請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79118
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳漪紋(Yi-Wen Chen) | |
dc.contributor.author | Ya-Ting Jhang | en |
dc.contributor.author | 張雅婷 | zh_TW |
dc.date.accessioned | 2021-07-11T15:45:25Z | - |
dc.date.available | 2021-10-09 | |
dc.date.copyright | 2018-10-09 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-08-08 | |
dc.identifier.citation | Akiyama, F., Aoki, A., Miura-Uchiyama, M., Sasaki, K. M., Ichinose, S., Umeda, M., . . . Izumi, Y. (2011). In vitro studies of the ablation mechanism of periodontopathic bacteria and decontamination effect on periodontally diseased root surfaces by erbium:yttrium-aluminum-garnet laser. Lasers Med Sci, 26(2), 193-204. doi:10.1007/s10103-010-0763-3
Aoki, A., Ando, Y., Watanabe, H., & Ishikawa, I. (1994). In vitro studies on laser scaling of subgingival calculus with an erbium:YAG laser. J Periodontol, 65(12), 1097-1106. doi:10.1902/jop.1994.65.12.1097 Aoki, A., Miura, M., Akiyama, F., Nakagawa, N., Tanaka, J., Oda, S., . . . Ishikawa, I. (2000). In vitro evaluation of Er:YAG laser scaling of subgingival calculus in comparison with ultrasonic scaling. J Periodontal Res, 35(5), 266-277. Aoki, A., Mizutani, K., Schwarz, F., Sculean, A., Yukna, R. A., Takasaki, A. A., . . . Izumi, Y. (2015). Periodontal and peri-implant wound healing following laser therapy. Periodontol 2000, 68(1), 217-269. doi:10.1111/prd.12080 Apatzidou, D. A., & Kinane, D. F. (2010). Nonsurgical mechanical treatment strategies for periodontal disease. Dent Clin North Am, 54(1), 1-12. doi:10.1016/j.cden.2009.08.006 Belanger, M., Rodrigues, P. H., Dunn, W. A., Jr., & Progulske-Fox, A. (2006). Autophagy: a highway for Porphyromonas gingivalis in endothelial cells. Autophagy, 2(3), 165-170. Bodet, C., Chandad, F., & Grenier, D. (2007). Pathogenic potential of Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia, the red bacterial complex associated with periodontitis. Pathol Biol (Paris), 55(3-4), 154-162. doi:10.1016/j.patbio.2006.07.045 Cao, J., Wang, T., Pu, Y., Tang, Z., & Meng, H. (2018). Influence on proliferation and adhesion of human gingival fibroblasts from different titanium surface decontamination treatments: An in vitro study. Arch Oral Biol, 87, 204-210. doi:10.1016/j.archoralbio.2017.12.013 den Braber, E. T., de Ruijter, J. E., Smits, H. T., Ginsel, L. A., von Recum, A. F., & Jansen, J. A. (1995). Effect of parallel surface microgrooves and surface energy on cell growth. J Biomed Mater Res, 29(4), 511-518. doi:10.1002/jbm.820290411 Dorn, B. R., Dunn, W. A., Jr., & Progulske-Fox, A. (2002). Bacterial interactions with the autophagic pathway. Cell Microbiol, 4(1), 1-10. Eick, S., Meier, I., Spoerle, F., Bender, P., Aoki, A., Izumi, Y., . . . Sculean, A. (2017). In Vitro-Activity of Er:YAG Laser in Comparison with other Treatment Modalities on Biofilm Ablation from Implant and Tooth Surfaces. PLoS One, 12(1), e0171086. doi:10.1371/journal.pone.0171086 Eick, S., Ramseier, C. A., Rothenberger, K., Bragger, U., Buser, D., & Salvi, G. E. (2016). Microbiota at teeth and implants in partially edentulous patients. A 10-year retrospective study. Clin Oral Implants Res, 27(2), 218-225. doi:10.1111/clr.12588 Froum, S. J., & Rosen, P. S. (2012). A proposed classification for peri-implantitis. Int J Periodontics Restorative Dent, 32(5), 533-540. Furst, M. M., Salvi, G. E., Lang, N. P., & Persson, G. R. (2007). Bacterial colonization immediately after installation on oral titanium implants. Clin Oral Implants Res, 18(4), 501-508. doi:10.1111/j.1600-0501.2007.01381.x Gao, A., Wang, X., Yu, H., Li, N., Hou, Y., & Yu, W. (2016). Effect of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on the expression of EphA2 in osteoblasts and osteoclasts. In Vitro Cell Dev Biol Anim, 52(2), 228-234. doi:10.1007/s11626-015-9965-0 Giannelli, M., Chellini, F., Margheri, M., Tonelli, P., & Tani, A. (2008). Effect of chlorhexidine digluconate on different cell types: a molecular and ultrastructural investigation. Toxicol In Vitro, 22(2), 308-317. doi:10.1016/j.tiv.2007.09.012 Goldman, L., Hornby, P., Meyer, R., & Goldman, B. (1964). Impact of the laser on dental caries. Nature, 203, 417. Green, J., Weiss, A., & Stern, A. (2011). Lasers and radiofrequency devices in dentistry. Dent Clin North Am, 55(3), 585-597, ix-x. doi:10.1016/j.cden.2011.02.017 Guyodo, H., Meuric, V., Le Pottier, L., Martin, B., Faili, A., Pers, J. O., & Bonnaure-Mallet, M. (2012). Colocalization of Porphyromonas gingivalis with CD4+ T cells in periodontal disease. FEMS Immunol Med Microbiol, 64(2), 175-183. doi:10.1111/j.1574-695X.2011.00877.x Hajishengallis, G., Darveau, R. P., & Curtis, M. A. (2012). The keystone-pathogen hypothesis. Nat Rev Microbiol, 10(10), 717-725. doi:10.1038/nrmicro2873 Holt, S. C., & Ebersole, J. L. (2005). Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia: the 'red complex', a prototype polybacterial pathogenic consortium in periodontitis. Periodontol 2000, 38, 72-122. doi:10.1111/j.1600-0757.2005.00113.x Kong, S., Aoki, A., Iwasaki, K., Mizutani, K., Katagiri, S., Suda, T., . . . Izumi, Y. (2018). Biological effects of Er:YAG laser irradiation on the proliferation of primary human gingival fibroblasts. J Biophotonics, 11(3). doi:10.1002/jbio.201700157 Kononen, M., Hormia, M., Kivilahti, J., Hautaniemi, J., & Thesleff, I. (1992). Effect of surface processing on the attachment, orientation, and proliferation of human gingival fibroblasts on titanium. J Biomed Mater Res, 26(10), 1325-1341. doi:10.1002/jbm.820261006 Kreisler, M., Al Haj, H., & d'Hoedt, B. (2002). Temperature changes at the implant-bone interface during simulated surface decontamination with an Er:YAG laser. Int J Prosthodont, 15(6), 582-587. Kreisler, M., Kohnen, W., Marinello, C., Gotz, H., Duschner, H., Jansen, B., & d'Hoedt, B. (2002). Bactericidal effect of the Er:YAG laser on dental implant surfaces: an in vitro study. J Periodontol, 73(11), 1292-1298. doi:10.1902/jop.2002.73.11.1292 Kunzler, T. P., Drobek, T., Schuler, M., & Spencer, N. D. (2007). Systematic study of osteoblast and fibroblast response to roughness by means of surface-morphology gradients. Biomaterials, 28(13), 2175-2182. doi:10.1016/j.biomaterials.2007.01.019 Lamont, R. J., Chan, A., Belton, C. M., Izutsu, K. T., Vasel, D., & Weinberg, A. (1995). Porphyromonas gingivalis invasion of gingival epithelial cells. Infect Immun, 63(10), 3878-3885. Lee, B. S., Shih, K. S., Lai, C. H., Takeuchi, Y., & Chen, Y. W. (2018). Surface property alterations and osteoblast attachment to contaminated titanium surfaces after different surface treatments: An in vitro study. Clin Implant Dent Relat Res. doi:10.1111/cid.12624 Lin, T., Kawamura, R., Aoki, A., Ichinose, S., Mizutani, K., Taniguchi, Y., . . . Izumi, Y. (2016). Energy output reduction and surface alteration of quartz tips following Er:YAG laser contact irradiation on soft and hard tissues in vitro. Dent Mater J, 35(1), 51-62. doi:10.4012/dmj.2015-020 Madeira, M. F., Queiroz-Junior, C. M., Cisalpino, D., Werneck, S. M., Kikuchi, H., Fujise, O., . . . Souza, D. G. (2013). MyD88 is essential for alveolar bone loss induced by Aggregatibacter actinomycetemcomitans lipopolysaccharide in mice. Mol Oral Microbiol, 28(6), 415-424. doi:10.1111/omi.12034 Mason, M. L. (1992). Using the laser for implant maintenance. Dent Today, 11(4), 74-75. Matsuyama, T., Aoki, A., Oda, S., Yoneyama, T., & Ishikawa, I. (2003). Effects of the Er:YAG laser irradiation on titanium implant materials and contaminated implant abutment surfaces. J Clin Laser Med Surg, 21(1), 7-17. doi:10.1089/10445470360516680 Meire, M. A., Coenye, T., Nelis, H. J., & De Moor, R. J. (2012). In vitro inactivation of endodontic pathogens with Nd:YAG and Er:YAG lasers. Lasers Med Sci, 27(4), 695-701. doi:10.1007/s10103-011-0940-z Mizutani, K., Aoki, A., Takasaki, A. A., Kinoshita, A., Hayashi, C., Oda, S., & Ishikawa, I. (2006). Periodontal tissue healing following flap surgery using an Er:YAG laser in dogs. Lasers Surg Med, 38(4), 314-324. doi:10.1002/lsm.20299 Nominzul Batsukh, S. W. F., Wei Fang Lee. (2017). Effects of Porphyromonas gingivalis on Titanium Surface by Different Clinical Treatment. Journal of Medical and Biological Engineering, 37(1), 35-44. Ogawa, T., & Yagi, T. (2010). Bioactive mechanism of Porphyromonas gingivalis lipid A. Periodontol 2000, 54(1), 71-77. doi:10.1111/j.1600-0757.2009.00343.x Ogita, M., Tsuchida, S., Aoki, A., Satoh, M., Kado, S., Sawabe, M., . . . Izumi, Y. (2015). Increased cell proliferation and differential protein expression induced by low-level Er:YAG laser irradiation in human gingival fibroblasts: proteomic analysis. Lasers Med Sci, 30(7), 1855-1866. doi:10.1007/s10103-014-1691-4 Perez-Chaparro, P. J., Duarte, P. M., Shibli, J. A., Montenegro, S., Lacerda Heluy, S., Figueiredo, L. C., . . . Feres, M. (2016). The Current Weight of Evidence of the Microbiologic Profile Associated With Peri-Implantitis: A Systematic Review. J Periodontol, 87(11), 1295-1304. doi:10.1902/jop.2016.160184 Potempa, J., Sroka, A., Imamura, T., & Travis, J. (2003). Gingipains, the major cysteine proteinases and virulence factors of Porphyromonas gingivalis: structure, function and assembly of multidomain protein complexes. Curr Protein Pept Sci, 4(6), 397-407. Quirynen, M., Vogels, R., Peeters, W., van Steenberghe, D., Naert, I., & Haffajee, A. (2006). Dynamics of initial subgingival colonization of 'pristine' peri-implant pockets. Clin Oral Implants Res, 17(1), 25-37. doi:10.1111/j.1600-0501.2005.01194.x Ramazanoglu, M., Oshida, Y. (2011). Implant Dentistry - A Rapidly Evolving Practice: InTech. Renvert, S., Roos-Jansaker, A. M., & Claffey, N. (2008). Non-surgical treatment of peri-implant mucositis and peri-implantitis: a literature review. J Clin Periodontol, 35(8 Suppl), 305-315. doi:10.1111/j.1600-051X.2008.01276.x Sarmiento, H. L., Norton, M. R., & Fiorellini, J. P. (2016). A Classification System for Peri-implant Diseases and Conditions. Int J Periodontics Restorative Dent, 36(5), 699-705. doi:10.11607/prd.2918 Schwarz, F., Rothamel, D., & Becker, J. (2003). Influence of an Er:YAG laser on the surface structure of titanium implants. Schweiz Monatsschr Zahnmed, 113(6), 660-671. Schwarz, F., Sahm, N., Iglhaut, G., & Becker, J. (2011). Impact of the method of surface debridement and decontamination on the clinical outcome following combined surgical therapy of peri-implantitis: a randomized controlled clinical study. J Clin Periodontol, 38(3), 276-284. doi:10.1111/j.1600-051X.2010.01690.x Schwarz, F., Sculean, A., Berakdar, M., Georg, T., Reich, E., & Becker, J. (2003a). Clinical evaluation of an Er:YAG laser combined with scaling and root planing for non-surgical periodontal treatment. A controlled, prospective clinical study. J Clin Periodontol, 30(1), 26-34. Schwarz, F., Sculean, A., Berakdar, M., Georg, T., Reich, E., & Becker, J. (2003b). Periodontal treatment with an Er:YAG laser or scaling and root planing. A 2-year follow-up split-mouth study. J Periodontol, 74(5), 590-596. doi:10.1902/jop.2003.74.5.590 Shibli, J. A., Melo, L., Ferrari, D. S., Figueiredo, L. C., Faveri, M., & Feres, M. (2008). Composition of supra- and subgingival biofilm of subjects with healthy and diseased implants. Clin Oral Implants Res, 19(10), 975-982. doi:10.1111/j.1600-0501.2008.01566.x Taniguchi, Y., Aoki, A., Sakai, K., Mizutani, K., Meinzer, W., & Izumi, Y. (2016). A Novel Surgical Procedure for Er:YAG Laser-Assisted Periodontal Regenerative Therapy: Case Series. Int J Periodontics Restorative Dent, 36(4), 507-515. doi:10.11607/prd.2515 Tomasi, C., Schander, K., Dahlen, G., & Wennstrom, J. L. (2006). Short-term clinical and microbiologic effects of pocket debridement with an Er:YAG laser during periodontal maintenance. J Periodontol, 77(1), 111-118. doi:10.1902/jop.2006.77.1.111 Yamaguchi, H., Kobayashi, K., Osada, R., Sakuraba, E., Nomura, T., Arai, T., & Nakamura, J. (1997). Effects of irradiation of an erbium:YAG laser on root surfaces. J Periodontol, 68(12), 1151-1155. doi:10.1902/jop.1997.68.12.1151 Yan, M., Liu, M., Wang, M., Yin, F., & Xia, H. (2015). The effects of Er:YAG on the treatment of peri-implantitis: a meta-analysis of randomized controlled trials. Lasers Med Sci, 30(7), 1843-1853. doi:10.1007/s10103-014-1692-3 Yilmaz, O., Verbeke, P., Lamont, R. J., & Ojcius, D. M. (2006). Intercellular spreading of Porphyromonas gingivalis infection in primary gingival epithelial cells. Infect Immun, 74(1), 703-710. doi:10.1128/IAI.74.1.703-710.2006 Yilmaz, S., Kut, B., Gursoy, H., Eren-Kuru, B., Noyan, U., & Kadir, T. (2012). Er:YAG laser versus systemic metronidazole as an adjunct to nonsurgical periodontal therapy: a clinical and microbiological study. Photomed Laser Surg, 30(6), 325-330. doi:10.1089/pho.2010.2762 Zhao, Y., Yin, Y., Tao, L., Nie, P., Tang, Y., & Zhu, M. (2014). Er:YAG laser versus scaling and root planing as alternative or adjuvant for chronic periodontitis treatment: a systematic review. J Clin Periodontol, 41(11), 1069-1079. doi:10.1111/jcpe.12304 Zitzmann, N. U., & Berglundh, T. (2008). Definition and prevalence of peri-implant diseases. J Clin Periodontol, 35(8 Suppl), 286-291. doi:10.1111/j.1600-051X.2008.01274.x | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79118 | - |
dc.description.abstract | 背景:人工植牙使用於重建咬合已經有60 年的歷史,研究指出植體周圍炎的盛行率很高,而目前尚沒有一個有效的方式去完全解決植體周圍炎的問題,因此本研究想要藉由鉺雅鉻雷射的介入去觀察模擬植體周圍炎的鈦板其滅菌情形及其後續的細胞貼附結果。
實驗材料與方法:本實驗使用四級純鈦的鈦板,共有六個組別,第一組是對照組只有鈦板,第二到第六組接種P. gingivalis模擬植體周圍炎。第三組將其使用0.12%的氯己定做沖洗;第四組使用鈦金屬刮匙做清創;第五組使用鉺雅鉻雷射做滅菌;第六組先使用鈦金屬刮匙再合併鉺雅鉻雷射做滅菌。接著使用電子顯微鏡觀察鈦板表面的情形,並藉由螢光染色觀察P. gingivalis活/死菌的分佈情形,以及測量表面粗糙度及接觸角的分析,並觀察不同處理後細菌的內毒素是否有改變,後續在螢光顯微鏡下進行牙齦纖維母細胞貼附的觀察。 實驗結果:實驗的研究結果顯示,單純使用0.12%的氯己定無論是滅菌效果或細胞貼附情形都不佳。鈦金屬刮匙的滅菌效果雖不錯但對於細菌內毒素似乎作用有限,且鈦板的表面被改變。鈦金屬刮匙與雷射對於移除細菌同樣有效,雖然電子顯微鏡的定性分析顯示雷射組別殘存細菌量較少,但兩組在螢光分析上並未有統計上的顯著差異。第五組和第六組經雷射處理的結果,內毒素減少的效果為最佳。細胞貼附的情形以第六組為最佳,但和第四組及第五組並不具有統計上的顯著差異。 結論:就目前的研究結果來說,鉺雅鉻雷射可以有效於移除鈦板上地細菌和內毒素對於後續人類牙齦纖維母細胞貼附情形都有極佳的結果,後續還會使用骨母細胞做進一步的測試與分析。 | zh_TW |
dc.description.abstract | Studies have reported a high prevalence of peri-implantitis with radiographic marginal bone loss. Evidence to date indicates that no available treatments result in total resolution of established peri-implantitis. The aim of this study was to investigate the bactericidal effect and fibroblast attachment on contaminated titanium surface treated with erbium-doped yttrium aluminium garnet (Er:YAG) laser.
Materials and Methods: Grade IV titanium discs were incubated with a suspension of Porphyromonas gingivalis (P. gingivalis) (ATCC® 33277TM) in brain heart infusion (BHI) broth. Six groups (n=6) with a total of 36 titanium discs were prepared. Group 1 was the negative control. Group 2 to 6, titanium discs were incubated with P. gingivalis biofilm. Group 3, 0.12 % Chlorhexidine was used for irrigation. Group 4, titanium curette was used for debridement. Group 5, Er:YAG laser irradiation was performed at pulse energy 80 mJ and frequency 25 pulse per second. Group 6, Curette debridement was followed by Er:YAG laser irradiation. After various treatments, surface roughness and hydrophilicity were measured. The residual bacterial was examined by scanning electron microscope and confocal laser scanning microscope, and the residual lipopolysaccharide was examined by Lymulus amoebocyte lysate (LAL) assay. Then, the adhered human gingival fibroblast (HGF) cells were quantified by fluorescent microscope. Results: Chlorhexidine irrigation or curette debridement alone couldn’t remove bacteria efficiently. Titanium curette could remove bacteria efficiently, but had limited effect on LPS. Er:YAG laser alone could remove bacteria as effective as titanium curette, and Er:YAG laser was most effective in removing LPS. Er:YAG laser combined with curette debridement has highest amount of adhered HGF cells. Though there was no significant difference between titanium curette, Er:YAG laser, and combined group. Conclusion: Er:YAG laser would be a promising therapy to treat peri-implantitis by removing bacterial remnants without altering titanium surface. Further studies are warranted to evaluate the potential of osteoblast adhesion after Er:YAG laser treatment. | en |
dc.description.provenance | Made available in DSpace on 2021-07-11T15:45:25Z (GMT). No. of bitstreams: 1 ntu-107-R04422019-1.pdf: 4349224 bytes, checksum: 30133c40f775bc3caaea9467ec5ef8ef (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 誌謝 i
摘要 v Abstract vi 目錄 viii 圖目錄 x 前言 1 第一章 文獻回顧 2 1.1 植體周圍疾病(Peri-implant disease) 2 1.1.1 植體周圍致病菌 2 1.1.2 Porphyromonas gingivalis之特性 3 1.2雷射介紹 6 1.2.1 牙科雷射 6 1.2.2鉺雅鉻雷射 7 1.2.3鉺雅鉻雷射於牙周病的治療 8 1.2.4鉺雅鉻雷射於植體周圍炎的治療 10 第二章 實驗動機與目的 12 2.1 研究動機 12 2.2 研究目的 12 第三章 實驗材料與方法 13 3.1 實驗材料 13 3.2 實驗儀器 20 3.3 實驗流程圖 21 3.4 鈦金屬板之處理 22 3.5 細菌實驗 22 3.6 模擬臨床移除生物薄膜之處理 26 3.7 細胞實驗 28 3.8 表面內毒素殘留分析 32 3.9 表面接觸角分析 34 3.10 表面粗糙度分析 36 第四章 實驗結果 38 4.1 SEM觀察不同清創處理後之鈦板表面 38 4.2 共軛焦顯微鏡觀察清創處理後鈦板表面活菌死菌分佈情形 41 4.3模擬臨床移除生物薄膜處理後之細胞貼附定量試驗 45 4.4 表面內毒素殘留分析 50 4.5 表面接觸角分析 52 4.6 表面粗糙度分析 54 4.7 SEM觀察不同清創處理後之鈦板表面 55 第五章 討論 57 5.1 鉺雅鉻雷射的輸出功率 57 5.2 0.12 % CHX沖洗與滅菌效果的關係 58 5.3 影響HGF細胞貼附的可能原因 58 5.4 鉺雅鉻雷射的殺菌效果與細胞貼附情形 60 5.4 In vitro研究中與臨床操作的差異 61 5.5未來的展望 61 第六章 結論 62 第七章 參考文獻 63 | |
dc.language.iso | zh-TW | |
dc.title | 探討鉺雅鉻雷射對粗糙鈦板表面牙周病致病菌的殺菌效果和細胞貼附能力 | zh_TW |
dc.title | In vitro evaluation of the bactericidal effect and fibroblast attachment on contaminated rough titanium surfaces treated with Er : YAG laser | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 郭彥彬(Mark Yen-Ping Kuo),林泰誠(Tai-Chen Lin) | |
dc.subject.keyword | 鉺雅鉻雷射,牙齦??菌,人類牙齦纖維母細胞,粗糙鈦金屬表面,植體周圍炎, | zh_TW |
dc.subject.keyword | Er:YAG laser,Porphyromonas gingivalis,human gingival fibroblast,rough titanium surface,peri-implantitis, | en |
dc.relation.page | 67 | |
dc.identifier.doi | 10.6342/NTU201802650 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-08-08 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
顯示於系所單位: | 臨床牙醫學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-107-R04422019-1.pdf 目前未授權公開取用 | 4.25 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。