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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/80807完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林俊彬(CHUN-PIN LIN) | |
| dc.contributor.author | Rupali Bhorade | en |
| dc.contributor.author | 白儒曼 | zh_TW |
| dc.date.accessioned | 2022-11-24T03:17:17Z | - |
| dc.date.available | 2021-11-05 | |
| dc.date.available | 2022-11-24T03:17:17Z | - |
| dc.date.copyright | 2021-11-05 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-10-07 | |
| dc.identifier.citation | 1. Ahmed, G.M., et al., Tissue engineering approaches for enamel, dentin, and pulp regeneration: an update. Stem cells international, 2020. 2020: p. 1-15 2. Hashemi-Beni, B., et al., Tissue engineering: dentin–pulp complex regeneration approaches (a review). Tissue and Cell, 2017. 49(5): p. 552-564. 3. Huang, G.T., Pulp and dentin tissue engineering and regeneration: current progress. Regenerative medicine, 2009. 4(5): p. 697-707. 4. Jung, C., et al., Pulp-dentin regeneration: current approaches and challenges. Journal of tissue engineering, 2019. 10: p. 2041731418819263. 5. Yang, J., G. Yuan, and Z. Chen, Pulp regeneration: current approaches and future challenges. Frontiers in physiology, 2016. 7: p. 58. 6. Kim, J.Y., et al., Regeneration of dental-pulp-like tissue by chemotaxis-induced cell homing. Tissue Engineering Part A, 2010. 16(10): p. 3023-3031. 7. Moussa, D.G. and C. 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/80807 | - |
| dc.description.abstract | 組織工程在牙科領域從根本上改變了根管治療師評估治療方案的方式。我們先前的研究發現，含有奎塞汀的中孢子鈣矽酸鹽/硫酸鈣（MSCSQ）可以誘導硬組織缺陷區域再生和新肌形成。這項研究的重點是MSCSQ支架是否也能有效地調節牙本質再生。在這項研究中，我們使用3D列印技術製造了MSCSQ複合腳手架。通過掃描電子顯微鏡（SEM）檢查了MSCSQ支架的特徵，並評估了機械特性。此外，我們評估了不同支架上培養的人類牙髓幹細胞（hDPSC）的細胞生存能力、細胞增殖、與牙本質再生相關的蛋白質表達和礦化行為。 我們發現球形石在短時間內迅速沉澱在支架表面。細胞行為的體外（in-vitro）結果顯示，帶有MSCSQ支架的hDPSC的細胞生存能力顯著高於帶有MSCS支架的hDPSC的細胞。此外，在MSCSQ支架上培養了hDPSC之後，特定牙本質生成的標誌，如DSPP和DMP-1蛋白，被明顯地誘導出。此外，與MSCS支架相比，MSCSQ支架還具有增強的牙本質再生性和免疫抑制性能。這些結果表明，MSCSQ支架可被視為臨床應用和牙齦再生的潛在生物安全架。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-24T03:17:17Z (GMT). No. of bitstreams: 1 U0001-0510202111421300.pdf: 2164002 bytes, checksum: e8fbc94e9b10ab27476fc3e8ad0cdf5e (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | "Chapter 1: Introduction 1 1.1 Need for pulp regeneration 1 1.2 Definition and fundamental characteristics of regenerated pulp tissue 2 1.3 Current approaches of pulp regeneration 4 1.3.1 Cell homing strategy 4 1.3.2 Cell transplantation strategy 7 1.4 Drawbacks of current pulp regeneration techniques 8 1.5 Consideration to be taken for regeneration of healthy pulp-dentin complex 12 1.6 Future challenges 13 Chapter 2: Literature review 15 2.1 Stem Cells 17 2.2 Bioactive agents-growth factors (GF) 18 2.2.1 Herbal extract quercetin (Figure 2.3) 18 2.3 Scaffolds 22 2.3.1 Why use scaffolds? 22 2.3.2 Types of scaffolds: 22 2.3.3 Scaffolds in Regenerative Endodontics 24 2.3.4 Ideal Requirements of Scaffold 26 2.3.5 Characteristics of polycaprolactone scaffold PCL 27 2.4 3D printing 29 2.4.1 Importance of 3D printing technique for scaffold printing 29 Chapter 3: Purpose of study and study design 32 3.1 Research Goal 32 3.2 Hypothesis 32 3.3 Specific Aim 32 3.4 Study design 33 Chapter 4: Materials and methods 35 4.1 Development of quercetin-contained MSCS scaffold 35 4.1.1 Synthesis and characterization of MSCS composites 35 4.1.2 Preparation of scaffolds 36 4.1.3 In Vitro Soaking 37 4.2 Evaluation of the characteristics of the scaffold 37 4.2.1 X-ray diffraction (XRD) and stress-strain curve 37 4.2.2 Contact angle 37 4.3 Effect of quercetin-contained MSCS scaffold on human dental pulp stem cells (hDPSCs) 37 4.3.1 Cell Culture 37 4.3.2 Cytotoxicity Assay 38 4.3.3 Cell Adhesion and Cell Viability Assay (using MG63 cells) 38 4.3.4 Cell viability and proliferation (using hDPSCs) 39 4.3.5 Enzyme-linked immunosorbent assay 39 4.3.6 Calcium deposition 40 4.3.7 Statistical analysis 40 Chapter 5: Results 41 5.1 Scaffold characteristics 41 5.1.1 Physical characteristics of the scaffold 41 5.1.2 Mechanical properties and microstructure 45 5.1.3 Contact angle 48 5.2 Cytotoxicity, proliferation, and inflammatory marker 49 5.3 Odontogenesis behaviors 56 Chapter 6: Discussion 59 Chapter 7: Conclusion 69 References 72" | |
| dc.language.iso | en | |
| dc.subject | 孔矽酸鈣 | zh_TW |
| dc.subject | 槲皮素 | zh_TW |
| dc.subject | 三維列印 | zh_TW |
| dc.subject | 3D printing | en |
| dc.subject | mesoporous calcium silicate/ calcium sulfate | en |
| dc.subject | odontogenesis | en |
| dc.subject | Quercetin | en |
| dc.subject | dentin regeneration | en |
| dc.title | 研發三維列印中孔矽酸鈣陶瓷支架搭載天然槲皮素萃取物應用於牙本質再生 | zh_TW |
| dc.title | Development of mesoporous calcium silicate scaffold combined with herbal extract (quercetin) created by using a 3D printing technique for Dentinogenesis | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.advisor-orcid | 林俊彬(0000-0002-3498-1113) | |
| dc.contributor.oralexamcommittee | 劉緒宗(Hsin-Tsai Liu),陳文章(Chih-Yang Tseng),謝明佑,章浩宏 | |
| dc.subject.keyword | 槲皮素,三維列印,孔矽酸鈣, | zh_TW |
| dc.subject.keyword | Quercetin,3D printing,dentin regeneration,odontogenesis,mesoporous calcium silicate/ calcium sulfate, | en |
| dc.relation.page | 80 | |
| dc.identifier.doi | 10.6342/NTU202103555 | |
| dc.rights.note | 同意授權(限校園內公開) | |
| dc.date.accepted | 2021-10-07 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
| 顯示於系所單位： | 臨床牙醫學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| U0001-0510202111421300.pdf 授權僅限NTU校內IP使用（校園外請利用VPN校外連線服務） | 2.11 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。