攜帶式微流體疾病檢測裝置開發

Abstract

本研究開發一微流體系統，其結合紙張與塑膠材料本身優點，(polymer/paper hybrid enzyme-linked immunosorbent assay，hELISA)。研究係藉由塑膠微流道系統只需微量樣品與試劑反應之特性，以及其高流體變因可控制性，與可事先儲存試劑於流道中來減少使用者操作步驟的優點，並結合紙張提供將抗體長效存放，以及其本身為白色的特性，因此進行免疫反應呈色結果相較於小尺寸透明塑膠微流道能更清楚呈現酵素-受質(enzyme-substrate)反應所產生可見光範圍顏色變化。此外，吾人也使用3D列印技術，製造出可重複使用之攜帶式手動液體幫浦，達到不需要外加電力便可手動驅動液體於微流體晶片內流動之目的。最終研究也利用影像分析技術進行影像紅綠藍(red-green-blue，RGB)三原色數值分析，經過邏輯運算後，可由智慧型手機獲得檢測結果，達到快速疾病檢測分析系統目標。研究結果成功進行人類免疫球蛋白檢測，在16分鐘內時間內，獲得0.03 ng/mL之偵測極限。吾人預期此項檢測系統可輕易置換使用不同抗體，進行其他多樣化疾病檢測，達到於資源匱乏區域疾病篩選與病程監控之目的。

We develop a microfluidic system which integrates the advantages of paper and plastic materials to propose a new hybrid microfluidic immunoassay platform. Polymeric microfluidic system possesses advantages such as small sample volume, high fluid controllability, and reagent pre-filling. In this study, we combine the paper device to achieve long-term storage of antibodies and visually much clear colorimetric sensing results than in micro size transparent plastic microchannels. On the other hand, we use 3D printing approaches to fabricate a reusable, portable, and hand-powered liquid pump to drive liquid in microchannels without electricity. In addition, we use image processing to analyze the RGB value to quantify detection results. After logic calculation, the results can be obtained directly by a smart phone, and the rapid disease detection system can be accomplished. As a result, the detection limit of 0.03 ng/mL can be achieved in 20 minutes for the human Immunoglobulin G (IgG) tests. We expect different detection targets can be easily carried out easily by replacing receptors for various disease, and the system possesses the potential for disease screening and monitoring in resource-limited regions.