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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林?輝 | |
dc.contributor.author | Ching-Yun Chen | en |
dc.contributor.author | 陳靖昀 | zh_TW |
dc.date.accessioned | 2021-07-11T14:39:01Z | - |
dc.date.available | 2022-03-20 | |
dc.date.copyright | 2017-08-29 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-06-30 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/77987 | - |
dc.description.abstract | 組織工程是一門跨領域科學,結合細胞、支架、生長環境等因子,從生物醫學及工程的角度,實現重建及再生人類組織或器官之學科。因應老年化社會到來,骨組織工程相關研究受到高度重視,與年齡之骨科疾病及骨缺損,已然成為重要的公共健康問題,重建及再生骨組織的研究是刻不容緩的議題。細胞治療技術則是建構在組織工程的基礎上,提供骨組織再生醫學相關研究新穎並具潛力之解決方法,然而培養適合臨床使用且具有功能性的細胞或組織,仍是相當艱難的挑戰。
本研究提出一種可培養類硬骨組織團塊(bone-like tissues)的技術,由人類成骨細胞(hOBs)、鈣交聯褐藻酸生物支架(Ca-Alginate scaffolds)和自組裝生物反應系統(self-designed bioreactor system)所建構的平台,提供硬骨(bone)自體移植的功能性類硬骨組織團塊。本技術所建立之生物反應器為封閉式迴流系統,提供基礎細胞培養液,避免更換培養液可能造成之汙染,更可以回饋細胞所分泌之訊息因子;鈣交聯褐藻酸生物支架在系統中提供細胞生長環境,輔助成骨細胞及間葉幹細胞增生、分化並維持功能性。 針對此培養技術評估,就安全性考量,客製化自體移植避免引發免疫排斥之風險,也避免等待適合捐贈者耗費時間及心理煎熬;就便利性考量,自組裝生物反應系統容易架設;就成本考量,系統所需器皿及管路,皆為已滅菌拋棄式耗材,不會造成經濟上負擔。此培養技術結合安全性、便利性及低成本等三大優勢,期許未來能夠提供病人安全有效並兼顧生活品質的選擇。 | zh_TW |
dc.description.abstract | Tissue engineering is an interdisciplinary field that combines the principles of biomedical science and engineering with the goal of achieving human tissue regeneration or reconstruction. Due to the elderly people represented a growing share of the worldwide population, age-related orthopedic disorders and bone defects have become a critical public health issue. Cell-based therapy on the basis of tissue engineering provides a novel and potential solution to bone regenerative research; however, to accomplish real functional cells or tissues for clinical use is still a tough challenge.
This study presented a platform, which were composed of cells (hOBs), Ca-Alginate scaffolds, and self-designed bioreactor system, to form bone-like tissue for bone autogenic transplantation. Since the self-designed bioreactor is a closed-process perfusion system, it not only offers the basal cell culture media for nutrient transportation, but also prevents cell contamination resulting from media replacement. Moreover, the secreted cytokine and growth factor can work directly and feedback on the cells in this dynamic system. The Ca-Alginate scaffold in this system supplied as a cell niche for cell ingrowth, proliferation, differentiation, and function maintenance. Concern for safety, personalized bone-like tissues allow autogenic transplantation without the risk of immune reactions. In addition, the system is easily to assemble and serves as a convenient tool for researchers or medical doctors. Furthermore, all components of the apparatus are disposable, and the price is affordable for patients. Consequently, this strategy can be applied on cell therapy and opens new avenues for surgical interventions to overcome bone disorders. | en |
dc.description.provenance | Made available in DSpace on 2021-07-11T14:39:01Z (GMT). No. of bitstreams: 1 ntu-106-D97548017-1.pdf: 6133395 bytes, checksum: 5b6b2df8f0e96a439f43bafa9bbf4a53 (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 口試委員會審定書 i
誌謝 ii 中文摘要 iii ABSTRACT iv CONTENTS v LIST OF FIGURES viii LIST OF TABLES x ABBREVIATIONS xi Chapter 1 Introduction 1 1.1 Bone Physiology and Structure 1 1.1.1 Bone mineral density (BMD) 2 1.1.2 Bone mineralization 2 1.2 Bone Disorders 5 1.3 Age-Related Bone Disorders 6 1.4 Tissue Engineering (TE) 7 1.4.1 Bone TE 8 1.5 Scaffolds 10 1.5.1 Difference between 2D and 3D Culture 11 1.6 Bioreactor System 13 1.7 Purpose of Study 14 Chapter 2 Theoretical Basis 16 2.1 Biological Approach for Bone Regeneration and Reconstruction 16 2.2 Alginate Polysaccharide 18 2.2.1 Cross-linking mechanism of Ca-Alginate scaffolds 18 2.2.2 Metal ion chelating method of Ca-Alginate scaffolds 19 2.3 Bioreactor System 22 Chapter 3 Materials and Methods 24 3.1 Experimental Materials 24 3.2 Experimental Methods 25 3.2.1 Ca-Alginate scaffold fabrication and preparation 25 3.2.2 hOBs isolation and expansion 25 3.2.3 Development of functionally-closed process bioreactor system 26 3.2.4 Characterization of Ca-Alginate scaffolds 26 3.2.5 Cell seeding method 26 3.2.6 Cell proliferative quantification 29 3.2.7 SEM and EDX analysis 29 3.2.8 Cell cluster size distribution 29 3.2.9 Q-PCR quantification 31 3.2.10 Confocal microscope 31 3.2.11 3D micro-computed tomography (micro-CT) 32 3.2.12 Statistical analysis 32 Chapter 4 Results 33 4.1.1 Characterization of Ca-Alginate scaffolds and hOBs 33 4.1.2 Doubling time and seeding efficiency of hOBs 34 4.1.3 Live/Dead staining of bone-like tissues in Ca-Alginate scaffolds 38 4.1.4 SEM morphology and EDX Analysis 38 4.1.5 Size distribution of bone-like tissues 39 4.1.6 mRNA expression levels of bone-like tissues 43 4.1.7 Biomineralization of bone-like tissues 44 4.1.8 Volumetric bone mass density and bone volume fraction 44 Chapter 5 Discussions 49 Chapter 6 Conclusion 54 REFERENCES 55 | |
dc.language.iso | en | |
dc.title | 利用自組裝之生物反應系統培養類硬骨組織團塊應用於骨組織工程 | zh_TW |
dc.title | Forming Bone-like Tissues in the Self-designed Bioreactor System for Bone Tissue Engineering | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-2 | |
dc.description.degree | 博士 | |
dc.contributor.coadvisor | 孫瑞昇 | |
dc.contributor.oralexamcommittee | 郭士民,張淑真,謝文元 | |
dc.subject.keyword | 骨組織工程,功能性類硬骨組織團塊,自組裝生物反應系統,細胞治療, | zh_TW |
dc.subject.keyword | bone tissue engineering,bone-like tissues,self-designed bioreactor system,cell therapy, | en |
dc.relation.page | 62 | |
dc.identifier.doi | 10.6342/NTU201701229 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-06-30 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
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