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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 劉啟德(Chi-Te Liu) | |
| dc.contributor.author | Meng-Wei Shen | en |
| dc.contributor.author | 沈孟薇 | zh_TW |
| dc.date.accessioned | 2021-05-19T17:57:33Z | - |
| dc.date.available | 2021-08-30 | |
| dc.date.available | 2021-05-19T17:57:33Z | - |
| dc.date.copyright | 2016-08-30 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-08-14 | |
| dc.identifier.citation | Adesemoye, A.O. and Kloepper, J.W. (2009) Plant-microbes interactions in enhanced fertilizer-use efficiency. Appl Microbiol Biotechnol 85: 1-12.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7892 | - |
| dc.description.abstract | Rhodopseudomonas palustris NTUIOB-PS3為分離自台灣水田土壤的光合菌株，不僅能促進各種作物生長，亦能有效降低植體內硝酸鹽的累積。本研究之目的為探討PS3光合菌促進宿主植物生長的機制。藉由分析植物的氮素吸收效率、同化酵素活性、氮素轉運蛋白之基因表現、二氧化碳固定效率等，闡明根圈微生物對於植物生理活性之影響。將PS3光合菌接種至水耕栽培系統，促使丸葉山東小白菜的地上部與根部鮮重顯著高於未接種的控制組。因PS3處理組之根部硝酸鹽轉運蛋白基因BjNRT1.1的轉錄活性與植體內的總氮含量皆比控制組來得高，推測PS3菌株可以提昇植物吸收氮素的效能。進一步計算其氮素吸收效率 (nitrogen uptake efficiency, NupE)，發現比控制組增加約40%。此外，接種PS3的植株其生長初期的光合作用效率較高，葉面積增加了約25 %，總碳含量也提昇了70%以上。綜合上述結果推測PS3 光合菌藉由植微交互作用提昇了宿主植物的氮與碳的代謝效率，因而增加生物質量的累積。 | zh_TW |
| dc.description.abstract | Rhodopseudomonas palustris NTUIOB-PS3, a phototrophic purple non-sulfur bacterium, was originally isolated from paddy field in Taiwan. It can not only improve plant growth, but also reduce the nitrate accumulation in plants. The aim of this study was to investigate the mechanism of its plant growth promotion effect. We would like to elucidate the effects of rhizosphere bacteria on plant physiology through evaluating the nitrogen uptake efficiency, activity of nitrogen assimilation associative enzymes, gene expression of nitrate transporters, and CO2 assimilation rate of plants. When hydroponic solution was supplemented with the PS3 inoculant, plant growth (fresh weight of shoots and roots) of Chinese cabbage was greater than that without inoculation (control group). Since the gene expression of the nitrate transporter BjNRT1.1 in the roots and total amounts of N in the plants were dramatically elevated, we deduced that PS3 could stimulate the N uptake efficiency (NupE) of plants. The N uptake efficiency was around 40% increase in comparison with that in the control group. On the other hand, the plants inoculated with PS3 showed higher photosynthetic rate in the early stage of plants, then the leave sizes and total C amounts in shoot were increased nearly 25 % and 70%, respectively. Taken together, we deduced that PS3 improves both N and C metabolic efficiencies of plants through plant-microbe interaction, as a result of higher biomass accumulation. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-19T17:57:33Z (GMT). No. of bitstreams: 1 ntu-105-R03b22038-1.pdf: 7000612 bytes, checksum: 4eb24603a5d7f8669b267da5782a913a (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 致謝 ii
中文摘要 iv Abstract v 目錄 vii 圖目錄 x 表目錄 xii List of abbreviations xiii 1. Introduction 1 2. Materials and methods 11 2-1 Plant materials and growth conditions 11 2-2 Preparation of bacterial inoculants 15 2-3 Measurement of nitrate contents in roots and leaves 15 2-4 Uptake of nitrate and ammonium 16 2-5 Measurement of dry weight, total N and C contents in plants 17 2-6 Determination of nitrogen assimilating enzyme activities 19 2-7 Measurement of soluble proteins 20 2-8 Determination of total chlorophyll 20 2-9 Measurement of leaf photosynthetic CO2 exchange 21 2-10 Total RNA extraction and gene expression analyses by quantitative real-time PCR (qPCR) 22 2-11 Statistical analysis 23 3. Results 24 3-1 Effects of R. palustris inoculants on plant growth 24 3-2 Effects of PS3 and C3 inoculants on the absolute growth rate (AGR) and relative growth rate (RGR) of Chinese cabbage 27 3-3 Nitrogen uptake 28 3-3-1 Lateral root development was highly stimulated by PS3 inoculant 28 3-3-2 PS3 inoculant enhanced nitrogen uptake efficiency of plants 30 3-3-3 Concentrations of nitrate in hydroponic nutrient solutions 30 3-3-4 Transcriptomic analyses of nitrogen metabolism-related genes 32 Nitrogen assimilation 35 3-4-1 PS3 inoculation dramatically reduced nitrate contents in mature plants 35 3-4-2 Activities of nitrogen assimilation related enzymes and soluble protein contents 36 3-5 Effects of PS3 inoculant on N use efficiency, N uptake efficiency and N utilization efficiency 39 3-6 Carbon assimilation 41 3-6-1 Effects of R. palustris inoculants on chlorophyll contents of Chinese cabbage. 41 3-6-2 Effects of R. palustris inoculants on C contents of Chinese cabbage 42 3-6-3 Effects of R. palustris inoculants on gas exchange efficiencies of Chinese cabbage 43 3-6-4 Effects of R. palustris inoculants on RuBisCO contents of Chinese cabbage 45 3-7 C:N ratio of Chinese cabbage 49 3-8 Graphical summary 51 4. Discussion 52 5. Conclusion and future prospects 62 6. Reference 64 圖目錄 Figure 1-1 Nitrogen and carbon metabolism in plant growth and development. 9 Figure 1-2 Scheme of this study. 10 Figure 2-1 Chinese cabbage grown in the hydroponic system. 13 Figure 2-2 Leaf age classification used in this study at 8 DAP. 13 Figure 2-3 Leaf age classification used in this study at 17 dpi. 14 Figure 3-1 Effects of two phototrophic bacterial inoculants (R. palustris PS3 and C3) on the growth of Chinese cabbage. 26 Figure 3-2 Absolute growth rate (A) and relative growth rate (B) of Chinese cabbage under different treatments. 28 Figure 3-3 Root morphologies of Chinese cabbage in response to bacterial treatments at 8 DAP. 29 Figure 3-4 Nitrogen contents in the shoots of Chinese cabbage. 31 Figure 3-5 Real-time PCR analyses of gene expressions of nitrate transporter in roots in response to R. palustris PS3 and C3 inoculation at 8,11,17 DAP. (A) BjNRT1.1, (B) BjNRT1.2, (C) BjNRT2.1. T 34 Figure 3-6 Nitrate contents in leaf tissues of Chinese cabbage inoculated with R. palustris PS3 (50%NS+P3) or C3 (50%NS+C3). 35 Figure 3-7 Nitrogen assimilation associated enzyme activities of Chinese cabbage in the shoots. 37 Figure 3-8 Soluble protein contents of Chinese cabbage in the shoots. 39 Figure 3-9 Nitrogen use efficiency (NUE), nitrogen uptake efficiency (NupE), nitrogen utilization efficiency (NutE) of Chinese cabbage at 11 DAP and 17 DAP. 40 Figure 3-10 Total chlorophyll contents in the leaves of Chinese cabbage at 8 DAP (A) and 17 DAP (B). 42 Figure 3-11 Shoot carbon content in of Chinese cabbage inoculated R.palustris PS3 (50%NS+P3) and R.palustris C3 (50%NS+C3) or non-inoculated (50%NS). 43 Figure 3-12 Effects of R. palustris PS3 on photosynthetic rate (A), stomatal conductance (B), transpiration (C), and intercellular CO2 concentration (D) of Chinese cabbage. 45 Figure 3-13 Estimated RuBisCO contents in leaves of Chinese cabbage. 48 Figure 3-14 C:N ratio of Chinese cabbage at 17 DAP. C:N ratio was calculated with dry weight. 50 表目錄 Table 2-1 Primers used in this study. 23 Table 3-1 Contents of nitrate in hydroponic solutions at 0 and 17 DAP. 32 | |
| dc.language.iso | en | |
| dc.title | Rhodopseudomonas palustris PS3光合菌對於小白菜氮與碳代謝之影響 | zh_TW |
| dc.title | Effects of Rhodopseudomonas palustris PS3 on nitrogen and carbon metabolism in the Brassica rapa L. ssp. chinensis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 李昆達(Kung-Ta Lee) | |
| dc.contributor.oralexamcommittee | 劉?睿,陳仁治,楊健志 | |
| dc.subject.keyword | 促進植物生長根圈微生物,沼澤紅假單胞菌,氮素吸收效率,碳素同化,硝酸鹽轉運蛋白, | zh_TW |
| dc.subject.keyword | Plant growth promoting rhizobacteria (PGPR),Rhodopseudomonas palustris,nitrogen uptake efficiency,C assimilation,nitrate transporter, | en |
| dc.relation.page | 72 | |
| dc.identifier.doi | 10.6342/NTU201602060 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2016-08-15 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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