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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76809完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 戴子安(Chi-An Dai) | |
| dc.contributor.author | CHONG-WEI XU | en |
| dc.contributor.author | 許崇瑋 | zh_TW |
| dc.date.accessioned | 2021-07-10T21:37:29Z | - |
| dc.date.available | 2021-07-10T21:37:29Z | - |
| dc.date.copyright | 2020-08-28 | |
| dc.date.issued | 2020 | |
| dc.date.submitted | 2020-08-17 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76809 | - |
| dc.description.abstract | 本研究開發一種可結合積層製造法(3D列印技術)的生物相容性高分子水膠配方作為仿生人工角膜材料。透過使用泊洛沙姆高分子(Poloxamer 407或P407)做為水膠主要材料。基於泊洛沙姆高分子具有低臨界溶液溫度(lower critical solution temperature, LCST)之特性，其在低溫時能於水互溶，當溶液溫度升高超過LCST時則由液態轉換成凝膠態，故可透過改變溫度從而調整泊洛沙姆水溶液黏度使其可應用於3D列印以客製化製造仿生人工角膜。本實驗透過改質泊洛沙姆高分子使其末端接上丙烯醯基團(P407DA)，讓高分子具有雙鍵，能在紫外光下激發起始劑產生自由基後進行自由基聚合反應形成一個三維的網路結構。利用小角度x光散射實驗(SAXS)，我們可以了解此溶液態/凝膠態的轉化，來自於三嵌段之P407DA高分子的自組裝特性的轉化所產生。 但由於泊洛沙姆水膠的機械強度較弱，不足以達到人工角膜的需求，本實驗透過加入明膠並使其在泊洛沙姆水膠裡交聯形成明膠網路使水膠成為互穿網路(interpenetrating network)系統增加水膠的機械強度。隨後便對水膠進行透光度、含水量、機械強度進行測試，根據水膠的物理試驗之實驗結果評估是否適合作為人眼角膜。隨後利用塗佈明膠包覆水膠增加生物相容性，根據實驗結果發現此水膠具有與人眼角膜相似的物性而且生物相容性方面也有改善，為一具有潛力的仿生人工角膜材料。最後，我利用實驗室製作之3D列印機，以加熱在30°C的溫度，以擠壓之方式將泊洛沙姆水膠/明膠之互穿網路列印成具備有弧度之角膜，並利用調節溫度以減小列印之紋路，因此驗證此類水膠可做為日後利用3D列印的方式製備客製化人工角膜之水膠材料。 | zh_TW |
| dc.description.abstract | In this study, a method to fabricate biocompatible soft artificial cornea hydrogels by using additive manufacturing method, capable of 3D printing corneas with customized biometric, was developed. In order to synergistically maintain the mechanical integrity for building dome-shaped cornea with a soft gel during layer printing and the flexibility of easy adjustment of gel solution formulation for biocompatibility, thermos-reversible hydrogels of Poloxamer triblock copolymer (P407), capable of undergoing a sol-gel transition to exhibit dramatic viscosity variation upon temperature changes, was used as the main material composition. This morphological transition, based on the self-assembling behavior of P407, was investigated by using small-angle x-ray scattering (SAXS) technique. Photosensitive P407 (P407DA) was first synthesized by modifying both of its hydroxyl chain ends with double bonds, preparing for crosslink reaction of the double bonds upon UV light exposure after 3D printing to fixate their final structure with free radical polymerization. For the purpose of further enhancing their mechanical property, transparent corneas with interpenetrating network (IPN) structure were made by mixing various amount of gelatin into P407DA as the final ingredient for 3D printing. The formulation was optimized so that similar sol-gel transition could be preserved to facilitate 3D printing. Afterwards, the IPN hydrogel was tested for its optical transparency, water content, and mechanical strength to compare with those of human cornea. To improve its biocompatibility, the IPN hydrogel was coated on its surfaces with another thin layer of different gelatin types. It was found that the hybrid hydrogels developed in this study had the physical properties similar to those of human cornea and their biocompatibilities were also significantly improved, showing its potential to be used as a future biocompatible artificial corneas for 3D printing manufacturing. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-10T21:37:29Z (GMT). No. of bitstreams: 1 U0001-1708202016045800.pdf: 5698279 bytes, checksum: f867937b5e893dc60bb65a5459a206d1 (MD5) Previous issue date: 2020 | en |
| dc.description.tableofcontents | 摘要 I ABSTRACT II 目錄 IV 圖目錄 VII 表目錄 XII 第一章 緒論 1 第二章 文獻回顧 2 2.1 人眼角膜 2 角膜上皮層(Corneal epithelium) 3 鮑曼層(Bowman’s layer) 3 角膜基質層(Corneal stroma) 4 後彈力層(Descemet’s layer) 4 角膜內皮層(Corneal endothelium) 4 2.2 人工角膜 5 2.2.1 波士頓人工角膜(Boston Keratoprosthesis) 5 2.2.2 AlphaCor人工角膜 7 2.3 生物相容性 8 2.4 水膠 9 2.4.1 泊洛沙姆407 (Poloxamer 407) 10 2.4.2 明膠(gelain) 11 2.5 互穿網路水膠(Interpenetrating polymer network hydrogel) 14 2.6 3D列印 15 2.6.1 熔融沉積(Fused Deposition Modeling, FDM) 16 2.6.2 粉末材料選擇性雷射燒結(Selective Laser Sintering, SLS) 17 2.6.3 光敏樹脂選擇性固化(Stereo Lithography Apparatus, SLA) 18 第三章 實驗方法 19 3.1 實驗藥品與儀器 19 3.1.1 實驗藥品 19 3.1.2 實驗儀器 21 3.2 藉由親核醯基取代反應合成末端雙烯化之高分子 22 3.2.1 合成末端雙烯化泊洛沙姆407(P407DA) 22 3.3 水膠製備 24 3.3.1 連續式製備泊洛沙姆/明膠之互穿網路水膠 24 3.4 互穿網路水膠表面改質 25 3.4.1 將明膠塗佈於泊洛沙姆/明膠互穿網路水膠表面 25 3.5 水膠特性測量 26 3.5.1 核磁共振光譜 26 3.5.2 水膠前置液熱流變特性測試 26 3.5.3 透光度測試 26 3.5.4 含水率測量 27 3.5.5 拉伸強度測試 27 3.5.6 掃描式電子顯微鏡 28 3.5.7 水膠細胞貼附實驗 28 3.5.8 半衰減全反射式傅立葉轉換紅外線光譜(ATR-FTIR) 31 3.5.9 水膠植入動物皮下組織實驗 32 3.6 利用3D列印機列印人工角膜 34 第四章 結果與討論 35 4.1 泊洛沙姆/明膠之互穿網路水膠 35 4.1.1核磁共振光譜分析P407之末端烯化率 35 4.1.2水膠前置液熱流變特性測試結果 38 4.1.3透光度 48 4.1.4含水率 50 4.1.5機械性質 51 4.1.6掃描式電子顯微鏡 53 4.1.7細胞貼附實驗 54 4.2 泊洛沙姆/明膠之互穿網路水膠表面改質結果 57 4.2.1 透光度 58 4.2.2 ATR圖譜結果 59 4.2.3 SEM結果 64 4.2.4 細胞貼附實驗 65 4.2.5 水膠植入動物皮下組織實驗 69 4.3積層製造泊洛沙姆/明膠之互穿網路水膠 74 第五章 結論 77 Reference 78 Appendix 84 Part I hydrogel 84 接觸角實驗 84 DSC實驗 85 海藻酸鈉-poloxamer IPN水膠-動物實驗 86 | |
| dc.language.iso | zh-TW | |
| dc.subject | 互穿網路水膠 | zh_TW |
| dc.subject | 積層製造 | zh_TW |
| dc.subject | 3D列印 | zh_TW |
| dc.subject | 人工角膜 | zh_TW |
| dc.subject | interpenetrating network hydrogel | en |
| dc.subject | artificial cornea | en |
| dc.subject | 3D printing | en |
| dc.subject | Multilayer manufacturing | en |
| dc.title | 利用積層製造法製備明膠/泊洛沙姆之互穿網路水膠應用於高生物相容性之人工角膜材料 | zh_TW |
| dc.title | Gelatin/Poloxamer Interpenetrating Network Hydrogel for Biocompatible Artificial Corneas by Using Additive Manufacturing Method | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 108-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 芮祥鵬(Siang-Peng Ruei),楊長謀(Chang-Mou Yang),施博仁(Po-Jen Shih) | |
| dc.subject.keyword | 積層製造,3D列印,互穿網路水膠,人工角膜, | zh_TW |
| dc.subject.keyword | Multilayer manufacturing,3D printing,interpenetrating network hydrogel,artificial cornea, | en |
| dc.relation.page | 89 | |
| dc.identifier.doi | 10.6342/NTU202003784 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2020-08-18 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
| 顯示於系所單位： | 化學工程學系 | |
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