以雷射加工實現磁性粒子虛擬流道

Abstract

微流道晶片的發展目標為晶片尺寸的縮小化、高速的分析和有效的操作檢體。大部分搭配磁性粒子分離特定目標物的傳統微流道晶片其藉須由額外的幫浦系統來做為注入檢體的動力。除此之外，在利用此類的微流道晶片時，可能會因為樣品的殘留堵塞傳輸的流道，因而減少晶片的使用壽命。為了解決這些問題，不須建立在晶片上建立實體流道之「虛擬流道」已經被提出。 在本次的研究中，我們藉由精密雷射加工的方式以鎳磷合金材料製作低成本的虛擬流道元件，藉由永久磁鐵來建立外部磁場磁化虛擬流道，進而影響磁性粒子的運動行為。在研究中顯示以此製作的方式的虛擬流道其可成功引導磁性粒子，其引導結果取決於結構的寬度、厚度與整體虛擬流道的幾何結構設計。研究結果發現不同的設計結構可能產生不同的磁場梯度變化改變粒子移動。

The development goals of microfluidic chips trend to shrink the chip size, high-speed analysis and effect operation of specimen. Most traditional chips with magnetic beads separating particular target refer to the injection of specimen, which acquire extra pump systems for providing power transmission. Another problem for utilizing those traditional microfluidic chips are their clogged and contaminated due to the remains of sample in transport channels, thus decreasing the lifetime of chips. To solve this issue, the virtual channel, which is no entity channels fabricated on a chip, has researched. In this study, we designed a low cost nickel-phosphorus virtual channel device by laser processing and utilized a magnet generating an external magnetic field to magnetize virtual channels thereby affecting the motion of magnetic beads. It showed that the ability of virtual channel successfully guiding magnetic beads depends on the width, thickness and geometrical structure of virtual channel. This finding indicates that diverse channels may generate different magnetic field to change the moving magnetic bead.