請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79041
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳敏慧 | zh_TW |
dc.contributor.advisor | en | |
dc.contributor.author | 張丰毓 | zh_TW |
dc.contributor.author | Karen Chang | en |
dc.date.accessioned | 2021-07-11T15:38:59Z | - |
dc.date.available | 2024-02-28 | - |
dc.date.copyright | 2018-10-11 | - |
dc.date.issued | 2018 | - |
dc.date.submitted | 2002-01-01 | - |
dc.identifier.citation | [1] Sweeney, A.E., “Nanomedicine concepts in the general medical curriculum: initiating a discussion.” Int J Nanomedicine, 2015. 10: 7319-31.
[2] Boulaiz, H., Alvarez, P.J., Ramirez, A., Marchal, J.A., Prados, J., Rodríguez-Serrano, F., Perán, M., Melguizo, C., and Aranega, A., “Nanomedicine: application areas and development prospects.” Int J Mol Sci, 2011. 12(5): 3303-3321. [3] Zhang, L., Gu, F.X., Chan, J.M., Wang, A.Z., Langer, R.S., and Farokhzad, O.C., “Nanoparticles in medicine: Therapeutic applications and developments.” Clin Pharmacol Ther, 2008. 83: 761–769. [4] Chang, E.H., Harford, J.B., Eaton, M.A., Boisseau, P.M., Dube, A., Hayeshi, R., Swai, H., and Lee, D.S., “Nanomedicine: Past, present and future - A global perspective.” Biochem Biophys Res Commun, 2015. 468(3): 511-7. [5] Langer, R., and Tirrell, D.A., “Designing materials for biology and medicine.” Nature, 2004. 428(6982): 487-92. [6] Boverhof, D.R., Bramante, C.M., Butala, J.H., Clancy, S.F., Lafranconi, M., West, J., and Gordon, S.C., “Comparative assessment of nanomaterial definitions and safety evaluation considerations.” Regul Toxicol Pharmacol, 2015. 73(1): 137-50. [7] Laurent, S., Bridot, J.L., Elst, L.V., and Muller, R.N., “Magnetic iron oxide nanoparticles for biomedical applications.” Future Med Chem, 2010. 2(3): 427-49. [8] Mahmoudi, M., Hosseinkhani, H., Hosseinkhani, M., Boutry, S., Simchi, A., Journeay, W.S., Subramani, K., and Laurent, S., “Magnetic resonance imaging tracking of stem cells in vivo using iron oxide nanoparticles as a tool for the advancement of clinical regenerative medicine”. Chem Rev, 2011. 111(2): 253-80. [9] Tiwari, A., Verma, N.C., Singh, A., Nandi, C.K., and Randhawa, J.K., “Carbon coated core-shell multifunctional fluorescent SPIONs.” Nanoscale, 2018. 10(22): 10389-10394. [10] Dykman, L., and Khlebtsov, N., “Gold nanoparticles in biomedical applications: recent advances and perspectives.” Chem Soc Rev, 2012. 41(6): 2256-82. [11] Daraee, H., Eatemadi, A., Abbasi, E., Fekri Aval, S., Kouhi, M., and Akbarzadeh, A., “Application of gold nanoparticles in biomedical and drug delivery.” Artif Cells Nanomed Biotechnol, 2016. 44(1): 410-22. [12] Zhou, J., Yang, Y., and Zhang, C.Y., “Toward Biocompatible Semiconductor Quantum Dots: From Biosynthesis and Bioconjugation to Biomedical Application.” Chem Rev, 2015. 115(21): 11669-717. [13] Bajwa, N., Mehra, N.K., Jain, K., and Jain, N.K., “Pharmaceutical and biomedical applications of quantum dots.” Artif Cells Nanomed Biotechnol, 2016. 44(3): 758-768. [14] Gong, H., Peng, R., and Liu, Z., “Carbon nanotubes for biomedical imaging: the recent advances.” Adv Drug Deliv Rev, 2013. 65(15): 1951-63. [15] Heister, E., Brunner, E.W., Dieckmann, G.R., Jurewicz, I., and Dalton, A.B., “Are carbon nanotubes a natural solution? Applications in biology and medicine.” ACS Appl Mater Interfaces, 2013. 5(6): 1870-91. [16] McGuinness, L.P., Yan, Y., Stacey, A., Simpson, D.A., Hall, L.T., Maclaurin, D., Prawer, S., Mulvaney, P., Wrachtrup, J., Caruso, F., Scholten, R.E., and Hollenberg, L.C. “Quantum measurement and orientation tracking of fluorescent nanodiamonds inside living cells.” Nat Nanotechnol, 2011. 6(6): 358-363. [17] Whitlow, J., Pacelli, S., and Paul, A., “Multifunctional nanodiamonds in regenerative medicine: Recent advances and future directions.” J Control Release, 2017. 261: 62-86. [18] Yong, K.T., Law, W.C., Hu, R., Ye, L., Liu, L., Swihart, M.T., and Prasad, P.N., “Nanotoxicity assessment of quantum dots: from cellular to primate studies.” Chem Soc Rev, 2013. 42(3): 1236-50. [19] Chatterjee, K., Sarkar, S., Jagajjanani Rao, K., and Paria, S., “Core/shell nanoparticles in biomedical applications.” Adv Colloid Interface Sci, 2014. 209: 8-39. [20] Zhao, Y., Zhao, X., Cheng, Y., Guo, X., and Yuan, W., “Iron Oxide Nanoparticles-Based Vaccine Delivery for Cancer Treatment.” Mol Pharm, 2018. 15(5):1791-99. [21] Liu, X., Marangon, I., Melinte, G., Wilhelm, C., Ménard-Moyon, C., Pichon, B.P., Ersen, O., Aubertin, K., Baaziz, W., Pham-Huu, C., Bégin-Colin, S., Bianco, A., Gazeau, F., and Bégin, D., “Design of covalently functionalized carbon nanotubes filled with metal oxide nanoparticles for imaging, therapy, and magnetic manipulation.” ACS Nano, 2014. 8(11): 11290-304. [22] Colombo, M., Carregal-Romero, S., Casula, M.F., Gutiérrez, L., Morales, M.P., Böhm, I.B., Heverhagen, J.T., Prosperi, D., and Parak, W.J., “Biological applications of magnetic nanoparticles.” Chem Soc Rev, 2012. 41(11): 4306-34. [23] Ling, D., Lee, N., and Hyeon, T., “Chemical synthesis and assembly of uniformly sized iron oxide nanoparticles for medical applications.” Acc Chem Res, 2015. 48(5): 1276-85. [24] Quinto, C.A., Mohindra, P., Tong, S., and Bao, G., “Multifunctional superparamagnetic iron oxide nanoparticles for combined chemotherapy and hyperthermia cancer treatment.” Nanoscale, 2015. 7(29): 12728-36. [25] Espinosa, A., Di Corato, R., Kolosnjaj-Tabi, J., Flaud, P., Pellegrino, T., and Wilhelm, C., “Duality of Iron Oxide Nanoparticles in Cancer Therapy: Amplification of Heating Efficiency by Magnetic Hyperthermia and Photothermal Bimodal Treatment.” ACS Nano, 2016. 10(2): 2436-46. [26] Kogure, K., Moriguchi, R., Sasaki, K., Ueno, M., Futaki, S., and Harashima, H., “Development of a non-viral multifunctional envelope-type nano device by a novel lipid film hydration method.” J Control Release, 2004. 98(2): 317-23. [27] Kogure, K., Akita, H., and Harashima, H., “Multifunctional envelope-type nano device for non-viral gene delivery: concept and application of Programmed Packaging.” J Control Release, 2007. 122(3): 246-251. [28] Kogure, K., Akita, H., Yamada, Y., and Harashima, H., “Multifunctional envelope-type nano device (MEND) as a non-viral gene delivery system.” Adv Drug Deliv Rev, 2008. 60(4-5): 559-571. [29] Sato, Y., Nakamura, T., Yamada, Y., and Harashima, H., “Development of a multifunctional envelope-type nano device and its application to nanomedicine.” J Control Release, 2016. 244(Pt B): 194-204. [30] Richard, C., Balavoine, F., Schultz, P., Ebbesen, T.W., and Mioskowski, C., “Supramolecular self-assembly of lipid derivatives on carbon nanotubes.” Science, 2003. 300(5620): 775-8. [31] Chang, C.F., Chen, C.Y., Chang, F.H., Tai, S.P., Chen, C.Y., Yu, C.H., Tseng, Y.B., Tsai, T.H., Liu, I.S., Su, W.F., and Sun, C.K., “Cell tracking and detection of molecular expression in live cells using lipid-enclosed CdSe quantum dots as contrast agents for epi-third harmonic generation microscopy.” Opt Express, 2008. 16(13): 9534-48. [32] Vankayala, R., Chiang, C.S., Chao, J.I., Yuan, C.J., Lin, S.Y., and Hwang, K.C., “A general strategy to achieve ultra-high gene transfection efficiency using lipid-nanoparticle composites.” Biomaterials, 2014. 35(28): 8261-72. [33] Wang, S., Yang, W., Du, H., Guo, F., Wang, H., Chang, J., Gong, X., and Zhang, B., “Multifunctional reduction-responsive SPIO&DOX-loaded PEGylated polymeric lipid vesicles for magneticresonance imaging-guided drug delivery.” Nanotechnology, 2016. 27(16): 165101. [34] Behr, J.P., “Synthetic gene transfer vectors II: back to the future.” Acc Chem Res, 2012. 45(7): 980-4. [35] Ramamoorth, M., and Narvekar, A., “Non-viral vectors in gene therapy- an overview.” J Clin Diagn Res, 2015. 9(1): GE01-6. [36] Deshmukh, H.M., and Huang, L. “Liposome and polylysine mediated gene transfer.” New J Chem, 1997. 21(1): 113-124. [37] Miller, A.D., “Cationic liposomes for gene therapy.” Angew Chem Int Ed, 1998. 37(13-14): 1768-1785. [38] Johnsson, M., and Edwards, K., “Phase behavior and aggregate structure in mixtures of dioleoylphosphatidylethanolamine and poly(ethylene glycol)-lipids.” Biophys J, 2001. 80(1): 313-23. [39] Zhu, L., Kate, P., and Torchilin, V.P., “Matrix metalloprotease 2-responsive multifunctional liposomal nanocarrier for enhanced tumor targeting.” ACS Nano, 2012. 6(4): 3491-8. [40] Zhou, H., Fan, Z., Deng, J., Lemons, P.K., Arhontoulis, D.C., Bowne, W.B., and Cheng, H., “Hyaluronidase Embedded in Nanocarrier PEG Shell for Enhanced Tumor Penetration and Highly Efficient Antitumor Efficacy.” Nano Lett, 2016. 16(5): 3268-77. [41] Wang, J., Li, S., Han, Y., Guan, J., Chung, S., Wang, C., and Li, D., “Poly(Ethylene Glycol)-Polylactide Micelles for Cancer Therapy.” Front Pharmacol, 2018. 9: 202. [42] Arteaga, C.L., Sliwkowski, M.X., Osborne, C.K., Perez, E.A., Puglisi, F., and Gianni, L., “Treatment of HER2-positive breast cancer: current status and future perspectives.” Nat Rev Clin Oncol, 2011. 9(1): 16-32. [43] Choi, W.I., Lee, J.H., Kim, J.Y., Heo, S.U., Jeong, Y.Y., Kim, Y.H., and Tae, G., “Targeted antitumor efficacy and imaging via multifunctional nano-carrier conjugated with anti-HER2 trastuzumab.” Nanomedicine, 2015. 11(2): 359-68. [44] Palanca-Wessels, M.C., Booth, G.C., Convertine, A.J., Lundy, B.B., Berguig, G.Y., Press, M.F., Stayton, P.S., and Press, O.W., “Antibody targeting facilitates effective intratumoral siRNA nanoparticle delivery to HER2-overexpressing cancer cells.” Oncotarget, 2016. 7(8): 9561-75. [45] Dvir, T., Timko, B.P., Kohane, D.S., and Langer, R., “Nanotechnological strategies for engineering complex tissues.” Nat Nanotechnol, 2011. 6(1): 13-22. [46] Sampath, U.G.T.M., Ching, Y.C., Chuah, C.H., Sabariah, J.J., and Lin, P.C., “Fabrication of Porous Materials from Natural/Synthetic Biopolymers and Their Composites.” Materials (Basel), 2016. 9(12) pii: E991. [47] O'Brien, F.J., “Biomaterials & scaffolds for tissue engineering.” Mater Today, 2011. 14(3): 57-120 [48] Chen, P.H., Liao, H.C., Hsu, S.H., Chen, R.S., Wu, M.C., Yang, Y.F., Wu, C.C., Chen, M.H., and Su, W.F., “A novel polyurethane/cellulose fibrous scaffold for cardiac tissue engineering.” Pharmaceut Med, 2011. 25(5): 293-306. [49] Madduri, S., Papaloïzos, M., and Gander, B., “Trophically and topographically functionalized silk fibroin nerve conduits for guided peripheral nerve regeneration.” Biomaterials, 2010. 31(8): 2323-34. [50] Wittmer, C.R., Claudepierre, T., Reber, M., Wiedemann, P., Garlick, J.A., Kaplan, D., and Egles, C., “Multifunctionalized electrospun silk fibers promote axon regeneration in central nervous system.” Adv Funct Mater, 2011. 21(22): 4202. [51] Kador, K.E., Montero, R.B., Venugopalan, P., Hertz, J., Zindell, A.N., Valenzuela, D.A., Uddin, M.S., Lavik, E.B., Muller, K.J., Andreopoulos, F.M., and Goldberg, J.L., “Tissue engineering the retinal ganglion cell nerve fiber layer.” Biomaterials, 2013. 34(17): 4242-50. [52] Verma, I.M., and Weitzman, M.D., “Gene therapy: twenty-first century medicine.” Annu Rev Biochem, 2005. 74: 711-38. [53] Kaufmann, K.B., Büning, H., Galy, A., Schambach, A., and Grez, M., “Gene therapy on the move.” EMBO Mol Med, 2013. 5(11): 1642-61. [54] Wang, D., and Gao, G., “State-of-the-art human gene therapy: part I. Gene delivery technologies.” Discov Med, 2014. 18(97): 67-77. [55] Kamimura, K., Suda, T., Zhang, G., and Liu, D., “Advances in Gene Delivery Systems.” Pharmaceut Med, 2011. 25(5): 293-306. [56] Nayerossadat, N., Maedeh, T., and Ali, PA., “Viral and nonviral delivery systems for gene delivery.” Adv Biomed Res, 2012. 1:27. [57] Nimesh, S., Halappanavar, S., Kaushik, N.K., and Kumar, P., “Advances in gene delivery systems.” Biomed Res Int, 2015. 2015: 610342. [58] Huang, S., and Kamihira, M., “Development of hybrid viral vectors for gene therapy.” Biotechnol Adv, 2013. 31(2): 208-23. [59] Check, E., “Harmful potential of viral vectors fuels doubts over gene therapy.” Nature, 2003. 423(6940): 573-4. [60] Giacca, M., Zacchigna, S., “Virus-mediated gene delivery for human gene therapy.” J Control Release, 2012. 161(2): 377-88. [61] Yin, H., Kanasty, R.L., Eltoukhy, A.A., Vegas, A.J., Dorkin, J.R., and Anderson D.G., “Non-viral vectors for gene-based therapy.” Nat Rev Genet, 2014. 15(8): 541-55. [62] Henriques, A.M., Madeira, C., Fevereiro, M., Prazeres, D.M., Aires-Barros, M.R., and Monteiro, G.A., “Effect of cationic liposomes/DNA charge ratio on gene expression and antibody response of a candidate DNA vaccine against Maedi Visna virus.” Int J Pharm. 2009. 377(1-2): 92-8. [63] Jin, L., Zeng, X., Liu, M., Deng, Y., He, N., “Current progress in gene delivery technology based on chemical methods and nano-carriers.” Theranostics, 2014. 4(3): 240-55. [64] Singh, B.N., Prateeksha, Gupta, V.K., Chen, J., and Atanasov, A.G., “Organic Nanoparticle-Based Combinatory Approaches for Gene Therapy.” Trends Biotechnol, 2017. 35(12): 1121-1124. [65] Kaushik, G., Leijten, J., and Khademhosseini, A., “Concise Review: Organ Engineering: Design, Technology, and Integration.” Stem Cells, 2017. 35(1):51-60. [66] Martin, I., Wendt, D., and Heberer, M., “The role of bioreactors in tissue engineering.” Trends Biotechnol, 2004. 22(2): 80-6. [67] Yannas, I.V., Lee, E., Orgill, D.P., Skrabut, E.M., and Murphy, G.F., “Synthesis and characterization of a model extracellular matrix that induces partial regeneration of adult mammalian skin.” Proc Natl Acad Sci U S A, 1989. 86(3): 933-7. [68] Seifert, A.W., Kiama, S.G., Seifert, M.G., Goheen, J.R., Palmer, T.M., and Maden, M., “Skin shedding and tissue regeneration in African spiny mice (Acomys).” Nature, 2012. 489(7417): 561-5. [69] Fishman, J.M., Wiles, K., Lowdell, M.W., De Coppi, P., Elliott, M.J., Atala, A., and Birchall, M.A., “Airway tissue engineering: an update.” Expert Opin Biol Ther, 2014. 14(10): 1477-91. [70] Lam Van Ba, O., Aharony, S., Loutochin, O., and Corcos, J., “Bladder tissue engineering: a literature review.” Adv Drug Deliv Rev, 2015. 82-83: 31-7. [71] Warnke, P.H., Springer, I.N., Wiltfang, J., Acil, Y., Eufinger, H., Wehmöller, M., Russo, P.A., Bolte, H., Sherry, E., Behrens, E., and Terheyden, H., “Growth and transplantation of a custom vascularised bone graft in a man.” Lancet, 2004. 364(9436): 766-70. [72] Trautvetter, W., Kaps, C., Schmelzeisen, R., Sauerbier, S., and Sittinger, M., “Tissue-engineered polymer-based periosteal bone grafts for maxillary sinus augmentation: five-year clinical results.” J Oral Maxillofac Surg, 2011. 69(11): 2753-62. [73] Brunello, G., Sivolella, S., Meneghello, R., Ferroni, L., Gardin, C., Piattelli, A., Zavan, B., and Bressan, E., “Powder-based 3D printing for bone tissue engineering.” Biotechnol Adv, 2016. 34(5): 740-753. [74] Orlando, G., Baptista, P., Birchall, M., De Coppi, P., Farney, A., Guimaraes-Souza, N.K., Opara, E., Rogers, J., Seliktar, D., Shapira-Schweitzer, K., Stratta, R.J., Atala, A., Wood, K.J., and Soker, S., “Regenerative medicine as applied to solid organ transplantation: current status and future challenges.” Transpl Int, 2011. 24(3): 223-32. [75] Welman, T., Michel, S., Segaren, N., and Shanmugarajah, K., “Bioengineering for Organ Transplantation: Progress and Challenges.” Bioengineered, 2015. 6(5): 257-61. [76] Schmidt, C.E., and Leach, J.B., “Neural tissue engineering: strategies for repair and regeneration.” Annu Rev Biomed Eng, 2003. 5: 293-347. [77] Jiang, X., Lim, S.H., Mao, H.Q., and Chew, S.Y., “Current applications and future perspectives of artificial nerve conduits.” Exp Neurol, 2010. 223(1): 86-101. [78] Luo, L., He, Y., Wang, X., Key, B., Lee, B.H., Li, H., and Ye, Q., “Potential Roles of Dental Pulp Stem Cells in Neural Regeneration and Repair.” Stem Cells Int, 2018. 2018: 1731289. [79] Silva, G.A., “Neuroscience nanotechnology: progress, opportunities and challenges.” Nat Rev Neurosci, 2006. 7(1): 65-74. [80] Gronthos, S., Mankani, M., Brahim, J, Robey, P.G., and Shi, S., “Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo.” Proc Natl Acad Sci USA, 2000. 97(25): 13625-30. [81] Miura, M., Gronthos, S., Zhao, M., Lu, B., Fisher, L.W., Robey, P.G., and Shi, S., “SHED: stem cells from human exfoliated deciduous teeth.” Proc Natl Acad Sci USA, 2003. 100(10): 5807-12. [82] Seo, B.M., Miura, M., Gronthos, S., Bartold, P.M., Batouli, S., Brahim, J., Young, M., Robey, P.G., Wang, C.Y., and Shi, S., “Investigation of multipotent postnatal stem cells from human periodontal ligament.” Lancet, 2004. 364(9429): 149-55. [83] Sonoyama, W., Liu, Y., Fang, D., Yamaza, T., Seo, B.M., Zhang, C., Liu, H., Gronthos, S., Wang, C.Y., Wang, S., and Shi, S., “Mesenchymal stem cell-mediated functional tooth regeneration in swine.” PLoS One, 2006. 1: e79. [84] Morsczeck, C., Götz, W., Schierholz, J., Zeilhofer, F., Kühn, U., Möhl, C., Sippel, C., and Hoffmann, K.H., “Isolation of precursor cells (PCs) from human dental follicle of wisdom teeth.” Matrix Biol, 2005. 24(2): 155-65. [85] Estrela, C., Alencar, A.H., Kitten, G.T., Vencio, E.F., and Gava, E., “Mesenchymal stem cells in the dental tissues: perspectives for tissue regeneration.” Braz Dent J, 2011. 22(2): 91-8. [86] Liu, J., Yu, F., Sun, Y., Jiang, B., Zhang, W., Yang, J., Xu, G.T., Liang, A., and Liu, S., “Concise reviews: Characteristics and potential applications of human dental tissue-derived mesenchymal stem cells.” Stem Cells, 2015. 33(3): 627-38. [87] Gronthos, S., Brahim, J., Li, W., Fisher, L.W., Cherman, N., Boyde, A., DenBesten, P., Robey, P.G., and Shi, S., “Stem cell properties of human dental pulp stem cells.” J Dent Res, 2002. 81(8): 531-5. [88] Bartold, P.M., McCulloch, C.A., Narayanan, A.S., and Pitaru, S., “Tissue engineering: a new paradigm for periodontal regeneration based on molecular and cell biology.” Periodontol 2000, 2000. 24: 253-69. [89] Sonoyama, W., Liu, Y., Yamaza, T., Tuan, R.S., Wang, S., Shi, S., and Huang, G.T., “Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: a pilot study.” J Endod, 2008. 34(2): 166-71. [90] Lakshmipathy, U., and Verfaillie, C., “Stem cell plasticity.” Blood Rev, 2005. 19(1): 29-38. [91] Liu, S.P., Fu, R.H., Yu, H.H., Li, K.W., Tsai, C.H., Shyu, W.C., and Lin, S.Z., “MicroRNAs regulation modulated self-renewal and lineage differentiation of stem cells.” Cell Transplant, 2009. 18(9): 1039-45. [92] Gong, Q., Wang, R., Jiang, H., Lin, Z., and Ling, J., “Alteration of microRNA expression of human dental pulp cells during odontogenic differentiation.” J Endod, 2012. 38(10): 1348-54. [93] Kumar, M.S., Lu, J., Mercer, K.L., Golub, T.R., and Jacks, T., “Impaired microRNA processing enhances cellular transformation and tumorigenesis.” Nat Genet, 2007. 39(5): 673-7. [94] Yin, K., Hacia, J.G., Zhong, Z., and Paine, M.L., “Genome-wide analysis of miRNA and mRNA transcriptomes during amelogenesis.” BMC Genomics, 2014. 15: 998. [95] Wan, M., Gao, B., Sun, F., Tang, Y., Ye, L., Fan, Y., Klein, O.D., Zhou, X., and Zheng, L., “microRNA miR-34a regulates cytodifferentiation and targets multi-signaling pathways in human dental papilla cells.” PLoS One, 2012. 7(11): e50090. [96] Sun, F., Wan, M., Xu, X., Gao, B., Zhou, Y., Sun, J., Cheng, L., Klein, O.D., Zhou, X., and Zheng, L., “Crosstalk between miR-34a and Notch Signaling Promotes Differentiation in Apical Papilla Stem Cells (SCAPs).” J Dent Res, 2014. 93(6): 589-95. [97] Kim, E.J., Lee, M.J., Li, L., Yoon, K.S., Kim, K.S., and Jung, H.S., “Failure of Tooth Formation Mediated by miR-135a Overexpression via BMP Signaling.” J Dent Res, 2014. 93(6): 571-5. [98] Liu, H., Lin, H., Zhang, L., Sun, Q., Yuan, G., Zhang, L., Chen, S., and Chen, Z., “miR-145 and miR-143 regulate odontoblast differentiation through targeting Klf4 and Osx genes in a feedback loop.” J Biol Chem, 2013. 288(13): 9261-71. [99] Wang, K., Li, L., Wu, J., Qiu, Q., Zhou, F., and Wu, H., “The different expression profiles of microRNAs in elderly and young human dental pulp and the role of miR-433 in human dental pulp cells.” Mech Ageing Dev, 2015. 146-148: 1-11. [100] Li, A., Song, T., Wang, F., Liu, D., Fan, Z., Zhang, C., He, J., and Wang, S., “MicroRNAome and expression profile of developing tooth germ in miniature pigs.” PLoS One, 2012. 7(12): e52256. [101] Yu, H., Gao, G., Jiang, L., Guo, L., Lin, M., Jiao, X., Jia, W., and Huang, J., “Decreased expression of miR-218 is associated with poor prognosis in patients with colorectal cancer.” Int J Clin Exp Pathol, 2013. 6(12): 2904-11. [102] Shi, Z.M., Wang, L., Shen, H., Jiang, C.F., Ge, X., Li, D.M., Wen, Y.Y., Sun, H.R., Pan, M.H., Li, W., Shu, Y.Q., Liu, L.Z., Peiper, S.C., He, J., and Jiang, B.H., “Downregulation of miR-218 contributes to epithelial-mesenchymal transition and tumor metastasis in lung cancer by targeting Slug/ZEB2 signaling.” Oncogene, 2017. 36(18): 2577-2588. [103] Zhang, X., Dong, J., He, Y., Zhao, M., Liu, Z., Wang, N., Jiang, M., Zhang, Z., Liu, G., Liu, H., Nie, Y., Fan, D., and Tie, J., “miR-218 inhibited tumor angiogenesis by targeting ROBO1 in gastric cancer.” Gene, 2017. 615: 42-49. [104] Lu, Y.F., Zhang, L., Waye, M.M., Fu, W.M., and Zhang, J.F., “MiR-218 mediates tumorigenesis and metastasis: Perspectives and implications.” Exp Cell Res, 2015. 334(1): 173-82. [105] Li, N., Wang, L., Tan, G., Guo, Z., Liu, L., Yang, M., and He, J., “MicroRNA-218 inhibits proliferation and invasion in ovarian cancer by targeting Runx2.” Oncotarget, 2017. 8(53): 91530-91541. [106] van Wijnen, A.J., van de Peppel, J., van Leeuwen, J.P., Lian, J.B., Stein, G.S., Westendorf, J.J., Oursler, M.J., Im, H.J., Taipaleenmäki, H., Hesse, E., Riester, S., and Kakar, S., “MicroRNA functions in osteogenesis and dysfunctions in osteoporosis.” Curr Osteoporos Rep, 2013. 11(2): 72-82. [107] Lai, P., Song, Q., Yang, C., Li, Z., Liu, S., Liu, B., Li, M., Deng, H., Cai, D., Jin, D., Liu, A., and Bai, X., “Loss of Rictor with aging in osteoblasts promotes age-related bone loss.” Cell Death Dis, 2016. 7(10): e2408. [108] Gay, I., Cavender, A., Peto, D., Sun, Z., Speer, A., Cao, H., and Amendt, B.A., “Differentiation of human dental stem cells reveals a role for microRNA-218.” J Periodontal Res, 2014. 49(1): 110-20. [109] Rasband, M.N., “Glial Contributions to Neural Function and Disease.” Mol Cell Proteomics, 2016. 15(2): 355-61. [110] Karaöz, E., Demircan, P.C., Sağlam, O., Aksoy, A., Kaymaz, F., and Duruksu, G., “Human dental pulp stem cells demonstrate better neural and epithelial stem cell properties than bone marrow-derived mesenchymal stem cells.” Histochem Cell Biol, 2011. 136(4): 455-73. [111] Mayo, V., Sawatari, Y., Huang, C.Y., and Garcia-Godoy, F., “Neural crest-derived dental stem cells--where we are and where we are going.” J Dent, 2014. 42(9): 1043-51. [112] Ellis, K.M., O'Carroll, D.C., Lewis, M.D., Rychkov, G.Y., and Koblar. S.A., “Neurogenic potential of dental pulp stem cells isolated from murine incisors.” Stem Cell Res Ther, 2014. 5(1): 30. [113] Chang, C.C., Chang, K.C., Tsai, S.J., Chang, H.H., and Lin, C.P., “Neurogenic differentiation of dental pulp stem cells to neuron-like cells in dopaminergic and motor neuronal inductive media.” J Formos Med Assoc, 2014. 113(12): 956-65. [114] Haratizadeh, S., Nazm Bojnordi, M., Darabi, S., Karimi, N., Naghikhani, M., Ghasemi Hamidabadi, H., and Seifi, M., “Condition medium of cerebrospinal fluid and retinoic acid induces the transdifferentiation of human dental pulp stem cells into neuroglia and neural like cells.” Anat Cell Biol, 2017. 50(2): 107-114. [115] Varga, G., and Gerber, G., “Mesenchymal stem cells of dental origin as promising tools for neuroregeneration.” Stem Cell Res Ther, 2014, 5(2): 61. [116] Yamamoto, A., Sakai, K., Matsubara, K., Kano, F., and Ueda, M., “Multifaceted neuro-regenerative activities of human dental pulp stem cells for functional recovery after spinal cord injury.” Neurosci Res, 2014. 78: 16-20. [117] Zhang, J., Lu, X., Feng, G., Gu, Z., Sun, Y., Bao, G., Xu, G., Lu, Y., Chen, J, Xu, L., Feng, X., and Cui, Z., “Chitosan scaffolds induce human dental pulp stem cells to neural differentiation: potential roles for spinal cord injury therapy.” Cell Tissue Res, 2016. 366(1): 129-42. [118] Yang, K.L., Chen, M.F., Liao, C.H., Pang, C.Y., and Lin, P.Y., “A simple and efficient method for generating Nurr1-positive neuronal stem cells from human wisdom teeth (tNSC) and the potential of tNSC for stroke therapy.” Cytotherapy, 2009. 11(5): 606-17. [119] Leong, W.K., Lewis, M.D., and Koblar, S.A., “Concise review: Preclinical studies on human cell-based therapy in rodent ischemic stroke models: where are we now after a decade?” Stem Cells, 2013. 31(6): 1040-3. [120] Fujii, H., Matsubara, K., Sakai, K., Ito, M., Ohno, K., Ueda, M., and Yamamoto, A., “Dopaminergic differentiation of stem cells from human deciduous teeth and their therapeutic benefits for Parkinsonian rats.” Brain Res, 2015. 1613: 59-72. [121] Wang, F., Jia, Y., Liu, J., Zhai, J., Cao, N., Yue, W., He, H., and Pei, X., “Dental pulp stem cells promote regeneration of damaged neuron cells on the cellular model of Alzheimer's disease.” Cell Biol Int, 2017. 41(6): 639-650. [122] Mead, B., Logan, A., Berry, M., Leadbeater, W., and Scheven, B.A., “Intravitreally transplanted dental pulp stem cells promote neuroprotection and axon regeneration of retinal ganglion cells after optic nerve injury.” Invest Ophthalmol Vis Sci, 2013. 54(12): 7544-56. [123] Mead, B., Hill, L.J., Blanch, R.J., Ward, K., Logan, A., Berry, M., Leadbeater, W., and Scheven B.A., “Mesenchymal stromal cell-mediated neuroprotection and functional preservation of retinal ganglion cells in a rodent model of glaucoma.” Cytotherapy, 2016. 18(4): 487-96. [124] Mead, B., Logan, A., Berry, M., Leadbeater, W., and Scheven, B.A., “Concise Review: Dental Pulp Stem Cells: A Novel Cell Therapy for Retinal and Central Nervous System Repair.” Stem Cells, 2017. 35(1): 61-67. [125] Grossniklaus, H.E., Geisert, E.E., and Nickerson, J.M., “Introduction to the Retina.” Prog Mol Biol Transl Sci, 2015. 134: 383-96. [126] Benowitz, L., and Yin, Y., “Rewiring the injured CNS: lessons from the optic nerve.” Exp Neurol, 2008. 209(2): 389-98. [127] You, Y., Gupta, V.K., Graham, S.L., and Klistorner, A., “Anterograde degeneration along the visual pathway after optic nerve injury.” PLoS One, 2012. 7(12): e52061. [128] Mansour-Robaey, S., Clarke, D.B., Wang, Y.C., Bray, G.M., and Aguayo, A.J., “Effects of ocular injury and administration of brain-derived neurotrophic factor on survival and regrowth of axotomized retinal ganglion cells.” Proc Natl Acad Sci USA, 1994. 91(5): 1632-6. [129] Monsul, N.T., Geisendorfer, A.R., Han, P.J., Banik, R., Pease, M.E., Skolasky, R.L. Jr., and Hoffman, P.N., “Intraocular injection of dibutyryl cyclic AMP promotes axon regeneration in rat optic nerve.” Exp Neurol, 2004. 186(2): 124-33. [130] Müller, A., Hauk, T.G., and Fischer, D., “Astrocyte-derived CNTF switches mature RGCs to a regenerative state following inflammatory stimulation.” Brain, 2007. 130(Pt 12): 3308-20. [131] Hauk, T.G., Leibinger, M., Müller, A., Andreadaki, A., Knippschild, U., and Fischer, D., “Stimulation of axon regeneration in the mature optic nerve by intravitreal application of the toll-like receptor 2 agonist Pam3Cys.” Invest Ophthalmol Vis Sci, 2010. 51(1): 459-64. [132] Dahlmann-Noor, A., Vijay, S., Jayaram, H., Limb, A., and Khaw, P.T., “Current approaches and future prospects for stem cell rescue and regeneration of the retina and optic nerve.” Can J Ophthalmol, 2010. 45(4): 333-41. [133] Parameswaran, S., Balasubramanian, S., Babai, N., Qiu, F., Eudy, J.D., Thoreson, W.B., and Ahmad, I., “Induced pluripotent stem cells generate both retinal ganglion cells and photoreceptors: therapeutic implications in degenerative changes in glaucoma and age-related macular degeneration.” Stem Cells, 2010. 28(4): 695-703. [134] Eiraku, M., Takata, N., Ishibashi, H., Kawada, M., Sakakura, E., Okuda, S., Sekiguchi, K., Adachi, T., and Sasai, Y., “Self-organizing optic-cup morphogenesis in three-dimensional culture.” Nature, 2011. 472(7341): 51-6. [135] Buchholz, D.E., Pennington, B.O., Croze, R.H., Hinman, C.R., Coffey, P.J., and Clegg, D.O., “Rapid and efficient directed differentiation of human pluripotent stem cells into retinal pigmented epithelium.” Stem Cells Transl Med, 2013. 2(5): 384-93. [136] Maruotti, J., Wahlin, K., Gorrell, D., Bhutto, I., Lutty, G., and Zack, D.J., “A simple and scalable process for the differentiation of retinal pigment epithelium from human pluripotent stem cells.” Stem Cells Transl Med, 2013. 2(5): 341-54. [137] Osakada, F., Ikeda, H., Mandai, M., Wataya, T., Watanabe, K., Yoshimura, N., Akaike, A., Sasai, Y., and Takahashi, M., “Toward the generation of rod and cone photoreceptors from mouse, monkey and human embryonic stem cells.” Nat Biotechnol, 2008. 26(2): 215-24. [138] Lamba, D.A., Gust, J., and Reh, T.A., “Transplantation of human embryonic stem cell-derived photoreceptors restores some visual function in Crx-deficient mice.” Cell Stem Cell, 2009. 4(1): 73-9. [139] Fang, I.M., Yang, C.M., Yang, C.H., Chiou, S.H., and Chen, M.S., “Transplantation of induced pluripotent stem cells without C-Myc attenuates retinal ischemia and reperfusion injury in rats.” Exp Eye Res, 2013. 113: 49-59. [140] Johnson, T.V., DeKorver, N.W., Levasseur, V.A., Osborne, A., Tassoni, A., Lorber, B., Heller, J.P., Villasmil, R., Bull, N.D., Martin, K.R., and Tomarev, S.I., “Identification of retinal ganglion cell neuroprotection conferred by platelet-derived growth factor through analysis of the mesenchymal stem cell secretome.” Brain, 2014. 137(Pt 2): 503-19. [141] Satarian, L., Javan, M., Kiani, S., Hajikaram, M., Mirnajafi-Zadeh, J., and Baharvand, H., “Engrafted human induced pluripotent stem cell-derived anterior specified neural progenitors protect the rat crushed optic nerve.” PLoS One, 2013. 8(8): e71855. [142] Aizawa, Y. and Shoichet, M.S., “The role of endothelial cells in the retinal stem and progenitor cell niche within a 3D engineered hydrogel matrix.” Biomaterials, 2012. 33(21): 5198-205. [143] Kuo, Y.C. and Huang, M.J., “Material-driven differentiation of induced pluripotent stem cells in neuron growth factor-grafted poly(ε-caprolactone)- poly(β-hydroxybutyrate) scaffolds.” Biomaterials, 2012. 33(23): 5672-82. [144] Moore, K.A. and Lemischka, I.R., “Stem cells and their niches.” Science, 2006. 311(5769): 1880-5. [145] Meldrum, B.S., “Glutamate as a neurotransmitter in the brain: review of physiology and pathology.” J Nutr, 2000. 130(4S Suppl): 1007S-15S. [146] Okubo, Y., Sekiya, H., Namiki, S., Sakamoto, H., Iinuma, S., Yamasaki, M., Watanabe, M., Hirose, K., and Iino, M. “Imaging extrasynaptic glutamate dynamics in the brain.” Proc Natl Acad Sci USA, 2010. 107(14): 6526-31. [147] Mattson, M.P., “Glutamate and neurotrophic factors in neuronal plasticity and disease.” Ann N Y Acad Sci, 2008. 1144: 97-112. [148] Huang, H.C., Chang, P.Y., Chang, K., Chen, C.Y., Lin, C.W., Chen, J.H., Mou, C.Y., Chang, Z.F., and Chang, F.H., “Formulation of novel lipid-coated magnetic nanoparticles as the probe for in vivo imaging.” J Biomed Sci, 2009. 16: 86. [149] Chen, M.H., Hsu, Y.H., Lin, C.P., Chen, Y.J., and Young. T.H., “Interactions of acinar cells on biomaterials with various surface properties.” J Biomed Mater Res A, 2005. 74(2): 254-62. [150] Lin, C.Y., Chang, F.H., Chen, C.Y., Huang, C.Y., Hu, F.C., Huang, W.K., Ju, S.S., and Chen, M.H., “Cell therapy for salivary gland regeneration.” J Dent Res, 2011. 90(3): 341-6. [151] Goldberg, J.L., Espinosa, J.S., Xu, Y., Davidson, N., Kovacs, G.T., and Barres, B.A., “Retinal ganglion cells do not extend axons by default: promotion by neurotrophic signaling and electrical activity.” Neuron, 2002. 33(5): 689-702. [152] Chang, M.F., Sun, C.Y., Chen, C.J., and Chang, S.C., “Functional motifs of delta antigen essential for RNA binding and replication of hepatitis delta virus.” J Virol, 1993. 67(5): 2529-36. [153] Tseng, L.T., Lin, C.L., Tzen, K.Y., Chang, S.C., and Chang, M.F., “LMBD1 protein serves as a specific adaptor for insulin receptor internalization.” J Biol Chem, 2013. 288(45): 32424-32. [154] Gregory, C.A., Gunn, W.G., Peister, A., and Prockop, D.J., “An Alizarin red-based assay of mineralization by adherent cells in culture: comparison with cetylpyridinium chloride extraction.” Anal Biochem, 2004. 329(1): 77-84. [155] Chen, R.S., Chen, M.H., and Young, T.H., “Induction of differentiation and mineralization in rat tooth germ cells on PVA through inhibition of ERK1/2.” Biomaterials, 2009. 30(4): 541-7. [156] Liu, Y., Xu, X., Tang, R., Chen, G., Lei, X., Gao, L., Li, W., and Chen, Y., “Viability of primary cultured retinal neurons in a hyperglycemic condition.” Neural Regen Res, 2013. 8(5): 410-9. [157] Boulaiz H., Alvarez P.J., Ramirez A., Marchal J.A., Prados J., Rodríguez-Serrano F., Perán M., Melguizo C., and Aranega A., “Nanomedicine: application areas and development prospects.” Int J Mol Sci, 2011. 12(5): 3303-21. [158] Karjoo, Z., McCarthy, H.O., Patel, P., Nouri, F.S., and Hatefi, A., “Systematic engineering of uniform, highly efficient, targeted and shielded viral-mimetic nanoparticles.” Small, 2013. 9(16): 2774-83. [159] Fuchs, S.M., and Raines, R.T., “Internalization of cationic peptides: the road less (or more?) traveled.” Cell Mol Life Sci, 2006. 63(16): 1819-22. [160] Ye, J., Liu, E., Yu, Z., Pei, X., Chen, S., Zhang, P., Shin, M.C., Gong, J., He, H., and Yang, V.C., “CPP-Assisted Intracellular Drug Delivery, What Is Next?” Int J Mol Sci, 2016. 17(11) pii: E1892. [161] Kukowska-Latallo, J.F., Bielinska, A.U., Johnson, J., Spindler, R., Tomalia, D.A., and Baker, J.R. Jr., “Efficient transfer of genetic material into mammalian cells using Starburst polyamidoamine dendrimers.” Proc Natl Acad Sci USA, 1996. 93(10): 4897-902. [162] Yang, J.P., and Huang, L., “Overcoming the inhibitory effect of serum on lipofection by increasing the charge ratio of cationic liposome to DNA.” Gene Ther, 1997. 4(9): 950-60. [163] Lee, C.H., Ni, Y.H., Chen, C.C., Chou, C., and Chang, F.H., “Synergistic effect of polyethylenimine and cationic liposomes in nucleic acid delivery to human cancer cells.” Biochim Biophys Acta, 2003. 1611(1-2): 55-62. [164] Vinogradov, S.V., Bronich, T.K., and Kabanov, A.V., “Nanosized cationic hydrogels for drug delivery: preparation, properties and interactions with cells.” Adv Drug Deliv Rev, 2002. 54(1): 135-47. [165] Khalil, I.A., Kogure, K., Akita, H., and Harashima, H., “Uptake pathways and subsequent intracellular trafficking in nonviral gene delivery.” Pharmacol Rev, 2006. 58(1): 32-45. [166] Abbas, A.O., Donovan, M.D., and Salem, A.K., “Formulating poly(lactide-co-glycolide) particles for plasmid DNA delivery.” J Pharm Sci, 2008. 97(7): 2448-61. [167] Audouy, S., Molema, G., de Leij, L., and Hoekstra, D., “Serum as a modulator of lipoplex-mediated gene transfection: dependence of amphiphile, cell type and complex stability.” J Gene Med, 2000. 2(6): 465-76. [168] Wightman, L., Kircheis, R., Rössler, V., Carotta, S., Ruzicka, R., Kursa, M., and Wagner, E., “Different behavior of branched and linear polyethylenimine for gene delivery in vitro and in vivo.” J Gene Med, 2001. 3(4): 362-72. [169] Polo, L., Valduga, G., Jori, G., and Reddi, E., “Low-density lipoprotein receptors in the uptake of tumour photosensitizers by human and rat transformed fibroblasts.” Int J Biochem Cell Biol, 2002. 34(1): 10-23. [170] Pichon, C., Billiet, L., and Midoux, P., “Chemical vectors for gene delivery: uptake and intracellular trafficking.” Curr Opin Biotechnol, 2010. 21(5): 640-5. [171] Behrens, S., and Appel, I., “Magnetic nanocomposites.” Curr Opin Biotechnol, 2016. 39: 89-96. [172] Cheng, K.W., and Hsu, S.H., “A facile method to prepare superparamagnetic iron oxide and hydrophobic drug-encapsulated biodegradable polyurethane nanoparticles.” Int J Nanomedicine, 2017. 12: 1775-1789. [173] Nobuto, H., Sugita, T., Kubo, T., Shimose, S., Yasunaga, Y., Murakami, T., and Ochi, M., “Evaluation of systemic chemotherapy with magnetic liposomal doxorubicin and a dipole external electromagnet.” Int J Cancer, 2004. 109(4): 627-35. [174] Satarkar, N.S., and Hilt, J.Z., “Magnetic hydrogel nanocomposites for remote controlled pulsatile drug release.” J Control Release, 2008. 130(3): 246-51. [175] Pradhan, P., Giri, J., Rieken, F., Koch, C., Mykhaylyk, O., Döblinger, M., Banerjee, R., Bahadur, D., and Plank, C., “Targeted temperature sensitive magnetic liposomes for thermo-chemotherapy.” J Control Release, 2010. 142(1): 108-21. [176] Fire, A., Xu, S., Montgomery, M.K., Kostas, S.A., Driver, S.E., and Mello, C.C., “Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.” Nature, 1998. 391(6669): 806-11. [177] Li, S., Hu, J., Zhang, G., Qi, W., Zhang, P., Li, P., Zeng, Y., Zhao, W., and Tan, Y., “Extracellular Ca2+ Promotes Odontoblastic Differentiation of Dental Pulp Stem Cells via BMP2-Mediated Smad1/5/8 and Erk1/2 Pathways.” J Cell Physiol, 2015. 230(9): 2164-73. [178] Wang, Y., Xu, C., and Ow, H., “Commercial nanoparticles for stem cell labeling and tracking.” Theranostics, 2013. 3(8): 544-60. [179] Huang, X., Zhang, F., Wang, Y., Sun, X., Choi, K.Y., Liu, D., Choi, J.S., Shin, T.H., Cheon, J., Niu, G., and Chen, X., “Design considerations of iron-based nanoclusters for noninvasive tracking of mesenchymal stem cell homing.” ACS Nano, 2014. 8(5): 4403-14. [180] Mulder, W.J., Strijkers, G.J., van Tilborg, G.A., Griffioen, A.W., and Nicolay, K., “Lipid-based nanoparticles for contrast-enhanced MRI and molecular imaging.” NMR Biomed, 2006. 19(1): 142-64. [181] Mason, C., and Dunnill, P., “A brief definition of regenerative medicine.” Regen Med, 2008. 3(1): 1-5. [182] Jain, A., and Bansal, R., “Applications of regenerative medicine in organ transplantation.” J Pharm Bioallied Sci, 2015. 7(3): 188-94. [183] Stenudd, M., Sabelström, H., and Frisén, J., “Role of endogenous neural stem cells in spinal cord injury and repair.” JAMA Neurol, 2015. 72(2): 235-7. [184] Grégoire, C.A., Goldenstein, B.L., Floriddia, E.M., Barnabé-Heider, F., and Fernandes, K.J., “Endogenous neural stem cell responses to stroke and spinal cord injury.” Glia, 2015. 63(8): 1469-82. [185] Wohl, S.G., Schmeer, C.W., and Isenmann, S. “Neurogenic potential of stem/progenitor-like cells in the adult mammalian eye.” Prog Retin Eye Res, 2012. 31(3): 213-42. [186] Cho, K.S., Yang, L., Lu, B., Feng Ma, H., Huang, X., Pekny, M., and Chen, D.F., “Re-establishing the regenerative potential of central nervous system axons in postnatal mice.” J Cell Sci, 2005. 118(Pt 5): 863-72. [187] Lehmann, M., Fournier, A., Selles-Navarro, I., Dergham, P., Sebok, A., Leclerc, N., Tigyi, G., and McKerracher, L., “Inactivation of Rho signaling pathway promotes CNS axon regeneration.” J Neurosci, 1999. 19(17): 7537-47. [188] Koprivica, V., Cho, K.S., Park, J.B., Yiu, G., Atwal, J., Gore, B., Kim, J.A., Lin, E., Tessier-Lavigne, M., Chen, D.F., and He, Z., “EGFR activation mediates inhibition of axon regeneration by myelin and chondroitin sulfate proteoglycans.” Science, 2005. 310(5745): 106-10. [189] Gautam, V., Naureen, S., Shahid, N., Gao, Q., Wang, Y., Nisbet, D., Jagadish, C., and Daria, V.R., “Engineering Highly Interconnected Neuronal Networks on Nanowire Scaffolds.” Nano Lett, 2017. 17(6): 3369-3375. | - |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/79041 | - |
dc.description.abstract | 再生醫學(regenerative medicine)是結合生物學、材料科學、工程學和醫學等學科以刺激病患自體組織器官再生,或是製造出具有功能性的類器官(organoids),利用移植來修復或替換體內因外傷、疾病或老化而受損的細胞、組織甚至器官。 組織工程(tissue engineering)是實現再生醫學的一種方法,藉由細胞、生醫材料(biomaterials)及適當的生長訊息(signals)相互調控,以促進生物的再生能力。其中,利用生醫材料製成載體(carrier)或支架(scaffold)來調控基因、生長因子及其他能與細胞作用之物質,得以探討幹細胞分化以及組織再生過程之分子作用機制與其未來的醫療應用。
本論文共分成兩個部分進行研究,主要以不同的新穎性生醫材料來進行組織再生,以探討其於再生醫學上的應用。第一部分是利用一種帶正電之膽固醇製成的磁性奈米載體:GCC-Fe3O4,遞送小分子核糖核酸-218(microRNA-218,miR-218)及其抑制劑,以刺激人類牙髓幹細胞(human dental pulp stem cells,hDPSCs)礦化(mineralization)。此奈米載體具有極高的遞送效率,並對細胞不產生明顯的細胞毒性(cytotoxicity),即使在含有血清的培養條件中也能展現出優異的遞送效果。研究結果顯示,小分子核糖核酸-218具有負向調控牙齒礦化之功能,藉由抑制小分子核糖核酸-218,牙髓幹細胞之礦化現象明顯增加。此現象也被證實與MAPK/ ERK(mitogen-activated protein kinases/ extracellular signal-regulated kinases)之訊息傳導路徑有關。由於GCC-Fe3O4奈米載體中含氧化鐵並帶有磁性,未來可進一步利用磁力進行操控,並能以非侵入式顯影系統做即時性的體內追蹤,無論是在生醫研究領域或臨床治療上都能提供更廣泛的應用性。 成人的神經系統再生能力有限,一旦受損,往往是永久而難以復原的。在牙齒幹細胞的分化研究中,我們曾經嘗試以誘導神經分化的培養基(neurodifferentiation medium)進行幹細胞之神經性分化,但其結果並不理想。因此,第二部分的研究專注於神經組織的再生,利用幹細胞及生物相容性支架,進行組織工程,以創造出視網膜神經節細胞(retinal ganglion cells,RGCs)最佳的生長環境。此部分利用聚谷氨酸苄酯(poly-γ-benzyl-L-glutamate,PBG)製成三維立體支架(3D scaffold),將自人類胚胎幹細胞(human embryonic stem cells,hESCs)誘導分化成的視網膜神經節前驅細胞(retinal ganglion cell progenitors,RGCPs)、視網膜神經節細胞及視網膜組織分別培養於其中,以觀察其神經生長與再生之情形。研究結果發現,PBG支架相較於組織工程中常見的聚己內酯(polycaprolactone,PCL)支架,不但能支持神經細胞的生存,更能顯著的促進神經纖維生長(neurite outgrowth)。藉由視神經的重建,不但能讓視覺退化之病患得以重見光明,並為同樣難以修復之中樞神經系統創造再生的可能性。 整體而言,藉由開發新穎性的生醫材料進行組織工程,以促進再生醫學之各種應用,不論是在牙齒再生或神經的重建,都具有極大的臨床實用價值。若能有效減輕病患的痛苦,並改善其生活品質,對病患自身及全體人類的健康都將是一大福祉。 | zh_TW |
dc.description.abstract | Regenerative medicine combines biology, materials science, engineering, and medicine to replace or regenerate cells, tissues or organs for restoring or establishing their normal functions. It can be achieved by stimulating autologous repair mechanisms for self-regeneration or creating functional organoids for transplantations to repair or replace damaged cells, tissues, or even organs that caused from trauma, diseases or aging in the body. Tissue engineering is considered as one of the major approaches for regenerative medicine, which combining cells, biomaterials, and appropriate signals to promote the regeneration abilities of organisms. Novel biomaterials can be made into carriers or scaffolds to manipulate genes, growth factors and many other cell-reacting substances for exploring the mechanisms of stem cell differentiation and tissue regeneration as well as developing future medical applications.
This dissertation includes two parts of research by exploiting different biomaterials for tissue regeneration as applications in regenerative medicine. In the first part, a cationic cholesterol-based magnetic nanocarrier: GCC-Fe3O4 (GCC-Fe) was developed to deliver microRNA-218 (miR-218) and miR-218 inhibitor for promoting mineralization potentials of human dental pulp stem cells (hDPSCs). Results showed that this nanocarrier had an extremely high transfection efficiency with insignificant cytotoxicity. Furthermore, the transfection efficiency was not disturbed under serum-containing culture conditions. On the other hand, it showed that miR-218 had a negative regulation role in hDPSCs mineralization. By inhibiting miR-218, the mineralization degree of hDPSCs was increased significantly. It was also confirmed that this induced mineralization was related to the mitogen-activated protein kinases/ extracellular signal-regulated kinases (MAPK/ ERK) pathway. Since GCC-Fe contains superparamagnetic iron oxide (SPIO) nanoparticles, it can be further manipulated by magnetic force, and tracked by non-invasive imaging systems in vivo. GCC-Fe has great potentials to be widely applied in biomedical researches as well as clinical applications. The regeneration ability of adult nervous system is limited. Once impaired, often lead to permanent damages which were nearly impossible to recover. In the previous study of stem cell differentiation, the attempt of using neurodifferentiation medium for human dental stem cells induction was not satisfying. Therefore, the second part of this dissertation focused on nerve regeneration. In this study, three-dimensional (3D) scaffolds of poly-γ-benzyl-L-glutamate (PBG) and polycaprolactone (PCL) were designed with different alignments. The survival and regeneration abilities of human embryonic stem cell (hESC)-derived retinal ganglion cell progenitors (RGCPs), primary retinal ganglion cells (RGCs), and retinal explants were used for evaluation respectively. Results indicated that PBG scaffolds could support the survival of neurons and promote long and robust neurite outgrowth compared to PCL scaffolds. Through this tissue engineering approach, not only the optic nerve can be reconstructed for vision impaired patients to restore their vision; but also the optic nerve can be served as a model for studying regeneration of the central nervous system (CNS). In conclusion, developing novel biomaterials to fulfill all kinds of applications in regenerative medicine is crucial. No matter for tooth regeneration or nerve reconstruction, they all have great values in clinical practice to alleviate the suffering of patients and improve their quality of life. With the progress of tissue engineering, it will further promote the health of individuals as well as the well-being for all. | en |
dc.description.provenance | Made available in DSpace on 2021-07-11T15:38:59Z (GMT). No. of bitstreams: 1 ntu-107-D00422003-1.pdf: 5562632 bytes, checksum: 7bad5f3df5dab0dfa396fdd7c413b0a4 (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | 口試委員會審定書……………………………………………………………………i
誌謝………………………………………………………………………………………………ii 中文摘要………………………………………………………………………………………iv ABSTRACT……………………………………………………………………………………vi CONTENTS……………………………………………………………………………………ix LIST OF FIGURES…………………………………………………………………xiii ABBRVIATIONS…………………………………………………………………………xiv CHAPTER 1. INTRODUCTION………………………………………………1 1.1 Nanomedicine.………………………………………………………1 1.2 Biomaterials for biomedical applications……………………………………………….…….1 1.2.1 Nanomaterials……………….……………………………………………...………...2 1.2.2 Nanocarriers………………………………………………………………………….3 1.2.3 Biocompatible scaffolds……………………………………………………………...5 1.3 Gene delivery……………………………………………………………………….………...6 1.4 Tissue engineering……………………………………………………………….…………...7 1.5 Dental stem cells in regenerative medicine……………………………………….…………..9 1.5.1 Discovery and characteristics of dental stem cells……………………....…………….9 1.5.2 Differentiation of dental stem cells by microRNAs regulation………………….…...10 1.5.3 Dental pulp stem cells for neuroregeneration……….……………………………….11 1.6 Optic nerve regeneration and visual reconstruction…………………………………………13 CHAPTER 2. MOTIVATION AND SPECIFIC AIMS………………………………………...16 CHAPTER 3. EXPERIMENTAL FLOWCHARTS…………………………………………...17 CHAPTER 4. MATERIALS AND METHODS………………………………………………..18 4.1 Materials………………………………………………………………………………….18 4.2 Methods…………………………………………………………………………………..19 4.2.1 Preparation of the magnetic GCC-Fe3O4 nanocarrier……………...………….19 4.2.1.1 Synthesis of the GEC-Chol lipid………………………………………….19 4.2.1.2 Preparation of the magnetic GCC-Fe3O4 nanocarrier……………………20 4.2.2 Size and zeta potential measurements………………………………………….20 4.2.3 Scaffold preparation and coating……………………………………………….21 4.2.4 Cell cultures……………………………………………………………………22 4.2.4.1 Cell lines………………………………………………………………….22 4.2.4.2 Isolation and culture of primary rat salivary gland acinar cells……………22 4.2.4.3 Isolation and culture of primary mouse retinal ganglion cells…………….22 4.2.4.4 Isolation and culture of primary human dental pulp cells…………………23 4.2.4.5 Other stem cells…………………………………………………………...24 4.2.5 Retinal explant culture…………………………………………………………25 4.2.6 Transfection efficiency of the GCC-Fe3O4 nanocarrier………………………...25 4.2.6.1 Transfection preparations…………………………………………………25 4.2.6.2 Efficiency evaluation……………………………………………………..25 4.2.6.3 Monitoring the nucleic acids after transfection…………………………...26 4.2.7 Functional delivery experiments……………………………………………….27 4.2.7.1 The c-myc ODN…………………………………………………………..27 4.2.7.2 siRNA…………………………………………………………………….27 4.2.7.3 miRNA…………………………………………………………………...27 4.2.8 Mineralization assay…………………………………………………………...28 4.2.9 Western blot analysis…………………………………………………………...28 4.2.10 Cell viability analysis…………………………………………………………29 4.2.11 Immunohistochemistry……………………………………………………….30 4.2.12 Scanning electron microscope imaging……………………………………….30 4.2.13 Statistical analysis…………………………………………………………….31 CHAPTER 5. RESULTS………………………………………………………………………...32 PART I: Promoting dentinogenesis of hDPSCs through inhibiting miR-218 by using magnetic nanocarrier delivery…………………………………………………………………….32 5.1.1 The cationic core-shell lipid nanocarrier……………………………………….32 5.1.2 High transfection efficiency of GCC-Fe with serum compatibility…………….33 5.1.3 Efficient magnetic nanocarrier delivery for hDPSCs…………………………..36 5.1.4 Promoting mineralization of hDPSCs by miR-218 inhibition………………….37 5.1.5 Effects of induction and miR-218 inhibition were related to the ERK1/2 pathway………………………………………………………………………...38 PART II: A bioengineering approach for promoting retina ganglion cell survival and optic nerve regeneration……………………………………………………………………………..39 5.2.1 The scaffold that designed for nerve regeneration……………………………...39 5.2.2 Robust neurite outgrowth of RGCPs on PBG scaffolds………………………...40 5.2.3 PBG scaffolds supported survival and promoted neurites outgrowth of RGCs...40 5.2.4 PBG scaffolds promoted extremely long and robust neurites from retinal explants …………………………………………………………………………………42 CHAPTER 6. DISCUSSION……………………………………………………………………43 CHAPTER 7. FIGURES AND TABLES………………………………………………………..52 CHAPTER 8. APPENDIXES…………………………………………………………………...62 CHAPTER 9. REFERENCES…………………………………………………………………..68 | - |
dc.language.iso | en | - |
dc.title | 利用新穎性生醫材料於再生醫學的應用:在牙組織再生及神經重建之研究 | zh_TW |
dc.title | Application of Novel Biomaterials for Regenerative Medicine: A Study for Dental Tissue Regeneration and Optic Nerve Reconstruction | en |
dc.type | Thesis | - |
dc.date.schoolyear | 106-2 | - |
dc.description.degree | 博士 | - |
dc.contributor.oralexamcommittee | 張明富;詹迺立;陳羿貞;周涵怡 | zh_TW |
dc.contributor.oralexamcommittee | ;;; | en |
dc.subject.keyword | 再生醫學,組織工程,奈米載體,三維立體支架,小分子核糖核酸,牙髓幹細胞,礦化,視神經,視網膜神經節細胞,神經纖維生長, | zh_TW |
dc.subject.keyword | regenerative medicine,tissue engineering,nanocarrier,3D scaffold,microRNA,dental pulp stem cell,mineralization,optic nerve,retinal ganglion cell,neurite outgrowth, | en |
dc.relation.page | 90 | - |
dc.identifier.doi | 10.6342/NTU201803303 | - |
dc.rights.note | 未授權 | - |
dc.date.accepted | 2018-08-14 | - |
dc.contributor.author-college | 醫學院 | - |
dc.contributor.author-dept | 臨床牙醫學研究所 | - |
dc.date.embargo-lift | 2023-10-11 | - |
顯示於系所單位: | 臨床牙醫學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-106-2.pdf 目前未授權公開取用 | 5.43 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。