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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/81156完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 楊啓伸(Chii-Shen Yang) | |
| dc.contributor.author | Kuan-Lin Chen | en |
| dc.contributor.author | 陳冠霖 | zh_TW |
| dc.date.accessioned | 2022-11-24T03:33:24Z | - |
| dc.date.available | 2021-08-11 | |
| dc.date.available | 2022-11-24T03:33:24Z | - |
| dc.date.copyright | 2021-08-11 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-08-09 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/81156 | - |
| dc.description.abstract | "雙元協同訊息傳遞系統（Two-component system，TCS) 在微生物中負責偵測環境中的刺激，並對不同刺激產生專一的反應，協助微生物在多變的環境下生存。例如，細菌會利用TCS來感測環境中的營養成分或是有害物質的濃度高低，進一步改變泳動的行為，稱為趨化性（Chemotaxis）。在嗜鹽古菌中，則會透過TCS系統裡的感受型視紫質(sensory rhodopsin, SR)來偵測不同波長的光，產生趨光（attractant）或避光（repellent）的泳動行為，此稱為趨光性（phototaxis）。此趨光性反應之產生，需要透過可吸收偏紅光波長的SRI (sensory rhodopsin I)進行趨光，以及吸收偏藍光的SRII (sensory rhodopsin II)進行避光來擷抗；目前已知兩者皆藉由調控其 TCS 下游的傳導元（transducer），分別為 HtrI 與 HtrII，來與Che 系列蛋白質進行交互作用。除了上述提到的SRI及SRII外，先前研究發現在 Haloarcula marismortui 中還存在第三種可以感測綠光的 sensory rhodopsin M（SRM）以及其專屬傳導元， HtrM。並且在後續研究中發現，綠光可以同時抑制趨光/避光行為，但在只有表現SRI 和 SRII 的菌體時，綠光不會抑制此菌體的趨光/避光行為。為了解綠光使古菌的泳動光趨性受抑制的機制，本論文試圖提出假設，說明SRM-HtrM 如何抑制 SRII-HtrII 的避光和 SRI-HtrI 的趨光泳動行為。首先經由序列分析，發現HtrM的胺基酸長度僅有HtrI 和 HtrII 的一半不到，而且含有類似於HtrI 與HtrII 中的 HAMP 區域。而 HAMP 區域是 HtrI、HtrII與 CheW/CheA 蛋白質間的互觸區 (interaction region)。因為已知被 HtrI 與HtrII 活化的CheA，可以同時進一步磷酸化 CheY 和 CheB。而磷酸化的 CheY 會控制鞭毛轉動的方向，使菌體避光；磷酸化的 CheB則是產生負回饋作用，會去除HtrI與HtrII在HAMP 區域上的甲基。因此本論文決定透過解析目前科學界仍無法解出的 HmCheB完整結構，並依其結構來解釋細菌的趨光性行為，目前提出兩個假設：一、 HtrM可能會和兩個 Htr 競爭磷酸化的 CheB，但因不產生原先 HtrI、HtrII 去甲基化後的訊號，使得磷酸化的CheB所誘發的負回饋機制失效，菌體因此誤認為需要更多 CheB 被磷酸化，因此讓和 CheB競爭磷酸化的 CheY 無法有效的被磷酸化，進而導致 CheY 原本可以驅動避光或趨光行為的效用下降。二、被活化的HtrM，可能提高了與CheA或CheW的親和力，使得可以參與磷酸化CheY和CheB的CheA數量下降，導致CheY去驅動避光鞭毛轉動的效率下降，因而降低了避光或趨光的能力。以上兩個假設皆涉及 CheB 和其他蛋白質的交互作用。本研究成功獲得 CheB 的完整蛋白質結構，並冀望透過對 CheB 整體結構的瞭解，替未來 CheB 和其他蛋白質交互作用的研究，以及細菌趨光性泳動的機制探討，提供堅實的研究基礎。" | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-24T03:33:24Z (GMT). No. of bitstreams: 1 U0001-0608202112262600.pdf: 7211537 bytes, checksum: c560753d922a7cfa7461f07326761ccb (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 目錄 I 圖目錄 IV 表目錄 V 摘要 VI Abstract VIII 第一章 緒論 1 第一節 嗜鹽古生菌之介紹 1 第二節 微生物視紫質(Microbial Rhodopsin) 1 第三節 古生菌感覺型視紫質 3 第四節 傳導元的結構與特性 3 第五節 Two-component system 5 第六節 Che proteins 調控機制 7 第七節 嗜鹽古生菌Haloarcula marismortui 奇特的六感光視紫質系統 9 第八節 三顏色的感覺型視紫質系統及其 two-component system 的胺基酸序列之初步研究 12 第九節 機制假說 14 第十節 實驗設計及流程 15 第十一節 嗜鹽古生菌Haloarcula marismortui之CheB及嗜熱菌CheB之regulatory domain catalytic domain 16 第十二節 本論文目標：解出CheB整體結構 17 第二章材料與方法 20 第一節 生物試劑 20 2-1-1 菌種 20 2-1-2 質體 20 第二節 藥品 21 第三節 儀器設備 23 2-3-1 核酸電泳 23 2-3-2 蛋白質電泳及轉印 23 2-3-3 離心機 23 2-3-4 酸鹼度計 23 2-3-5 其他 23 第四節 實驗方法 24 2-4-1 生物資訊學分析 24 2-4-1.1 基因資料庫 24 2-4-1.2 蛋白質功能預測分析 24 2-4-1.3 蛋白質結構分析 24 2-4-2 DNA之建構與轉形 24 2-4-2.1 小量核酸萃取 24 2-4-2.2 聚合酶鏈鎖反應(PCR) 25 2-4-2.3 DNA膠體純化 25 2-4-2.4 限制酶裁切 25 2-4-2.5 DNA接合 25 2-4-2.6 大腸桿菌轉形 25 2-4-2.7 轉形菌株鑑定 26 2-4-2.8 建構重組蛋白質 26 2-4-3 重組蛋白質表現及純化 26 2-4-3.1 重組可溶蛋白質表現 26 2-4-3.2 重組可溶蛋白質純化 27 2-4-4 蛋白質定量及定性 27 2-4-4.1蛋白質定量 27 2-4-4.2蛋白質變性電泳 27 2-4-5 蛋白質結晶與X光繞射實驗 28 2-4-5.1 蛋白質點晶(hanging drop method) 28 2-4-5.2 蛋白質點晶(sitting drop method) 29 2-4-5.3 結晶結構(X光繞射實驗) 29 第三章 實驗結果 30 第一節 HmCheB初步分析 30 第二節 HmCheB重組蛋白質表現與純化 32 3-2-1 GST-HmCheB之序列 32 3-2-2 GST-HmCheB表現與純化(SDS-PAGE) 33 第三節 GST-HmCheB蛋白質結晶(sitting drop method) 34 第四節 GST-HmCheB蛋白質晶體X光繞射實驗 35 第五節 Rosetta計算理論蛋白質結構 36 第六節 GST-HmCheB結構決定分析和 refinement 迴圈策略 39 第四章 結論與討論 43 第一節 感覺型視紫質與傳導元之構形變化分析 43 第二節 HmHtrM序列分析 51 第三節 HmCheB功能區域分析 52 第四節 HmCheB與HmHtrM之HAMP domain分析 60 第五章 未來展望 63 第六章 參考文獻 65 | |
| dc.language.iso | zh-TW | |
| dc.subject | Che蛋白質 | zh_TW |
| dc.subject | 嗜鹽方形古菌 | zh_TW |
| dc.subject | 光趨性 | zh_TW |
| dc.subject | 雙元協同訊息傳遞系統 | zh_TW |
| dc.subject | Che proteins | en |
| dc.subject | Haloarcula marismortui | en |
| dc.subject | phototaxis | en |
| dc.subject | two-component system | en |
| dc.title | 對死海嗜鹽古菌雙元協同訊息傳遞系統中的CheB蛋白質之全結構解析與生理功能推測 | zh_TW |
| dc.title | Resolving The Whole Structure And Physiological Function Prediction Of A CheB Protein In The Two-Component System From Haloarcula marismortui | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.author-orcid | 0000-0003-4369-2871 | |
| dc.contributor.oralexamcommittee | 鄭貽生(Hsin-Tsai Liu),李昆達(Chih-Yang Tseng),吳亘承 | |
| dc.subject.keyword | 嗜鹽方形古菌,光趨性,雙元協同訊息傳遞系統,Che蛋白質, | zh_TW |
| dc.subject.keyword | Haloarcula marismortui,phototaxis,two-component system,Che proteins, | en |
| dc.relation.page | 76 | |
| dc.identifier.doi | 10.6342/NTU202102143 | |
| dc.rights.note | 同意授權(限校園內公開) | |
| dc.date.accepted | 2021-08-10 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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