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DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳億乘 | |
dc.contributor.author | Yi-De Wu | en |
dc.contributor.author | 吳奕德 | zh_TW |
dc.date.accessioned | 2021-07-11T14:39:49Z | - |
dc.date.available | 2022-02-21 | |
dc.date.copyright | 2017-02-21 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-01-26 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78018 | - |
dc.description.abstract | 沙門氏菌感染症(salmonellosis)在肉雞產業中不只造成飼主的經濟損失,更因為和食品的交叉污染而產生公共食品安全上的危害。本實驗為了調查海藻糖(trehalose)在肉雞腸道中抗沙門氏菌感染的能力,故將海藻糖添加於白肉雞飼糧中。在耐受性實驗中,添加最高到10%的海藻糖並不會對雞隻的生長性能和死亡率產生影響(p>0.05),然而,在鼠傷寒沙門氏菌(Salmonella Typhimurium)(S.T.)的攻毒實驗中,相對於單純攻毒的組別(0%Treh+S.T.),添加1、3%海藻糖的組別在飼料換肉率(feed conversion ratio)(feed intake/weight gain)上有較好的趨勢(p>0.05),反觀添加5%海藻糖的組別則有顯著改善(p<0.05)且和控制組(0%Treh)相同(p>0.05)的水準。雖然在盲腸腸道菌相觀察的結果中,海藻糖的添加顯著降低(p<0.05)肉雞盲腸內S.T.的數量,但此統計上的差異不足以讓攻毒雞隻產生不同的臨床症狀,然而,盲腸中較低的S.T.數量可以降低雞隻因為屠宰掏肛而交叉污染到屠體的情形。另外,相對於單純攻毒的組別,添加海藻糖顯著提升(p<0.05)肉雞各腸段總乳酸桿菌(lactobacillus)的數量和迴腸內乙酸(acetic acid)的含量。再者,肉雞血清中的丙氨酸轉氨酶(alanine aminotransferase)、白蛋白和球蛋白比(albumin/
globulin)、三酸甘油酯(triglyceride)和尿酸(uric acid)都顯著的受到S.T.攻毒的影響(p<0.05),而添加3、5%海藻糖的組別則有改善(p<0.05)的結果。透過盲腸組織切片染色(H&E staining)的觀察,海藻糖的添加減緩了來自S.T.攻毒造成的腸道固有層免疫細胞浸潤的情形,同時也維持了腸道上皮細胞的完整性。而透過發炎基因表現的測定,結果顯示添加5%海藻糖後,減緩盲腸發炎的現象來自於抑制toll-like receptor 4 – nuclear factor kappa-light-chain-enhancer of activated B cells(TLR4-NFκB)路徑,如降低盲腸組織中(p<0.05)interleukin 1 beta(IL-1β)、lipopolysaccharide -induced tumor necrosis factor alpha factor(LITAF)的基因表現,另外,添加5%海藻糖也提升了(p<0.05)interleukin 10(IL-10)、interferon alpha(IFNα)等抑發炎基因的表現。 綜合上述,海藻糖對雞隻產業不僅有成為益生質的潛力、提升雞隻的抗發炎能以外,更降低消費者接觸S.T.的可能性,以提升食品安全性。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-07-11T14:39:49Z (GMT). No. of bitstreams: 1 ntu-106-R03626025-1.pdf: 9812808 bytes, checksum: 251a95efb6076668c87da9071f342339 (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | I. Introduction………………………………………...…………………….. 1
II. Literature review…………………………………………………………. 6 2.1 Trehalose…………………………………………………………………. 6 2.11 Discovery and research on trehalose…………….……………………… 6 2.12 Structure and properties of trehalose…………….………………… 7 2.13 Distribution and role of trehaose in nature………………… 8 2.14 Preparation of trehalose………………………….……………………….. 10 2.15 Application of trehalose in current studies……………… 13 2.2 Salmonella……………………………………….……………………….. 16 2.21 Classification of Salmonella……………………..……………………….. 16 2.22 Characteristic of Salmonella……………………..……………………….. 17 2.23 Epidemiology and infection route of salmonella..… 21 2.24 Pathogenesis of Salmonella……………………...……………………….. 25 2.25 Intestinal epithelial cell (IEC) barrier and immune response of Salmonellosis…………………… 31 2.3 Prebiotics…………………………………………...…………………….. 36 2.3.1 What are prebiotics?........................................... 36 2.3.2 Prebiotic used in the poultry industry………………………… 40 2.3.3 Possibility of trehalose as a prebiotic in chicken industry……………….. 46 III. Materials & methods…………………………….……………………….. 50 3.1 Trehalose…………………………………………………………………. 50 3.2 Salmonella Typhimurium and Lactobacillus johnsonii……………...…... 52 3.3 Animal and treatment…………………………………………………….. 53 3.4 Exp I: The tolerance test of trehalose on broilers…………… 61 3.5 Exp II: The antibacterial tests of trehalose on broilers………..………….. 62 3.6 Sample collections……………………………….……………………….. 63 3.7 Isolation and identification of bacteria (real-time PCR)………………..... 64 3.8 Serum biochemistry analysis…………………….……………………….. 67 3.9 Caecal histopathology and staining……………...…………………… 67 3.10 Inflammation-related cytokines analysis (real-time PCR).………………. 68 3.11 Analysis in volatile fatty acids in intestinal chymus……………….…….. 71 3.12 Statistic analysis………………………………………………………….. 72 IV. Results & discussions…………………………………………………….. 73 4.1 Exp I: The tolerance test of trehalose on broilers..……………………….. 73 4.2 Exp II: The antibacterial tests of trehalose on broilers challenged by Salmonella Typhimurium……………………… 77 4.2.1 Effects of trehalose supplementation on growth performance of broilers challenged by Salmonella Typhimurium Serum biochemistry of broilers………. 77 4.2.2 Effects of trehalose supplementation on Salmonella Typhimurium and total lactobacillus in intestine or feces of broilers challenged by Salmonella Typhimurium Caecal tissue histopathology…………………. 79 4.2.3 Effects of trehalose supplementation on serum biochemical values and caecal-tissue histopathology of broilers challenged by Salmonella Typhimurium…………………… 82 4.2.4 Anti-inflammatory mechanism of trehalose supplementation on caecal tissues of broilers challenged by Salmonella Typhimurium………………………………… 86 4.2.5 Effects of trehalose supplementation on volatile fatty acid levels in caecal tissue of broilers challenged by Salmonella Typhimurium……………………………………………… 91 V. Conclusion………………………………………………………………... 110 Reference ……………………………………………………………………….. 113 Appendix ……………………………………………………………………….. 128 | |
dc.language.iso | en | |
dc.title | 探討肉雞飼糧中添加海藻糖對鼠傷寒沙門氏菌攻毒模式下的
影響 | zh_TW |
dc.title | Investigation of trehalose supplementation on Salmonella
Typhimurium infection in broilers | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 余碧,周崇熙,黃惠君,張元衍 | |
dc.subject.keyword | 海藻糖,鼠傷寒沙門氏菌,乳酸菌,白肉雞,食品安全, | zh_TW |
dc.subject.keyword | trehalose,Salmonella Typhimurium,lactobacillus,broilers,food safety, | en |
dc.relation.page | 129 | |
dc.identifier.doi | 10.6342/NTU201700237 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-01-26 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 動物科學技術學研究所 | zh_TW |
顯示於系所單位: | 動物科學技術學系 |
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