Please use this identifier to cite or link to this item:
Fabrication and Characterization of Asymmetric Two-layer Membranes Bonded by Bioadhesives
|Publication Year :||2016|
|Abstract:||緻密膜材機械強度高，電紡纖維膜利於質傳及細胞貼附但機械強度低，若能結合兩者優點製成非對稱雙層膜材，未來可將其用於牙科的引導骨再生(guided bone regeneration)手術，即以緻密膜作為屏障膜，隔絕牙齦組織，同時以纖維膜攜帶生長因子促進骨骼生長，達到雙重功效。本研究使用甲/乙酸作為溶劑，利用溶劑澆鑄法與靜電紡絲法分別製備出聚己內酯緻密膜材與聚己內酯/動物明膠(P/G)電紡纖維膜材，為了增加膜材間黏著強度，先以電漿處理對緻密膜材表面進行改質，再以動物明膠/戊二醛溶液作為黏著劑黏合此兩種膜材而成為非對稱雙層膜材。
本研究利用掃描式電子顯微鏡觀察膜材型態，並以拉伸性質測定比較膜材間機械性質的差異；以傅立葉紅外線光譜儀、X光光電子能譜儀、親水性質測定了解以電漿處理不同時間的緻密膜材表面特性，也證實P/G兩成分混合於纖維膜中；再以膜材黏著劑之成膠時間與剝離強度測定(T peel test)證實緻密膜經電漿處理後能大幅提高兩種膜材間的黏著強度，也藉由並改變黏著劑濃度、塗佈轉速、電漿處理時間等參數找出最佳的黏合參數；再以崩解性質測試與細胞毒性測試證實本研究製備的非對稱雙層膜材於水中能維持不分離且無明顯生物毒性。未來希望本研究所製備之非對稱雙層膜材能進一步應用於牙科的引導骨再生手術。
Dense films have high mechanical strength while fibrous mats can promote mass transfer efficiency and cell attachment. However, fibrous mats have lower mechanical strength. In consideration of these factors, a dense film and a fibrous mat can be bonded together to form an asymmetric two-layer membrane. It may be utilized as a dental membrane for guided bone regeneration, the dense layer of an asymmetric two-layer membrane can act as a barrier membrane to block connective tissue and epithelium while the fibrous layer can carry growth factors and thus facilitate bone regeneration. In this research, a mixture of formic acid/acetic acid was chosen to be the solvent to fabricate polycaprolactone (PCL) dense films and polycaprolactone/gelatin (P/G) fibrous mats by solvent casting and electronspinning respectively. To increase the bonding strength between two layers, the PCL films were first surface-modified by plasma treatment and then gelation/glutaraldehyde solution was used as an adhesive to bond the dense layer (PCL film) and the fibrous layer (P/G mats), to form an asymmetric two-layer membrane.
In this research, scanning electron microscope was used to observe the morphology of membranes; tensile tests were carried out to compare the difference between dense films and fibrous mats. Through Fourier transform infrared (FT-IR) spectroscopy, X-ray photoelectron spectroscopy (XPS) and water contact angle measurements, the surface characteristic of PCL films with different plasma exposure times were determined and the composition of P/G fibrous mats was also confirmed. The bonding strength between two layers was increased by treating PCL film with plasma exposure, which was confirmed by T peel test. Finally, a gelatin/glutaraldehyde solution with different optimal experimental parameters were selected for bonding two layers. Degradation and cytotoxicity tests confirmed that the asymmetric two-layer membranes maintained well-adhered in wet condition and possessed good cytocompatibility. It is expected that the above-mentioned asymmetric two-layer membranes can be further applied in guided bone regeneration.
|Appears in Collections:||化學工程學系|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.