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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 徐善慧 | zh_TW |
| dc.contributor.advisor | Shan-hui Hsu | en |
| dc.contributor.author | 林子為 | zh_TW |
| dc.contributor.author | Tzu-Wei Lin | en |
| dc.date.accessioned | 2021-07-10T21:51:35Z | - |
| dc.date.available | 2024-08-19 | - |
| dc.date.copyright | 2019-08-26 | - |
| dc.date.issued | 2019 | - |
| dc.date.submitted | 2002-01-01 | - |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/77222 | - |
| dc.description.abstract | 水凝膠由於其高保水性和柔軟特性而廣泛用於組織工程中。開發具有可調節降解速率，適當環境響應性和可注射性的水凝膠仍然是一個挑戰。在這項研究中，可生物降解的雙官能聚氨酯（DFPU）奈米粒子分散體是由一種涉及使用乙二醛的水性製程合成的。這種DFPU 用於交聯乙二醇殼聚醣（CS）。在室溫下DFPU 和CS鍵結形成席夫鹼，成功製備出自癒合水凝膠。此外，在-20 ℃冷凍後產生冷凍凝膠。由於席夫鹼的性質，發現這些凝膠對pH 值和含胺分子敏感。此外，降解速率可以通過合成DFPU 之寡聚二醇類型進行調整。經由流變學測試證實了優異的自癒性能（損傷後恢復約100％）。自癒合凝膠和冷凍凝膠均可注射（分別通過26 號針和18 號針），並顯示出良好的細胞增殖。14 天大鼠植入顯示冷凍凝膠的低免疫應答。功能化的可生物降解聚胺酯奈米粒子為一交聯劑的新平台，可與含胺基生物大分子（例如殼聚醣）進行動態席夫反應，其有潛力產生應用於生物醫學的各種自癒合水凝膠和冷凍凝膠。 | zh_TW |
| dc.description.abstract | Hydrogels are widely used in tissue engineering owing to their high water retention and soft characteristics. It remains a challenge to develop hydrogels with tunable degradation rates, proper environmental responsiveness, and injectability. In this study, biodegradable difunctional polyurethane (DFPU) nanoparticle dispersions were synthesized from an eco-friendly waterborne process involving the use of glyoxal. Such DFPU was used to crosslink chitosan (CS). The Schiff base linkage between DFPU and CS successfully produced self-healing hydrogels at room temperature. Moreover, cryogels were generated after being frozen at -20 °C. These gels were found to be sensitive to pH values and amine-containing molecules owing to the property of Schiff bases. Besides, the degradation rates could be adjusted by the types of the component oligodiols in DFPU. Rheological evaluation verified the excellent self-healing properties (~100% recovery after damage). Both the self-healing gels and cryogels were injectable (through 26-gauge and 18-gauge needles, respectively) and demonstrated good cell proliferation. Rat implantation at 14 days showed low immune responses of cryogels. The functionalized biodegradable polyurethane nanoparticles represent a new platform of crosslinkers for biomacromolecules such as chitosan through dynamic Schiff reaction that may give rise to a wide varieties of self-healing gels and cryogels for biomedical applications. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-10T21:51:35Z (GMT). No. of bitstreams: 1 ntu-108-R06549020-1.pdf: 3472640 bytes, checksum: 9747191ef0899e1506380960c6cbde42 (MD5) Previous issue date: 2019 | en |
| dc.description.tableofcontents | 目錄
口試委員會審定書 ........................................................................................................... I 致謝 .................................................................................................................................. II 摘要 ................................................................................................................................ III Abstract ........................................................................................................................... IV 目錄 ................................................................................................................................ VI 圖目錄 ............................................................................................................................ XI 表目錄 .......................................................................................................................... XIII 第一章 文獻回顧 ............................................................................................................ 1 1.1. 水凝膠 .............................................................................................................. 1 1.2. 環境響應型水凝膠 .......................................................................................... 1 1.3. 可注射水凝膠 .................................................................................................. 1 1.4. 自癒合水凝膠 .................................................................................................. 2 1.5. 冷凍凝膠 .......................................................................................................... 2 1.6. 聚胺酯 .............................................................................................................. 3 1.7. 交聯劑 .............................................................................................................. 4 1.8. 殼聚醣 .............................................................................................................. 4 1.9. 席夫鹼 .............................................................................................................. 5 1.10. 研究目的 ........................................................................................................ 5 第二章 研究方法 ............................................................................................................ 6 2.1. 研究架構 .......................................................................................................... 6 2.2. 水性聚胺酯交聯劑之合成 .............................................................................. 8 2.3. 表徵水性聚胺酯交聯劑 .................................................................................. 9 2.3.1. 奈米粒徑分析儀分析 ........................................................................... 9 2.3.2. 膠體滲透層析儀分析 ......................................................................... 10 2.3.3. 穿透式電子顯微鏡分析 ..................................................................... 10 2.3.4. 小角度X 光散射分析 ........................................................................ 10 2.3.5. 衰减全反射傅立葉紅外光譜儀分析 ................................................. 10 2.3.6. X 光繞射儀分析 .................................................................................. 10 2.4. 製備CS-PU 自癒合水凝膠與冷凍凝膠 ....................................................... 11 2.5. 表徵CS-PU 自癒合水凝膠 ........................................................................... 12 2.5.1. 巨觀自癒合測試 ................................................................................. 12 2.5.2. 流變分析 ............................................................................................. 12 2.5.3. 熱性能分析 ......................................................................................... 12 2.6. 表徵CS-PU 冷凍凝膠 ................................................................................... 13 2.6.1. 動態機械分析儀分析 ......................................................................... 13 2.6.2. 孔隙率分析 ......................................................................................... 13 2.6.3. 溶脹度分析 ......................................................................................... 13 2.6.4. 掃描式電子顯微鏡分析 ..................................................................... 13 2.6.5. 注射性測試 ......................................................................................... 14 2.7. CS-PU 自癒合水凝膠與冷凍凝膠之環境響應性 ......................................... 14 2.8. CS-PU、CS-PU'自癒合水凝膠與冷凍凝膠之體外降解 .............................. 14 2.9. 交聯度分析 .................................................................................................... 14 2.10. 細胞實驗 ...................................................................................................... 15 2.10.1. 細胞培養 ........................................................................................... 15 2.10.2 細胞於CS-PU 自癒合水凝膠與冷凍凝膠中之存活率與增殖 ....... 15 2.11. 大鼠皮下實驗 .............................................................................................. 16 2.11.1.大鼠皮下材料植入 ............................................................................. 16 2.11.2. 組織切片 ........................................................................................... 16 2.11.3. 蘇木精-伊紅染色 .............................................................................. 16 2.11.4. 免疫螢光染色 ................................................................................... 17 2.12. 統計學分析 .................................................................................................. 17 第三章 實驗結果 .......................................................................................................... 18 3.1. 表徵水性聚胺酯交聯劑 ................................................................................ 18 3.1.1. 奈米粒徑分析儀與膠體滲透層析儀分析 ......................................... 18 3.1.2. 穿透式電子顯微鏡分析 ..................................................................... 18 3.1.3. 小角度X 光散射分析 ........................................................................ 18 3.1.4. 衰减全反射傅立葉紅外光譜儀分析 ................................................. 19 3.1.5. X 光繞射儀分析 .................................................................................. 19 3.2. 優化CS-PU 自癒合水凝膠之組成比例 ....................................................... 19 3.3. 表徵CS-PU 自癒合水凝膠 ........................................................................... 20 3.3.1. 巨觀自癒合測試 ................................................................................. 20 3.3.2. 流變性能測試 ..................................................................................... 20 3.3.3. 可注射性測試 ..................................................................................... 20 3.4. 表徵CS-PU 冷凍凝膠 ................................................................................... 21 3.4.1. 基本物理性質測試(可壓縮性、溶脹比、孔隙度、機械性質)....... 21 3.4.2. 溶脹速率分析 ..................................................................................... 21 3.4.3. 掃描式電子顯微鏡分析 ..................................................................... 21 3.4.4. 熱穩定性分析 ..................................................................................... 21 3.4.5. 形狀回復、可注射性質 ..................................................................... 22 3.5. CS-PU 自癒合水凝膠與冷凍凝膠之響應性分析 ......................................... 22 3.6. CS-PU、CS-PU'自癒合水凝膠與冷凍凝膠之體外降解 .............................. 22 3.7. CS-PU 自癒合水凝膠與冷凍凝膠含細胞之存活率與增殖 ......................... 23 3.8. 大鼠皮下植入 ................................................................................................ 23 3.8.1. 蘇木精-伊紅染色分析 ........................................................................ 23 3.8.2. 免疫染色分析 ..................................................................................... 23 第四章 討論 .................................................................................................................. 24 4.1. 水性聚胺酯交聯劑基本性質分析 ................................................................ 24 4.2. 優化CS-PU 自癒合水凝膠組成比例 ........................................................... 24 4.3. CS-PU 自癒合水凝膠性質分析 ..................................................................... 24 4.4. CS-PU 冷凍凝膠性質分析 ............................................................................. 25 4.5. CS-PU 自癒合水凝膠與冷凍凝膠之響應性分析 ......................................... 26 4.6. CS-PU、CS-PU'自癒合水凝膠與冷凍凝膠之體外降解調控分析 .............. 26 4.7. CS-PU 自癒合水凝膠與冷凍凝膠含細胞之存活率分析 ............................. 26 4.8. 大鼠皮下實驗分析 ........................................................................................ 27 4.9. 未來展望 ........................................................................................................ 27 第五章 結論 .................................................................................................................. 28 參考文獻 ........................................................................................................................ 29 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 聚胺酯奈米粒子 | zh_TW |
| dc.subject | 交聯劑 | zh_TW |
| dc.subject | 殼聚醣 | zh_TW |
| dc.subject | 自癒合水凝膠 | zh_TW |
| dc.subject | 冷凍凝膠 | zh_TW |
| dc.subject | chitosan | en |
| dc.subject | self-healing hydrogel | en |
| dc.subject | polyurethane nanoparticle | en |
| dc.subject | cryogel | en |
| dc.subject | crosslinker | en |
| dc.title | 生物可降解聚胺酯奈米粒子交聯殼聚醣之自癒合水凝膠與冷凍凝膠 | zh_TW |
| dc.title | Self-healing hydrogels and cryogels from biodegradable polyurethane nanoparticle crosslinked chitosan | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 107-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 侯詠德;張書瑋;黃翠薇 | zh_TW |
| dc.contributor.oralexamcommittee | Yung-Te Hou;Shu-Wei Chang;Chui-Wei Wong | en |
| dc.subject.keyword | 聚胺酯奈米粒子,交聯劑,殼聚醣,自癒合水凝膠,冷凍凝膠, | zh_TW |
| dc.subject.keyword | polyurethane nanoparticle,chitosan,crosslinker,self-healing hydrogel,cryogel, | en |
| dc.relation.page | 59 | - |
| dc.identifier.doi | 10.6342/NTU201903716 | - |
| dc.rights.note | 未授權 | - |
| dc.date.accepted | 2019-08-15 | - |
| dc.contributor.author-college | 工學院 | - |
| dc.contributor.author-dept | 高分子科學與工程學研究所 | - |
| 顯示於系所單位： | 高分子科學與工程學研究所 | |
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