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DC 欄位 | 值 | 語言 |
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dc.contributor.author | Chuan-Hsiu Cho | en |
dc.contributor.author | 卓娟秀 | zh_TW |
dc.date.accessioned | 2021-07-01T08:20:56Z | - |
dc.date.available | 2021-07-01T08:20:56Z | - |
dc.date.issued | 1998 | |
dc.identifier.citation | 王世彬、林讚標、簡慶德。1995。林木種子儲藏性質的分類。林業試驗所研究報告季刊10(2):255-276。 王裕文、郭華仁。1998。種子分析巨集程式(網路版)。http://Seed.agron.ntu.edu.tw/tool/tool.htm 林讚標。1992。數種殼鬥科植物儲藏性質-赤皮、青剛櫟、森氏櫟與高山櫟。林業試驗所研究報告季刊10(1):9-13。 林讚標、吳濟琛。1991。樟科種子儲藏性質與發芽性質一、土肉桂與長葉木薑子。林業試驗所研究報告季刊6:339-344。 林讚標、陳美清。1993。大葉楠種子不耐乾燥。林業試驗所研究報告季刊8:339-344。 林讚標、簡慶德。1995。六種楨楠屬植物種子不耐旱特性。林業試驗所研究報告季刊10(2):217-226。 蔡淑華。1973。植物解剖學。國立編譯館。P.284 簡慶德、楊佳如。1997。長葉木薑子種子成熟度影響種子的儲藏能力。台灣林業科學12(3):369-272。 鍾永立、張乃航。1990。台灣重要林木種子技術要覽。林業叢刊號35號。林業試驗所印行。 Berjak, P., M. Dini, and N. W. Pammenter. 1984. Possible mechanisms under lying the differing dehydration responses in recalcitrant and orthodox seeds: desiccation - associated subcellular change in propagules of Avicennia marina. Seed Sci. Techonol.12: 365-384. Berjak, P., J. M Farrant, D. J. Mycock, N. W. Pammenter. 1990. Recalcitrant (Homoiohydrous) seeds; the enigma of their desiccation sensitivity. Seed Sci Technol 18:297-310 Berjak, P., N. W. Pammenter, and C. W. Vertucci. 1992. Homoiohydrous (recalcitrant) seeds: development status, desiccation sensitivity and the state of waetr in the axes of Landolphia kirkii Dyer. Planta 186:249-261 Berjak P., C. W. Vertucci and N. W. Pammenter. 1993. Desiccation-sensitive (recalcitrant) seeds: effects of development status and dehydration rate on characteristics of water and desiccation-sensitivity in Camellia sinensis. Seed Science Research. 155-166. Berjak, P. et. al. 1993. Effect of development status and dehydration rate on characteristics of water and desiccation-sensitivity in recalcitrant of Camellia sinensis. Seed Science Research 1-13. Berjak, P., K. J. Bradford, D. A. Kovach and N. W. Pammenter. 1994. Differential effcets of temperature on ultrastructure response to dehydration in seeds of Zizania palustris. Seed science Research 4:111-121. Bonner, F. T.1990. Storage of seeds: potential and limitation for germplasm conservation. For, Ecol. Manage. 35:35-43 Bozla, J.J. and L.D. Russell. 1992. Electron Microscopy. Jones and Bartlett Publisher. P20 Cram, W. H. and H. A. Wrden. 1957. Maturity of white spruce cones and seeds. For. Sci. 3:263-269 Chin H. F, E. H. Roberts. 1980. Recalcitrant crop seeds. Kuala Lumpur: Tropical Press.SDN. BHD Chien, C. T. and T. P. Lin. 1997. Effect of harvest date on storability of desiccation-sensitive seeds of Machilus kusanoi Hay. Seed Sci. and Technol. 25.000-000. Dodd, M.C., J. V. Staden and M. T. Smith. 1989. Seed development in Podocarpus henkelii: an ultrastructure and biochemical study. Annals of Botany. 64:297-310. Editorial Committee of the Flora of Taiwan. 1996. Flower of Taiwan. Second Edition Espindola, L., N. M. Samen, F. Corbineau and D. Come. 1994. Cellular and metabolic damage induced by desiccation in recalitrant Araucaria angustifolia embryos. Seed Sci. Research. 4:193-201. Farrant, J. M., P. Berjak. And N. W. Pammenter. 1985. The effect of drying rate on viability retentyon of recalcitrant propagules of Avicennia marina. South Afr. J. of Bot. 51:432-428. Farrant, J. M., N. W. Pammenter, P. Berjak. 1986. The increasing desiccation sensitivity of recalcitrant Avicennia marina seeds with storage time. Physiol. Plant. 67:291-298. Farrant, J. M., N. W. Pammenter, P. Berjak. 1988. Recalcitrant - a current assessment. Seed Sci. Technol. 16:155-166. Farrant, J. M., N. W. Pammenter, P. Berjak. 1989. Germination-associated events and the desiccation sensitivity of recalcitrant seed--- a study on three unrelated species. Planta 178:189-198. Farrant, J. M., N. W. Pammenter, P. Berjak and W. Christina. 1997. Subcellular organization and metabolic activity during the development of seeds that actin different levels of desiccation tolerance. Seed Sci. Research 7:135-144. Farrant, J. M., N. W. Pammenter and P. Berjak. 1992. Development of the recalcitrant (homoiohydrous) seeds of Avicennia marina: anatomical ultrastructural and biochemical events associated with development from histo differantion to maturation. Ann. of Bot. 70:76-86. Farrant, J. M., N. W. Pammenter and P. Berjak. 1993a. A contribution to an understanding of desiccation tolerance from a study of desiccation-sensitive. seeds species pp 715-722 in C?me, D., and Corbineau, F. (Eds) Proceedings of the Fopuerth International Workshop on seeds: basic and applied aspects of seed biology. Paris AFSIS. Farrant, J.M., P. Berjak and N. W. Pammenter. 1993b. Studies on the development of the desiccation-sensitive (recalcitrant) seeds of Avicennia marina (Forsk.) Vierh; the acquisition of germinability and response to storage and dehydration. Ann. of Bot. 71:405-410. Finch-Savage W.E. 1992. Seed development in the recalcitrant species Quercus robur L: germinability and desiccation tolerance. Seed Sci. Research 2:17-22. Finch-Savage W. E. and Clay H.A. 1994. Water relations of germination in the recalcitration seeds of Quercus Robur L. Seed Sci. Research 4:315-322. International Seed Testing Association. 1993. International rules for testing. Seed Sci. and Techmnol. 21:43-46. Koster K. L., A. C. Leopold. 1988. Sugars and desiccation tolerance in seeds Planta physiol. 88:829-832. Kovach, D. and K. J. Bradford. 1992. Imbibitional damage and desiccation tolerance of wild rice (Zizania Palustris) seeds. J. of Exp. Bot. 43: 747-757. Lin.T. P. and M. C. Chen. 1993. Desiccation intolerance in seeds of Michellia kusanoi Hay. Bulletin of Taiwan Forestry Research Institute, 8: 143-147. (in Chinese) Lin T. P. and J. C. Wu. 1995. Seed storage behavior of Michellia compressa (Max.) Sargent. Seed Sci. Technol. 23:309-319. Leprince, O. 1993. The mechanisms of desiccation tolerance in developing seeds. Seed Sci. Research 3:231-246. Martin A. C. 1946. The comparative internal morphology of seed. The Americal midland naturalis. 36:513-660. Motete, N., N. W. Pammenter, P. Berjak and J. C. Fredwric. 1997. Response of the recalcitrant seeds of Avicennia marina to hydrated storage: events occuring at the root primordia. Seed Sci. Research 7:167-178. Mycock, D. J. and P. Berjak. 1990. Fungal contanminants associated with several homoiohydrous (Recalcitrant) seed species. Phyto- phylactia 22:413-418. Pammenter, N. W., J. M. Farrant, M. T. Smith and P. Berjak. 1994. Recalcitrant seeds: short-term storage effects in Avicennia marina (Forsk) Virh may be germination - associated. Ann. of Bot. 54:843-846. Pammenter, N. W., C. W. Vertucci and P. Berjak. 1991. Homoihydrous (recalcitrant) seeds: dehydration, the state of water and viability characteristics in Landolphia kirkii. P. Physiol. 96:1093-1098. Pammenter, N. W., P. Berjak, J. M. Farrant, M. T. Smith and G. Ross. 1994. Why do store hydrate recalcitrant seeds die Seed Sci. Research 4:187-191. Roberts, E. H., M. W. King, R. H. Ellis. 1984. Recalcitrant seeds: their recognition and storage. In Holden JHW, Williams JT. eds. Crop genetic resource: conservation and evaluation. London: Allen and Unwin pp38-52. Rrobert, R.T. and E. R. Bzrierley. 1989. Desiccation intolerance in seeds of Zizania palustria is not relted to developmental age or the duration of postharvest storage. Ann. of Bot. 64:669-674. Tompsett., P. B. and H. W. Pritchard. 1993. Water status change deveolopment in relation to the germination and desiccation tolerance of Aesculus hippocastanum L., seeds. Ann of Bot. 71:107-116. Vertucci, C. W. and A. C. Leopold. 1987. The relationship between water binding and desiccation tolerance in tissues. P. Physiol. 85:232-238. Vertucci, C. W. and E. E. Roots. 1990. Theoretical basis of protocols for seed storage. P. Physiol. 94:1019-1023. Vertucci C. W. 1993. Predicting the optimum storage condiation for seeds using thermodynamic principles. J. of Seed Technol. 17(2): 41-53. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76383 | - |
dc.description.abstract | 本論文是以波羅蜜(Artocarpus heterophyllus Lam.)、大葉楠(Machilus japonica var. kusanoi Hayata)、青剛櫟(Cyclobalanopsis glauca (Thunb.) Oerst.)、長葉木薑子(Litsea acuminata (Blume) Kurata)與銀合歡(Leucaena leucocephala Lam.)等台灣低海拔不同生育地的林木種子為材料,觀察新鮮種子外形、胚的主軸(embryo axes)的形態與解剖特色及種子中不可溶儲藏物質的化學成份,並探討乾旱處理對種子存活力的影響。此外,亦觀察五種種子的胚軸、胚芽及胚根頂端分生組織之微細構造的差異及比較各種胚的主軸受到乾燥處理後在微細構造上的變化。 由實驗結果顯示五種種子的體積以波羅蜜最大而銀合歡最小,然而此二種子胚的主軸大小相似,其餘三種的胚軸較小;各胚軸在種子成熟採收時大多已發育,但可見4?6枚初生葉包被在胚芽外,然而青剛櫟僅為一團尚未明顯分化的細胞。大葉楠、青剛櫟及長葉木薑子等種子的子葉及胚軸內均具有分泌細胞或油細胞,波羅蜜的胚軸與子葉中含有乳汁管,而單寧細胞則普遍存在於各種子路軸的皮層與表皮組織、初生葉及根帽組織中。 種子胚軸中不可溶性的儲藏物質,以澱粉粒、蛋白質體及油滴為主。波羅蜜、大葉楠與長葉木薑子的胚軸基本組織細胞中可見澱粉體,而油滴則分佈於胚軸組織的細胞質中以及胚芽與胚根分生組織細胞的邊緣。銀合歡胚的主軸細胞的邊緣與蛋白質體的外圍密佈小油滴。除銀合歡外,各胚軸中較少見蛋白質體的存在。 成熟採收的五種種子的含水率以波羅蜜(61.03%)最高,而銀合歡(6.7%)最低;就新鮮種子的發芽率而言,則分別為60%、95.04%、96%、93.33%、94%。經過氯化鋰飽和鹽溶液乾燥處理後的種子,經萌發實驗的結果得知,波羅蜜、大葉楠、青剛櫟、長葉木薑子各種子若分別失去其含水量的6%、7.5%、17%、35%,則發芽率急速降低;若儲存於4℃的環境下,各種子保存活力的時間以銀合歡最長而波羅蜜最短。故而這些種子對乾燥的敏感性,由強到弱分別為波羅蜜、大葉楠、青剛櫟、長葉木薑子、銀合歡。 觀察各種子胚芽與胚根頂端分生組織細胞的微細構造,顯示此二部份的細胞質濃厚,細胞核大而明顯,佔細胞空間的20?30%;細胞質中液胞所佔的比例則依種類的不同而大小不一。在粒線體、內質網、色素體等胞器發育狀況與分佈的觀察中,銀合歡的細胞質中少見各種胞器,其餘四種則顯見各胞器的存在與活動的跡象。 當種子受到乾旱產生水分逆境時,在種皮、子葉與胚的主軸外觀上出現明顯的皺縮現象,含水率也明顯下降。透過微細構造的觀察,胚芽分生組織細胞隨著含水率的下降,出現液胞癒合或液胞膜破裂,各胞器的形態與相對空間位置改變、細胞核與核仁變形、原生質質離與細胞邊緣出現小囊泡化等現象;在胚根的分生組織細胞內有類似的變化但較不明顯,而根帽部份的細胞則出現明顯的原生質離現象,細胞中液胞化的現象也較胚根頂端分生組織明顯。 對乾燥敏感性較低的種子,通常其胚芽及胚根頂端分生組織細胞的細胞質較濃厚,液胞極小或沒有,細胞儲藏物以油滴與蛋白質體為主;隨著乾燥敏感性提昇,胚主軸頂端分生組織細胞的液胞化現象增加,各胞器亦呈相對程度的增加或出現活化狀態,並與種子萌發的第一發芽時間成正相關。本研究的五種種子中,對乾燥敏感性最強的波羅蜜種子的胚芽與胚根頂端分生組織細胞內具有高度液胞化的現象,與其他敏感性較弱的種子間呈現顯著差異;而就其他乾燥敏感性程度不同的種子與銀合歡種子比較,其液胞化程度亦呈顯著差異,但彼此間則差異不大。 | zh_TW |
dc.description.abstract | Artocarpus heterophyllus Lam., Machilus japonica var. kusanoi Hayata, Cyclobalanopsis glauca (Thunb.) Oerst, Litsea acuminata (Blume) Kurata and Leucaena leucocephala Lam. are five woody species distrributed in the low forests of Taiwan. This thesis investigated their seed morphology and the anatomical characteristics, including the composition of insoluble storage materials, the ultrastructure of plumule and apex of radicle of the embryo axes. Besides the effects of desiccation on the seed viability and the ultrastructure of embryo axes were studied. The results showed that Artocarpus has the biggest seed mass, however, the size of its embryo axes is similar to that of Leucaena. Among the five species the seed of Leucaena is the smallest one. With the exception of Cyclobalanopsis, all the other embryo axes in matured seed are full-grown and generally with 4-6 primary leaves. In the cotyledons and embryo axes of the seeds of Machilus, Cyclobalanopsis and Litsea there are secretory mucilage or oil cells. The seeds of Artocarpus obviously contain laticifers in the cotyledons and embryo axes. However, tannic cells are ubiquitously distributed in the cortex and epidermis of embryo axes, primary leaves and root caps of the seeds. The predominant compositions of insoluble storage materials in the seeds of these five species are starch grains, protein bodies and oil droplets. The starch grains are distributed in the ground tissue of embryo axes of Artocarpus, Machilus and Litsea.. The oil droplets are located in the cytoplasm of the cells of embryo axes and in the marginal areas of the cells of plumule and radicle apex. Nevertheless, in the seeds of Leucaena, the oil droplets are mostly observed around the proteins bodies in the peripheryn of the cells in he embryo axes. The highest and lowest moisture contents of mature seed are fong in Artocarpus (61.03%) and Leucaena (6.7%) respectively, and the rates of germination are 60% (Artocarpus), 95.04% (Machilus), 96% (Cyclobalanopsis), 93.33% (Listea) and 94% (Leucaena). When the seeds were lost dehydrated 6% (Artocarpus), 7.5% (Machilus), 17% (Cyclobalanopsis), 35% (Lacumunata) of their moisture contents by the saturated lithium chloride (LiC1), the rate of germination decreased dramatically. Among the five woods, the seed of Lacumunata are the most resistant to low temperature storage (4℃) and seed of Artocarpus are more susceptable to low temperature treatment. These results suggest that the degrees of desiccation susceptibility of these seeds are Artocarpus, Machilus, Cyclobalanopsis, Litsea and Leucaena. The ultrastructural study on the seeds showed the cells of plumule and radicle apex has dense cytoplasm, and a large nucleus occuping 20-30% of the cell volumn. The size of vacuoles in the cytoplasm widely varies with the species. In the cytoplasm of Leucaena, the mitochondria, endoplasmic reticulum and plastids are seldom observed, however, in other species these organelles are ubiquitous distributed and metabolic active. During the drought stress, the seed coat, cotyledon and embryo axes are obviously shrunk. The ultrastructural change in the cells of plumule apical meristem are fusion or lysis of tonoplast, deformation of nucleus and nucleolus, shrinkage of protoplasm, and formation of small vesicles in the cell periphery. A similar, but less significant change was observed in the radicle apex, however, the cells in the radicle showed dramatic plasmolysis and vacuolation. In summary, the present study suggested that the more resistant to the desiccation a seed, the more densely of the cytoplasm in the cells of apex of plumule and radicle. Besides, in the desiccation resistant seeds the vacuoles are small or lack, and the predominant storage compounds are oil droplets and protein bodies. The vacuolation of the apical meristematic cells and the activity and the quantity of organelles in the cells of embryo axes are increased with the desiccation susceptibility of the seeds. In the seeds of the five studied species, highly vacuolation was only found in the plumule and radicle of Artocarpus seed, and the degree of vacuolation in the other four species is not significantly different, but is highly associated with their desiccation susceptibility. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:20:56Z (GMT). No. of bitstreams: 0 Previous issue date: 1998 | en |
dc.description.tableofcontents | 附表目錄……………………………………………………………………Ⅰ 附圖目錄……………………………………………………………………Ⅱ 圖版目錄……………………………………………………………………Ⅲ 中文摘要……………………………………………………………………Ⅴ 英文摘要……………………………………………………………………Ⅶ 壹、前言……………………………………………………………………1 貳、材料與方法 一、材料:種子的採集與處理……………………………………………5 二、種子耐旱性的檢測……………………………………………………6 三、新鮮及乾燥後種子外部與胚外形與解剖構造的觀察………………9 四、新鮮及乾燥種子微細構造的觀察……………………………………11 五、形態計量與分析………………………………………………………11 參、結果 一、種子的形態構造與組織化學檢測……………………………………12 (一)種子的外部形態及胚的主軸的形態與構造 1.波羅蜜……………………………………………………………………12 2.大葉楠……………………………………………………………………12 3.青剛櫟……………………………………………………………………13 4.長葉木薑子………………………………………………………………14 5.銀合歡……………………………………………………………………14 (二)組織化學檢測 1.波羅蜜……………………………………………………………………16 2.大葉楠……………………………………………………………………16 3.青剛櫟……………………………………………………………………17 4.長葉木薑子………………………………………………………………17 5.銀合歡……………………………………………………………………17 二、種子對乾燥敏感性的研究 (一)新鮮種子的含水率與發芽率的測定與比較 1.波羅蜜……………………………………………………………………18 2.大葉楠……………………………………………………………………18 3.青剛櫟……………………………………………………………………19 4.長葉木薑子………………………………………………………………19 5.銀合歡……………………………………………………………………20 (二)新鮮種子失水速率的測定與比較 1.波羅蜜……………………………………………………………………21 2.大葉楠……………………………………………………………………21 3.青剛櫟……………………………………………………………………21 4.長葉木薑子………………………………………………………………21 (三)乾燥種子含水率與發芽率的測定與比 1.波羅蜜……………………………………………………………………22 2.大葉楠……………………………………………………………………23 3.青剛櫟……………………………………………………………………23 4.長葉木薑子………………………………………………………………24 5.銀合歡……………………………………………………………………24 (四)種子耐旱程度的比較………………………………………………25 三、胚主軸頂端分生組織的微細構造 (一)新鮮種子微細構造的觀察與比較…………………………………26 1.胚芽頂端分生組織 (1).波羅蜜…………………………………………………………………26 (2).大葉楠…………………………………………………………………26 (3).青剛櫟…………………………………………………………………27 (4).長葉木薑子……………………………………………………………27 (5).銀合歡…………………………………………………………………28 2.胚根頂端分生組織 (1).波羅蜜…………………………………………………………………29 (2).大葉楠…………………………………………………………………29 (3).青剛櫟…………………………………………………………………30 (4).長葉木薑子……………………………………………………………30 (5).銀合歡…………………………………………………………………31 (二)乾燥處理對種子胚微細構造的影響 1.波羅蜜種子:……………………………………………………………32 2.大葉楠:…………………………………………………………………33 3.青剛櫟:…………………………………………………………………34 4.長葉木薑子:……………………………………………………………35 圖版標示說明………………………………………………………………55 圖版…………………………………………………………………………56 肆、討論……………………………………………………………………97 伍、引用文獻………………………………………………………………110 | |
dc.language.iso | zh-TW | |
dc.title | 五種林木種子對乾燥的敏感性與微細構造 | zh_TW |
dc.title | Desiccation Sensitivity and Ultrastructure of Five species of Tree Seeds | en |
dc.date.schoolyear | 86-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 128 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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