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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 詹世鵬 | zh_TW |
| dc.contributor.author | 王于箴 | zh_TW |
| dc.contributor.author | Yu-Chen Wang | en |
| dc.date.accessioned | 2021-07-11T15:30:31Z | - |
| dc.date.available | 2024-02-28 | - |
| dc.date.copyright | 2018-10-09 | - |
| dc.date.issued | 2018 | - |
| dc.date.submitted | 2002-01-01 | - |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78936 | - |
| dc.description.abstract | 粒線體融合和分裂現象的不平衡參與調控癌症生成，Non-Metastatic 4 (NME4)是位在粒線體的核苷酸二磷酸激酶，其主要參與在粒線體融合的過程中。有趣的是，我們先前的結果顯示在人類細胞中，腫瘤抑制子let-7 miRNA可能調控NME4，使我們想尋找let-7 miRNA與粒線體的關聯性以及其生理上的意義。由於以線蟲研究let-7的功能和表型有許多優點，本篇研究使用線蟲作為模式生物，測定線蟲中NME4同源基因ndk-1是否會受到let-7調控。而我們也確實發現在線蟲中降低ndk-1基因表現會像在人類細胞中降低NME4一樣造成粒線體碎片化，且在mid L4階段的let-7(n2853)線蟲中，碎片化粒線體的比例也顯著下降，我們的實驗結果指出let-7可能影響粒線體型態，而我們的研究也是第一個嘗試探討let-7和粒線體型態功能上的關聯性，這有助於發現新的miRNA對於調控粒線體動態平衡的路徑。 | zh_TW |
| dc.description.abstract | Imbalance of mitochondrial fission and fusion activities has been implicated in carcinogenesis. NME4, a mitochondria-localized nucleoside diphosphate kinase, is involved in mitochondrial fusion process. Interestingly, our previous results showed that the tumor suppressor miRNA let-7 seems to regulate NME4 in human cells, inspiring us to seek possible functional connection for let-7 miRNA to mitochondrial and the biological implication for this connection. In this study, we chose C. elegans as the model organism due to its advantages in studying let-7 function and phenotypes. We seek to determine whether the NME4 homolog ndk-1 in C. elegans is also regulated by let-7. Indeed, like NME4 in human cells, knockdown of ndk-1 in C. elegans caused mitochondrial fragmentation. In addition, we found that fragmentation of mitochondria is significantly reduced in let-7(n2853) mutants at the mid L4 stage. These results suggest that let-7 may play a role in mitochondrial morphology. Our study is the first attempt to examine function relevance of let-7 to mitochondria morphology and will help discover novel pathways for miRNAs to control mitochondrial dynamics. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-11T15:30:31Z (GMT). No. of bitstreams: 1 ntu-107-R05445124-1.pdf: 5918100 bytes, checksum: da1b8a783e1af5581c7b50fa150be490 (MD5) Previous issue date: 2018 | en |
| dc.description.tableofcontents | 誌謝 I
摘要 II Abstract III Chapter 1 Introduction 1 The let-7 miRNA 1 The role of the let-7 miRNA in C. elegans development 1 The let-7 miRNA is evolutionarily conserved 1 The let-7 miRNA is regarded as a tumor suppressor 2 NME4 may be a novel let-7 target gene 2 NME4 3 The NME family 3 The role of NME4 in mitochondrial fusion 3 Relevance of NME4 and mitochondrial dynamics in cancer 3 Association of NME4 and the let-7 miRNA 4 The C. elegans NME4 homolog ndk-1 4 Mitochondria-associated NDPKs across species 5 Project proposal 5 Chapter 2 Materials and methods 6 E. coli strains 6 C. elegans strains 6 C. elegans culture conditions 7 Synchronous growth 7 RNA interference (RNAi) 7 Slide preparation 8 Genomic DNA extraction 8 RNA extraction 9 Quantitative real-time RT-PCR 10 DNase treatment 10 cDNA synthesis 10 qRT-PCR 11 Construction of plasmids 12 Mitochondrial staining 15 Mitochondrial imaging 15 Mitochondrial morphologies analysis 15 Microinjection 16 Strain crosses 17 Single worm PCR 17 Detection of let-7(n2853) point mutation 18 Chapter 3 Results 19 C. elegans NDK-1 may function similarly to human NME4 that participates in mitochondrial fusion. 19 The let-7 miRNA plays a role in mitochondria dynamics at the mid L4 stage. 21 Mitochondrial dynamic seems to maintain balance in vulva in let-7(n2853) at the mid L4 stage. 22 Chapter 4 Discussion 24 Figures 26 Figure 1 26 Figure 2 29 Figure 3 33 Figure 4 37 References 39 Appendix 44 Appendix 1 44 Appendix 2 45 Appendix 3 47 Appendix 4 48 Appendix 5 51 Appendix 6 54 Appendix 7 56 Appendix 8 58 Appendix 9 59 Appendix 10 60 | - |
| dc.language.iso | en | - |
| dc.subject | let-7微小核醣核酸 | zh_TW |
| dc.subject | NDK-1 | zh_TW |
| dc.subject | NME4 | zh_TW |
| dc.subject | 核?酸二磷酸激? | zh_TW |
| dc.subject | NDK-1 | en |
| dc.subject | let-7 miRNA | en |
| dc.subject | nucleoside diphosphate kinase | en |
| dc.subject | NME4 | en |
| dc.title | 測定在線蟲中NME4同源基因ndk-1是否受到let-7微小核糖核酸調控 | zh_TW |
| dc.title | Determine whether the C. elegans NME4 homolog ndk-1 is regulated by the let-7 miRNA | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 106-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 潘俊良;羅時成 | zh_TW |
| dc.contributor.oralexamcommittee | ;; | en |
| dc.subject.keyword | let-7微小核醣核酸,核?酸二磷酸激?,NME4,NDK-1, | zh_TW |
| dc.subject.keyword | let-7 miRNA,nucleoside diphosphate kinase,NME4,NDK-1, | en |
| dc.relation.page | 60 | - |
| dc.identifier.doi | 10.6342/NTU201803824 | - |
| dc.rights.note | 未授權 | - |
| dc.date.accepted | 2018-08-17 | - |
| dc.contributor.author-college | 醫學院 | - |
| dc.contributor.author-dept | 微生物學研究所 | - |
| dc.date.embargo-lift | 2023-08-17 | - |
| Appears in Collections: | 微生物學科所 | |
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| ntu-106-2.pdf Restricted Access | 5.78 MB | Adobe PDF |
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