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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 戴子安 | |
| dc.contributor.author | Po-An Chou | en |
| dc.contributor.author | 周柏安 | zh_TW |
| dc.date.accessioned | 2021-07-10T22:17:58Z | - |
| dc.date.available | 2021-07-10T22:17:58Z | - |
| dc.date.copyright | 2017-08-20 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-08-11 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/77722 | - |
| dc.description.abstract | 本研究目的為開發一可利用積層製造方式列印人工角膜的生物相容性水膠。本研究使用Poloxamer 407 (P407)高分子做為水膠材料，P407為一具有熱感性質之材料，高分子溶液當達到低臨界溶解溫度時會由液態轉變為凝膠態。此外，P407末端官能基羥基可經尾端改質反應而形成具有光感性質之丙烯酸官能基高分子，此材料便可以應用於3D列印以製造客製化人工角膜。
P407水膠具有良好的生物相容性及極佳的透光度，然而水膠本身的機械強度偏弱，會使得臨床手術難以進行。為了增強水膠的機械性質，本研究用高分子互穿網路的方式，分別以2-hydroxyethyl methacrylate (HEMA) 和acrylic acid (AA) 單體和P407水膠形成雙層網路結構。具有生物相容性、可長期使用之人工角膜，角膜上皮細胞貼附於水膠並在其上成長繁殖為一重要的要求。P407和PAA所形成之互穿網路水膠具有排斥蛋白質及細胞的特性，為了要使細胞能夠在上面附著及生長，本研究以EDC/NHS的化學鍵結方式，使膠原蛋白 (collagen)可以固定在水膠上，改善上皮細胞貼附及成長情況。P407和PHEMA所形成之互穿網路水膠則以膠原蛋白吸附的方式改善細胞貼附狀況。研究結果顯示，此IPN水膠具有極佳的透光度、含水率、適當的機械強度及良好的細胞貼附成長情況，為一具有潛力的人工角膜材料。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-07-10T22:17:58Z (GMT). No. of bitstreams: 1 ntu-106-R04524069-1.pdf: 2903472 bytes, checksum: 845538498c8d6f0ae410e37b70a22f28 (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 致謝 I
中文摘要 II Abstract III Contents V List of Figures IX List of Tables XIV Chapter 1. Introduction 1 Chapter 2. Literature Review 5 2-1 The Human Cornea 5 2-1-1 Corneal epithelium 6 2-1-2 Bowman’s layer 7 2-1-3 Corneal stroma 8 2-1-4 Corneal endothelium 9 2-1-5 Descemet’s membrane 10 2-2 Keratoprostheses 11 2-2-1 Boston KPro 12 2-2-2 AlphaCor 14 2-3 Hydrogel 16 2-3-1 Poloxamer 407 16 2-4 Additive Manufacturing 18 2-4-1 Fused deposition modeling (FDM) 20 2-4-2 Stereolithography (SLA) 21 2-4-3 Inkjet 3D printing 22 Chapter 3. Experimental Section 23 3-1 Materials and Equipment 23 3-2 Synthesis of Diacrylate-Terminated Macromers by Nucleophilic Acyl Substitution 26 3-2-1 Synthesis of Poloxamer 407 Diacrylate (P407DA) 26 3-3 Preparation of P407 Aqueous Solutions 28 3-4 Hydrogels Preparation 29 3-4-1 Synthesis of Interpenetrating Network Hydrogel from End-linked Poloxamer 407 (P407) and Crosslinked Poly 2-hydroxyethyl methacrylate (PHEMA) 29 3-4-2 Synthesis of Interpenetrating Network Hydrogel from End-linked Poloxamer 407 (P407) and Crosslinked Poly(acrylic acid) (PAA) 32 3-5 Surface Modification of the IPN Hydrogels 35 3-5-1 P407/PHEMA IPN hydrogel 35 3-5-2 P407/PAA IPN hydrogel 38 3-6 Characterization of Macromers and Hydrogels 41 3-6-1 Thermorheologic Properties Measurements 41 3-6-2 1H Nuclear Magnetic Resonance (1H NMR) Characterization of Macromers 41 3-6-3 Optical Characteristics 41 3-6-4 Tensile Strength Tests 42 3-6-5 Swelling Studies 43 3-6-6 In Vitro Assessments 44 3-6-7 Subcutaneous Implantation Studies 47 Chapter 4. Result and Discussion 49 4-1 Thermorheologic Properties of Precusor Solution For 3D printing 49 4-2 1H Nuclear Magnetic Resonance (1H NMR) Spectrum of P407DA 52 4-3 Optical Characteristics 54 4-4 Swelling Studies 59 4-5 Tensile Strength Tests 63 4-6 Surface Modification and Collagen Immobilization 70 4-6-1 P407/PHEMA IPN hydrogel 71 4-6-2 P407/PAA IPN hydrogel 74 4-7 In Vitro Assessments 79 4-8 Subcutaneous Implantation Studies 88 Chapter 5. Conclusion 92 Reference 94 | |
| dc.language.iso | en | |
| dc.subject | 積層製造 | zh_TW |
| dc.subject | 高分子互穿網路 | zh_TW |
| dc.subject | 水膠 | zh_TW |
| dc.subject | 人工角膜 | zh_TW |
| dc.subject | additive manufacturing | en |
| dc.subject | hydrogel | en |
| dc.subject | interpenetrating polymer network (IPN) | en |
| dc.subject | Poloxamer 407 (P407) | en |
| dc.subject | artificial cornea | en |
| dc.title | 積層製造人工角膜之生物相容水膠設計與開發 | zh_TW |
| dc.title | Design and Fabrication of Biocompatible Artificial Corneal Hydrogel by Additive Manufacturing | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 謝之真,施博仁,王一中,王勝仕 | |
| dc.subject.keyword | 人工角膜,積層製造,水膠,高分子互穿網路, | zh_TW |
| dc.subject.keyword | hydrogel,artificial cornea,interpenetrating polymer network (IPN),Poloxamer 407 (P407),additive manufacturing, | en |
| dc.relation.page | 97 | |
| dc.identifier.doi | 10.6342/NTU201703020 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2017-08-11 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
| Appears in Collections: | 化學工程學系 | |
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| ntu-106-R04524069-1.pdf Restricted Access | 2.84 MB | Adobe PDF |
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