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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 于昌平(Chang-Ping Yu) | |
| dc.contributor.author | Chien-Yun Tseng | en |
| dc.contributor.author | 曾千芸 | zh_TW |
| dc.date.accessioned | 2022-11-23T09:25:37Z | - |
| dc.date.available | 2023-06-16 | |
| dc.date.available | 2022-11-23T09:25:37Z | - |
| dc.date.copyright | 2021-08-04 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-07-12 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/80091 | - |
| dc.description.abstract | "近年來塑膠產業興盛，為了工業需求時常需添加塑化劑增加塑膠的柔軟度，其中，鄰苯二甲酸二(2-乙基己基)酯 (Di(2-ethylhexyl) phthalate, DEHP ) 為世界上使用較大量的塑化劑，因結構中含有苯環及較長碳鏈造成其性質安定不易分解，故廣泛存在於環境中且易累積造成生物毒性，近年來處理被DEHP污染的場域便是當務之急。其中以生物降解DEHP的方式較為有效、成本低且對環境友善，而當中能夠快速降解DEHP之菌種需特別由環境中分離純化，在利用微生物降解污染物上，如何保存菌種以及維持其活性便是很重要的環節。 本研究主題為將具有降解DEHP能力之菌種保存以及固定於以靜電紡絲技術製作出之奈米纖維膜中，此兩株菌分別為能將DEHP分解成中間產物鄰苯二甲酸 (phthalate acid, PA)的Acidovorax sp.，以及將後續PA降解為無毒最終產物的Thauera sp.，在 4℃下保存於纖維中數天後再進行後續降解水中DEHP之試驗。因靜電紡絲之纖維具有高比表面積及材料多樣等特性，故須先進行纖維材料優化，經共軸及單軸進行方法可行性評估後，發現共軸之穩定性較低，無法長時間收集纖維達到一定菌量，較不利於應用，而非水溶性纖維其溶劑通常對微生物有毒性，無法維持其活性，而以單軸形式製作出水溶性纖維較適合固定及保存菌種來降解DEHP，因此進行電紡製作出聚乙烯醇 (Polyvinyl alcohol, PVA) 纖維。 為了確認菌種經過電紡以及在纖維保存數天後是否仍具有降解DEHP之能力，本實驗設計不同保存天數再將纖維溶解於無氧培養基中以HPLC檢測水中DEHP及中間產物PA之濃度。由實驗結果可知，兩株菌種經電紡後保存於PVA纖維中21天內能將水中至少 90 %之1 mM DEHP在無氧狀態下完全降解，因DEHP為塑化劑，會被水中PVA進行吸附，但菌種仍能代謝吸附於高分子中的DEHP，且因菌種均為脫硝菌，同時也能將10 mM之硝酸根去除至少50 %以上。最後由共軛焦螢光顯微鏡顯示菌種確實被包覆於纖維內部，綜觀以上結果可得出本研究之優勢為：(1)以纖維保存菌種在存放以及運送上相比於液態均較為方便。(2)纖維中可存放大量菌種，於水中能快速溶解將菌種釋出且不需事先活化，能夠有效降解DEHP。 (3)可將水中硝酸根去除變為對環境較無害之氮氣。(4) PVA為環境友善之高分子，於環境中可被自然分解。 " | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-23T09:25:37Z (GMT). No. of bitstreams: 1 U0001-0907202118530500.pdf: 4176889 bytes, checksum: da4eb4f0cd50eb06760c7e44ca6e40a0 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | "口試委員會審定書 II 致謝 III 摘要 IV Abstract VI 圖目錄 XII 表目錄 XIV 第一章 緒論 1 1.1 研究背景 1 1.2 研究動機 2 第二章 文獻回顧 3 2.1 鄰苯二甲酸二(2-乙基己基)酯(DEHP)簡介 3 2.1.1 DEHP物化特性 3 2.1.2 DEHP環境流佈 5 2.1.3 DEHP生物毒性 7 2.2 微生物對DEHP的降解 8 2.2.1 DEHP降解途徑 8 2.2.2 微生物種類 10 2.3 靜電紡絲 12 2.3.1 基本原理 12 2.3.2 基本裝置 12 2.3.3 影響因素 15 2.3.4 聚乙烯醇 (Polyvinyl alcohol, PVA) 17 2.4 靜電紡絲應用於細胞固定化之研究 17 2.5 微生物保存方法 22 第三章 材料與方法 25 3.1. 實驗藥品與設備 25 3.1.1. 實驗藥品 25 3.1.2. 實驗設備 26 3.2. 實驗架構 27 3.3. 菌種培養 28 3.3.1. 無氧培養基 28 3.3.2. 好氧培養基 30 3.3.3. 固態培養基 31 3.3.4. 共培養 32 3.3.5. 純菌計數 33 3.4. 靜電紡絲材料試驗 34 3.4.1. 共軸溶劑配置及電紡方法 34 3.4.2. 單軸溶劑配置及電紡方法 37 3.5. 菌種固定化及保存實驗 39 3.5.1. 濃縮菌液製備 39 3.5.2. 靜電紡絲固定菌種方法 39 3.5.3. 保存方法 40 3.6. DEHP降解實驗 41 3.6.1. DEHP 第一批次降解 41 3.6.2. DEHP 第二批次降解 42 3.7. 降解效果分析 43 3.7.1. 高效液相層析儀 ( HPLC ) 43 3.7.2. 離子層析儀 ( Ion Chromatography, IC ) 45 3.8. 纖維形貌分析 46 3.8.1. 掃描式電子顯微鏡 ( SEM ) 46 3.8.2. 共軛焦螢光顯微鏡 ( Confocal fluorescence microscopy ) 47 第四章 結果與討論 48 4.1 靜電紡絲材料試驗 48 4.1.1 共軸參數可行性評估 48 4.1.2 單軸參數評估及選用 52 4.2 菌種活性評估 54 4.2.1 醋酸纖維素 ( CA ) 54 4.2.2 聚乙烯醇 ( PVA ) 57 4.3 PVA形貌分析 59 4.3.1 共軛焦螢光顯微鏡 ( Confocal fluorescence microscopy ) 59 4.3.2 掃描式電子顯微鏡( SEM ) 60 4.4 第一批次DEHP降解實驗 62 4.4.1 總菌落數 62 4.4.2 硝酸根消耗及污染物去除 63 4.4.3 去除率比較 66 4.5 第二批次DEHP降解實驗 68 4.5.1 總菌落數 68 4.5.2 硝酸根消耗及污染物去除 69 4.5.3 去除率比較 72 第五章 結論與建議 74 5.1 結論 74 5.2 建議 76 參考文獻 77 " | |
| dc.language.iso | zh-TW | |
| dc.subject | 保存 | zh_TW |
| dc.subject | 靜電紡絲 | zh_TW |
| dc.subject | 鄰苯二甲酸二(2-乙基己基)酯 | zh_TW |
| dc.subject | 塑化劑 | zh_TW |
| dc.subject | preserving | en |
| dc.subject | Acidovorax sp. | en |
| dc.subject | Thauera sp. | en |
| dc.subject | Di(2-ethylhexyl) phthalate | en |
| dc.subject | DEHP | en |
| dc.subject | plasticizers | en |
| dc.subject | PVA | en |
| dc.subject | fiber | en |
| dc.title | 利用靜電紡絲固定及保存菌種去除水中塑化劑 (鄰苯二甲酸二(2-乙基己基)酯) | zh_TW |
| dc.title | Biodegradation of Di(2-ethylhexyl) phthalate by immobilized and preserved bacteria with electrospinning | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 王柏翔(Hsin-Tsai Liu),童世煌(Chih-Yang Tseng) | |
| dc.subject.keyword | 塑化劑,鄰苯二甲酸二(2-乙基己基)酯,靜電紡絲,保存, | zh_TW |
| dc.subject.keyword | Acidovorax sp.,Thauera sp.,Di(2-ethylhexyl) phthalate,DEHP,plasticizers,PVA,preserving,fiber, | en |
| dc.relation.page | 83 | |
| dc.identifier.doi | 10.6342/NTU202101370 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-07-13 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 環境工程學研究所 | zh_TW |
| dc.date.embargo-lift | 2023-06-16 | - |
| 顯示於系所單位： | 環境工程學研究所 | |
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