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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林璧鳳 | |
dc.contributor.author | Yi-Hsiu Chen | en |
dc.contributor.author | 陳俋秀 | zh_TW |
dc.date.accessioned | 2021-07-11T14:42:53Z | - |
dc.date.available | 2026-08-12 | |
dc.date.copyright | 2016-11-02 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-08-15 | |
dc.identifier.citation | 葉映君 (2009) 具降NF-κB轉錄活性之食材對高油飲食小鼠血糖及發炎介質的影響 國立臺灣大學微生物與生化學研究所 碩士論文
康智雄 (2009) 影響3T3-L1細胞株分泌細胞激素之食材對高油飲食小鼠的免疫調節作用 國立臺灣大學微生物與生化學研究所 碩士論文 呂學耘 (2010) 山苦瓜與共軛次亞麻油酸對氣喘模式小鼠發炎與過敏免疫反應的影響 國立臺灣大學微生物與生化學研究所 碩士論文 許永瀚 (2012) 山苦瓜乙酸乙酯萃物與奇異果對氣喘模式小鼠發炎與過敏免疫反應的影響 國立臺灣大學生化科技學系 碩士論文 黃子倩 (2013) 營養素及飲食因子對腸道免疫系統的影響 國立臺灣大學生化科技學系 碩士論文 蔡侑倫 (2013) 以急性發炎小鼠模式探討山苦瓜及其乙酸乙酯萃物對血清及肝臟發炎指標的影響 國立臺灣大學生化科技學系 碩士論文 呂學耘 (2016) 山苦瓜影響調節型T細胞進而調節腸道免疫之研究 國立臺灣大學生化科技學系 博士論文 Abraham, C. and Cho, J. H. (2009). Inflammatory bowel disease. N Engl J Med, 361(21), 2066-2078. Araki, Y., Mukaisyo, K., Sugihara, H., Fujiyama, Y. and Hattori,T. (2010). Increased apoptosis and decreased proliferation of colonic epithelium in dextran sulfate sodium-induced colitis in mice. Oncol Rep, 24(4), 869-874. Arpaia, N., Campbell, C., Fan, X., Dikiy, S., van der Veeken, J., deRoos, P. et al. (2013). Metabolites produced by commensal bacteria promote peripheral regulatory T-cell generation. Nature, 504(7480), 451-455. Arranz, A., Doxaki, C., Vergadi, E., Martinez de la Torre, Y., Vaporidi, K., Lagoudaki, E. D. et al. (2012). 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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78121 | - |
dc.description.abstract | 發炎性大腸疾病發生率增加,是目前逐受重視的飲食與健康議題。本研究室先前研究顯示山苦瓜能降低腸炎小鼠的病情,in vitro實驗指出山苦瓜乙酸乙酯萃物 (EAE) 具抗發炎功效,正丁醇萃物 (BE) 促進Foxp3+調節型T細胞 (regulatory T cell, Treg) 分化,故本研究進一步探討山苦瓜萃物EAE及BE對dextran sulfate sodium (DSS) 誘發腸炎的影響。方法為將8週齡BALB/c雄鼠分為AIN-93飼料的對照組 (Blank組)、腸炎組 (DSS組)、5%山苦瓜凍乾粉飼料的DSS/WBM組,及管餵相當DSS/WBM組所含2.4 mg/day EAE組 (DSS/EAE組)、和相當DSS/WBM組所含BE的3.2 mg/day BE 1倍組 (DSS/BE1x組)和4.8 mg/day的1.5倍組 (DSS/BE1.5x組)。分組餵飼5週後,所有的DSS組於飲水中添加3% DSS誘發腸炎,測定腸炎疾病指標 (disease activity index, DAI)包括體重流失和糞便評分。誘發7天後犧牲,測大腸長度和進行腸組織切片染色,並分離腸道固有層、皮耶氏體、腸繫膜淋巴結 (MLN)及脾臟細胞,以流式細胞儀分析Treg與CD103+樹突細胞 (dendritic cell, DC) 比率,測定血清、腸道均質液、以及MLN和脾臟細胞分泌的細胞激素含量。結果顯示,DSS/WBM組仍顯著改善腸炎指標,降低發炎反應,並提升MLN內Foxp3+ Treg比率。萃物則以DSS/EAE組顯著改善大腸長度、腸道組織受損與免疫細胞浸潤,血清IL-6以及腸組織IL-6、TNF-α、IL-1β及IFN-γ含量顯著降低,抑制MLN細胞分泌IFN-γ、IL-6與IL-10能力。補充BE在病發初期顯著降低DAI,DSS/BE1x組的腸組織TNF-α、IL-6及IFN-γ量顯著較低,DSS/BE1.5x組血清IL-6含量與MLN細胞IL-17A分泌能力顯著降低,同時,也有顯著較高的MLN與腸道黏膜固有層內CD103+ DC,以及MLN和皮耶氏體內Foxp3+ Treg和MLN內CD25+Foxp3+ Treg比率。綜合以上,山苦瓜乙酸乙酯萃物主要具抗發炎作用,而正丁醇萃物可提升腸道Treg比率,抑制腸道發炎作用。由本研究補充山苦瓜凍乾粉較萃物效果顯著,顯示山苦瓜可能透過不同成分的共同作用減緩腸炎小鼠病情。 | zh_TW |
dc.description.abstract | The incidence of inflammatory bowel disease ha been increasing worldwide. Previous study found that wild bitter melon (WBM) can ameliorate dextran sodium sulfate (DSS)-induced colitis in mice. Ethyl acetate extract (EAE) of WBM showed significant anti-inflammatory effects in vitro. N-Butanol (BE) of WBM increased the regulatory T (Treg) cell populations in primary mesenteric lymph nodes (MLN) cells. We aimed to further investigate the effects of EAE and BE on inflammatory responses and immuno-regulatory effects in DSS-induced colitis in mice. BALB/c mice were fed with AIN-93diet (Blank and DSS group), 5% WBM powder (DSS/WBM group), 2.4 mg/day EAE (DSS/EAE group), 3.2 mg/day BE (DSS/BE1x group) or 4.8 mg/day BE (DSS/BE1.5x group) by oral avage. After 5 weeks, the Blank group was fed with normal drinking water, the other groups were fed with 3% DSS in sterile water to induce colitis. Disease activity index (DAI), including body weight loss and patterns of stool, were calculated. After 7 days of DSS induction, mice were sacrificed. Colons were collected to measure the length or be stained with haematoxylin and eosin. Moreover, cells in lamina propria, Peyer’s patch, MLN and spleen were isolated. The populations of Treg and CD103+ dendritic cells (DC) was analyzed by flow cytometry. Cytokine levels in serum, colons, or secreted by mitogen-stimulated MLN or spleen cells were detected. The results showed that WBM intake significantly ameliorated colitis through inhibiting inflammation and promoting the percentage of Treg cells in MLN. EAE supplement improve colon shortening and histological evaluation of colon. Serum IL-6, colonic cytokine levels (IL-6, TNF-α, IL-1β and IFN-γ) and cytokines (IFN-γ, IL-6 and IL-10) produced by ConA-stimulated MLN cells were decreased. Oral administration of BE significantly reduced DAI during the beginning phase of DSS induction. Colonic cytokines (TNF-α, IL-6 and IFN-γ) levels were decreased in DSS/BE1x group. Serum IL-6 levels and IL-17A secreted by MLN cells were dropped in DSS/BE1.5x group. The percentage of CD103+ DC in MLN and lamina propria, Foxp3+ Treg in MLN and Peyer’s patch, as well as CD25+Foxp3+ Treg in MLN were elevated. Although both EAE and BE are beneficial to colitis, WBM is the most effective treatment on DSS mice. WBM may ameliorate DSS-induced colitis by multiple bioactive components. | en |
dc.description.provenance | Made available in DSpace on 2021-07-11T14:42:53Z (GMT). No. of bitstreams: 1 ntu-105-R03b22013-1.pdf: 6309718 bytes, checksum: 413bb05f2b467db9c2146359c88eb1ee (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | 摘要 I
目錄 III 圖目錄 VI 表目錄 VII 縮寫對照表 VIII 第一章、緒論 1 第一節 文獻回顧 1 一、 腸道免疫系統 1 (一) 腸道免疫系統組成 1 (二) 腸道表皮屏障 2 (三) 腸道免疫系統的免疫細胞 3 二、 發炎性腸道疾病 9 (一) 簡介及定義 9 (二) 免疫反應與發炎性腸道疾病 10 (三) 調節型T細胞與發炎性腸道疾病 11 (四) 實驗動物模式 12 (五) DSS誘發腸炎可能機制 12 三、 飲食因子與免疫調節 13 (一) 維生素A 13 (二) 益生菌 13 (三) 中草藥與食材 14 四、 苦瓜 15 (一) 苦瓜簡介 15 (二) 苦瓜與山苦瓜的抗發炎功效 16 第二節 研究動機與目的 21 第二章、材料與方法 22 一、 山苦瓜萃物之製備 22 二、 實驗設計 23 三、 動物飼養 24 四、 DSS誘發腸炎 25 五、 血清樣本收集 26 六、 大腸組織樣本處理 26 七、 淋巴細胞分離與培養 29 八、 總蛋白質含量分析 31 九、 細胞激素含量分析 32 十、 免疫細胞表型分析 34 十一、 統計方法 36 第三章、結果與討論 37 一、 山苦瓜萃物對小鼠生長情形與DSS誘發腸炎之影響 37 (一) 山苦瓜萃物對小鼠生長情形與攝食量的影響 37 (二) 山苦瓜萃物對疾病指標與體重流失的影響 38 (三) 山苦瓜萃物對組織重的影響 40 (四) 山苦瓜萃物對腸炎小鼠腸道組織的影響 42 二、 山苦瓜萃物對DSS誘發腸炎小鼠體內細胞激素之影響 45 (一) 山苦瓜萃物對腸炎小鼠組織發炎激素的影響 45 (二) 山苦瓜萃物對腸炎小鼠MLN細胞分泌細胞激素的影響 47 (三) 山苦瓜萃物對腸炎小鼠脾臟細胞分泌細胞激素的影響 47 (四) 山苦瓜萃物對腸炎小鼠血清IL-6含量的影響 50 三、 山苦瓜萃物對DSS誘發腸炎小鼠免疫細胞族群之影響 51 (一) 山苦瓜萃物對腸炎小鼠腸道與脾臟細胞數目的影響 51 (二) 山苦瓜萃物對腸炎小鼠腸道與脾臟調節型T細胞比率的影響 52 (三) 山苦瓜萃物對腸炎小鼠腸道CD103+樹突細胞比率的影響 54 (四) 山苦瓜萃物對腸炎小鼠腸道CD103+樹突細胞比率的影響 55 第四章、綜合討論與結論 56 第一節 綜合討論 56 一、 DSS誘發腸炎模式建立 56 二、 山苦瓜乙酸乙酯萃物減緩DSS誘發腸炎之可能機制 57 三、 山苦瓜正丁醇萃物具提升DSS誘發腸炎小鼠腸道免疫調節功能 之潛力 58 四、 山苦瓜對巨噬細胞的影響 60 五、 不同萃物對腸炎免疫相關指標的作用 61 第二節 結論 63 附錄 64 參考文獻 65 | |
dc.language.iso | zh-TW | |
dc.title | 山苦瓜萃物對 DSS 誘發腸炎小鼠免疫反應的影響 | zh_TW |
dc.title | Effects of wild bitter melon extracts on the immune responses of DSS-induced colitis in mice | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 江伯倫,江孟燦,謝佳倩,洪永翰 | |
dc.subject.keyword | 腸炎,山苦瓜萃物,抗發炎,調節型T細胞, | zh_TW |
dc.subject.keyword | wild bitter melon,colitis,anti-inflammation,regulatory T cells, | en |
dc.relation.page | 76 | |
dc.identifier.doi | 10.6342/NTU201602530 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2016-08-16 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
dc.date.embargo-lift | 2026-08-12 | - |
顯示於系所單位: | 生化科技學系 |
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