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標題: | 以交聯型生醫材料開發緩釋血小板濃縮液之醫材促進肌腱韌帶修補組織介面之癒合 Development and evaluation of cross-linked PRP-loaded granules for sustained-release in the healing of orthopedic tissue interfaces by repairing tendons and ligaments |
作者: | Sheng-Hao Tseng 曾盛豪 |
指導教授: | 楊台鴻(Tai-Horng Young) |
關鍵字: | 血小板濃厚液,生長因子,生醫材料,控制釋放,肌腱-骨介面癒合, PRP,growth factors,biomaterials,controlled release,tendon-bone healing, |
出版年 : | 2018 |
學位: | 碩士 |
摘要: | 臨床上前十字韌帶重建手術雖可以由病人自體之髕骨肌腱或腿後肌肌腱,取代受傷之韌帶重建韌帶組織,但由於缺乏血管移植的肌腱組織在低氧和低營養的環境,延長了移植組織和肌腱-骨頭間(tendon-bone)癒合的修復時間,而有移植失敗之風險。因此,若能在手術過程中搭配有效的促進療法,必能提高重建手術的效果。
血小板濃厚液(platelet-rich plasma, PRP)富含生長因子,已被大量使用於退化性關節炎之病患,應可作為加速前十字韌帶重建時之韌帶癒合、tendon-bone修復的方法。但流體狀的PRP注入於患部時,會流散開來無法停滯於患部,因此,以生醫材料作為PRP包覆之載體,使PRP包覆於顆粒內,除減緩其流動性,能有助於緩慢釋出PRP,使PRP能在患部有較長時間釋出生長因子,達到較佳的治療效果。 故本研究探討生醫材料結合PRP後,前十字韌帶纖維母細胞於低血清與低氧氣環境中的活性( cell viability )、增生( proliferation )及遷移( migration )能力,並建立兔子的tendon-bonem癒合模型,進行PRP微粒在體外和體內實驗的評估。 Clinical anterior cruciate ligament reconstruction surgery often use autologous patellar tendon or hamstring (semitendinosus) graft to replace the ruptured ligament. However, since the transplanted tissue does not have an intact blood supply and is in low oxygen and nutrient environment, the graft and tendon-bone healing time will be extended with a high risk of failed surgery. Therefore, the purpose of the project is to provide a medical innovation combined with biomaterial and platelet-rich plasma (PRP) to improve the result of reconstructive surgery. PRP, rich in growth factors, has been extensively used in patients with degenerative arthritis so it is available as a tool to enhance ligament and tendon-bone healing at ligament reconstruction surgery. To control the fluid-like property of PRP, which is not able to stagnate in the affected part, this study will encapsulate PRP by an appropriate biomaterial to sluggish its fluidity and to control growth factors release from PRP beads. It is anticipated that PRP can be maintained in the affected part for a longer time and the better therapeutic effect is achieved. Therefore, this study explored the cell viability、proliferation ability and migration ability of the anterior cruciate ligament cells by combining biomaterials and PRP in low serum and low oxygen environment and to establish rabbit ligament healing model. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79142 |
DOI: | 10.6342/NTU201802338 |
全文授權: | 有償授權 |
電子全文公開日期: | 2023-08-06 |
顯示於系所單位: | 醫學工程學研究所 |
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