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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 董成淵(Chen-Yuan Dong) | |
dc.contributor.author | Ya-Lin Huang | en |
dc.contributor.author | 黃雅琳 | zh_TW |
dc.date.accessioned | 2021-07-11T14:43:46Z | - |
dc.date.available | 2021-10-14 | |
dc.date.copyright | 2016-10-14 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-08-09 | |
dc.identifier.citation | [1] Copyright © 2016 Healthhype.com
[2] Chu, Y.-W., P.-C. Yang, S.-C. Yang, Y.-C. Shyu, M. J. C. Hendrix, R. Wu, and C.-W. Wu. 1997. Selection of invasive and metastatic subpopulations from a human lung adenocarcinoma cell line. Am. J. Respir. Cell Mol. Biol. 17:353–360. [3] 2008 美國癌症協會(American Cancer Society)『非小細胞腺癌概述』 [4] Pocket companion to Robbins pathologic basis of disease [5] Denk, W. Strickler, JH. Webb, WW. Two-photon laser scanning fluorescence microscopy. Science, 1990.248:p73-76. [6] http://www.olympusmicro.com/primer/java/jablonski/jabintro/ [7] The Molecular Probes® Handbook A Guide to Fluorescent Probes and Labeling Technologies11th Edition (2010) [8] Manjubharathi, S. and S. Saraswathi 'CANCER CELL SEGMENTATION AND DETECTION USING NC RATIO ANALYSIS.' [9] Schmitz, C., et al. (2014). 'Current automated 3D cell detection methods are not a suitable replacement for manual stereologic cell counting.' Frontiers in Neuroanatomy [10] Gryczynski, I., et al. (1996). 'Multiphoton excitation of the DNA stains DAPI and Hoechst.' Bioimaging 4(3): 138-148. [11] Zhang, M. L., et al. (2016). 'Morphologists overestimate the nuclear-to-cytoplasmic ratio.' Cancer Cytopathology. [12] Thiran, J.-P. and B. Macq (1996). 'Morphological feature extraction for the classification of digital images of cancerous tissues.' IEEE Transactions on biomedical engineering 43(10): 1011-1020. [13] Lung Cancer: A Multidisciplinary Approach to Diagnosis and Management [14] The Molecular Probes® Handbook A Guide to Fluorescent Probes and Labeling Technologies11th Edition (2010) [15] Abràmoff, M. D., et al. (2004). 'Image processing with ImageJ.' Biophotonics international 11(7): 36-42. [16] Copyright © 2011 National Diagnostics. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78147 | - |
dc.description.abstract | 肺腺癌屬於非小細胞癌,主要的來源是肺部外層的表皮細胞變異,因為早期癌變的狀態並非塊狀或腫瘤,亦無症狀,x-ray檢測發現的時機通常已是癌末。將癌末細胞從人體取出體外複製培養成具有穩定繼代特性細胞株(cell line),再依據其轉移能力與惡化程度分成CL1-0與CL1-5。根據病理學,癌細胞的細胞核大小與細胞成熟度有關聯,在本研究中,我們利用螢光染劑的特性與雙光子螢光顯微鏡拍攝平面與不同深度的影像。藉由ImageJ分析這些影像資料,圈選細胞核與細胞輪廓得到面積的比例關係。發現CL1-5的細胞核面積較CL1-0大,NC比例則是CL1-0的大於CL1-5。 | zh_TW |
dc.description.abstract | Adenocarcinoma cancer, a type of non-small cell lung cancer, is caused by the variation of the epithelial cells in the outer layer of lung. Because in the early state, the adenocarcinoma cancer does not form blocks nor cancerous tumor, may also be asymptomatic, the x-ray detectable cancer is usually in terminal state. The cancer cells derived from patients were replicated in vitro and cultured into cell lines. According to their invasive ability and degree of deterioration, the cell lines were divided into CL1-0 and CL1-5. Based on pathology, the cell nuclei size is associated with the maturity of the cancer cells. In this study, we used fluorescent dye properties and two-photon fluorescence microscopy to image these two types of cells at different depths. By using ImageJ, we analyzed the image data, circling the nucleus and cell outline to obtain the proportion of nucleus to cytoplasm. In my research the nuclei area of CL1-5 is bigger than CL1-0; the nuclear-to-cytoplasmic ratio (NC ratio) of CL1-0 is greater than CL1-5. | en |
dc.description.provenance | Made available in DSpace on 2021-07-11T14:43:46Z (GMT). No. of bitstreams: 1 ntu-105-R03222080-1.pdf: 7988220 bytes, checksum: 3ffe2eeb829b0ee8bb748b945af41dbc (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | 目錄
誌謝 II 中文摘要 V ABSTRACT VI 目錄 VII 圖目錄 IX CHAPTER1 緒論 1 1.1研究動機 1 1.2 惡性腫瘤簡介 1 1.3 細胞的核質比 4 CHAPTER2 基本原理 6 2.1顯微術 6 2.1.1 顯微鏡原理 7 2.1.2 光學解析度 8 2.2 單光子的螢光原理 10 2.3 雙光子的螢光原理 14 2.3其他螢光性質 15 2.4 與共軛焦顯微鏡的比較 16 CHAPTER3 實驗架設 18 3.1雷射光源的非線性光學顯微鏡 18 3.2光學系統架設 19 3.3螢光染劑介紹 20 CHAPTER4 實驗材料與方法 24 4.1 肺腺癌細胞的篩選 24 4.2 細胞培養 26 4.3 細胞染色程序 26 4.4 實驗材料介紹 27 4.5 實驗分析方法 28 CHAPTER5 實驗結果 32 CHAPTER6 問題討論與未來展望 38 6.1 實驗問題討論 38 6.2 未來展望 39 參考資料 41 | |
dc.language.iso | zh-TW | |
dc.title | 利用雙光子螢光顯微鏡之影像分析肺腺癌細胞核質比 | zh_TW |
dc.title | Survey on Lung Adenocarcinoma Cells Nuclear-to-Cytoplasmic Ratio Analysis for Two-photon Fluorescence Microscopy Images | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳惠文(Huei-Wen Chen),張顏暉(Yuan-Huei Chang),陳永芳(Yang-Fang Chen) | |
dc.subject.keyword | 肺腺癌,CL1-0,CL1-5,核質比,雙光子螢光顯微術, | zh_TW |
dc.subject.keyword | Adenocarcinoma,CL1-0,CL1-5,nuclear-to-cytoplasmic ratio (NC ratio),Two-photon fluorescence microscopy, | en |
dc.relation.page | 42 | |
dc.identifier.doi | 10.6342/NTU201601628 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2016-08-10 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 物理學研究所 | zh_TW |
顯示於系所單位: | 物理學系 |
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