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標題: | 利用移動式漫反射光譜系統定量子宮頸癌前病變之組織光學參數 Quantifying In-Vivo Tissue Optical Parameters of Precancerous Cervical Lesions using a Portable Reflectance Spectroscopy System |
作者: | Tsan-Hsueh Huang 黃贊學 |
指導教授: | 宋孔彬(KUNG-BIN SUNG) |
關鍵字: | 漫反射光譜,蒙地卡羅法,黏膜組織,散射係數,吸收係數,癌前病變, Diffuse Reflectance Spectroscopy,Monte Carlo method,Mucosal tissue,scattering coefficient,absorption coefficient,precancerous lesions, |
出版年 : | 2017 |
學位: | 碩士 |
摘要: | 臨床上,子宮頸組織病變與否需仰賴專業訓練之醫師透過陰道鏡(Colposcopy)放大檢視可疑的部位,若為輕微者,以不具侵入性之抹片方式進行表層組織取樣; 若較為嚴重者,則以具侵入性之切片方式進行組織取樣,最後皆須透過病理醫師進行樣本診斷。由於需經病理醫師判定,故其結果較為主觀且過程相當耗時。本研究希望透過即時非侵入性光學診斷方式,萃取出可疑部位之組織光學參數,藉由分析參數差異告知醫師組織變化情形,提供即時的醫療資訊,除能協助醫師診斷外,以期提升醫院診斷效率。
本實驗室利用蒙地卡羅演算法模擬光子在不同散射及吸收特性組織內之行進情形,藉此得到蒙地卡羅順向模擬光譜,再透過順向模擬光譜進行組織漫反射光譜校正,目前採用光源光纖與六根不同距離(Source to Detection Separation, SDS)光纖之分析模式,並以兩種不同直徑的擬組織散射小球(Polystyrene)及血紅素調配而成之仿體進行校正,最後利用光譜擬合工具擬合校正後之子宮頸漫反射光譜,萃取出組織特性之相關光學參數,如:散射係數、吸收係數、血氧飽和、血紅素濃度、膠原蛋白濃度及上皮層厚度等。 本研究基於先前實驗室莊閔傑所架設之臨床可移動式光譜儀系統[1]結合多根多模光纖提供光源光纖與不同距離之偵測光纖量測子宮頸黏膜組織,接著利用逆向光譜擬合工具萃取組織光學參數,旨為探討正常與病變組織間漫反射光譜與光學參數的差異與定量分析。 本系統已通過本校醫學院附設醫院科學研究與倫理委員會核可,其電性安全也符合醫工部之規範。預計本系統未來可建立子宮頸黏膜組織之光學參數與光譜特徵資料庫,作為判斷癌前病變進程的依據之一。 In clinical, cervical tissue lesions or not need to rely on professional training physician through colposcopy to enlarge the view of suspicious sites. In the case of minor, the surface tissue is sampled in a non-invasive manner such as pap test. If more severe, the tissue is sampled in a invasive manner, and the sample is diagnosed by the pathologist. Due to the need to determine by the pathologist, the results are more subjective and the process is quite time-consuming. This study hope to extract the tissue optical parameters of the suspected sites through real-time non-invasive optical diagnosis. By analyzing the differences in parameters inform physician organizational changes and provide real-time medical information. In addition to assisting physician diagnosis, also improve the hospital diagnosis efficiency. In this laboratory, the Monte Carlo algorithm is used to simulate the traveling conditions of photons in different scattering and absorption properties. The Monte Carlo simulation spectra are obtained and used to correct the diffuse reflectance spectra of the tissue. The analysis mode of source fiber and six different Source to Detection Separation (SDS) fiber is used to calibrate with phantoms consist of different concentration of microspheres (Polystrene) and hemoglobin. The reverse spectral fitting tools fit the corrected diffuse reflectance spectra of the cervix and extract the relevant optical parameters of the tissue properties such as scattering coefficient, absorption coefficient, oxygen saturation, hemoglobin concentration, collagen concentration and epithelial thickness. This study is based on the previous laboratory Zhuang Minjie set up by the portable reflectance spectroscopy system combined with multiple multi-mode fiber to provide light source fiber with different distance detection fiber measurement of cervical mucosa. The optical parameters of the tissue were extracted by the reverse spectral fitting tool. The purpose of this study is to explore the difference and quantitative analysis between the diffuse reflectance spectrum and the optical parameters between normal and precancerous lesions tissues. The system has been approved by the Hospital Scientific Research and Ethics Committee of NTUH. Its electrical safety is also in line with the Ministry of Medical Standards. It is expected that the system can establish the optical parameters and spectral characteristics database of cervical mucosa in the future as one of the basis for judging the process of precancerous lesions. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/77969 |
DOI: | 10.6342/NTU201701766 |
全文授權: | 有償授權 |
顯示於系所單位: | 生醫電子與資訊學研究所 |
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