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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79373完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃念祖(Nien-Tsu Huang) | |
| dc.contributor.author | Shang-Jyun Lin | en |
| dc.contributor.author | 林上竣 | zh_TW |
| dc.date.accessioned | 2022-11-23T08:59:08Z | - |
| dc.date.available | 2022-11-01 | |
| dc.date.available | 2022-11-23T08:59:08Z | - |
| dc.date.copyright | 2021-11-02 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-10-26 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79373 | - |
| dc.description.abstract | 為了提供適當的抗生素治療，臨床應用上的標準做法是將從病人檢體分離出來的菌株進行抗藥性檢測（antimicrobial susceptibility test，AST），來選擇合適的抗生素種類和劑量，同時也可以避免抗生素的濫用。然而，目前的AST方法仍有耗時、耗力、低準確性等缺點。為了提高準確性，具有高專一性和免標定等優點的表面增強拉曼散射（surface-enhanced Raman spectroscopy，SERS）開始被應用在細菌檢測和抗藥性檢測上。在本論文中，我們設計了一個微流道裝置，具有可用來建立抗生素濃度梯度的側流道（side channel），及在液體流動時，可將細菌保留在其中的微流井（microwell）。透過使用針筒幫浦同時注入細菌培養液與抗生素溶液，此裝置可以自動建立2、4、8、16、32、64 μg/mL等六個抗生素濃度。在螢光珠模擬細菌的實驗中，得知微流井可保留80%以上的螢光珠避免被沖走。接著，當細菌在建立好的各個抗生素濃度下培養三個小時後，即可整合SERS檢測技術進行AST（SERS-AST）。本論文研究共針對兩株細菌進行AST，分別為氨苄青黴素（ampicillin）非抗藥性與抗藥性的大腸桿菌（Escherichia coli）。所有微流井的SERS訊號皆會被量測，經分析即可測得最小抑菌濃度（minimum inhibitory concentration，MIC），且該結果與標準AST作法的結果一致。此SERS-AST方法不僅只需要少量（20 μL）細菌溶液與單一微流道裝置與即可測得MIC，更是將整個AST過程從一天大幅縮短至5小時，如此一來可以幫助醫生及早修正施用的抗生素種類和劑量，進一步提高病患存活率並減少抗生素的濫用。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-23T08:59:08Z (GMT). No. of bitstreams: 1 U0001-2510202115043100.pdf: 5758413 bytes, checksum: adf53e714ef0acf03d6b0c618c08301f (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 口試委員會審定書 I 致謝 II 摘要 III Abstract IV Chapter 1 Introduction 1 1.1 Research Background 1 1.1.1 Antimicrobial resistance and AST 1 1.1.2 Clinical AST methods 2 1.1.3 Microfluidic AST 3 1.2 Literature Review 4 1.2.1 Bacteria incubation in the microfluidic chip 4 1.2.2 Bacteria quantification methods in microfluidic AST 5 1.2.3 Antibiotic concentration gradient generation in microfluidic AST 12 1.3 Research Motivation 17 Chapter 2 SERS Theory 18 2.1 Introduction of Raman Scattering 18 2.2 Surface-Enhanced Raman Scattering 19 2.3 Bacteria SERS signal source 20 Chapter 3 Materials and Methods 22 3.1 Bacteria Preparation 22 3.2 Device Design and Fabrication 22 3.2.1 Microfluidic device design 23 3.2.2 Microfluidic device fabrication 24 3.2.3 SERS-active substrate fabrication 28 3.3 The Bright-field and Fluorescent Optical Setup 28 3.4 SERS Measurement and Spectral Processing 29 3.5 On-chip MIC Measurement Protocol 29 3.6 Image Analysis 30 3.6.1 Fluorescent molecule quantification 31 3.6.2 Beads number quantification 31 3.6.3 SERS imaging 32 Chapter 4 Results and Discussion 33 4.1 Channel Design 33 4.1.1 260-side-channel-design 33 4.1.2 64-side-channel-design 35 4.2 COMSOL Simulation 38 4.2.1 Flow rate effect 38 4.2.2 Balancing time 41 4.3 Concentration of Gradient Generation 42 4.3.1 Flow rate optimization 42 4.3.2 Channel isolation optimization 44 4.3.3 Microwell isolation optimization 48 4.4 Beads Encapsulation with Microwell Array 51 4.4.1 Trapping efficacy of the side channel 51 4.4.2 Washing efficiency 53 4.5 Bacteria Incubation 55 4.5.1 The bacterial growth evaluation in the PDMS microfluidic chip 55 4.5.2 Evaporation and Rehydration 56 4.6 SERS-AST 59 4.6.1 Alignment 59 4.6.2 SERS-AST without antibiotic gradient 62 4.6.3 SERS-AST with antibiotic gradient 63 Chapter 5 Conclusion 68 Chapter 6 Future Work 69 References 72 Appendix 80 A. Python and ImageJ code 80 A.1 Python 80 A.2 ImageJ 81 | |
| dc.language.iso | en | |
| dc.title | 微流道抗生素濃度梯度產生器整合於表面增強拉曼散射平行進行細菌抗藥性檢測 | zh_TW |
| dc.title | A Microfluidic Antibiotic Concentration Gradient Generator Integrating Surface enhanced Raman Spectroscopy for Multiparallel Antimicrobial Susceptibility Testing | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 王玉麟(Hsin-Tsai Liu),王俊凱(Chih-Yang Tseng),韓吟宜,張祐嘉 | |
| dc.subject.keyword | 微流道,濃度梯度,細菌,抗藥性檢測,表面增強拉曼散射, | zh_TW |
| dc.subject.keyword | microfluidic,concentration gradient,bacteria,antimicrobial susceptibility test,surface-enhanced Raman spectroscopy, | en |
| dc.relation.page | 83 | |
| dc.identifier.doi | 10.6342/NTU202104148 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-10-27 | |
| dc.contributor.author-college | 電機資訊學院 | zh_TW |
| dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
| 顯示於系所單位： | 生醫電子與資訊學研究所 | |
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