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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 楊台鴻(Tai-Horng Young) | |
| dc.contributor.author | Shen Yang | en |
| dc.contributor.author | 楊森 | zh_TW |
| dc.date.accessioned | 2021-07-11T14:45:40Z | - |
| dc.date.available | 2026-12-31 | |
| dc.date.copyright | 2016-10-14 | |
| dc.date.issued | 2016 | |
| dc.date.submitted | 2016-07-25 | |
| dc.identifier.citation | [1] Johl SS, Burgett RA. Dermal filler agents: a practical review. Current opinion in ophthalmology. 2006;17:471-9.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78199 | - |
| dc.description.abstract | 注射式皮膚填充物是近年來常被應用在醫學美容的生醫材料，藉由具生物相容性與生物降解性生醫材料來製備,而膠原蛋白為其中一種常見的填充物材料，膠原蛋白具有良好生物相容性、生物可降解性且具有許多蛋白質鍵結區域可供細胞貼附近而促進細胞增生，而聚乳酸聚乙醇酸共聚物為一種生物可降解性高分子，藉由乳化法可以製作藥物載體並且提供持續釋放的效果。
抗壞血酸,又通稱維他命C，為一種水溶性的抗氧化劑，其參與人體許多的生理反應，如膠原蛋白生成、細胞增生、氧化還原反應、與免疫系統的反應……等等。因在本研究中，我們利用乳化法揮發法將抗壞血酸包覆於聚乳酸聚乙醇酸共聚物奈米粒子中，並包覆於膠原蛋白水膠中，製造一種可以持續釋放抗壞血酸約14天之皮下填充物，藉由聚乳酸聚乙醇酸共聚物微米粒子保護易氧化的抗壞血酸，而這樣的材料在細胞實驗中確實可以增加纖維細胞膠原蛋白的生成量與細胞活性 ，而在天竺鼠的動物實驗中也可以確實增加真皮層的厚度,相較於控制組約有百分之七的增加量，希望藉由此材料提供填充物一個更有效的生醫材料。 | zh_TW |
| dc.description.abstract | Injectable filler is widely used in facial and bone rejuvenation, in this research, we combining the PLGA drug delivery system, l-ascorbic acid and collagen hydrogel into injectable filler. The PLGA polymers were widely in drug delivery system. Because of its remarkable biocompatibility and biodegradability. The collagen hydrogel can extend the drug releasing time and widely used in dermatology which has abilities of biocompatible, degradable and its great cellular interactions. The l-ascorbic acid can enhance cell viability and increase the secretion of collagen by fibroblast.
We proposed that when the filler degrade slowly the ascorbic acid also released and the drug will stimulate the fibroblast to proliferation and secreting the collagen. And the secreted collagen and cell proliferation can enhance the facial rejuvenation ability of the filler. This research showed that the filler combining to l-ascorbic acid could successfully increase the cell viability and secretion of collagen by fibroblast. We hope this material could provide a better choice for cosmetic surgery. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-11T14:45:40Z (GMT). No. of bitstreams: 1 ntu-105-R03548012-1.pdf: 2737554 bytes, checksum: 50cd47e2dbbd0d5a0f4dc7e52d9cc199 (MD5) Previous issue date: 2016 | en |
| dc.description.tableofcontents | 致謝 i
中文摘要 ii Abstract iii Content iv List of Tables vii List of Figures viii Chapter 1 Introduction 1 1.1 Dermal Filler 1 1.2 Fibroblast 2 1.3 Collagen 3 1.4 PLGA 4 1.5 L-Ascorbic acid 5 1.6 The Aim 6 Chapter 2 Materials and Methods 7 2.1 Biological and Chemical Reagents 7 2.2 Instruments 8 2.3 Culture Medium Preparation 9 2.4 Collagen Solution preparation 9 2.5 Cell Culture 9 2.6 L-Ascorbic acid Loaded PLGA Particle Preparation 10 2.7 In Vitro Drug Release 10 2.8 Cell Viability Assay 10 2.9 Collagen Assay 11 2.10 Western blot 12 2.11 Animal Experiment 12 Chapter 3 Results 14 3.1 Particles Structure Analysis 14 3.2 Drug Release Profile 15 3.3 Cell Morphologies and Cell Viability After Treatment 16 3.4 Collagen Assay 17 3.5 Histology 19 Chapter 4 Discussion 20 Chapter 5 Conclusion and Perspective 23 Figures 24 Tables 39 References 40 | |
| dc.language.iso | en | |
| dc.subject | 聚乳酸聚乙醇酸共聚物 | zh_TW |
| dc.subject | 皮膚填充物 | zh_TW |
| dc.subject | 抗壞血酸 | zh_TW |
| dc.subject | 膠原蛋白 | zh_TW |
| dc.subject | l-ascorbic acid | en |
| dc.subject | injection filler | en |
| dc.subject | collagen | en |
| dc.subject | PLGA | en |
| dc.title | 膠原蛋白水膠包覆含抗壞血酸之聚乳酸聚乙醇酸共聚物粒子之應用 | zh_TW |
| dc.title | Application of collagen hydrogel containing l-ascorbic acid loaded PLGA particles | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 104-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林頌然(Sung-Jan Lin),王大銘(Da-Ming Wang),賴君義(Juin-Yih Lai) | |
| dc.subject.keyword | 皮膚填充物,膠原蛋白,聚乳酸聚乙醇酸共聚物,抗壞血酸, | zh_TW |
| dc.subject.keyword | injection filler,collagen,PLGA,l-ascorbic acid, | en |
| dc.relation.page | 46 | |
| dc.identifier.doi | 10.6342/NTU201601035 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2016-07-25 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| dc.date.embargo-lift | 2026-12-31 | - |
| 顯示於系所單位： | 醫學工程學研究所 | |
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