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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 顏瑞泓(Jui-Hung Yen) | |
| dc.contributor.author | Chia-Chun Yu | en |
| dc.contributor.author | 余佳純 | zh_TW |
| dc.date.accessioned | 2021-07-10T21:40:01Z | - |
| dc.date.available | 2021-07-10T21:40:01Z | - |
| dc.date.copyright | 2020-09-24 | |
| dc.date.issued | 2020 | |
| dc.date.submitted | 2020-08-11 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76903 | - |
| dc.description.abstract | 生物型除草劑的優點為兼顧環境友善、使用者安全以及消費者健康，更具有減少使用人工合成化學藥劑的優勢。本研究利用自水稻植物中篩選出之內生細菌 Bacillus aryabhattai MN1 具有可以產生高量吲哚-3-乙酸 (Indole-3-acetic Acid, IAA) 的特性，探討其於生物型除草的應用潛力。研究結果顯示 B. aryabhattai MN1 菌液上清液具有抑制雜草生長的能力，以高效液相層析儀分析以及生物鑑定法說明 B. aryabhattai MN1 所生產的上清液含有 IAA 且為影響植物之有效成分。另透過優化 B. aryabhattai MN1 的培養條件，使其能產生高量的IAA，產量最高可達 97.1 mg L-1。由萌前除草的藥效試驗中，以 IAA 基準濃度 10 mg L-1 施用量施用 MN1 上清液，即可抑制綠豆根長度的增長，並在施用 5 mL 60 mg L -1 (IAA) MN1 上清液後，阿拉伯芥和綠豆的發芽率相較於對照組分別下降了 33.3 和 60.0%；於萌後水耕除草藥效試驗中，發現施用上清液會對於阿拉伯芥並無顯著影響。而在添加展著劑之後，MN1 對於三週大小之大花咸豐草 (Bidens pilosa) 的殺草效果明顯上升，噴施 5 mL 40 mg L-1 (IAA) MN1 上清液會使葉片發生損傷、壞死、捲曲或掉落；此外，MN1上清液能選擇性地抑制大花咸豐草鮮重和乾重，對於水稻 (Oryza sativa L.) 則影響有限；而施用高濃度的 IAA MN1 上清液後，會使雜草發生細胞滲漏、葉綠素下降、丙二醛和抗壞血酸過氧化物酶濃度上升的生理現象。本研究結果顯示 B. aryabhattai MN1 具有可作為萌前和萌後雙子葉雜草生物型除草劑的潛力，達到控制田間雜草及環境友善的目的並減少化學合成藥劑的使用。 | zh_TW |
| dc.description.abstract | All environmental friendly, user safe, do health to consumers and synthetic chemical pesticide usage reduced are advantages of biological herbicides. In this study, we attempt to exploit endophytic bacteria Bacillus aryabhattai MN1, selected from rice plants, as a potential bio-herbicide due to its feature of high production of indole-3-acetic acid (IAA). Results indicate that supernatant produced by B. aryabhattai MN1 owns the ability to inhibit weeds growth. With high-performance liquid chromatography analysis and bioassay practice, supernatant produced by B. aryabhattai MN1 appears to contain IAA and act as active ingredients that affect plants growth. In addition, a maximum yield of 97.1 mg L-1 IAA production will be observed after optimizing the culture conditions of B. aryabhattai MN1. In the pre-emergence weeding efficacy test, MN1 supernatant is diluted based on IAA concentration as 10 mg L-1 of (IAA) MN1 treatment is capable of inhibiting green bean root length. Also, 5 mL of 60 mg L -1 (IAA) MN1 supernatant application may cause germination rates drop in Arabidopsis and green bean by 33.3 and 60.0%, in comparison to the control group, respectively. During the post-emergence hydroponic weeding efficacy test, it was found that the application of supernatant ends up no significant effect on Arabidopsis decline. However, with the spreading agent added, the effect of MN1 controlling three-weeks-old Bidens pilosa significantly increased. Spraying 5 mL of 40 mg L-1 (IAA) MN1 supernatant leads to different extent of leaf damage, necrosis, leaf curl or fall. Thus, MN1 supernatant can selectively inhibit fresh weight and dry weight of B. pilosa instead of rice (Oryza sativa L.). Besides, by applying high concentration of IAA MN1 supernatant, B. pilosa experience cell leakage, chlorophyll decline, increasing malondialdehyde and ascorbate peroxidase concentration. This study shows that B. aryabhattai MN1 possesses the potential to become a bio-herbicide on pre- and post- emergence dicotyledonous weeds control. With the development of bio-herbicide, we can achieve the goal of controlling weeds in the field and reduce the use of chemical synthetic herbicides. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-10T21:40:01Z (GMT). No. of bitstreams: 1 U0001-1008202018175000.pdf: 2894707 bytes, checksum: 18f4e88773572bf35cd70cc2d50754c3 (MD5) Previous issue date: 2020 | en |
| dc.description.tableofcontents | 壹、 前言 1 一、 全球農藥使用現況 1 二、 生物防治 3 三、 植物根圈細菌 7 四、 植物荷爾蒙與荷爾蒙類型除草劑的作用機制 10 五、 微生物生成吲哚-3-乙酸的機制 13 貳、 研究目的 15 參、 試驗架構 16 肆、 材料與方法 17 一、 菌株的保存與基本性質試驗 17 二、 初步評估 Bacillus aryabhattai MN1抑制植物生長的效果 24 三、 Bacillus aryabhattai MN1上清液有效成分鑑定 27 四、 最佳化 Bacillus aryabhattai MN1生產 IAA 條件 28 五、 Bacillus aryabhattai MN1萌前與萌後之除草藥效試驗 31 六、 Bacillus aryabhattai MN1 單子葉與雙子葉選擇性試驗 36 七、 Bacillus aryabhattai MN1 對植物之生理影響試驗 39 八、 統計分析 43 伍、 結果與討論 44 一、 Bacillus aryabhattai MN1 菌株基本性質 44 二、 初步評估Bacillus aryabhattai MN1 抑制植物生長的效果 46 三、 Bacillus aryabhattai MN1有效成分鑑定 49 四、 Bacillus aryabhattai MN1生產IAA條件試驗 51 五、 Bacillus aryabhattai MN1萌前與萌後的抑制效果試驗 59 （一） 阿拉伯芥對於 IAA 之感度差異 59 （二） Bacillus aryabhattai MN1上清液除草藥效試驗—萌前 62 （三） Bacillus aryabhattai MN1 上清液除草藥效試驗—萌後 67 （四） Bacillus aryabhattai MN1上清液對於大花咸豐草之除草藥效試驗 71 六、 Bacillus aryabhattai MN1 單子葉與雙子葉選擇性 74 七、 Bacillus aryabhattai MN1對植物之生理影響試驗 79 陸、 結論 89 柒、 參考資料 90 | |
| dc.language.iso | zh-TW | |
| dc.subject | 生物型除草劑 | zh_TW |
| dc.subject | 有害根圈細菌 | zh_TW |
| dc.subject | 吲哚-3-乙酸 | zh_TW |
| dc.subject | 生長抑制 | zh_TW |
| dc.subject | Bacillus aryabhattai MN1 | zh_TW |
| dc.subject | Indole-3-acetic acid | en |
| dc.subject | Bio-herbicide | en |
| dc.subject | Deleterious rhizobacteria | en |
| dc.subject | Growth inhibition | en |
| dc.subject | Bacillus aryabhattai MN1 | en |
| dc.title | Bacillus aryabhattai MN1 作為微生物除草劑之潛力研究 | zh_TW |
| dc.title | Research on Bacillus aryabhattai MN1 as a potential bio-herbicide | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 108-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林乃君(Nai-Chun Lin),黃文達(Wen-Dar HUANG),蔣永正(Yeong-Jene Chiang),陳玟瑾(Wen-Ching Chen) | |
| dc.subject.keyword | Bacillus aryabhattai MN1,生物型除草劑,吲哚-3-乙酸,有害根圈細菌,生長抑制, | zh_TW |
| dc.subject.keyword | Bacillus aryabhattai MN1,Bio-herbicide,Deleterious rhizobacteria,Growth inhibition,Indole-3-acetic acid, | en |
| dc.relation.page | 109 | |
| dc.identifier.doi | 10.6342/NTU202002856 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2020-08-12 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 植物醫學碩士學位學程 | zh_TW |
| 顯示於系所單位： | 植物醫學碩士學位學程 | |
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