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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 陳力騏 | |
dc.contributor.author | Guan-Cheng Lin | en |
dc.contributor.author | 林冠成 | zh_TW |
dc.date.accessioned | 2021-07-11T14:43:52Z | - |
dc.date.available | 2021-10-26 | |
dc.date.copyright | 2016-10-26 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-08-08 | |
dc.identifier.citation | 1. 財政部關務署。2016。統計資料庫查詢系統。台北:財政部關務署。網址:https://portal.sw.nat.gov.tw/APGA/GA01。上網日期:2016-05-20。
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Development of a capillary electrophoresis method for the simultaneous analysis of artificial sweeteners, preservatives and colours in soft drinks. Journal of Chromatography A 876(1): 213-220. 21. Gautam, D., Sinha, R. K., & Milne, D. B. 1986. Interaction of Ponceau 4R with Cooper and Effect of Feeding Ponceau 4R on Iron Metabolism. Journal of Food Science and Technology 23(6): 303-307. 22. Granby, K., & Vahl, M. 2001. Investigation of the herbicide glyphosate and the plant growth regulators chlormequat and mepiquat in cereals produced in Denmark. Food Additives & Contaminants 18(10): 898-905. 23. Granby, K., Johannesen, S., & Vahl, M. 2003. Analysis of glyphosate residues in cereals using liquid chromatography-mass spectrometry (LC-MS/MS). Food Additives & Contaminants 20(8): 692-698. 24. Herrmann, K. M., & Weaver, L. M. 1999. The shikimate pathway. Annual review of plant biology 50(1): 473-503. 25. Institute of Science in Society. 2014. Widespread Glyphosate Contamination in USA. 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Stability-Constants of Copper(II), Zinc, Manganese(II), Calcium, and Magnesium Complexes of N-(Phosphonomethyl)Glycine (Glyphosate). Acta Chem Scand 32: 79-83. 31. Minioti, K. S., Sakellariou, C. F., & Thomaidis, N. S. 2007. Determination of 13 synthetic food colorants in water-soluble foods by reversed-phase high-performance liquid chromatography coupled with diode-array detector. Analytica Chimica Acta 583(1): 103-110. 32. Moco, S., Martin, F. P. J., & Rezzi, S. 2012. Metabolomics view on gut microbiome modulation by polyphenol-rich foods. Journal of proteome research 11(10): 4781-4790. 33. Moorman, T. B., Becerril, J. M., Lydon, J., & Duke, S. O. 1992. Production of hydroxybenzoic acids by Bradyrhizobium japonicum strains after treatment with glyphosate. Journal of Agricultural and Food Chemistry 40(2): 289-293. 34. Nafziger, E. D., Widholm, J. M., Steinrücken, H. C., & Killmer, J. L. 1984. Selection and characterization of a carrot cell line tolerant to glyphosate. Plant physiology 76(3): 571-574. 35. Paetow, L. J., McLaughlin, J. D., Pauli, B. D., & Marcogliese, D. J. 2013. Mortality of American bullfrog tadpoles lithobates catesbeianus infected by Gyrodactylus jennyae and experimentally exposed to Batrachochytrium dendrobatidis. Journal of aquatic animal health 25(1): 15-26. 36. Pilu, R. 2013. Long term toxicity of a Roundup herbicide and a Roundup-tolerant genetically modified maize. Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 53: 454. 37. Pintado, S., Montoya, M. R., Rodriguez-Amaro, R., Mayen, M., & Mellado, J. M. R. 2012. Electrochemical Determination of Glyphosate in Waters Using Electrogenerated Copper Ions. Int. J. Electrochem. Sci 7: 2523 – 2530. 38. Piriyapittaya, M., Jayanta, S., Mitra, S., & Leepipatpiboon, N. 2008. 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Current Microbiology 66(4):350-358. 47. SIGMA-ALDRICH. 2016. China (Mainland)- 18137 - Ponceau 4R, Available at: http://www.sigmaaldrich.com/catalog/product/sial/18137?lang=zh®ion=CN. 48. Tanaka, Y., & Terabe, S. 2002. Estimation of binding constants by capillary electrophoresis. Journal of Chromatography B 768(1): 81-92. 49. Thompson, M., Ellison, S. L., & Wood, R. 2006. The international harmonized protocol for the proficiency testing of analytical chemistry laboratories (IUPAC Technical Report). Pure and Applied Chemistry 78(1): 145-196. 50. Turak, F., Dinç, M., Dülger, Ö., & Özgür, M. U. 2015. Four Derivative Spectrophotometric Methods for the Simultaneous Determination of Carmoisine and Ponceau 4R in Drinks and Comparison with High Performance Liquid Chromatography. Hindawi Publishing Corporation International Journal of Analytical Chemistry 2014: 1-11. 51. Undabeytia, T., Morillo, E., & Maqueda, C. 2002. FTIR Study of Glyphosate-Copper Complexes. Journal of Agricultural and Food Chemistry 50(7): 1918-1921. 52. Van Nuijs, A. L., Tarcomnicu, I., & Covaci, A. 2011. Application of hydrophilic interaction chromatography for the analysis of polar contaminants in food and environmental samples. Journal of Chromatography A 1218(35): 5964-5974. 53. Walorczyk, S., & Drożdżyński, D. 2012. Improvement and extension to new analytes of a multi-residue method for the determination of pesticides in cereals and dry animal feed using gas chromatography–tandem quadrupole mass spectrometry revisited. Journal of Chromatography A 1251: 219-231. 54. Wang, Z. X., Kumar, N. R., & Srivastava, D. K. 1992. A Novel Spectroscopic Titration Method for Determining the Dissociation Constant and Stoichiometry of Protein-Ligand Complex. Analytical Biochemistry 206(2): 376-381. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78150 | - |
dc.description.abstract | 胭脂紅是一種亮紅色的染色劑,其鮮豔的顏色容易在與銅離子作用後消退。雖然過去有幾次關於胭脂紅和銅離子交互作用的研究,但是學者們對其作用機制與化學計量關係,卻沒有給出一致性的答案。本研究利用吸光係數與結合常數的關係,全面性地研究在不同pH值下銅離子-胭脂紅錯合物的化學計量關係與結合常數。結果顯示染劑和金屬離子的化學計量關係受pH值影響,在pH 4.0—7.0間,為一分子銅離子鍵結一分子胭脂紅;在pH 8.0—10.0間,為三分子銅離子鍵結四分子胭脂紅;而在pH 11.0時,則為一分子銅離子鍵結三分子胭脂紅。此外,全世界最暢銷的除草劑-嘉磷塞,其本身也與銅離子有著極易螯合的特性。由於嘉磷塞和胭脂紅在酸性環境下,兩者分別與銅離子形成錯合物後,其穩定性有著極大差異 (在pH 5.0下,銅離子-胭脂紅錯合物的穩定結合常數為1.37 × 104,而嘉磷塞-銅離子錯合物的穩定結合常數則為1.26 × 1018),故利用嘉磷塞和胭脂紅會競爭銅離子的行為,可以設計出一款銅離子-胭脂紅試劑 (20µM銅離子-40µM胭脂紅),在嘉磷塞加入試劑後,解離的銅離子-胭脂紅錯合物會造成明顯的變色行為,在吸收光譜法下 (λ = 506 nm) 觀察此變色現象就可以準確定量嘉磷塞含量,線性檢測濃度範圍為5—20 µM (CV < 5%, n = 3),檢測極限為1 µM。 | zh_TW |
dc.description.abstract | Ponceau 4R is a brilliant red dye which is easily bleached due to the chelation with copper ion. Although the interaction between ponceau 4R and copper ion has been investigated extensively, there is no consensus about the detailed mechanism and the stoichiometry. The stoichiometries and binding constants of copper-ponceau 4R complex were studied under different pH values by calculating the relationship between extinction coefficient and binding constant. The results showed that the formation ratio of metal:dye was dependent on the pH value in the system. Three different pH range were tested, which were pH 4.0—7.0, pH 8.0—10.0 and pH 11.0, the metal:dye ratio formed were 1:1, 3:4 and 1:3 respectively. Furthermore, the world's best-selling herbicide, glyphosate, is also found to be very easy to chelate copper ion. Due to the great difference in the stabilities of copper-ponceau 4R complex and copper-glyphosate complex under acidic condition (binding constant of copper-ponceau 4R complex is 1.37×104 at pH 5.0 and copper-glyphosate complex’s is 1.26×1018 at pH 5.0), hence, a copper-ponceau 4R reagent (20µM copper ion-40µM ponceau 4R) was designed based on the competitive reaction between ponceau 4R and glyphosate in this study. After adding glyphosate to the solution, an obvious color change was occurred due to the dissociation of copper-ponceau 4R complex and the change in absorbance can be detected with the presence of glyphosate precisely (λ = 506 nm). The linear dynamic range for glyphosate was 5—20 µM (CV < 5%, n = 3) and the limit of detection (LOD) was 1 µM. | en |
dc.description.provenance | Made available in DSpace on 2021-07-11T14:43:52Z (GMT). No. of bitstreams: 1 ntu-105-R03631049-1.pdf: 2883884 bytes, checksum: f5894cf2f3687903f9121de43feccae6 (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | 口試委員會審訂書 i
誌謝 ii 中文摘要 iii Abstract iv 目錄 v 圖目錄 vii 表目錄 viii 第一章 前言 1 第二章 文獻探討 2 2.1胭脂紅與銅離子之交互作用 2 2.1.1胭脂紅簡介 2 2.1.2銅離子造成胭脂紅的褪色現象 4 2.1.3胭脂紅與銅離子鍵結的化學計量關係 7 2.1.4胭脂紅的螢光特性 9 2.2嘉磷塞 10 2.2.1嘉磷塞簡介 10 2.2.2基改食品與嘉磷塞的關係 12 2.2.3嘉磷塞對健康與環境造成的隱憂 14 2.2.4現行檢測嘉磷塞的方法 17 第三章 材料與方法 19 3.1化學藥品 19 3.2試劑與緩衝溶液配製 20 3.2.1試劑配製 20 3.2.2緩衝溶液配製 21 3.3實驗儀器設備 22 3.4研究胭脂紅與銅離子交互作用之方法建立 23 3.4.1於吸收光譜法下研究胭脂紅與銅離子之交互作用 23 3.4.2於螢光法下研究胭脂紅與銅離子之交互作用 24 3.5研究胭脂紅、銅離子、嘉磷塞競爭反應之方法建立 25 3.6檢測嘉磷塞之方法建立 26 3.7干擾物測試 27 3.8從黃豆中萃取嘉磷塞之方法 28 第四章 結果與討論 29 4.1胭脂紅和銅離子之交互作用 29 4.1.1胭脂紅褪色之吸收光譜變化 29 4.1.2判定胭脂紅與銅離子反應的化學計量關係之方法選擇 31 4.1.3 pH值對胭脂紅與銅離子反應的化學計量關係與結合常數之影響 34 4.1.4研究胭脂紅與銅離子反應之螢光特性 37 4.2胭脂紅、銅離子、嘉磷塞之交互作用 41 4.2.1胭脂紅與嘉磷塞競爭銅離子之溶液pH值選擇 41 4.2.2胭脂紅與嘉磷塞競爭銅離子之強弱判定 43 4.3檢測嘉磷塞 45 4.3.1利用嘉磷塞造成銅離子-胭脂紅溶液吸光值變化的特性定量嘉磷塞 45 4.3.2試劑中的胭脂紅與銅離子之最佳化濃度探討 46 4.3.3干擾物測試 47 4.3.4檢測黃豆製品之嘉磷塞殘留 49 第五章 結論與未來展望 51 參考文獻 52 | |
dc.language.iso | zh-TW | |
dc.title | 胭脂紅與銅離子錯合之研究並應用於檢測外源性化合物質 | zh_TW |
dc.title | Study on the Complexation of Ponceau 4R with
Copper and Its Application on Xenobiotics Detection | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 謝博全,周呈霙,陳珮珊 | |
dc.subject.keyword | 胭脂紅,銅離子,螯合,化學計量學,競爭型反應,外源性化合物質, | zh_TW |
dc.subject.keyword | Ponceau 4R,Copper ion,Chelation,Stoichiometry,Competitive reaction,Xenobiotics, | en |
dc.relation.page | 59 | |
dc.identifier.doi | 10.6342/NTU201601661 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2016-08-08 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 生物產業機電工程學研究所 | zh_TW |
顯示於系所單位: | 生物機電工程學系 |
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