請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/77958
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝學真(Hsyue-Jen Hsieh) | |
dc.contributor.author | Hsin-Yi Huang | en |
dc.contributor.author | 黃欣儀 | zh_TW |
dc.date.accessioned | 2021-07-11T14:38:18Z | - |
dc.date.available | 2022-08-29 | |
dc.date.copyright | 2017-08-29 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-07-26 | |
dc.identifier.citation | Nerem, R.M. and Sambanis, A., Tissue engineering: from biology to biological substitutes. Tissue Engineering, 1995. 1(1): 3-13.
Sasmazel, H.T., Gumusderelioglu, M., Gurpinar, A. and Onur, M.A., Comparison of cellular proliferation on dense and porous PCL scaffolds. Bio-Medical Materials and Engineering, 2008. 18(3): 119-128. Angelova, N. and Hunkeler, D., Rationalizing the design of polymeric biomaterials. Trends in Biotechnology, 1999. 17(10): 409-421. Retzepi, M. and Donos, N., Guide Bone Regeneration: biological principle and therapeutic applications. Clinical Oral Implants Research, 2010. 21(6): 567-576. Xue, J., He, M., Liang, Y., Crawford, A., Coates, P., Chen, D., Shi, R. and Zhang, L., Fabrication and evaluation of electrospun PCL-gelatin micro-/nanofiber membranes for anti-infective GTR implants. Journal of Materials Chemistry B, 2014. 2(39): 6867-6877. Bottino, M.C., Thomas, V., Schmidt, G., Vohra, Y.K., Chu, T.-M.G., Kowolik, M.J. and Janowski, G.M., Recent advances in the development of GTR/GBR membranes for periodontal regeneration-A materials perspective. Dental Materials, 2012. 28(7): 703-721. Neel, E.A.A., Chrzanowski, W., Salih, V.M., Kim, H.-W. and Knowles, J.C., Tissue engineering in dentistry. Journal of Dentistry, 2014. 42(8): 915-928. Barnes, C.P., Sell, S.A., Boland, E.D., Simpson, D.G. and Bowlin, G.L., Nanofiber technology: Designing the next generation of tissue engineering scaffolds. Advanced Drug Delivery Reviews, 2007. 59(14): 1416-1433. Lee, L., Yoo, J.J., Atala, A. and Lee, S.J., The effect of controlled release of PDGF-BB from heparin-conjugated electrospun PCL/gelatin scaffolds on cellular bioactivity and infiltration. Biomaterials, 2012. 33(28): 6709-6720. Agarwal, S., Wendorff, J.H. and Greiner, A., Use of electrospinning technique for biomedical applications. Polymer, 2008. 49(26): 5603-5621. Li, L., Qian, Y., Jiang, C., Lv, Y., Liu, W., Zhong, L., Cai, K., Li, S. and Yang, L., The use of hyaluronan to regulate protein adsorption and cell infiltration in nanofibrous scaffolds. Biomaterials, 2012. 33(12): 3428-3445. Rujitanaroj, P.-O., Pimpha, N. and Supaphol, P., Wound-dressing materials with antibacterial activity from electrospun gelatin fiber mats containing silver nanoparticles. Polymer, 2008. 49(21): 4723-4732. Ushiki, T., Collagen fibers, reticular fibers and elastic fibers. A comprehensive understanding from a morphological viewpoint. Archives of Histology and Cytology, 2002. 65(2): 109-126. Formhals, A., Process and apparatus for preparing artificial threads. 1934: US patent 1975504 A. Baji, A., Mai, Y.-W., Wong, S.C., Abtahi, M. and Chen, P., Electrospinning of polymer nanofibers: Effects on oriented morphology, structures and tensile properties. Composites Science and Technology, 2010. 70(5): 703-718. Baumgarten, P.K., Electrostatic Spinning of Acrylic Microfibers. Journal of Colloid and Interface Science, 1971. 36(1): 71-79. Shin, Y.M., Hohman, M.M., Brenner, M.P. and Rutledge, G.C., Experimental characterization of electrospinning: the electrically forced jet and instabilities. Polymer, 2001. 42(25): 9955-9967. Zhang, C.L. and Yu, S.H., Nanoparticles meet electrospinning: recent advances and future prospects. Chemical Society Reviews, 2014. 43(13): 4423-4448. Ducheyne, P., Comprehensive biomaterials. 2011, Amsterdam ; Boston: Elsevier. Tao, J. and Shivkumar, S., Molecular weight dependent structural regimes during the electrospinning of PVA. Materials Letters, 2007. 61(11-12): 2325-2328. Gupta, P., Elkins, C., Long, T.E. and Wilkes, G.W., Electrospinning of linear homopolymers of poly(methyl methacrylate): exploring relationships between fiber formation, viscosity, molecular weight and concentration in a good solvent. Polymer, 2005. 46(13): 4799-4810. Zong, X., Kim, K., Fang, D. and Ran, S., Structure and process relationship of electrospun bioabsorbable nanofiber membranes. Polymer, 2002. 43(16): 4403-4412. Sukigara, S., Gandhi, M., Ayutsede, J., Micklus, M. and Ko, F., Regeneration of Bombyx mori silk by electrospinning-part 1: processing parameters and geometric properties. Polymer, 2003. 44(13): 5721-5727. Deitzel, J.M., Kleinmeyer, J., Harris, D. and Tan, N.C.B., The effect of processing variables on the morphology of electrospun nanofibers and textiles. Polymer, 42(1): 261-272. Uyar, T., Besenbacher, F., Electrospinning of uniform polystyrene fibers: The effect of solvent conductivity. Polymer, 2008. 49(24): 5336-5343. Haghi, A.K. and Akbari, M., Trends in electrospinning of natural nanofibers. Physica Status Solidi (a), 2007. 204(6): 1830-1834. Zhang, C.X., Yuan, X.Y., Wu, L.L., Han, Y. and Sheng, J., Study on morphology of electrospun poly(vinyl alcohol) mats. European Polymer Journal, 2005. 41(3): 423-432. Deitzel, J.M., Kleinmeyer, J., Harris, D. and Tan, N.C.B., The effect of processing variables on the morphology of electrospun nanofibers and textiles. Polymer, 2001. 42(1): 261-272. Mo, X.M., Xu, C.Y., Kotaki, M. and Ramakrishna, S., Electrospun P(LLA-CL) nanofiber: a biomimetic extracellular matrix for smooth muscle cell and endothelial cell proliferation. Biomaterials, 2004. 25(10): 1883-1890. Buchko, C.J., Chen, L.C., Shen, Y. and Martin, D.C., Processing and microstructural characterization of porous biocompatible protein polymer thin films. Polymer, 1999. 40(26): 7397-7407. Vrieze, S.D., Camp, T.V., Nelvig, A., Hagstrom, B., Westbroek, P. and Clerck, K.D., The effect of temperature and humidity on electrospinning. Journal of Materials Science, 2009. 44(5): 1357-1362. Nezarati, R.M., Eifert, M.B. and Cosgriff-Hernandez, E., Effects of Humidity and Solution Viscosity on Electrospun Fiber Morphology. Tissue Engineering. Part C, Methods, 2013. 19(10): 810-819. Theron, S.A., Yarin, A.L., Zussman, E. and Kroll, E., Multiple jets in electrospinning: experiment and modeling. Polymer, 2005. 46(9): 2889-2899. Niu, H.T., Lin, T. and Wang, X.G., Needleless Electrospinning. I. A Comparison of Cylinder and Disk Nozzles. Journal of Applied Polymer Science, 2009. 114(6): 3524-3530. Moghe, A.K. and Gupta, B.S., Co-axial electrospinning for nanofiber structures: preparation and applications. Polymer Reviews, 2008. 48(2): 353-377. Pham, Q.P., Sharma, U. and Mikos, A.G., Electrospinning of polymeric nanofibers for tissue engineering applications: A review. Tissue Engineering, 2006. 12(5): 1197-1211. Liang, D.-H., Hsiao, B.S. and Chu, B., Functional electrospun nanofibrous scaffolds for biomedical applications. Advanced Drug Delivery Reviews, 2007. 59 (14): 1392-1412. Xu, J., Zhang, J., Gao, W., Liang, H., Wang, H. and Li, J., Preparation of chitosan/PLA blend micro/nanofibers by electrospinning. Materials Letters, 2009. 63(8): 658-660. Lee, J., Tae, G., Kim, Y.H., Park, I.S., Kim, S.-H. and Kim, S.H., The effect of gelatin incorporation into electrospun poly(L-lactide-co-ε-caprolactone) fibers on mechanical properties and cytocompatibility. Biomaterials, 2008. 29(12): 1872-1879. 宋信文、陳松青,生醫材料簡介,生物產業技術概論,第33-58頁,國立清華大學,2010。 Cipitria, A., Skelton, A., Dargaville,, T.R., Dalton, P.D. and Hutmacher, D.W., Design, fabrication and characterization of PCL electrospun scaffolds-a review. Journal of Materials Chemistry, 2011. 21(26): 9419-9453. Luong-Van, E., Grøndahl, L., Chua, K.N., Leong, K.W., Nurcombe, V. and Cool, S.M., Controlled release of heparin from poly(ε-caprolactone) electrospun fibers. Biomaterials, 2006. 27(9): 2042-2050. Zamani, M., Morshed, M., Varshosaz, J. and Jannesari, M., Controlled release of metronidazole benzoate from poly ε-caprolactone electrospun nanofibers for periodontal diseases. European Journal of Pharmaceutics and Biopharmaceutics, 2010. 75(2): 179-185. Lee, K.H., Kim, H.Y., Khil, M.S., Ra, Y.M. and Lee, D.R., Characterization of nano-structured poly(ε-caprolactone) nonwoven mats via electrospinning. Polymer, 2003. 44(4): 1287-1294. Ghasemi-Mobarakeh, L., Prabhakaran, M.P., Morshed, M., Nasr-Esfahani, M.H. and Ramakrishna, S., Bio-functionalized PCL nanofibrous scaffolds for nerve tissue engineering. Materials Science and Engineering: C, 2010. 30(8): 1129-1136. Jayakumar, R., Prabaharan, M., Nair, S.V. and Tamura, H., Novel chitin and chitosan nanofibers in biomedical applications. Biotechnology Advances, 2010. 28(1): 142-150. Croisier, F. and Jérôme, C., Chitosan-based biomaterials for tissue engineering. European Polymer Journal, 2013. 49(4): 780-792. Kubota, N., Tatsumoto, N., Sano, T. and Toya, K., A simple preparation of half N-acetylated chitosan highly soluble in water and aqueous organic solvents. Carbohydrate Research, 2000. 324(4): 268-274. Pakravan, M., Heuzey, M.-C. and Ajji, A., A fundamental study of chitosan/PEO electrospinning. Polymer, 2011. 52(21): 4813-4824. Lee, S.J., Heo, D.N., Moon, J.-H., Ko, W.-K., Lee, J.B., Bae, M.S., Park, S.W., Kim, J.E., Lee, D.H., Kim, E.-C., Lee, C.H. and Kwon, I.K., Electrospun chitosan nanofibers with controlled levels of silver nanoparticles. Preparation, characterization and antibacterial activity. Carbohydrate Polymers, 2014. 111: 530-537. Liu, X., Smith, S.A., Hu, J. and Ma, P.X., Biomimetic nanofibrous gelatin/apatite composite scaffolds for bone tissue engineering. Biomaterials, 2009. 30(12): 2252-2258. Liu, Y., Liu, X.H. and Wang, X., Biomimetic Synthesis of Gelatin Polypeptide-Assisted Noble-Metal Nanoparticles and Their Interaction Study. Nanoscale Research Letters, 2011. 6(22): 1-11. Okhawilai, M., Rangkupan, R., Kanokpanont, S. and Damrongsakkul, S., Preparation of Thai silk fibroin/gelatin electrospun fiber mats for controlled release applications. International Journal of Biological Macromolecules, 2010. 46(5): 544-550. Le Trong, I., McDevitt, T.C., Nelson, K.E., Stayton, P.S. and Stenkamp, R.E., Structural characterization and comparison of RGD cell-adhesion recognition sites engineered into streptavidin. Acta Crystallographica Section D-Biological Crystallography, 2003. 59: 828-834. Gu, S.-Y., Wang, Z.-M., Ren, J. and Zhang, C.-Y., Electrospinning of gelatin and gelatin/poly(L-lactide) blend and its characteristics for wound dressing. Materials Science and Engineering: C, 2009. 29(6): 1822-1828. Son, W.K., Youk, J.H., Lee, T.S. and Park, W.H., The effects of solution properties and polyelectrolyte on electrospinning of ultrafine poly(ethylene oxide) fibers. Polymer, 2004. 45(9): 2959-2966. Chen, G., Guo, J., Nie, J. and Ma, G., Preparation, characterization, and application of PEO/HA core shell nanofibers based on electric field induced phase separation during electrospinning. Polymer, 2016. 83: 12-19. Dilamian, M., Montazer, M. and Masoumi, J., Antimicrobial electrospun membranes of chitosan/poly(ethylene oxide) incorporating poly(hexamethylene biguanide) hydrochloride. Carbohydrate Polymers, 2013. 94(1): 364- 371. Knaul, J.Z., Hudson, S.M. and Creber, K.A.M., Crosslinking of Chitosan Fibers with Dialdehydes: Proposal of a New Reaction Mechanism. Journal of Polymer Science: Part B: Polymer Physics, 1999. 37(11): 1079-1094. Bigi, A., Cojazzi, G., Panzavolta, S., Rubini, K. and Roveri, N., Mechanical and thermal properties of gelatin films at different degrees of glutaraldehyde crosslinking. Biomaterials, 2001. 22(8): 763-768. Hu, H., Xin, J.H., Hu, H., Chan, A. and He, L., Glutaraldehyde-chitosan and poly (vinyl alcohol) blends, and fluorescence of their nano-silica composite films. Carbohydrate Polymers, 2013. 91(1): 305- 313. Hennink,W.E., Nostrum, C.F.V., Novel crosslinking methods to design hydrogels. Advanced Drug Delivery Reviews, 2002. 54(1): 13-36. Baldan, A., Adhesively-bonded joints and repairs in metallic alloys, polymers and composite materials: Adhesives, adhesion theories and surface pretreatment. Journal of Materials Science, 2004. 39(1): 1-49. 蔡曉雯、鄭雅云,生物組織黏著劑開發與應用,長庚大學生化與生醫工程研究所,http://enews.cgu.edu.tw/files/15-1068-45403,c7774-1.php?Lang=zh-tw. Daniele, E. and Dissanaike, S., BioGlue for traumatic liver laceration. International Journal of Surgery Case Reports, 2016. 23: 33-35. Kim, H.J., Hwang, B.H., Lim, S., Choi, B.-H., Kang, S.H. and Cha, H.J., Mussel adhesion-employed water-immiscible fluid bioadhesive for urinary fistula sealing. Biomaterials, 2015. 72: 104-111. Eslah, F., Jonoobi, M., Faezipour, M., Afsharpour, M. and Enayati, A.A., Preparation and development of a chemically modified bio-adhesive derived from soybean flour protein. International Journal of Adhesion & Adhesives, 2016. 71: 48-54. Kawai, N., Suzuki, S., Naito, H., Kushibe, K., Tojo, T., Ikada, Y. and Taniguchi, T., Sealing Effect of Cross-Linked Gelatin Glue in the Rat Lung Air Leak Model. The Annals of Thoracic Surgery, 2016. 102: 282-286. 陳建志, 以電紡絲法製備聚己內酯/動物明膠/透明質酸混合奈米纖維及其應用. 國立台灣大學化學工程研究所碩士論文, 2014. 郭婷芸、林哲民、黃欣儀、謝學真,以物理方法改進幾丁聚醣/動物明膠/聚氧化乙烯電紡纖維膜之機械性質,2017年幾丁質幾丁聚醣暨生物材料研討會論文集,第67-70頁,新北市,6月23日,2017。 張雲皓, 製備以生物黏著劑黏合之非對稱雙層膜材及其特性探討. 國立台灣大學化學工程研究所碩士論文, 2017. ATSM International, Standard Test Method for Peel Resistance of Adhesives (T-Peel Test, D1876-01), 2001. Miller, J. D., Veeramasuneni, S., Drelich, J., Yalamanchili, M.R. and Yamauchi, G., Effect of roughness as determined by atomic force microscopy on the wetting properties of PTFE thin films. Polymer Engineering and Science, 1996. 36(14): 1849-1855. Yang, C., Tartaglino, U. and Persson, B. N. J., Influence of Surface Roughness on Superhydrophobicity. Physical Review Letters, 2006. 97: 116103. Zhang, X.-Y., Ouyang, Z., Schulze, R., Keller, T.F., Jandt, K.D. and Su, Z.-Q., Pathway mediated microstructures and phase morphologies of asymmetric double crystalline co-oligomers. RSC Advances, 2014. 4(16): 7900-7910. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/77958 | - |
dc.description.abstract | 奈米纖維的結構類似於細胞外間質(ECM),具有良好的質傳效率,因此在組織工程相關的領域具有高度潛力。幾丁聚醣(C)和明膠(G)擁有高生物相容性和親水性,聚氧化乙烯(P)可以改善電紡纖維的型態,幾丁聚醣-明膠-聚氧化乙烯溶液可被電紡成三成分的C-G-P膜。為了改善纖維膜的機械性質,聚己內酯(PCL)溶液可被電紡成單成分的PCL膜。本研究擬結合上述材料的優點,使用雙針電紡的技術來製備雙層奈米纖維膜(亦即PCL/C-G-P膜),再以戊二醛(GA)蒸氣進行交聯。本研究發現在雙層奈米纖維膜上難以電紡第三層纖維膜,為了製備出多層奈米纖維膜並增強纖維膜之間的黏著強度,本研究使用由明膠和戊二醛混合而成的水膠當作生物黏著劑(bioadhesive)。
本研究以掃瞄式電子顯微鏡(SEM)觀察奈米纖維的結構,而透過機械性質的測定可以分析奈米纖維膜經由不同交聯時間處理後,強度和延展性的變化,除了研究雙針電紡的製程參數之外,本研究也觀察到隨著交聯時間的增加,奈米纖維膜的強度會上升,但延展性會下降。由崩解性實驗可以發現當交聯達到一定程度後,奈米纖維的型態和重量就可以被維持,從以上實驗得知將奈米纖維膜交聯兩小時為最佳製備條件。由接觸角儀可以驗證PCL膜為疏水性膜材而C-G-P膜為親水性膜材,由傅立葉紅外線光譜儀(FT-IR)可以驗證製備出的支架確實為雙層結構。在熱性質的測定中,由熱重示差同步掃描分析儀(TGA)和差式掃描熱量分析(DSC)可以得知膜材的熱性質。在細胞相容性測試方面,纖維母細胞(WS1)和間葉幹細胞(KP-hMSC)分別被培養在雙層奈米纖維膜(PCL/C-G-P膜)的兩側,由實驗結果觀察到不論是疏水的PCL膜還是親水的C-G-P膜皆具有良好的細胞相容性,但細胞培養在C-G-P膜上時具有較快的增殖速率,故應可嘗試將本研究製備出的雙層奈米纖維膜應用於引導骨再生(GBR)手術的屏障膜(barrier membrane),因為疏水的PCL膜可以使不該往骨缺陷處生長的細胞被屏蔽在外面,同時親水的C-G-P膜擁有促進細胞貼附和增殖的功能。 | zh_TW |
dc.description.abstract | Nanofibers are structurally similar to extracellular matrix (ECM), possessing good mass transfer efficiency, and thus have great potential in the tissue engineering-related applications. Chitosan (C) and gelatin (G) have high biocompatibility and hydrophilicity properties; poly(ethylene oxide) (P) can improve the quality of fibers. A chitosan-gelatin-PEO solution can be fabricated into a three-component C-G-P nanofiber membrane. To improve the mechanical strength of membrane, polycaprolactone (PCL) solution can be fabricated into a one-component PCL nanofiber membrane. The purpose of this research is to develop scaffolds that possess both mechanical strength and biocompatibility, namely PCL/C-G-P bilayer electrospun nanofiber membranes which were prepared by dual-needle electrospinning technique and crosslinked by glutaraldehyde (GA) vapor. It was difficult to add the third layer to the bilayer scaffold. In order to develop mutli-layer electrospun nanofiber scaffolds and increase the bonding strength between layers, gelatin/glutaraldehyde solution was used as a bioadhesive to bond the scaffold.
In this study, SEM was used to observe the morphology of the PCL/C-G-P nanofiber membranes. The optimal operating conditions for dual-needle electrospinning such as the process parameters were analyzed and the results of mechanical property test showed that the tensile strength of the PCL/C-G-P nanofiber membranes was increased but the elongation at break was decreased after crosslinking. The results of membrane stability in aqueous solution showed that the morphology ant weight of membranes could be maintained after optimal crosslinking which was 2 hours in GA vapor. The results of water contact angle measurements showed that PCL membranes were hydrophobic and C-G-P membranes were hydrophilic. The bilayer structure of the PCL/C-G-P membranes were verified by FT-IR analysis. The results of TGA and DSC curves showed the thermal properties of membranes. In cytocompatibility tests, fibroblasts (WS1) and mesenchymal stem cells (KP-hMSC) were cultured on the PCL/C-G-P membranes. Both PCL membranes and C-G-P membranes showed excellent cytocompatibility, but cells cultured on C-G-P membranes exhibited higher proliferation rate than on PCL membranes. The PCL/C-G-P bilayer nanofiber membranes could be utilized as barrier membranes for guided bone regeneration (GBR), because the PCL membranes could block the migration of connective tissues due to its hydrophobic property while the C-G-P membranes could promote the attachment and proliferation of cells. | en |
dc.description.provenance | Made available in DSpace on 2021-07-11T14:38:18Z (GMT). No. of bitstreams: 1 ntu-106-R04524046-1.pdf: 5268031 bytes, checksum: dc8c4a71428a4c2bea1961c35a4f6b7b (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 目 錄
誌 謝 i 摘 要 iii Abstract v 目 錄 vii 圖目錄 xi 表目錄 xv 符號與縮寫說明 xvii 中英名詞對照表 xix 第一章 緒論 1 1.1 研究背景與動機 1 1.2 實驗架構與流程 3 第二章 文獻回顧 5 2.1 引導骨再生手術 5 2.2 奈米纖維材料在生醫的應用及優缺點 6 2.3 靜電紡絲法 7 2.3.1 靜電紡絲發展及原理 7 2.3.2 靜電紡絲影響因素 9 2.3.3 靜電紡絲裝置分類 13 2.3.4 靜電紡絲材料種類 18 2.4 生醫材料 19 2.4.1 聚己內脂 19 2.4.2 幾丁聚醣 20 2.4.3 動物明膠 21 2.4.4 聚氧化乙烯 22 2.5 交聯劑 23 2.6 黏著劑與黏著原理 24 2.6.1 黏著原理 24 2.6.2 生物黏著劑 26 第三章 實驗藥品、儀器與方法 29 3.1 實驗材料 29 3.2 實驗儀器 30 3.3 實驗方法 32 3.3.1 溶液配製 32 3.3.2 電紡纖維膜製備 33 3.3.3 電紡纖維膜交聯方法 35 3.3.4 生物黏著劑製備 36 3.3.5 雙層纖維膜製備 37 3.3.6 單層/雙層纖維膜性質分析 38 3.3.7 細胞相容性測定 47 第四章 實驗結果與討論 51 4.1 雙層電紡纖維膜(無生物黏著劑) 51 4.1.1 製程參數對纖維型態(SEM)及機械強度的影響 51 4.1.2 電紡順序對雙層纖維膜組成比例的影響 56 4.2 雙層電紡纖維膜(使用生物黏著劑加強黏著效果) 58 4.2.1 生物黏著劑成膠時間 58 4.2.2 黏著強度測定 59 4.3 機械性質測定 64 4.4 崩解性測定 70 4.5 材料表面性質測定 77 4.5.1 水接觸角 77 4.5.2 FT-IR 80 4.6 熱性質測定 81 4.6.1 TGA 81 4.6.2 DSC 83 4.7 細胞相容性測定 88 4.7.1 細胞蛋白質總量測定(細胞增殖測定) 88 4.7.2 MTT 測定(細胞活性測定) 91 第五章 結論與未來研究方向 95 5.1 結論 95 5.2 未來研究方向 97 參考文獻 99 | |
dc.language.iso | zh-TW | |
dc.title | 聚己內酯及幾丁聚醣-明膠-聚氧化乙烯雙層電紡奈米纖維膜之製備及其特性探討 | zh_TW |
dc.title | Fabrication and Characterization of Polycaprolactone and Chitosan-Gelatin-Poly(ethylene oxide) Bilayer Electrospun Nanofiber Membranes | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 何明樺(Ming-Hua Ho),謝子陽(Tzu-Yang Hsieh) | |
dc.subject.keyword | 靜電紡絲法,聚己內酯,幾丁聚醣,明膠,雙層膜,生物黏著劑, | zh_TW |
dc.subject.keyword | electrospinning,polycaprolactone,chitosan,gelatin,bilayer,bioadhesive, | en |
dc.relation.page | 105 | |
dc.identifier.doi | 10.6342/NTU201700893 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-07-27 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
顯示於系所單位: | 化學工程學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-106-R04524046-1.pdf 目前未授權公開取用 | 5.14 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。