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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林雅芬 | zh_TW |
| dc.contributor.author | 陳郁綾 | zh_TW |
| dc.contributor.author | Yu-Ling Chen | en |
| dc.date.accessioned | 2021-07-11T15:02:37Z | - |
| dc.date.available | 2024-08-20 | - |
| dc.date.copyright | 2019-08-28 | - |
| dc.date.issued | 2019 | - |
| dc.date.submitted | 2002-01-01 | - |
| dc.identifier.citation | Andreini, C., Banci, L., Bertini, I., and Rosato, A. (2006). Zinc through the three domains of life. Journal of Proteome Research, 5(11), 3173-3178.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78536 | - |
| dc.description.abstract | 膳食中的鋅攝取不足已經成為在全世界廣泛發生的問題。瞭解植物尤其是水稻如何調控鋅元素的吸收運輸將有助於我們提高作物中的鋅含量。阿拉伯芥轉錄因子bZIPs (the basic Leucine Zipper Proteins)，AtbZIP19及AtbZIP23透過與 AtZIP4 基因啟動子區域 的Zinc Deficiency Response Element (ZDRE) 之結合，來調控 AtZIP4 轉運子基因的表達，進而調控阿拉伯芥鋅的吸收和運輸；然而，其水稻同源基因(F-OsbZIPs：OsbZIP07, 44, 53) 對鋅元素調控機制目前仍不清楚。為瞭解F-OsbZIPs是否參與水稻鋅之調控機制，本研究以水稻(Oryza sativa L. cv. kitaake) 為材料，以水耕試驗進行不同鋅濃度的處理 (0.002, 0.2, 300 µM)，並分析生理性狀以及基因表現。實驗結果顯示處理後第21天，不論是缺鋅(0.002μM)或過量鋅(300μM)處理，其株高、根長、葉綠素含量、鮮重、乾重等相關生理數值均顯著低於對照組(0.2μM)。基因表現分析結果發現水稻F-OsbZIPs基因幾乎不受鋅處理改變其表現，而具ZDRE基因(OsZIP5, OsZIP7, OsZIP9 )在處理後第三天開始受缺鋅誘導表現，過量鋅則會抑制其表現。而為了對水稻在不同鋅處理下的轉錄體變化有全面的了解，我們同時也進行RNA sequencing (RNA-seq)，發現在216個具ZDRE基因之中，共有20個為差異性表現基因，且表現出受缺鋅誘導及過量鋅抑制的表現模式，結果暗示這些具ZDRE基因可能參與水稻的鋅調控之機制。綜上所述，水稻的F-OsbZIPs轉錄因子可能具有與AtbZIP19、AtbZIP23相似的鋅調控機制，這項發現對於瞭解水稻調控鋅元素吸收運輸的機制以及未來進行水稻的鋅生物營養強化十分重要。 | zh_TW |
| dc.description.abstract | Insufficient Zinc (Zn) intake in the diet has become a series problem, especially for children. To increase the concentration of Zn in crops e.g. rice, it is necessary to understand the Zn‐homeostatic mechanisms in plants. It is still not clear how bZIP transcription factors (F-OsbZIPs: OsbZIP07, 44, 53) are involve in the regulatory mechanism of Zn in rice. To verify the role of F-OsbZIPs and their downstream genes in Zn homeostsis in rice (Oryza Sativa L. Kitaake), this study compared the differential physiologicl and molecular responses undedr different Zn concentration (0.002, 0.2, 300μM). The physiologicl results showed that plant height, root length, fresh weight and dry weight, chlorophyll contents in Zn deficient (0.002) and excess Zn (300μM) treatments were significantly lower than in the control condition (0.2 μM). The molecular analysis indicated that F-OsbZIPs were expressed constitutively while their downstream candidate genes, twenty ZDRE-containing genes (inclusive of OsZIP5, OsZIP7, OsZIP9), are induced under zinc deficiency, but repressed by excess Zn. Overall speaking, our findings imply that the rice F-OsbZIPs exist a similar Zn regulatory mechanism to Arabidopsis AtbZIP transcription factors AtbZIP19 and AtbZIP23. This finding is important to understand the mechanisms of Zn uptake and transport for future biofortification pupose in rice. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-11T15:02:37Z (GMT). No. of bitstreams: 1 ntu-108-R06621105-1.pdf: 12751493 bytes, checksum: 616bb6bc63c0267ce57b57cf4b934c33 (MD5) Previous issue date: 2019 | en |
| dc.description.tableofcontents | 口試委員審定書 i
誌謝 ii 中文摘要 iii 英文摘要 iv 目錄 v 圖目錄 ix 表目錄 xi 附圖及附表目錄 xii 縮寫表 1 前言 2 1. 鋅元素的重要性 2 2. 鋅元素吸收及運輸之分子機制 4 3. 鋅元素調控機制之研究 5 4. 本論文研究目的 6 材料方法 8 1. 實驗材料種植與鋅處理 8 1.1 植物材料與品種 8 1.2 水稻種子消毒與催芽 8 1.3 水耕系統設置 8 1.4 水耕液配方 9 1.5 人工照明室環境設置 9 1.6 鋅濃度試驗處理 9 1.7 樣品之均質化 9 2. 葉綠素含量測定 10 2.1 以葉綠素計(SPAD) 測量相對葉綠素含量 10 2.2 萃取葉片之葉綠素含量測定 10 3. ROS (reactive oxygen species)染色分析 11 3.1 DAB (3, 3 -diaminobenzidine)染色法 11 3.2 NBT (Nitro Blue Tetrazolium)染色法 11 4. 抗氧化酵素活性分析 12 4.1 樣品準備與萃取 12 4.2 蛋白質定量 12 4.3 Ascorbate peroxidase (APX) 活性測定 12 4.4 Catalase (CAT)活性測定 13 4.5 Glutathione reductase (GR) 13 5. OsbZIPs與AtbZIP19、AtbZIP23親緣關係分析 14 5.1 AtbZIP19、AtbZIP23以及OsbZIPs序列的獲取 14 5.2 以MEGA進行親緣關係分析 14 6. 水稻F-OsbZIPs序列之獲取 14 6.1 從網站上取得水稻F-bZIPs序列 14 6.2 驗證水稻F-bZIPs序列 15 7. F-OsbZIPs序列比對及motif分析 20 7.1 序列比對、保守性分析以及二級結構預測 20 7.2 Motif分析 21 8. 水稻ZDRE基因分析 21 8.1 水稻全基因組ZDRE之分析 21 8.2 ZDRE基因 之Gene Ontology (GO)分析 22 9. RNA萃取與cDNA合成 22 10. RNA定序 (RNA Sequencing，RNA-seq) 22 11. Real-time PCR (qPCR) 24 12. 統計分析 25 結果 26 1. 水稻對鋅處理之生理反應 26 2. 水稻對鋅元素之分子調控 28 3. OsbZIPs與AtbZIP19, 23之親緣關係 30 4. 水稻F-OsbZIPs之序列 30 4.1 數據庫F-OsbZIPs序列 31 4.2 F-OsbZIPs基因序列 32 4.3 F-OsbZIPs蛋白質序列分析 32 5. 水稻啟動子ZDRE分析 33 6. 水稻鋅元素調控相關基因之表現量 34 討論 36 1. 水稻幼苗缺鋅及過量鋅之生理反應 36 2. 水稻幼苗調控鋅元素之分子機制 38 3. 水稻F-OsbZIPs序列之品種差異性 41 4. F-OsbZIPs蛋白質序列具有保守性 42 5. F-OsbZIPs調控水稻ZDRE基因 43 6. F-OsbZIPs 及ZDRE基因之表現量 44 7. 結論與未來展望 45 圖 46 表 82 附錄圖 88 附錄表 121 參考文獻 133 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | bZIP轉錄因子 | zh_TW |
| dc.subject | 生物營養強化 | zh_TW |
| dc.subject | 鋅 | zh_TW |
| dc.subject | ZIP轉運蛋白 | zh_TW |
| dc.subject | 水稻 | zh_TW |
| dc.subject | bZIPs | en |
| dc.subject | biofortification | en |
| dc.subject | zinc | en |
| dc.subject | ZIP transporter | en |
| dc.subject | rice (Oryza sativa) | en |
| dc.title | 鋅元素對水稻生理及分子機制調控之研究 | zh_TW |
| dc.title | Physiological and Molecular Regulation of Rice in Response to Zn | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 107-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 楊淑怡;陳賢明;蔡皇龍 | zh_TW |
| dc.contributor.oralexamcommittee | ;; | en |
| dc.subject.keyword | 水稻,bZIP轉錄因子,ZIP轉運蛋白,鋅,生物營養強化, | zh_TW |
| dc.subject.keyword | rice (Oryza sativa),bZIPs,ZIP transporter,zinc,biofortification, | en |
| dc.relation.page | 139 | - |
| dc.identifier.doi | 10.6342/NTU201903384 | - |
| dc.rights.note | 未授權 | - |
| dc.date.accepted | 2019-08-18 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 農藝學系 | - |
| dc.date.embargo-lift | 2024-08-28 | - |
| 顯示於系所單位： | 農藝學系 | |
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