請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/8013
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 馮哲川(Zhe-Chuan Feng) | |
dc.contributor.author | Shou-Hao Wu | en |
dc.contributor.author | 吳守浩 | zh_TW |
dc.date.accessioned | 2021-05-19T18:02:44Z | - |
dc.date.available | 2024-08-04 | |
dc.date.available | 2021-05-19T18:02:44Z | - |
dc.date.copyright | 2014-09-04 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-08-05 | |
dc.identifier.citation | [1] D.-S. Kim, J.-E. Park, J.-K. Shin, P. K. Kim, G. Lim, and S. Shoji, 'An extended gate FET-based biosensor integrated with a Si microfluidic channel for detection of protein complexes,' Sensors and Actuators B: Chemical, vol. 117, pp. 488-494, 2006.
[2] P. O. Brown and D. Botstein, 'Exploring the new world of the genome with DNA microarrays,' Nature genetics, vol. 21, pp. 33-37, 1999. [3] K. E. Nelson, L. Gamble, L. S. Jung, M. S. Boeckl, E. Naeemi, S. L. Golledge, et al., 'Surface characterization of mixed self-assembled monolayers designed for streptavidin immobilization,' Langmuir, vol. 17, pp. 2807-2816, 2001. [4] P. I. Reyes, C.-J. Ku, Z. Duan, Y. Lu, A. Solanki, and K.-B. Lee, 'ZnO thin film transistor immunosensor with high sensitivity and selectivity,' Applied Physics Letters, vol. 98, p. 173702, 2011. [5] C. V. Sapan, R. L. Lundblad, and N. C. Price, 'Colorimetric protein assay techniques,' Biotechnology and applied Biochemistry, vol. 29, pp. 99-108, 1999. [6] R. L. Ashley, J. Militoni, F. Lee, A. Nahmias, and L. Corey, 'Comparison of Western blot (immunoblot) and glycoprotein G-specific immunodot enzyme assay for detecting antibodies to herpes simplex virus types 1 and 2 in human sera,' Journal of Clinical Microbiology, vol. 26, pp. 662-667, 1988. [7] M. S. Smeltzer, M. E. Hart, and J. J. Iandolo, 'Quantitative spectrophotometric assay for staphylococcal lipase,' Applied and environmental microbiology, vol. 58, pp. 2815-2819, 1992. [8] E. Stenberg, B. Persson, H. Roos, and C. Urbaniczky, 'Quantitative determination of surface concentration of protein with surface plasmon resonance using radiolabeled proteins,' Journal of colloid and interface science, vol. 143, pp. 513-526, 1991. [9] B.-K. Sohn and C.-S. Kim, 'A new pH-ISFET based dissolved oxygen sensor by employing electrolysis of oxygen,' Sensors and Actuators B: Chemical, vol. 34, pp. 435-440, 1996. [10] S. V. Dzyadevich, Y. I. Korpan, V. N. Arkhipova, M. Y. Alesina, C. Martelet, A. V. El’Skaya, et al., 'Application of enzyme field-effect transistors for determination of glucose concentrations in blood serum,' Biosensors and bioelectronics, vol. 14, pp. 283-287, 1999. [11] B. L. Allen, P. D. Kichambare, and A. Star, 'Carbon Nanotube Field‐Effect‐Transistor‐Based Biosensors,' Advanced Materials, vol. 19, pp. 1439-1451, 2007. [12] I. Lauks, P. Chan, and D. Babic, 'The extended gate chemically sensitive field effect transistor as multi-species microprobe,' Sensors and Actuators, vol. 4, pp. 291-298, 1983. [13] L.-T. Yin, J.-C. Chou, W.-Y. Chung, T.-P. Sun, and S.-K. Hsiung, 'Study of indium tin oxide thin film for separative extended gate ISFET,' Materials Chemistry and Physics, vol. 70, pp. 12-16, 2001. [14] J.-C. Chen, J.-C. Chou, T.-P. Sun, and S.-K. Hsiung, 'Portable urea biosensor based on the extended-gate field effect transistor,' Sensors and Actuators B: Chemical, vol. 91, pp. 180-186, 2003. [15] A. Manz, N. Graber, and H. Widmer, 'Miniaturized total chemical analysis systems: a novel concept for chemical sensing,' Sensors and actuators B: Chemical, vol. 1, pp. 244-248, 1990. [16] S. Sharma, K. Buchholz, S. M. Luber, U. Rant, M. Tornow, and G. Abstreiter, 'Silicon-on-insulator microfluidic device with monolithic sensor integration for μTAS applications,' Microelectromechanical Systems, Journal of, vol. 15, pp. 308-313, 2006. [17] S. C. Jacobson, R. Hergenroder, L. B. Koutny, and J. M. Ramsey, 'High-speed separations on a microchip,' Analytical Chemistry, vol. 66, pp. 1114-1118, 1994. [18] M. Szumski and B. Buszewski, 'State of the art in miniaturized separation techniques,' Critical reviews in analytical chemistry, vol. 32, pp. 1-46, 2002. [19] S. C. Jakeway, A. J. de Mello, and E. L. Russell, 'Miniaturized total analysis systems for biological analysis,' Fresenius' journal of analytical chemistry, vol. 366, pp. 525-539, 2000. [20] P.-A. Auroux, D. Iossifidis, D. R. Reyes, and A. Manz, 'Micro total analysis systems. 2. Analytical standard operations and applications,' Analytical chemistry, vol. 74, pp. 2637-2652, 2002. [21] D. Erickson and D. Li, 'Integrated microfluidic devices,' Analytica Chimica Acta, vol. 507, pp. 11-26, 2004. [22] M. J. de Boer, R. W. Tjerkstra, J. Berenschot, H. V. Jansen, G. Burger, J. Gardeniers, et al., 'Micromachining of buried micro channels in silicon,' Microelectromechanical Systems, Journal of, vol. 9, pp. 94-103, 2000. [23] L.-M. Fu, R.-J. Yang, C.-H. Lin, Y.-J. Pan, and G.-B. Lee, 'Electrokinetically driven micro flow cytometers with integrated fiber optics for on-line cell/particle detection,' Analytica Chimica Acta, vol. 507, pp. 163-169, 2004. [24] K. Hatakeyama, T. Tanaka, M. Sawaguchi, A. Iwadate, Y. Mizutani, K. Sasaki, et al., 'Microfluidic device using chemiluminescence and a DNA-arrayed thin film transistor photosensor for single nucleotide polymorphism genotyping of PCR amplicons from whole blood,' Lab on a Chip, vol. 9, pp. 1052-1058, 2009. [25] C. A. Janeway, P. Travers, M. Walport, and M. J. Shlomchik, 'The structure of a typical antibody molecule,' 2001. [26] 鄭. Reist, 劉希平, '微粒導論,' ed: 國立編譯館, 2001. [27] P. J. H. P.G. Gray, S.H. Lewis , R.G. Meyer, Analysis and Design of Analog Integrated circuit, 4th edition: John Wiley & Sons ,Inc., 2001. [28] W. F. Smith, Foundations of Materials Science and Engineering 3rd ed.: McGraw-Hill, 2004. [29] J. C. Young, V. R. Agashe, K. Siegers, and F. U. Hartl, 'Pathways of chaperone-mediated protein folding in the cytosol,' Nature reviews Molecular cell biology, vol. 5, pp. 781-791, 2004. [30] K. A. Dill, 'Dominant forces in protein folding,' Biochemistry, vol. 29, pp. 7133-7155, 1990. [31] A. C. Wallace, R. A. Laskowski, and J. M. Thornton, 'LIGPLOT: a program to generate schematic diagrams of protein-ligand interactions,' Protein engineering, vol. 8, pp. 127-134, 1995. [32] A. Ho and T. C. Sudhof, 'Binding of F-spondin to amyloid-β precursor protein: a candidate amyloid-β precursor protein ligand that modulates amyloid-β precursor protein cleavage,' Proceedings of the National Academy of Sciences of the United States of America, vol. 101, pp. 2548-2553, 2004. [33] U. Francke, M. S. Brown, and J. L. Goldstein, 'Assignment of the human gene for the low density lipoprotein receptor to chromosome 19: synteny of a receptor, a ligand, and a genetic disease,' Proceedings of the National Academy of Sciences, vol. 81, pp. 2826-2830, 1984. [34] G. Jones, P. Willett, and R. C. Glen, 'Molecular recognition of receptor sites using a genetic algorithm with a description of desolvation,' Journal of molecular biology, vol. 245, pp. 43-53, 1995. [35] R. B. Sekar and A. Periasamy, 'Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations,' The Journal of cell biology, vol. 160, pp. 629-633, 2003. [36] I. L. Medintz, H. T. Uyeda, E. R. Goldman, and H. Mattoussi, 'Quantum dot bioconjugates for imaging, labelling and sensing,' Nature materials, vol. 4, pp. 435-446, 2005. [37] Q. A. Pankhurst, J. Connolly, S. Jones, and J. Dobson, 'Applications of magnetic nanoparticles in biomedicine,' Journal of physics D: Applied physics, vol. 36, p. R167, 2003. [38] Y. C. Cao, R. Jin, J.-M. Nam, C. S. Thaxton, and C. A. Mirkin, 'Raman dye-labeled nanoparticle probes for proteins,' Journal of the American Chemical Society, vol. 125, pp. 14676-14677, 2003. [39] J. Holbrook and R. Wolfe, 'Malate dehydrogenase. X. Fluorescence microtitration studies of D-malate, hydroxymalonate, nicotinamide dinucleotide, and dihydronicotinamide-adenine dinucleotide binding by mitochondrial and supernatant porcine heart enzymes,' Biochemistry, vol. 11, pp. 2499-2502, 1972. [40] C. Thorne, L. Grossman, and N. Kaplan, 'Starch-gel electrophoresis of malate dehydrogenase,' Biochimica et Biophysica Acta (BBA)-Specialized Section on Enzymological Subjects, vol. 73, pp. 193-203, 1963. [41] Y. R. Gokarn, R. M. Fesinmeyer, A. Saluja, V. Razinkov, S. F. Chase, T. M. Laue, et al., 'Effective charge measurements reveal selective and preferential accumulation of anions, but not cations, at the protein surface in dilute salt solutions,' Protein Science, vol. 20, pp. 580-587, 2011. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/8013 | - |
dc.description.abstract | 論文中包含了兩個部分。首先是將薄膜電晶體應用於血清蛋白的偵測,其次是其在偵測蛋白質與配體之間的反應行為。
生物分子的偵測是當今熱門的研究。我們設計的蛋白質感測器結合了微流道系統。當待測物經由微流道擴散到金感測板時,會造成薄膜電晶體電流瞬間增加。不同的流道長度或是蛋白質濃度,都會影響電流變化的時間以及感應電荷的數量。而這些結果又可以經由理論關係式相互推導,因而達到偵測與量化的目的。我們的設計可以得到電性方面的資訊(蛋白質帶電量),並且同時可以得到流體的訊息(和蛋白質的尺寸大小及質量相關),是一個非常簡易卻又方便迅速的蛋白質感測器。 另外,蛋白質與配體間的交互作用一直是世界各大藥廠相當關切的議題。我們首先用薄膜電晶體感測器偵測蛋白質與配體分別的訊號,並給出一個估算式以預估量測未知擴散係數的生物分子時適合的調配濃度。我們進行螢光染色的影像拍攝以驗證先前純粹電性量測的結果。最後我們將配體引入蛋白質溶液並量測其電性反應,以探究兩者之間的反應行為。 | zh_TW |
dc.description.abstract | There are two parts in this thesis. First of all, a TFT-based protein sensor with a microfluidic channel is designed to detect and quantify the bio-molecules. Secondly, the TFT sensor is further applied to detect the protein-ligand interaction between MDH and NADH.
We demonstrate a TFT-based protein sensor combined with microfluidic channel. Due to the concentration gradient in solution, the analyte diffuses to the sensing pad through the microfluidic channel and causes a drain current increment. With various microfluidic channel lengths or different concentrations of IgG antibody, the results of response (diffusion) time and the amount of induced charges are quite distinguishable. All results following some theoretical relations. Thus, the unknown concentrations of IgG antibody can be obtained. Our design can acquire not only electrical information (the charges carried by the bio-molecules) but also fluidic information (diffusion time which is related to the size and weight of the bio-molecules). We next examine the diffusion and electrical signals of protein, ligand, and protein-ligand interaction. They were first measured separately to obtain information that would be referenced for later experiment. An estimation equation is provided for new analyte with unknown diffusion coefficient, and is verified in our experiment to have accurate evaluation of diffusion time. We also did the fluorescent snapshots to gain a visual picture of the diffusion scheme aside from electrical measurement. Finally, protein-ligand interaction is detected by our TFT sensor. | en |
dc.description.provenance | Made available in DSpace on 2021-05-19T18:02:44Z (GMT). No. of bitstreams: 1 ntu-103-R01941053-1.pdf: 1562260 bytes, checksum: 707651616da35cebd761b892a756c38e (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 謝誌 I
Abstract II 摘要 IV List of Figures VII List of Tables VIII Pages Chapter 1 Introduction 1 1.1 Background of protein bio-sensor 1 1.1.1 Overview of protein detection 1 1.1.2 FET-based biosensors 3 Chapter 2 TFT-based Protein Sensor with Microfluidic Channels 7 2.1 Introduction 7 2.1.1 Overview of the microfluidic system 7 2.1.2 Introduction of the IgG antibody 10 2.1.3 Structure and mechanism 11 2.2 Device fabrication and measurement 13 2.2.1 Fabrication 13 2.2.2 Method of measurement 16 2.3 Characterizations of TFT-based protein sensors 17 2.3.1 Drain current variations in time domain 17 2.3.2 Induced charge variations in time domain 22 2.4 Summary 28 Chapter 3 TFT-based Biosensors and Protein-ligand Interaction 30 3.1 Introduction 30 3.1.1 Overview of Protein-ligand interaction 30 3.1.2 Introduction of MDH and NADH 33 3.2 Device fabrication and measurement 34 3.2.1 Fabrication 34 3.2.2 Method of measurement 36 3.3 Results and Discussion 37 3.4 Summary 45 Chapter 4 Conclusion 47 4.1 TFT-based biosensors with microfluidic channels 47 4.2 TFT-based biosensors and protein-ligand interaction 48 References 49 List of Figures Fig 1.1 Demonstration of nanorods transistor-based biosensor 4 Fig 1.2 Illustration of signal of protein detection process 4 Fig 1.3 Cross section of an EGFET bio-sensor 5 Fig 2.1 The structure of microfluidic network 8 Fig 2.2 Micro-channel is used for delivering the solutions 9 Fig 2.3 Structure of TFT-based biosensor with microfluidic channel 11 Fig 2.4 Fabrication process 14 Fig 2.5 Fabrication of 100 μm SU8-2100 15 Fig 2.6 Solution condition before measurement 16 Fig 2.7 (2700μm microfluidic channel) Drain current and induced charge variation 17 Fig 2.8 Properties of a-IGZO TFT at t = 0 and 15 min 19 Fig 2.9 (1800μm microfluidic channel) Drain current and induced charge variation 20 Fig 2.10 Summary of response time and △Qmax (IgG antibody : PBS= 1:50) 21 Fig 2.11 Summary of response time and △Qmax 24 Fig 2.12 Summary of response time and △Qmax (2700 μm Microchannel length) 27 Fig 2.13 Summary of response time and △Qmax (1800 μm Microchannel length) 27 Fig 3.1. Fabrication process 35 Fig 3.2. Drain current sampling of NADH sensing 37 Fig 3.3. Transfer curve of NADH sensing 38 Fig 3.4. Drain current sampling and transfer curve of MDH sensing 41 Fig 3.5. Snapshots of the diffusion process of NHS-fluorescent-binding MDH 43 Fig 3.6. Drain current variation of mixing analyte diffusion in time domain 44 List of Tables Table 2.1 Summary of response time and △Qmax 23 Table 2.2 Diffusion coefficient calculated from equation (2.1) 24 Table 2.3 ΔQReal and ΔQCalculated from relation (2.6) 26 | |
dc.language.iso | en | |
dc.title | 以結合微流道之銦鎵鋅氧化物薄膜電晶體偵測蛋白質與配體之反應行為 | zh_TW |
dc.title | Integration of Microfluidic Channels with IGZO Thin Film Transistors for Detecting Protein-Ligand Interactions | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 黃建璋(JianJang Huang) | |
dc.contributor.oralexamcommittee | 楊宗霖(Tsung-Lin Yang),吳育任(Yuh-Renn Wu) | |
dc.subject.keyword | 非晶相銦鎵鋅氧化物,薄膜電晶體,生物感測器,微流道,蛋白質配體反應行為, | zh_TW |
dc.subject.keyword | a-IGZO,TFT,protein sensor,microfluidic channel,protein-ligand interaction, | en |
dc.relation.page | 52 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2014-08-05 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 光電工程學研究所 | zh_TW |
dc.date.embargo-lift | 2024-08-04 | - |
顯示於系所單位: | 光電工程學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-103-1.pdf 此日期後於網路公開 2024-08-04 | 1.53 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。