請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79368完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃念祖(Nien-Tsu Huang) | |
| dc.contributor.author | Chia-Yu Hsieh | en |
| dc.contributor.author | 謝佳諭 | zh_TW |
| dc.date.accessioned | 2022-11-23T08:59:02Z | - |
| dc.date.available | 2022-11-01 | |
| dc.date.available | 2022-11-23T08:59:02Z | - |
| dc.date.copyright | 2021-11-02 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-10-27 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79368 | - |
| dc.description.abstract | "細菌抗藥性檢測 (Antimicrobial susceptibility test, AST) 是目前評估血流感染 (Bloodstream infection, BSI) 用藥最常使用的方法之一。但目前使用的細菌抗藥性檢測技術需要繁瑣的樣品處理流程及複雜的檢測系統，使整個流程需要二至三天的時間，難以提供即時的藥物決策，錯失有效治療的黃金時期且亦可能會產生抗藥性菌株。為解決上述問題，我們藉由量測微生物的醣類代謝造成菌液的酸化程度來評估細菌的生長情況。本研究選擇表面由高介電常數的二氧化鉿 (HfO2) 組成之雙閘極離子敏感場效電晶體 (Dual-gate ion-sensitive field-effect transistor, DG-ISFET) 量測菌液酸化，因其高介電常數的氧化金屬和雙閘極的結構，能夠在測量的過程中防止漏電流的產生，減少電流飄移 (drifting effect) 所產生的訊號干擾。此外，我們將DG-ISFET結合質子選擇性膜 (Proton selective membrane, PSM) 提高對氫離子之選擇性以避免培養液中其他離子訊號的干擾。此外，我們使用多孔性濾膜來濃縮菌液，增加細菌濃度進而縮短檢測時間。為驗證上述構想，我們首先使用不同 pH 標準液來評估不同 PSM厚度下的DG-ISFET的解析度 (sensitivity) 及穩定性 (stability)。接著進行離子場效電晶體式細菌抗藥性檢測 (ISFET-AST)的測試，主要分為兩個階段進行。第一階段進行無流道細菌抗藥性測試 (Off-chip AST)，目的為驗證PSM在實際細菌樣本中訊號改善的效果，在這個實驗中，直接將250微升 (µL) 的菌液滴在DG-ISFET進行測量。結果顯示此系統可在 30 分鐘內區分不具抗藥性和具抗藥性的大腸桿菌 (Escherichia coli) 菌株，達成執行快速AST的目標，PSM也能夠改善訊號干擾的情況，使用較少的抗生素便能測量出細菌的抗藥性。第二階段則結合0.22微米孔徑的濾膜流道，加入1000 µL的菌液進行菌液濃縮後執行ISFET-AST，結果顯示濃縮的菌液訊號約有兩至三倍的提升，和濃縮的體積成正比。期望未來能將這種可攜式的檢測系統設備用來進行即時和連續性的細菌抗藥性檢測，並將其應用於醫療資源有限或是定點照護的環境中。" | zh_TW |
| dc.description.provenance | Made available in DSpace on 2022-11-23T08:59:02Z (GMT). No. of bitstreams: 1 U0001-2510202118001300.pdf: 4461947 bytes, checksum: 45bd8a77e655d13957f600401435fbdd (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | "口試委員會審定書 II 誌謝…………………………………………………………………………………………………III 摘要…………………………………………………………………………………………………IV Abstract………………………………………………………………………………………………V Chapter 1. Introduction 1 1.1. Research background 1 1.2. Literature review 1 1.2.1. Rapid Antimicrobial Susceptibility Test (RAST) 1 1.2.2. Optical measurement 3 1.2.3. Electrical measurement 4 1.2.4. Mechanical measurement 5 1.2.5. pH/ion measurement 5 1.2.6. Summary for current RAST 11 1.3. Filter-based bacterial separation, purification, and enrichment 12 1.3.1. Bacterial separation 12 1.3.2. Concentration adjustment and enrichment: 13 1.3.3. Signal enhancement 13 1.4. Current challenges and limitations 14 Chapter 2. Experimental Design 16 2.1. The working principle of Ion Sensitive Field Effect Transistor (ISFET) 16 2.2. The working principle of dual-gate ion-sensitive field-effect transistor (DG-ISFET) 19 2.3. The principle of PSM-modified ISFETs 19 Chapter 3. Material and methods 23 3.1. Experimental setup 23 3.2. Device and instrument 24 3.3. Filter-integrated microchamber fabrication 25 3.4. Proton selective membrane (PSM) preparation 26 3.5. Solution preparation 26 3.6. Sample preparation 26 Chapter 4. Results and Discussion 28 4.1. DG-ISFET performance 28 4.1.1. Data acquisition 28 4.1.2. Drain-to-source voltage (Vds) optimization 28 4.1.3. pH sensing performance 29 4.1.4. Chip-to-chip variation evaluation 31 4.1.5. Single-chip repeatability test 32 4.1.6. Thickness optimization 33 4.1.7. PSM to PSM variation evaluation 36 4.2. Minimum inhibitory concentration (MIC) 36 4.3. Off-chip AST 37 4.4. Bacterial acidification rate 40 4.5. pH sensitivity with the channel and filter 42 4.6. Filter capture efficiency 44 4.7. Bacterial sample volume-based current difference 45 4.8. On-chip AST with filter-integrated microchamber 47 4.9. Bacterial growth rate in optical density 48 Chapter 5. Conclusion 50 Chapter 6. Future work 51 6.1. The filter-integrated microchamber 51 6.2. Standardize the PSM fabrication process 51 6.3. Bacterial diversity 52 6.4. Multiplex ion-sensing development 52 References…………………………………………………………………………………………54" | |
| dc.language.iso | en | |
| dc.title | 利用雙閘極敏感場效應電晶體結合氫離子選擇膜以及濾膜流道進行細菌濃縮與抗生素藥敏性檢測 | zh_TW |
| dc.title | A Proton Selective Membrane Deposited Dual-gate Ion-Sensitive Field-Effect Transistor (DG-ISFET) Integrating the Microchamber Embedded Filter Membrane for Bacteria Enrichment and Antimicrobial Susceptibility Test | en |
| dc.date.schoolyear | 109-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林乃君(Hsin-Tsai Liu),于昌平(Chih-Yang Tseng),林致廷 | |
| dc.subject.keyword | 雙閘極敏感場效應電晶體,氫離子選擇膜,細菌濃縮,抗生素藥敏性檢測, | zh_TW |
| dc.subject.keyword | Proton Selective Membrane,DG-ISFET,Bacteria Enrichment,Antimicrobial Susceptibility Test,ISFET, | en |
| dc.relation.page | 63 | |
| dc.identifier.doi | 10.6342/NTU202104166 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2021-10-28 | |
| dc.contributor.author-college | 電機資訊學院 | zh_TW |
| dc.contributor.author-dept | 生醫電子與資訊學研究所 | zh_TW |
| 顯示於系所單位： | 生醫電子與資訊學研究所 | |
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| U0001-2510202118001300.pdf | 4.36 MB | Adobe PDF | 檢視/開啟 |
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