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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 陳穎練 | |
dc.contributor.author | Chih Lin | en |
dc.contributor.author | 林芝 | zh_TW |
dc.date.accessioned | 2021-05-19T17:48:46Z | - |
dc.date.available | 2023-01-04 | |
dc.date.available | 2021-05-19T17:48:46Z | - |
dc.date.copyright | 2018-01-04 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-12-21 | |
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Bentley SD, Chater KF, Cerdeno-Tarraga AM, Challis GL, Thomson NR, James KD, Harris DE, Quail MA, Kieser H, Harper D, Bateman A, Brown S, Chandra G, Chen CW, Collins M, Cronin A, Fraser A, Goble A, Hidalgo J, Hornsby T, Howarth S, Huang CH, Kieser T, Larke L, Murphy L, Oliver K, O'Neil S, Rabbinowitsch E, Rajandream MA, Rutherford K, Rutter S, Seeger K, Saunders D, Sharp S, Squares R, Squares S, Taylor K, Warren T, Wietzorrek A, Woodward J, Barrell BG, Parkhill J, Hopwood DA. 2002. Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). Nature 417:141-147. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/7641 | - |
dc.description.abstract | 馬鈴薯瘡痂病(Potato common scab)主要由土傳性放線菌馬鈴薯瘡痂病菌(Streptomyces scabies)引起,是近年來影響台灣馬鈴薯產業甚鉅的細菌性病害。然而目前尚無有效殺菌劑及缺乏抗病植株,而輪作等方法亦無法有效降低病害發生,因此希望使用生物製劑成為替代的防治方法。本研究利用分離自台灣田間健康馬鈴薯之液化澱粉芽孢桿菌(Bacillus amyloliquefaciens) Ba01來拮抗馬鈴薯瘡痂病菌。在培養基對峙培養及掃描式電子顯微鏡的實驗中,發現Ba01能有效抑制馬鈴薯瘡痂病菌之生長分化及產孢。經由影像質譜儀分析,發現三種可能的抑菌物質,分別為surfactin、iturin A和fengycin。在溫室的盆栽實驗中,馬鈴薯的罹病度由55.6 ± 11.1% (只接種馬鈴薯瘡痂病菌) 降至4.2 ± 1.4% (同時接種馬鈴薯瘡痂病菌和澆灌Ba01)。而在田間試驗中,馬鈴薯的罹病度也由14.4 ± 2.9% 降至5.6 ± 1.1%,顯示Ba01有望發展成抗馬鈴薯瘡痂病之生物防治製劑。 | zh_TW |
dc.description.abstract | Potato common scab, which is caused by soil-borne Streptomyces species, is a severe plant disease that results in significant reduction in the economic value of potatoes worldwide. Due to the lack of efficacious pesticides, crop rotations, and resistant potato cultivars against the disease, we investigated whether biological control can serve as an alternative approach. In this study, multiple Bacillus species were isolated from healthy potato tubers, and Bacillus amyloliquefaciens Ba01 was chosen for further analyses based on its potency against the potato common scab pathogen Streptomyces scabies. Ba01 inhibited the growth and sporulation of S. scabies and secreted secondary metabolites such as surfactin, iturin A, and fengycin with potential activity against S. scabies as determined by imaging mass spectrometry. In pot assays, the disease severity of potato common scab was decreased from 55.6 ± 11.1% (inoculated with S. scabies only) to 4.2 ± 1.4% (inoculated with S. scabies and Ba01). In the field trial, the disease severity of potato common scab was reduced from 14.4 ± 2.9% (naturally occurred) to 5.6 ± 1.1% after Ba01 treatment, representing the first documented instance that Bacillus species controls potato common scab in nature. | en |
dc.description.provenance | Made available in DSpace on 2021-05-19T17:48:46Z (GMT). No. of bitstreams: 1 ntu-106-R04633011-1.pdf: 2472831 bytes, checksum: 320e61c5445d838ac477f6449db2ec66 (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 口試委員審定書 .................................................................. i
謝詞 ............................................................................ ii 中文摘要 ....................................................................... iii 英文摘要 ........................................................................ iv 目錄 ............................................................................. v 圖目錄 ......................................................................... vii 表目錄 .........................................................................viii 1. Introduction ............................................................. 1 2. Materials and Methods ................................................... 4 2.1 Strains, growth media, chemicals, and phylogenetic analysis ............... 4 2.2 Disk diffusion assays ......................................................5 2.3 Determination of minimal inhibitory concentrations .........................6 2.4 Determination of fractional inhibitory concentrations ......................7 2.5 Scanning electron microscopy ...............................................7 2.6 Imaging mass spectrometry ..................................................8 2.7 Pot assays .................................................................9 2.8 Field trial ...............................................................11 2.9 Tuber slice assays ........................................................12 3. Results .................................................................13 3.1 B. amyloliquefaciens inhibited the growth and sporulation of S. scabies ...13 3.2 Identification of secondary metabolites secreted from Ba01 that potentially inhibited S. scabies growth ...............................................14 3.3 Ba01 reduced the disease severity of potato common scab in pot assays .... 15 3.4 Ba01 reduced the disease severity of potato common scab in the field ..... 16 4. Discussion .............................................................. 18 5. Tables ...................................................................22 6. Figure legends ...........................................................24 7. Figures ..................................................................29 8. References ...............................................................36 9. Appendix ............................................................... 44 9.1 Supplementary Materials and Methods ..................................... 44 9.2 Supplementary Tables .................................................... 50 9.3 References .............................................................. 52 9.4 Supplementary Figures ................................................... 53 9.5 殺真菌劑得克利能抑制馬鈴薯瘡痂病原細菌 .................................. 57 | |
dc.language.iso | en | |
dc.title | 利用液化澱粉芽孢桿菌Ba01防治馬鈴薯瘡痂病菌 | zh_TW |
dc.title | Biological control of potato common scab
by Bacillus amyloliquefaciens Ba01 | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蔡佳欣,謝奉家,曾敏南,楊玉良 | |
dc.subject.keyword | 液化澱粉芽孢桿菌,Ba01,生物製劑,馬鈴薯瘡痂病菌,馬鈴薯瘡痂病, | zh_TW |
dc.subject.keyword | Bacillus amyloliquefaciens,Ba01,biocontrol agent,Streptomyces scabies,potato common scab, | en |
dc.relation.page | 82 | |
dc.identifier.doi | 10.6342/NTU201704490 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2017-12-22 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
顯示於系所單位: | 植物病理與微生物學系 |
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