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標題: | 乙烯反應因子ERF19參與植物模式誘發免疫反應之功能性研究 Functional Studies of Ethylene Response Factor 19 in Plant Pattern-Triggered Immunity |
作者: | Pin-Yao Huang 黃品堯 |
指導教授: | 金洛仁 |
關鍵字: | 阿拉伯芥,乙烯反應因子,模式誘發免疫反應, Arabidopsis thaliana,ethylene response factor,pattern-triggered immunity, |
出版年 : | 2017 |
學位: | 博士 |
摘要: | 植物病原菌的興起加上氣候變遷威脅著全球的糧食安全,透過生物工程改良的作物,是解決植物病害的可靠方案,而抗病作物的發展是來自我們對於植物與病原菌交互作用的科學認知。目前研究發現,植物透過多元和有效的防禦機制來抵禦病原菌的入侵,植物防禦的啟動是利用細胞膜上的模式辨識受體(pattern recognition receptors, PRR),來辨識微生物或病原菌上的組成分子,亦稱為微生物或病原菌相關分子模式(microbe- or pathogen-associated molecular patterns, MAMPs/PAMPs),MAMP是演化上具有保守性且對於微生物生存不可或缺的重要分子,如細菌的鞭毛蛋白或構成真菌細胞壁的幾丁質。植物辨識MAMP之後,會透過轉錄因子(transcription factors, TFs)來重新編程植物細胞轉錄反應,進而啟動模式誘發免疫反應(pattern-triggered immunity, PTI)來抵禦入侵的病原菌。雖然PTI的分子機制的研究逐年增加,但是PTI運用於農業病害改良的研究仍在起步階段。除此之外,為了更了解PTI的調控,我們需要針對PTI的轉錄控制有更深入的認知。我的博士論文即針對這兩項議題進行研究。我的研究結果證實可以利用阿拉伯芥(Arabidopsis thaliana)的PTI正向調控基因,來廣幅提升煙草(Nicotiana benthamiana)對病原菌的抗性,該研究成果已發表於Plant Journal期刊(附錄1)。我也撰寫了兩篇文獻探討,論述利用PTI來增進作物抗病的可行性(附錄2),與分析探究乙烯反應因子(ethylene response factors, ERFs)如何參與植物免疫反應(附錄3)。我的博士論文正文著重於我對乙烯反應因子ERF19參與調控PTI的功能性研究。 Emerging plant pathogens combined with climate change threaten global food security. Bio-engineered crops provide a promising solution to combat the uprising of plant diseases. The development of disease resistant crops relies on our scientific understanding of the fundamental nature of plant-pathogen interactions. The initiation of plant defense depends on the recognition of molecules which are derived from the invading pathogens and also known as microbe- or pathogen-associated molecular patterns (MAMPs/PAMPs) by pattern recognition receptors (PRRs) localized at the plant plasma membrane. MAMPs such as bacterial flagellin and chitin in the fungal cell wall are evolutionarily conserved across a certain class of microbes and are essential for the microbial lifestyle. After MAMP perception, plant transcription factors (TFs) reprogram cellular transcription toward activation of the pattern-triggered immunity (PTI) responses, to block invading pathogens. In spite of increasing reports describing different aspects of PTI molecular mechanisms, application of PTI to improve agriculture is still in its infancy. Notably, to increase our understanding of the complex regulation of PTI, we need deeper insights into the transcriptional control of PTI. Therefore, my PhD study centers on tackling these two major questions. I exemplified the use of a positive regulator of PTI from Arabidopsis thaliana to promote broad-spectrum resistance in Nicotiana benthamiana, and this work was published in Plant Journal (Appendix 1), a well-renowned journal in plant science. I also wrote two literature reviews, which point out the potential use of PTI as a tool to enhance crop resistance (Appendix 2) and summarize the molecular functions of ethylene response factors (ERFs) in plant immunity (Appendix 3). The main content of my PhD dissertation focuses on my latest research on ERF19, notably its role in the modulation of the PTI response. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/77746 |
DOI: | 10.6342/NTU201702523 |
全文授權: | 未授權 |
顯示於系所單位: | 植物科學研究所 |
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