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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鄧述諄(Shu-Chun Teng) | |
| dc.contributor.author | Pei-Heng Jiang | en |
| dc.contributor.author | 姜沛恆 | zh_TW |
| dc.date.accessioned | 2022-11-24T03:06:58Z | - |
| dc.date.available | 2021-12-30 | |
| dc.date.available | 2022-11-24T03:06:58Z | - |
| dc.date.copyright | 2021-12-30 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-12-05 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/80453 | - |
| dc.description.abstract | 老化是種生理功能逐漸喪失的複雜現象。為了探討老化相關的分子機制，我們先以卡路里限制的生長條件培養酵母菌，這是一種行之有效的延長壽命的方法，之後進行定量質譜篩選，並偵測到酵母蛋白中對卡路里限制有顯著反應的所有磷酸化/去磷酸化位點。功能篩選發現Ids2在CR下被PP2C活化，在葡萄糖攝入時被PKA失活。營養過度消耗會損害HSP90-Ids2複合物的折疊活性，表現出熱敏感性、粒線體缺陷和壽命縮短。我進一步證明了Ids2作為維持粒線體DNA和功能必不可少的輔助蛋白。比對Hsc82物理相互作用蛋白、粒線體組成蛋白和具有呼吸缺陷的突變體基因這三個數據庫後，我篩選出Atp3，一個complex V ATP合酶的組成蛋白為 Ids2 的調控對象。IDS2的缺失使Atp3不穩定，Ids2中間區域的α-螺旋結合Atp3後進行折疊修飾。Ids2或Atp3的短缺導致粒線體DNA的丟失。膜間隙蛋白酶Yme1對維持Atp3蛋白量上扮演重要的角色。除此之外，細胞進行呼吸作用時，Ids2 表現量會大幅上升。總結上來說，本研究首次發現Ids2的折疊修飾對象，並解釋了Ids2的缺乏如何造成粒線體DNA丟失和粒線體功能障礙。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-24T03:06:58Z (GMT). No. of bitstreams: 1 U0001-0312202115221200.pdf: 9488054 bytes, checksum: 5b27a0fb3f12ccb0a5c1c63cabd3442a (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 口試委員審定書 i 致謝 ii 聲明 iii 中文摘要 iv Abstract v Introduction 1 Results 7 Discussion 17 Methods 21 Figures and Figure Legends 28 Tables 59 References 79 | |
| dc.language.iso | en | |
| dc.subject | 分子伴侶蛋白 | zh_TW |
| dc.subject | 蛋白質恆定 | zh_TW |
| dc.subject | 老化 | zh_TW |
| dc.subject | 粒線體 | zh_TW |
| dc.subject | Ids2 | en |
| dc.subject | Proteostasis | en |
| dc.subject | ATP synthase | en |
| dc.subject | Aging | en |
| dc.subject | Mitochondria | en |
| dc.title | HSP90輔助摺疊蛋白Ids2具維持粒線體DNA及ATP合酶穩定性之功能 | zh_TW |
| dc.title | An HSP90 cochaperone Ids2 maintains the stability of mitochondrial DNA and ATP synthase | en |
| dc.date.schoolyear | 110-1 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 林敬哲(Hsin-Tsai Liu),張壯榮(Chih-Yang Tseng),吳青錫,劉雅雯 | |
| dc.subject.keyword | 老化,蛋白質恆定,粒線體,分子伴侶蛋白, | zh_TW |
| dc.subject.keyword | Aging,Proteostasis,Mitochondria,Ids2,ATP synthase, | en |
| dc.relation.page | 107 | |
| dc.identifier.doi | 10.6342/NTU202104511 | |
| dc.rights.note | 同意授權(限校園內公開) | |
| dc.date.accepted | 2021-12-06 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 微生物學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
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