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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 楊台鴻(Tai-Horng Young) | |
dc.contributor.author | Guang-Shih Chen | en |
dc.contributor.author | 陳光世 | zh_TW |
dc.date.accessioned | 2021-07-11T15:50:24Z | - |
dc.date.available | 2021-08-06 | |
dc.date.copyright | 2018-08-06 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-07-27 | |
dc.identifier.citation | 1. Fowkes, F.G.R., et al., Comparison of global estimates of prevalence and risk factors for peripheral artery disease in 2000 and 2010: a systematic review and analysis. The Lancet, 2013. 382(9901): p. 1329-1340.
2. Criqui, M.H. and V. Aboyans, Epidemiology of peripheral artery disease. Circulation research, 2015. 116(9): p. 1509-1526. 3. Nehler, M.R., et al., Epidemiology of peripheral arterial disease and critical limb ischemia in an insured national population. Journal of vascular surgery, 2014. 60(3): p. 686-695. e2. 4. Parikh, P.P., Z.-J. Liu, and O.C. Velazquez, A Molecular and Clinical Review of Stem Cell Therapy in Critical Limb Ischemia. Stem Cells International, 2017. 2017. 5. Becker, F., et al., Chapter I: definitions, epidemiology, clinical presentation and prognosis. European Journal of Vascular and Endovascular Surgery, 2011. 42: p. S4-S12. 6. Kalbaugh, C.A., et al., Peripheral Artery Disease Prevalence and Incidence Estimated From Both Outpatient and Inpatient Settings Among Medicare Fee‐for‐Service Beneficiaries in the Atherosclerosis Risk in Communities (ARIC) Study. Journal of the American Heart Association, 2017. 6(5): p. e003796. 7. Norgren, L., et al., Inter-society consensus for the management of peripheral arterial disease (TASC II). Journal of vascular surgery, 2007. 45(1): p. S5-S67. 8. Kasapis, C. and H.S. Gurm, Current approach to the diagnosis and treatment of femoral-popliteal arterial disease. A systematic review. Current cardiology reviews, 2009. 5(4): p. 296-311. 9. Inampudi, C., et al., Angiogenesis in peripheral arterial disease. Current opinion in pharmacology, 2018. 39: p. 60-67. 10. Hoffman, A.S., Hydrogels for biomedical applications. Advanced drug delivery reviews, 2012. 64: p. 18-23. 11. Hoare, T.R. and D.S. Kohane, Hydrogels in drug delivery: Progress and challenges. Polymer, 2008. 49(8): p. 1993-2007. 12. Campoccia, D., et al., Semisynthetic resorbable materials from hyaluronan esterification. Biomaterials, 1998. 19(23): p. 2101-2127. 13. Prestwich, G.D., et al., Controlled chemical modification of hyaluronic acid: synthesis, applications, and biodegradation of hydrazide derivatives. Journal of Controlled Release, 1998. 53(1-3): p. 93-103. 14. Drumheller, P.D. and J.A. Hubbell, Densely crosslinked polymer networks of poly (ethylene glycol) in trimethylolpropane triacrylate for cell‐adhesion‐resistant surfaces. Journal of Biomedical Materials Research Part A, 1995. 29(2): p. 207-215. 15. Elviri, L., et al., Controlled local drug delivery strategies from chitosan hydrogels for wound healing. Expert opinion on drug delivery, 2017. 14(7): p. 897-908. 16. Bhumkar, D.R. and V.B. Pokharkar, Studies on effect of pH on cross-linking of chitosan with sodium tripolyphosphate: a technical note. Aaps Pharmscitech, 2006. 7(2): p. E138-E143. 17. Mi, F.-L., et al., Synthesis and characterization of biodegradable TPP/genipin co-crosslinked chitosan gel beads. Polymer, 2003. 44(21): p. 6521-6530. 18. Kean, T. and M. Thanou, Biodegradation, biodistribution and toxicity of chitosan. Advanced drug delivery reviews, 2010. 62(1): p. 3-11. 19. Pellá, M.G., et al., Chitosan-based hydrogels: from preparation to biomedical applications. Carbohydrate polymers, 2018. 20. Kumar, M.R., et al., Chitosan chemistry and pharmaceutical perspectives. Chemical reviews, 2004. 104(12): p. 6017-6084. 21. Hoemann, C., et al., Tissue engineering of cartilage using an injectable and adhesive chitosan-based cell-delivery vehicle. Osteoarthritis and cartilage, 2005. 13(4): p. 318-329. 22. Kempe, S., et al., Characterization of thermosensitive chitosan-based hydrogels by rheology and electron paramagnetic resonance spectroscopy. European Journal of Pharmaceutics and Biopharmaceutics, 2008. 68(1): p. 26-33. 23. Kim, I.-Y., et al., Chitosan and its derivatives for tissue engineering applications. Biotechnology advances, 2008. 26(1): p. 1-21. 24. Azuma, K., et al., Chitin, chitosan, and its derivatives for wound healing: old and new materials. Journal of functional biomaterials, 2015. 6(1): p. 104-142. 25. Baldrick, P., The safety of chitosan as a pharmaceutical excipient. Regulatory toxicology and pharmacology, 2010. 56(3): p. 290-299. 26. Jayakumar, R., et al., Biomaterials based on chitin and chitosan in wound dressing applications. Biotechnology advances, 2011. 29(3): p. 322-337. 27. Djagny, K.B., Z. Wang, and S. Xu, Gelatin: a valuable protein for food and pharmaceutical industries. Critical reviews in food science and nutrition, 2001. 41(6): p. 481-492. 28. Gómez-Guillén, M., et al., Functional and bioactive properties of collagen and gelatin from alternative sources: A review. Food hydrocolloids, 2011. 25(8): p. 1813-1827. 29. Klotz, B.J., et al., Gelatin-methacryloyl hydrogels: towards biofabrication-based tissue repair. Trends in biotechnology, 2016. 34(5): p. 394-407. 30. Lai, J.-Y. and Y.-T. Li, Functional assessment of cross-linked porous gelatin hydrogels for bioengineered cell sheet carriers. Biomacromolecules, 2010. 11(5): p. 1387-1397. 31. Matthyssen, S., et al., Corneal regeneration: A review of stromal replacements. Acta biomaterialia, 2018. 32. Mimura, T., et al., Tissue engineering of corneal stroma with rabbit fibroblast precursors and gelatin hydrogels. Molecular vision, 2008. 14: p. 1819. 33. Vandooren, J., P.E. Van den Steen, and G. Opdenakker, Biochemistry and molecular biology of gelatinase B or matrix metalloproteinase-9 (MMP-9): the next decade. Critical reviews in biochemistry and molecular biology, 2013. 48(3): p. 222-272. 34. Heino, J., et al., Evolution of collagen-based adhesion systems. The international journal of biochemistry & cell biology, 2009. 41(2): p. 341-348. 35. Nichol, J.W., et al., Cell-laden microengineered gelatin methacrylate hydrogels. Biomaterials, 2010. 31(21): p. 5536-5544. 36. Totre, J., D. Ickowicz, and A.J. Domb, Properties and hemostatic application of gelatin. Biodegradable Polymers in Clinical Use and Clinical Development, 2011: p. 91-109. 37. G Tahrir, F., F. Ganji, and T. M Ahooyi, Injectable thermosensitive chitosan/glycerophosphate-based hydrogels for tissue engineering and drug delivery applications: a review. Recent patents on drug delivery & formulation, 2015. 9(2): p. 107-120. 38. Kim, S., et al., A chitosan/β-glycerophosphate thermo-sensitive gel for the delivery of ellagic acid for the treatment of brain cancer. Biomaterials, 2010. 31(14): p. 4157-4166. 39. Jarry, C., et al., Effects of steam sterilization on thermogelling chitosan‐based gels. Journal of Biomedical Materials Research Part A, 2001. 58(1): p. 127-135. 40. Barsotti, M.C., et al., Effect of platelet lysate on human cells involved in different phases of wound healing. PLoS One, 2013. 8(12): p. e84753. 41. Dessels, C., M. Potgieter, and M.S. Pepper, Making the switch: alternatives to fetal bovine serum for adipose-derived stromal cell expansion. Frontiers in Cell and Developmental Biology, 2016. 4: p. 115. 42. Sovkova, V., et al., Platelet lysate as a serum replacement for skin cell culture on biomimetic PCL nanofibers. Platelets, 2017: p. 1-11. 43. Atashi, F., et al., Autologous platelet-rich plasma: a biological supplement to enhance adipose-derived mesenchymal stem cell expansion. Tissue Engineering Part C: Methods, 2014. 21(3): p. 253-262. 44. Li, H., et al., Autologous platelet-rich plasma promotes neurogenic differentiation of human adipose-derived stem cells in vitro. International Journal of Neuroscience, 2013. 123(3): p. 184-190. 45. Chen, L.W., et al., The corneal epitheliotrophic abilities of lyophilized powder form human platelet lysates. PloS one, 2018. 13(3): p. e0194345. 46. Van Pham, P., et al., Activated platelet-rich plasma improves adipose-derived stem cell transplantation efficiency in injured articular cartilage. Stem cell research & therapy, 2013. 4(4): p. 91. 47. Bieback, K., Platelet lysate as replacement for fetal bovine serum in mesenchymal stromal cell cultures. Transfusion Medicine and Hemotherapy, 2013. 40(5): p. 326-335. 48. Hofbauer, P., et al., Human platelet lysate is a feasible candidate to replace fetal calf serum as medium supplement for blood vascular and lymphatic endothelial cells. Cytotherapy, 2014. 16(9): p. 1238-1244. 49. Naskou, M.C., et al., Platelet lysate as a novel serum-free media supplement for the culture of equine bone marrow-derived mesenchymal stem cells. Stem cell research & therapy, 2018. 9(1): p. 75. 50. Saury, C., et al., Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches. Stem cell research & therapy, 2018. 9(1): p. 128. 51. Pignatelli, C., et al., Electrospun silk fibroin fibers for storage and controlled release of human platelet lysate. Acta biomaterialia, 2018. 73: p. 365-376. 52. Robinson, S.T., et al., A novel platelet lysate hydrogel for endothelial cell and mesenchymal stem cell-directed neovascularization. Acta biomaterialia, 2016. 36: p. 86-98. 53. Huang, C.-J., et al., Comparison of corneal epitheliotrophic capacities among human platelet lysates and other blood derivatives. PloS one, 2017. 12(2): p. e0171008. 54. Golebiewska, E.M. and A.W. Poole, Platelet secretion: From haemostasis to wound healing and beyond. Blood reviews, 2015. 29(3): p. 153-162. 55. Mussano, F., et al., Cytokine, chemokine, and growth factor profile of platelet-rich plasma. Platelets, 2016. 27(5): p. 467-471. 56. Nurden, A.T., et al., Platelets and wound healing. Frontiers in bioscience: a journal and virtual library, 2008. 13: p. 3532-3548. 57. Ghobril, C. and M. Grinstaff, The chemistry and engineering of polymeric hydrogel adhesives for wound closure: a tutorial. Chemical Society Reviews, 2015. 44(7): p. 1820-1835. 58. Lai, T.C., J. Yu, and W.B. Tsai, Gelatin methacrylate/carboxybetaine methacrylate hydrogels with tunable crosslinking for controlled drug release. Journal of Materials Chemistry B, 2016. 4(13): p. 2304-2313. 59. Murphy, K.C., et al., Engineering fibrin hydrogels to promote the wound healing potential of mesenchymal stem cell spheroids. Acta biomaterialia, 2017. 64: p. 176-186. 60. Sys, G.M., et al., The in ovo CAM-assay as a xenograft model for sarcoma. Journal of visualized experiments: JoVE, 2013(77). 61. Schlatter, P., et al., Quantitative study of intussusceptive capillary growth in the chorioallantoic membrane (CAM) of the chicken embryo. Microvascular research, 1997. 54(1): p. 65-73. 62. Ribatti, D., The chick embryo chorioallantoic membrane (CAM). A multifaceted experimental model. Mechanisms of development, 2016. 141: p. 70-77. 63. Ribatti, D., et al., Erythropoietin is involved in angiogenesis in human primary melanoma. International journal of experimental pathology, 2010. 91(6): p. 495-499. 64. Pacelli, S., et al., Nanodiamond-based injectable hydrogel for sustained growth factor release: preparation, characterization and in vitro analysis. Acta biomaterialia, 2017. 58: p. 479-491. 65. Nguyen, M., Y. Shing, and J. Folkman, Quantitation of angiogenesis and antiangiogenesis in the chick embryo chorioallantoic membrane. Microvascular research, 1994. 47(1): p. 31-40. 66. Vázquez, F., et al., METH-1, a human ortholog of ADAMTS-1, and METH-2 are members of a new family of proteins with angio-inhibitory activity. Journal of Biological Chemistry, 1999. 274(33): p. 23349-23357. 67. Nowak-Sliwinska, P., T. Segura, and M.L. Iruela-Arispe, The chicken chorioallantoic membrane model in biology, medicine and bioengineering. Angiogenesis, 2014. 17(4): p. 779-804. 68. Berger, J., et al., Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications. European Journal of Pharmaceutics and Biopharmaceutics, 2004. 57(1): p. 19-34. 69. Hennink, W.E. and C.F. van Nostrum, Novel crosslinking methods to design hydrogels. Advanced drug delivery reviews, 2012. 64: p. 223-236. 70. Bhattarai, N., J. Gunn, and M. Zhang, Chitosan-based hydrogels for controlled, localized drug delivery. Advanced drug delivery reviews, 2010. 62(1): p. 83-99. 71. Kulkarni, A.R., et al., A Novel Method for the Synthesis of the PEG‐Crosslinked Chitosan with a pH‐Independent Swelling Behavior. Macromolecular bioscience, 2005. 5(10): p. 925-928. 72. Aminabhavi, T. and S. Dharupaneedi, Production of chitosan-based hydrogels for biomedical applications, in Chitosan Based Biomaterials Volume 1. 2017, Elsevier. p. 295-319. 73. Jin, J., M. Song, and D. Hourston, Novel chitosan-based films cross-linked by genipin with improved physical properties. Biomacromolecules, 2004. 5(1): p. 162-168. 74. Chiono, V., et al., Genipin-crosslinked chitosan/gelatin blends for biomedical applications. Journal of Materials Science: Materials in Medicine, 2008. 19(2): p. 889-898. 75. Koo, H.-J., et al., Antiinflammatory effects of genipin, an active principle of gardenia. European journal of pharmacology, 2004. 495(2-3): p. 201-208. 76. Hoemann, C., et al., Cytocompatible gel formation of chitosan‐glycerol phosphate solutions supplemented with hydroxyl ethyl cellulose is due to the presence of glyoxal. Journal of biomedical materials research Part A, 2007. 83(2): p. 521-529. 77. Vaz, C.M., et al., In vitro degradation behaviour of biodegradable soy plastics: effects of crosslinking with glyoxal and thermal treatment. Polymer Degradation and Stability, 2003. 81(1): p. 65-74. 78. Wang, L. and J.P. Stegemann, Glyoxal crosslinking of cell-seeded chitosan/collagen hydrogels for bone regeneration. Acta biomaterialia, 2011. 7(6): p. 2410-2417. 79. Chenite, A., et al., Novel injectable neutral solutions of chitosan form biodegradable gels in situ. Biomaterials, 2000. 21(21): p. 2155-2161. 80. Ganji, F., M. Abdekhodaie, and A.R. SA, Gelation time and degradation rate of chitosan-based injectable hydrogel. Journal of sol-gel science and technology, 2007. 42(1): p. 47-53. 81. Xin, X., et al., Hyaluronic-acid-based semi-interpenetrating materials. Journal of Biomaterials Science, Polymer Edition, 2004. 15(9): p. 1223-1236. 82. Dessi, M., et al., Novel biomimetic thermosensitive β‐tricalcium phosphate/chitosan‐based hydrogels for bone tissue engineering. Journal of Biomedical Materials Research Part A, 2013. 101(10): p. 2984-2993. 83. Chiang, H.-S., et al., Lycopene inhibits PDGF-BB-induced signaling and migration in human dermal fibroblasts through interaction with PDGF-BB. Life sciences, 2007. 81(21-22): p. 1509-1517. 84. Siedlecki, J., et al., Combined VEGF and PDGF inhibition for neovascular AMD: anti-angiogenic properties of axitinib on human endothelial cells and pericytes in vitro. Graefe's Archive for Clinical and Experimental Ophthalmology, 2017. 255(5): p. 963-972. 85. Erber, R., et al., Combined inhibition of VEGF and PDGF signaling enforces tumor vessel regression by interfering with pericyte-mediated endothelial cell survival mechanisms. The FASEB journal, 2004. 18(2): p. 338-340. 86. Ponce, M.L., Tube formation: an in vitro matrigel angiogenesis assay, in Angiogenesis Protocols. 2009, Springer. p. 183-188. 87. Arnaoutova, I., et al., The endothelial cell tube formation assay on basement membrane turns 20: state of the science and the art. Angiogenesis, 2009. 12(3): p. 267-274. 88. Shen, K., et al., Involvement of c‐Jun N‐terminal kinase and extracellular signal‐regulated kinase 1/2 in EGF‐induced angiogenesis. Cell biology international, 2010. 34(12): p. 1213-1218. 89. Wojtowicz, A.M., et al., The importance of both fibroblasts and keratinocytes in a bilayered living cellular construct used in wound healing. Wound Repair and Regeneration, 2014. 22(2): p. 246-255. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79176 | - |
dc.description.abstract | 近年來,周邊性動脈阻塞性疾病的患者逐漸增加,但目前臨床上對於此疾病患者的治療方式大多以支架、氣球將阻塞的動脈撐開或進行血管繞道手術,病情較嚴重的患者更可能面臨到截肢的選擇。為了促進周邊性組織的血液循環,本研究將生醫材料製作成水膠的形式,使用乙二醛將甲殼素與明膠相互交聯進而包覆血小板裂解液,期望能於周邊性組織緩慢釋放生長因子並促進血管新生。為了要鑑定此系統釋放包覆物的釋放機制,本研究包覆不同分子量大小的螢光異硫氰酸鹽右旋醣酐及血小板裂解液進行分析,另一方面,於細胞實驗中我們發現血小板裂解液不僅能夠促進人類臍動脈內皮細胞和纖維母細胞細胞株(HS68)的移動,亦能使人類臍動脈內皮細胞形成管柱。此外,在受精卵及仿真皮膚系統中,我們發現血小板裂解液結合生醫材料於促進血管新生方面具有相當大的潛能,未來值得繼續研究。 | zh_TW |
dc.description.abstract | More and more people suffered from peripheral artery disease (PAD) due to insufficient blood supply to the legs. Currently, the standard therapy for improving blood flow to the affected extremity is either surgical or endovascular revascularization. However, their therapeutic effects are sometimes limited and still many people with PAD may require amputation. The purpose of our research is to combine biomaterials with human platelet lysate (HPL) to increase the blood flow in the ischemia area. In our study, we developed a chitosan-gelatin hydrogel crosslinked by glyoxal for sustained release HPL. To investigate the release profile of this hydrogel system, we used hydrogel to incorporate the FITC-dextran with different molecular weight and the release pattern were determined. On the other hand, the angiogenic effect of HPL was studied in the tube formation assay and transwell migration assay of human umbilical vein endothelial cells (HUVEC). Moreover, supplementing culture medium with HPL induced the migration of HS68 cells in an in vitro wound healing assay suggesting the potential of HPL to facilitate wound closure. Chick chorioallantoic membrane (CAM) assay revealed that HPL can stimulate angiogenesis in vivo. Given the results of in vitro and in vivo experiments, we conclude that the hydrogel-based system of HPL has great potential to increase the blood flow for the treatment of ischemic wounds. | en |
dc.description.provenance | Made available in DSpace on 2021-07-11T15:50:24Z (GMT). No. of bitstreams: 1 ntu-107-R05548030-1.pdf: 3334290 bytes, checksum: 86ca04c8ad9348ad03b671e1f3e1277a (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 中文摘要 ii
Abstract iii Contents iv List of figures vi Chapter 1: Introduction 1 1.1 Peripheral artery disease (PAD) 1 1.2 Hydrogel 3 1.2.1 Hydrogel introduction 3 1.2.2 Chitosan 5 1.2.3 Gelatin 6 1.2.3 β-glycerophosphate (β-GP )& glyoxal 7 1.3 Human platelet lysate (HPL) 8 1.4 Motivation and Aims 10 1.5 Research Framework 11 Chapter 2: Materials and methods 12 Materials 12 Chemicals and Reagents 12 Cell Culture 13 Experimental Equipment 13 Methods 14 2.1 Preparation of chitosan/gelatin based (CS-GE) hydrogels 14 2.2 Rheological studies 15 2.4 Studies of the cargoes release pattern in hydrogels 16 2.5 Chemical cross-linker biocompatibility studies 17 2.6 The effect of HPL on cell proliferation 18 2.7 HS68 cells in vitro wound healing migration assay 19 2.8 HUVEC Transwell migration assay 20 2.9 HUVEC tube formation assay: 21 2.10 3D skin equivalent model 21 2.11 Chick Chorioallantoic Membrane Assay (CAM Assay) 22 2.12 Statistical analysis 23 Chapter 3: Results 24 3.1 Rheological studies 24 3.2 In vitro degradation test 24 3.3 Human platelet lysate release 25 3.4 FITC-Dextran release 25 3.5 Biocompatibility test of glyoxal 27 3.6 Human platelet lysate promote the cell activity of HUVEC/HS68 cells 27 3.7 HS68 cells wound healing migration assay 28 3.8 Human platelet lysate facilitate the migration of HUVEC 29 3.9 Human platelet lysate enhance tube formation in endothelial cells 29 3.10 3D human skin equivalent model 30 3.11 CAM assay 30 Chapter 4: Discussion 32 4.1 Glyoxal as chemical cross-linker in a hydrogel system 32 4.2 Rheological studies 33 4.3 HS68 cells migration 34 4.4 HUVEC migration 34 4.5 HUVEC tube formation 34 4.6 3D human skin equivalent model 35 4.7 CAM assay 36 Chapter 5: Conclusion 37 References 38 Appendix 45 | |
dc.language.iso | en | |
dc.title | 化學交聯水膠包覆人類血小板裂解液於促進血管新生之探討 | zh_TW |
dc.title | Developing a glyoxal-crosslinked hydrogel for sustained release of human platelet lysate to promote angiogenesis | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 鄭乃禎(Nai-Chen Cheng) | |
dc.contributor.oralexamcommittee | 陳偉勵(Wei-Li Chen) | |
dc.subject.keyword | 幾丁聚醣,明膠,血小版裂解液,血管新生, | zh_TW |
dc.subject.keyword | chitosan,gelatin,human platelet lysate,angiogenesis, | en |
dc.relation.page | 57 | |
dc.identifier.doi | 10.6342/NTU201801993 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-07-30 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
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