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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/80527完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳敏慧(Min-Huey Chen) | |
| dc.contributor.author | Chun-Yen Lin | en |
| dc.contributor.author | 林君諺 | zh_TW |
| dc.date.accessioned | 2022-11-24T03:08:36Z | - |
| dc.date.available | 2021-11-02 | |
| dc.date.available | 2022-11-24T03:08:36Z | - |
| dc.date.copyright | 2021-11-02 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-10-27 | |
| dc.identifier.citation | (1) Bogen, G.; Kim, J. S.; Bakland, L. K. Direct Pulp Capping With Mineral Trioxide Aggregate: An Observational Study. The Journal of the American Dental Association 2008, 139, 305-315. (2) Willershausen, B.; Willershausen, I.; Ross, A.; Velikonja, S.; Kasaj, A.; Blettner, M. Retrospective study on direct pulp capping with calcium hydroxide. Quintessence Int 2011, 42, 165-171. (3) Tawil, P. Z.; Duggan, D. J.; Galicia, J. C. Mineral trioxide aggregate (MTA): its history, composition, and clinical applications. Compend Contin Educ Dent 2015, 36, 247-252; quiz 254, 264. (4) Hilton, T. J. Keys to clinical success with pulp capping: a review of the literature. Oper Dent 2009, 34, 615-625. (5) Torabinejad, M.; Hong, C. U.; McDonald, F.; Pitt Ford, T. R. Physical and chemical properties of a new root-end filling material. J Endod 1995, 21, 349-353. (6) Aeinehchi, M.; Eslami, B.; Ghanbariha, M.; Saffar, A. S. Mineral trioxide aggregate (MTA) and calcium hydroxide as pulp-capping agents in human teeth: a preliminary report. Int Endod J 2003, 36, 225-231. (7) Hilton, T. J.; Ferracane, J. L.; Mancl, L. Comparison of CaOH with MTA for direct pulp capping: a PBRN randomized clinical trial. J Dent Res 2013, 92, 16s-22s. (8) Malkondu, Ö.; Kazandağ, M. K.; Kazazoğlu, E. A Review on Biodentine, a Contemporary Dentine Replacement and Repair Material. BioMed Research International 2014, 2014, 160951. (9) Arandi, N. Z.; Rabi, T. TheraCal LC: From Biochemical and Bioactive Properties to Clinical Applications. Int J Dent 2018, 2018, 3484653. (10) Suh B. I., Y. R., Cannon M., Martin D. . Polymerizable dental pulp healing, capping, and lining material and method for use. International Patent 2008, 1. (11) Gandolfi, M. G.; Siboni, F.; Prati, C. Chemical-physical properties of TheraCal, a novel light-curable MTA-like material for pulp capping. Int Endod J 2012, 45, 571-579. (12) Jeanneau, C.; Laurent, P.; Rombouts, C.; Giraud, T.; About, I. Light-cured Tricalcium Silicate Toxicity to the Dental Pulp. J Endod 2017, 43, 2074-2080. (13) Chohayeb, A. A.; Adrian, J. C.; Salamat, K. Pulpal response to tricalcium phosphate as a capping agent. Oral Surg Oral Med Oral Pathol 1991, 71, 343-345. (14) Guerrero-Gironés, J.; Alcaina-Lorente, A.; Ortiz-Ruiz, C.; Ortiz-Ruiz, E.; Pecci-Lloret, M. P.; Ortiz-Ruiz, A. J.; Rodríguez-Lozano, F. J.; Pecci-Lloret, M. R. Biocompatibility of a HA/β-TCP/C Scaffold as a Pulp-Capping Agent for Vital Pulp Treatment: An In Vivo Study in Rat Molars. International Journal of Environmental Research and Public Health 2021, 18, 3936. (15) Chang, M. C.; Lin, L. D.; Wu, M. T.; Chan, C. P.; Chang, H. H.; Lee, M. S.; Sun, T. Y.; Jeng, P. Y.; Yeung, S. Y.; Lin, H. J.; Jeng, J. H. Effects of Camphorquinone on Cytotoxicity, Cell Cycle Regulation and Prostaglandin E2 Production of Dental Pulp Cells: Role of ROS, ATM/Chk2, MEK/ERK and Hemeoxygenase-1. PLoS One 2015, 10, e0143663. (16) Leo Zack, G. C. Pulp response to externally applied heat. O.S., O.M. O.P. 1965, 19, 515-530. (17) Majeed, A.; AlShwaimi, E. Push-Out Bond Strength and Surface Microhardness of Calcium Silicate-Based Biomaterials: An in vitro Study. Med Princ Pract 2017, 26, 139-145. (18) Chun, K.; Choi, H.; Lee, J.-Y. Comparison of mechanical property and role between enamel and dentin in the human teeth. Journal of dental biomechanics 2014, 5, 1758736014520809. (19) Elnaghy, A. M. Influence of Acidic Environment on Properties of Biodentine and White Mineral Trioxide Aggregate: A Comparative Study. Journal of Endodontics 2014, 40, 953-957. (20) Stejskal, J.; Riede, A.; Hlavatá, D.; Prokeš, J.; Helmstedt, M.; Holler, P. The effect of polymerization temperature on molecular weight, crystallinity, and electrical conductivity of polyaniline. Synthetic Metals 1998, 96, 55-61. (21) P., G. The controversy: restoration technique – should there be exclusively adhesive restorations? Dtsch Zahnӓrztl Z 2005, 60, 11-14. (22) Jung, J.-Y.; Woo, S.-M.; Lee, B.-N.; Koh, J.-T.; Nör, J. E.; Hwang, Y.-C. Effect of Biodentine and Bioaggregate on odontoblastic differentiation via mitogen-activated protein kinase pathway in human dental pulp cells. International Endodontic Journal 2015, 48, 177-184. (23) Nowicka, A.; Lipski, M.; Parafiniuk, M.; Sporniak-Tutak, K.; Lichota, D.; Kosierkiewicz, A.; Kaczmarek, W.; Buczkowska-Radlińska, J. Response of Human Dental Pulp Capped with Biodentine and Mineral Trioxide Aggregate. Journal of Endodontics 2013, 39, 743-747. (24) Jean, A.; Pouezat, J.; Daculsi, G. Pulpal Response to Calcium Phosphate Materials. In Vivo Study of Calcium Phosphate Materials in Endodontics. Cells and materials 1993, 3, 7. (25) Watts, A.; Paterson, R. C. A comparison of pulp responses to two different materials in the dog and the rat. Oral Surgery, Oral Medicine, Oral Pathology 1981, 52, 648-652. (26) H., N. Healing processes in the pulp on capping; a morphologic study; experiments on surgical lesions of the pulp in dog and man. Acta Odontol Scand 1955, 13, 1-130. (27) Stashenko, P.; Wang, C.-Y.; Tani-Ishii, N.; Yu, S. M. Pathogenesis of induced rat periapical lesions. Oral Surgery, Oral Medicine, Oral Pathology 1994, 78, 494-502. (28) M, B. Dentin and Pulp in Restorative Dentistry. Nacka: Dental Therapeutics 1981, 81. (29) Simon, S.; Cooper, P.; Smith, A.; Picard, B.; Naulin Ifi, C.; Berdal, A. Evaluation of a new laboratory model for pulp healing: preliminary study. International Endodontic Journal 2008, 41, 781-790. (30) Song, M.; Kim, S.; Kim, T.; Park, S.; Shin, K.-H.; Kang, M.; Park, N.-H.; Kim, R. Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice. J Vis Exp 2017, 54973. (31) Dammaschke, T. Rat molar teeth as a study model for direct pulp capping research in dentistry. Laboratory Animals 2010, 44, 1-6. (32) Sabir, A.; Tabbu, C. R.; Agustiono, P.; Sosroseno, W. Histological analysis of rat dental pulp tissue capped with propolis. Journal of Oral Science 2005, 47, 135-138. (33) Goldberg, M.; Smith, A. J. Cells and Extracellular Matrices of Dentin and Pulp: A Biological Basis for Repair and Tissue Engineering. Critical Reviews in Oral Biology Medicine 2004, 15, 13-27. (34) Chicarelli, L. P. G.; Webber, M. B. F.; Amorim, J. P. A.; Rangel, A. L. C. A.; Camilotti, V.; Sinhoreti, M. A. C.; Mendonça, M. J. Effect of Tricalcium Silicate on Direct Pulp Capping: Experimental Study in Rats. Eur J Dent 2021, 15, 101-108. (35) Liu, Q.; Ma, Y.; Wang, J.; Zhu, X.; Yang, Y.; Mei, Y. Demineralized bone matrix used for direct pulp capping in rats. PLOS ONE 2017, 12, e0172693. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/80527 | - |
| dc.description.abstract | 近年來牙科活髓治療越趨普及，在牙髓暴露齲齒處理的治療選擇除了根管治療之外還有其他可能性。活髓治療原本較常使用於乳牙齒列，但隨著近年來材料的發展，在成人齒列之成功率也提高了許多。儘管活髓治療之成功率和牙齒本身之初始狀況有最大的相關，但使用的活髓治療材料也有很大的影響，近年來較常使用成功率較高的活髓治療材料有mineral trioxide aggregate (MTA)及生物陶瓷材料像是Biodentine，但現有這些高成功率材料仍有些缺點，像是臨床使用操控性、較長的固化時間、較高的價格及牙齒變色等，而另外一款光聚合式材料Theracal LC解決了長固化時間的問題，但其細胞毒性仍較高不適合作為活髓治療使用。故本實驗目標為評估不同比例混合之低毒性光聚合樹脂材料及填料作為直接活髓治療材料之可行性。 本實驗選用兩種不同的樹脂材料並分為兩大組，A大組使用之樹脂材料為ethoxylated (15) trimethylolpropane triacrylate，並混入不同比例之tricalcium phosphate (TCP)及hydroxyapatite (HA)(分為A0-A5組)，B大組使用之樹脂材料為polyethylene glycol (600) diacrylate，一樣混入不同比例之TCP及HA(分為B1-B5組)。硬度測試(每組n=6)及固化放熱測試(每組n=5)在材料上進行。細胞活性測試及分化測試(Alizarin Red Staining (ARS) assay)利用人類牙髓細胞做體外實驗(每組n=6)，最後再利用B4及B5組別與Biodentine及control組於SD大鼠(Sprague-Dawley rats)之上顎大臼齒做體內實驗，並切片染色觀察。 實驗結果發現硬度部分各組結果均介於4至7 HV之間，和現行市售活髓治療材料MTA或Biodentine相比顯著低許多；而聚合時溫度上升量在B4及B5組(有加入較高比例之去離子水)皆少於5度C，其餘光聚合材料組別均上升超過11度C，有60%可能性造成牙髓之不可逆損傷；牙髓細胞活性測試部分僅第1天之B4組顯著低於B5組及control組，第3天、第7天各組間皆無顯著差異；牙髓細胞分化實驗部分在未加分化配方的部分到了第10天時B5及Biodentine組顯著高於B4及control組，相較於第4、7天也有顯著較高；大鼠體內實驗部分可以在第三週及第六週之Biodentine及B5組別觀察到較明顯之礦化生成，Biodentine組在第六週時無觀察到發炎情況，B5組則是在第三週及第六週皆無發現發炎情況。 由本實驗結論可知，本實驗材料因硬度不高，外層仍需高抗磨耗高硬度之材料進行窩洞復形。B4及B5材料之溫度上升量在安全範圍內，不用擔心牙髓產生不可逆損傷。而B5材料對於牙髓細胞幾乎無毒性，且發炎反應結束時間相較於Biodentine更快，也和Biodentine一樣可以誘導牙髓組織產生礦化，而其相較於Biodentine之優點為臨床使用時有更快的固化時間。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-24T03:08:36Z (GMT). No. of bitstreams: 1 U0001-2610202116514000.pdf: 4229894 bytes, checksum: b754a98c048c33f6a83ab42914d6e18d (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 中文摘要 i Abstract iii 目錄 v 圖目錄 vii 表目錄 viii 第一章 文獻回顧 1 1.1 活髓治療技術 1 1.1.1 活髓治療(Vital pulp therapy)臨床流程 1 1.2 目前臨床使用之覆髓材料應用於活髓治療之可行性 1 1.2.1 Mineral Trioxide Aggregate (MTA) 1 1.2.2 Biodentine 2 1.2.3 氫氧化鈣(Calcium Hydroxide) 3 1.2.4 TheraCal LC 3 1.3 材料選用回顧 4 1.3.1 基底材料 4 1.3.2 光聚合樹脂材料 4 1.4 材料生物相容性測試 5 1.4.1 體外實驗 5 1.4.2 體內實驗 5 第二章 研究動機與目的 6 2.1 研究動機 6 2.2 研究目的 6 第三章 實驗材料及方法 7 3.1 使用材料及混和方法 7 3.1.1 材料選擇 7 3.1.2 混合方法 10 3.2 材料硬度測試 12 3.3 固化放熱測試 13 3.4體外實驗-牙髓幹細胞活性測試 15 3.4.1 細胞培養 15 3.4.2 conditioned medium配製 15 3.4.3 MTT assay 實驗步驟 16 3.5 體外實驗-牙髓幹細胞分化測試 17 3.5.1 分化培養基調配 17 3.5.2 ARS assay實驗步驟 17 3.6 體內實驗-大鼠活髓治療測試 18 3.7 統計分析 21 第四章 實驗結果 22 4.1 材料硬度測試結果 22 4.2 固化放熱測試結果 23 4.3 體外實驗-牙髓幹細胞活性測試結果 25 4.4 體外實驗-牙髓幹細胞分化測試結果 26 4.5 體內實驗-大鼠活髓治療測試結果 35 第五章 討論 38 5.1 材料物理性質影響 38 5.2 溫度影響 39 5.3 生物相容性 40 第六章 結論與未來研究方向 45 參考文獻 47 | |
| dc.language.iso | zh-TW | |
| dc.subject | 活髓治療材料 | zh_TW |
| dc.subject | 活髓治療 | zh_TW |
| dc.subject | 光聚合樹脂活髓治療材料 | zh_TW |
| dc.subject | Vital pulp therapy | en |
| dc.subject | Vital pulp therapy material | en |
| dc.subject | Light-cured resinous vital pulp therapy material | en |
| dc.title | 磷酸三鈣及羥機磷灰石基底之光聚合式活髓治療生物材料之發展 | zh_TW |
| dc.title | Development of tricalcium phosphate (TCP) and hydroxyapatite (HA) based light-cured biomaterial for vital pulp therapy | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.advisor-orcid | 陳敏慧(0000-0002-4853-1906) | |
| dc.contributor.oralexamcommittee | 陳容慈(Hsin-Tsai Liu),黃仁勇(Chih-Yang Tseng) | |
| dc.subject.keyword | 活髓治療,活髓治療材料,光聚合樹脂活髓治療材料, | zh_TW |
| dc.subject.keyword | Vital pulp therapy,Vital pulp therapy material,Light-cured resinous vital pulp therapy material, | en |
| dc.relation.page | 49 | |
| dc.identifier.doi | 10.6342/NTU202104249 | |
| dc.rights.note | 同意授權(限校園內公開) | |
| dc.date.accepted | 2021-10-27 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
| 顯示於系所單位： | 臨床牙醫學研究所 | |
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