄不稔個體選拔，獲選稔實率為0%個體均符合緊密連鎖分子標誌之基因型，顯示此兩分子標誌可作為未來選拔雄不稔品系之優良工具。本研究亦定位與雜種水稻生產相關之紫色葉鞘性狀與抽穗期等性狀，可供生產雜交種之參考。綜合影響稔實率之4個QTL分析結果，本研究共推薦6個溫敏性雄不稔品系與6個耐高溫品系，可作為建立水稻兩系雄不稔系統之材料。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-24T09:24:29Z (GMT). No. of bitstreams: 1 U0001-1309202113290100.pdf: 2033050 bytes, checksum: ce184a400a4743dc12a3eb59385015f8 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | "致謝 i 中文摘要 ii Abstract iii 表目錄 vii 圖目錄 viii 前言 1 前人研究 3 一、水稻雄不稔相關研究 3 二、水稻雜交不親和性與雜種優勢相關研究 5 三、紫色葉鞘相關研究 7 四、抽穗期相關研究 7 五、研究目的 8 材料與方法 9 一、試驗材料 9 二、外表型調查 10 （一）稔實率（fertility rate）性狀調查 10 （二）雜交不親和性（hybrid-incompatibility, HI）性狀調查 10 （三）紫色葉鞘（purple leaf sheath, PLS）性狀調查 10 （四）抽穗期（days to heading, HD）性狀調查 10 三、基因型分析 11 （一）水稻DNA萃取 11 （二）簡單重複序列分子標誌引子合成 11 （三）聚合酶連鎖反應及瓊脂膠電泳分析 11 四、數量性狀基因座定位與分析 12 （一）連鎖圖譜之建立 12 （二）數量性狀基因座定位 13 五、雄不稔以及雜交不親和性之遺傳背景分析 13 結果 14 一、連鎖圖譜之建立 14 二、基因型比例分佈 18 三、外表型分佈 21 （一）稔實率 21 （二）雜交不親和性 23 （三）紫色葉鞘 24 （四）抽穗期 25 四、數量性狀基因座定位與遺傳分析 26 （一）稔實率之基因定位 26 （二）雜交不親和性之基因定位 31 （三）紫色葉鞘之基因定位 34 （四）抽穗期之基因定位 37 五、雄不稔以及雜交不親和性與遺傳背景之相關性 41 （一）雄不稔與遺傳背景之相關性 41 （二）雜交不親和性與遺傳背景之相關性 45 六、優良個體之推薦與後續追蹤 47 討論 49 一、連鎖圖譜之建立 49 二、基因型比例分佈 50 三、數量性狀基因座位置測定 51 （一）稔實率 51 （二）雜交不親和性 55 （三）紫色葉鞘 56 （四）抽穗期 57 三、雄不稔以及雜交不親和與遺傳背景之相關性 58 （一）雄不稔與遺傳背景之相關性 58 （二）雜交不親和性與遺傳背景之相關性 59 （三）遺傳背景之研究 59 四、優良植株之推薦與後續追蹤 60 五、目前面臨之難題與未來展望 60 結論 62 參考文獻 63 " | |
| dc.language.iso | zh-TW | |
| dc.subject | 水稻 | zh_TW |
| dc.subject | 雄不稔 | zh_TW |
| dc.subject | 雜交不親和性 | zh_TW |
| dc.subject | male sterility | en |
| dc.subject | incompatibility | en |
| dc.subject | rice | en |
| dc.title | 水稻雄不稔與亞種間雜交不親和性之遺傳機制 | zh_TW |
| dc.title | Genetic Mechanisms of Male Sterility and Intersubspecific Incompatibility in Rice | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 賴明信(Hsin-Tsai Liu),陳榮坤(Chih-Yang Tseng) | |
| dc.subject.keyword | 雄不稔,雜交不親和性,水稻, | zh_TW |
| dc.subject.keyword | male sterility,incompatibility,rice, | en |
| dc.relation.page | 77 | |
| dc.identifier.doi | 10.6342/NTU202103144 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2021-10-07 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農藝學研究所 | zh_TW |
| Appears in Collections: | 農藝學系 | |
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| File | Size | Format | |
|---|---|---|---|
| U0001-1309202113290100.pdf Restricted Access | 1.99 MB | Adobe PDF |
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