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標題: | 茉莉酸參與印度梨形孢真菌增強水稻瘤野螟耐受性的研究 Involvement of jasmonates in the Piriformospora indica – enhanced tolerance of rice to leaffolder (Cnaphalocrocis medinalis) |
作者: | 陳芝云 Chih-Yun Chen |
指導教授: | 王淑珍 Shu-Jen Wang |
關鍵字: | 水稻,印度梨形孢真菌,瘤野螟,茉莉酸,胰蛋白?抑制劑, rice,Piriformospora indica,rice leaffolder,jasmonate,trypsin inhibitor, |
出版年 : | 2019 |
學位: | 碩士 |
摘要: | 印度梨型孢菌(Piriformospora indica, P. indica)為可促進植物根系發育及增進生長勢之根部共生真菌。以水稻品種臺中在來一號 (Oryza sativa L. cv. Taichung Native 1, TCN1) 為材料,我們研究室先前的實驗結果顯示經P. indica共生後,水稻受瘤野螟幼蟲侵咬所造成的危害程度明顯下降,且部分茉莉酸 (jasmonic acid, JA) 生合成酵素基因之表現及胰蛋白酶抑制劑 (trypsin inhibitor, TI) 活性較未經P. indica共生的植株高。本研究主要探討JA是否為P. indica增強水稻TI活性之重要訊息因子。LC-MS分析得知P. indica共生增強瘤野螟幼蟲侵食誘導JA生合成之效應,並以水稻osjar1突變體進行試驗,結果發現降低JA生合成將會減低TI活性,且原本P. indica共生後所提升之抗蟲優勢減弱; 再外加乙醯水楊酸以抑制JA生合成,結果顯示在P. indica共生的植株,原本受瘤野螟刺激而TI活性增加的現象則不顯著。故以上兩試驗之結果均顯示JA訊息參與P. indica誘導TI活性上升之調控機制。另外,P. indica共生亦會調控受瘤野螟幼蟲侵食植株中類黃酮含量與抗氧化酵素活性。在植株生長勢部分P. indica促使水稻葉鞘變寬且鮮重增加,並促進木質素累積,推測其可增強水稻物理性防禦機制。綜合以上結果,P. indica可透由提升抗氧化能力、增加抗蟲相關二次代謝物表現,並經由JA途徑調控TI活性,以提升抗瘤野螟幼蟲侵食之效能。 Piriformospora indica (P. indica) is a symbiotic root endophyte that promotes plant root development and increases growth potential. The research in our lab focused on studying the effect of P. indica on rice (Oryza sativa L. cv. Taichung native 1, TCN1) tolerance against rice leaffolder (Cnaphalocrocis medinalis Guenée) and the related P. indica induced herbivore defense mechanisms. Previous data showed the leaf injury conducted by leaffolder larvae in P. indica-colonized rice was lower than that in non-colonized plants. Several jasmonate-related gene expressions and trypsin inhibitor (TI) activities in leaf tissues of P. indica-colonized plants were higher than those were in non-colonized plants under leaffolder-infestated conditions. Therefore, it was suggested that P. indica enhance rice leaffolder tolerance of rice plants may be mediated JA-related signaling pathway. In this study, rice leaffolder-activated TI activity in P. indica-inoculated osjar1 mutant plants was decreased. Moreover, the enhance of TI activity in rice plants treated with aspirin, an inhibitor of jasmonate synthesis, was also declined. Taken together, P. indica enhances TI activities of rice plants was mediated by JA signaling pathway. Furthermore, antioxidant mechanisms were involved in P. indica-enhanced insect tolerance of rice. The amount of defense-related metabolites of P. indica - colonized seedlings were also increased. In summary, P. indica activated antioxidant mechanisms, defensive metabolite accumulation and JA-induced TI activity to increase herbivory insect tolerance of rice seedlings. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78813 |
DOI: | 10.6342/NTU201900564 |
全文授權: | 未授權 |
電子全文公開日期: | 2024-02-15 |
顯示於系所單位: | 農藝學系 |
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