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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76367完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 李玲真 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:20:46Z | - |
| dc.date.available | 2021-07-01T08:20:46Z | - |
| dc.date.issued | 1980 | |
| dc.identifier.citation | 1. Angelo, A.J. St., R.L. Ory, and H.J. Hansen. 1969. Localization of an acid proteinase in hempseed. Phytochemistry 8:1135-1138. 2. Ashton, F.M., D. Penner, and S. Hoffman. 1968. Effect of several herbicides on proteolytic activity of squash seedlings. Weed Sci. 16:169-171. 3. Baur, J.R., R.W. Bovey, P.S. Baur, and Z. Seffy. 1969. Effect of paraquat on the ultrastructure of mesquite mesophyll cells. Weed R Res. 9:81-85. 4. Beevers, L. 1968. Protein degradation and proteolytic activity in the cotyledons of germination pea seed. Phytochemistry 7:1837-1844. 5. Bernfeld, P. 1955. Amylases, α and β , In “Methods in enzymology (S.P. Colowick and N.O. Kaplan). Vol.I. pp.149-158. Academic Press. 6. Burger, W.C. and H.W. Siegelman. 1966. Physiol. Plantarum. 19:1089. 7. Chen, Y. L. 1980. Current status of herbicides in Taiwan. Weed Science Buletin 1:60-62. 8. Chrispeels, M.J., and D. Boulter, 1975. Control of storage protein metabolism in the cotyledons of germinating mung beans:role of endopeptidase. Plant physiol. 55:1031-1037. 9. Chrispeels, M.J., and I.E. Varner. 1967. Gibberellic acid- enhanced synthesis and release of α-amylase and ribonuclease by isolated barley aleurone layers. Plant Physiol. 42:398-406. 10. Davis, B.J. 1964. Disc-electrophoresis-II, Method and application to human serum proteins. Ann. N. Y. Acad. Sci. 121:404-427. 11. Devlin, R.M. and R.P. Cunningham. 1970. The inhibition of gibberellic acid induction of barley endosperms by certain herbicides. Weed Res. 10:316-320. 12. Dhillon, N.S. and J.L. Anderson. 1972. Morphological, anatomical and biochemical effects of propachlor on seedling growth. Weed Res. 12:182-189. 13. Duke, W.B., F.W. Slife, J.B. Hanson, and H.S. Butler. 1975. An investigation on the mechanism of action of propachlor. Weed Sci.23:142-147. 14. Erlanger B.F., N. Kokowsky, and W. Cohen, 1961. Arch. Biochem. Biophys. 95:271. 15. Eshel, Y. 1969. Phytotoxicity, and site of uptake of alachlor. Weed Sci. 17:441-444. 16. Galston, A.W. and L.Y. Dalberg, 1954. The adaptive formation and physiological significance of indoleacetic acid oxidase. Am.J. Botany. 41:373-380. 17. Galston, A.W., and P.J. Davies, 1969. Homonal regulation in higher plants. Science 163:1288-1297. 18. Gove, J.P. and M.C. Hoyal, 1974. The isozymic similarity of indoleacetic acid oxidase to peroxidase in birch and horseradish. Plant Phvsiol. 56:684-687. 19. Haard, N. F., and M. Marshal. 1976. Isoperoxidase changes in soluble and particulate fractions of sweet potato root resulting from cut injury, ethylene and black rot infection. Physiol Plant Pathol.8:195-205. 20. Halevy, A.H. 1963. Interaction of growth retarding compounds and gibberellin on indoleacetic acid oxidase and peroxidase of cucumber seedlings. Plant Physiol. 38:731-737. 21. Hamm, P.C. 1974. Discovery, development, and current status of the chloroacetamide herbicides. Weed Sci. 22:541-545. 22. Hamner, C.L. and H.B. Tukey. 1944. The herbicidal action of 2,4-D and 2, 4, 5-Tonbindweed. Science 100:154-155. 23. Hardy, S.J.S.,, C.G. Kurland, P. Voynow, and G. Mora. 1969. The ribosome proteins of E. coli. I. Purification of the 30S robosomal proteins. Biochemistry 8:2897-2905. 24. Harris, N., and A.D. Dodge. 1972. The effect of paraquat on flax cotyledon leaves:Changes in fine structure. Planta 104:201-209. 25. Harris, N. and A.D. Dodge. 1972. The effect of paraquat on flax cotyledon leaves:Physiological and biochemical changes. Planta 104:210-219. 26. Hickey, J.S. and W.A. Krueger, 1974. Alachlor and 1, 9-napthalic anhydride effects on sorghum seedling development. Weed Sci. 22:86-90. 27. Horng, L.C. and L.S. Len. 1977. Weed flora in rice paddy fields in Taiwan. Proceedings of the 5th Asian-Pacific Weed Science Conference, Jakarta, Indonesia. 1:116-122. 28. Jacob, J.B., and J.E. Varner, 1967. Gibberellic acid-induced synthesis of protease by isolated aleurone layers of barley. Plant Physiol. 42:1596-1600. 29. Jaworski, E.G. 1956. Biochemical action of CDAA, a new herbicide. Science. 123:847-878. 30. Jaworski, E.G. 1969. Analysis of the mode of action of herbicidal α -chloroacetamides. J. Agr. Food Chem. 17:1650170. 31. Jones, D.W. and C.L. Foy. 1971. Herbicidal inhibition of GA-induced synthesis of α-amylase. Weed Sci. 19:595-597. 32. Jones, R.A., B.A. Larkins, and C.Y. Tsai. 1977. Storage protein synthesis in Maize. II. Reduced synthesis of a major zein component by the opaque-2 mutant of Maize. Plant Physical. 59:525-529. 33. Kunitz, M. 1947. Crystalline soybean trypsin inhibitor, II. General properties. J. Gen. Physiol. 30:291-310. 34. Lowry, O.H., N.Y. Rosebrough, A.L. Farr, and R.J. Randall.1951. Protein measurem ent with Folin -phenol reagent. Biochem.J. 50:298-302. 35. Mann, J.D., L.S. Jordan, and B.E. Day. 1965. A survey of herbicides for their effect upon protein synthesis. Plant Physiol. 40:840-843. 36. Matile, p. 1975. In “Plant Biochemistry” 3rd ed. pp. 184-224. 37. Matile, P. and H. Moor. 1968. Vacuolation:origin and development of the lysosomal apparatus in root-tip cells. Planta 80:159-175. 38. Matile, P. and F. Winkenbach. 1971. Rinction of lysosomes and lysosomal enzymes in the senescing corolla of the morning glory.J. Exp. Bot. 22:759-771. 39. Mets, L., and L. Bogorad. 1774. Two-dimensional polyacrylamide gel electrophoresis:An improved method for ribosomal proteins. Anal. Biochem. 57:220-210. 40. Moore, S., and W.H. Stein. 1954. A modified ninhydrin reagent for the photometric determination of amino acids and related compounds. J. Biol. Chem. 221;907-913. 41. Moreland, D.E., S.S. Malhotra, R.D. Gruenhagen, and E.H. Shokraii. 1969. Effects of herbicides on RNA and protein syntheses. Weed Sci. 17:556-363. 42. Marsaiah, D.B. and R.G. Harvey. 1977. Alachlor and gibberellic acid interaction on corn tissues. Weed Sci. 25:197-199. 43. Penner, D. 1968. Herbicidal influende of amylase in barley and squash seedlings. Weed Sci. 16:519-522. 44. Pillai, P., D. E. Davis, and B. Truelove. 9979. Effects of metochlor on germination, growth, leucine uptake, and prtein synthesis. Weed Sci. 27:634-637. 45. Ram Chandra, G., and J. E. Varner, 1965. Gibberellic acid- controlled metabolism of RNA in aleurone cells of barley. Biochem. Biophys. Acta 108:583-592. 46. Rao, V.S., and W.B. Duke. 1976. Effect of alachlor, prochlor, and prynachlor on GA3-induced production of protease and amylase. Weed Sci. 24:616-618. 47. Rao, V.S., and W.B. Duke, 1977. Biochemical mechanisms of action of acetanilide herbicides. Weed Abstr. p.451. 48. Sabatini, D.D., K.G. Bensch, and R.J. Barnett. 1962. New fixatives for cytological and cytochemical studies. in Fifth International Congress for Electron Microscopy (Phila.) Vol. II (Academic Press, N.Y. pp. 1-3. 49. Shannon, L.M. 1968. Plant isozymes. Ann. Rev. Plant Physiol.19:187-210. 50. Siegel, B.Z., and A.W. Galston. 1967. The isoperoxidases of Pisum sativum. Plant Physiol. 42:221-225. 51. Spurr, A.R. 1969. “A low-viscosity epoxy resin embedding medium for electron microscopy:J. Ultrast. Res. 26:31-43. 52. Yemm, E.W., and A.J. Willis, 1954. The estimation of carbohydrate in plant extracts by anthrone. Biochem. J, 57:508-514. 53.蔡淑華，1975，“植物組織切片技術網要”。 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76367 | - |
| dc.description.abstract | 以丁基拉草對水稗種子作萌芽前處理，觀察種子萌芽，及幼苗生長的變化。丁基拉草不抑制水稗種子萌芽，但是延後萌芽的時間；對幼苗的生長則有明顯的抑制。在形態方面，處理組的幼苗發生兩大變化：(1）第一片葉子不突出子葉鞘，(2)根的生長被抑制。由解剖切片觀察幼苗根尖的細胞，處理組的細胞發生囊泡化（vesiculation）現象，逐漸擴大的液泡將細胞質嵌入（invagination)，最後細胞趨向崩解。在生化方面，處理組的幼苗在澱粉分解酵素的活性受到50%的抑制，胚乳碳水化合物的分解也被抑制；在蛋白質分解酵素則隨不同受質而有不同的變化，以牛乳蛋白為受質時，酵素活性被抑制，同時胚乳蛋由質的分解也被抑制，而以BAPA為受質時，酵素活性不被抑制，同時處理組的幼苗的可溶性胺基酸含量逐漸增加，顯示丁基拉草使水稗幼苗細胞發生自我分解（autolysis)；在過氧化酵素，則活性被抑制，而異構?的型態沒有明顯的變化。 水稗種子在丁基拉草溶液中播種36小時，抽取其中核糖體，以連續蔗糖濃度梯度法分離的結果顯示丁基拉草在水稗萌芽早期就抑制形成polysome的能力。 水稗種子在不同濃度的丁基拉草溶液中播種12小時，然後經不同時間的水沖洗，再移植到水中生長，觀察其幼苗的形態，顯示即使以低濃度（6ppm）的丁基拉草處理，經過較長時間的沖洗也不能除去丁基拉草的抑制作用，只能恢復一部分生長。可能丁基拉草對水稗萌芽早期就有相當的抑制作用。由以上的觀察推測丁基拉草對水稗的作用機制是多方面的，抑制蛋白質合成是其中之一。 | zh_TW |
| dc.description.abstract | The effects of butachlor [2-chloro-2’, 6’-diethyl-N-(butoxymethyl) acetanilide] on seed germination of barnyardgrass (Echinochloa crusgalli Beauv.) and subsequent seedlying growth were investigated. Butachloi did not affect seed germination up to a concentration of 100 ppm tested in this study, but delayed the time of coleorhiza emergence through the pericarp of seed for 12 hours. Further seedling growth was severly inhibited. Emergence of the primary leaf from the coleoptile and root growth were inhibited by butachlor as low concentration as 6 ppm. Light and electron microscopic studies indicated that butachlor induced cellular autophagy of root tissues. The development of amylase and protease activities were prevented so that the degradation of reserve carbohydrates and proteins in endosperm was affected by butachlor during 7 days of germination. Protein synthesis namely polysome formation was also inhibited during the first 36 hours of germination. Time course studies on the seedling growth revealed that the inhibition was observed during the initial 12 hours of seed imbibition. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:20:46Z (GMT). No. of bitstreams: 0 Previous issue date: 1980 | en |
| dc.description.tableofcontents | 中文摘要 英文摘要 一、緒言……………………………………1 二、實驗材料與方法……………………………………5 (一)植物材料及除草劑之選擇……………………………………………5 (二)化學藥品…………………………………………5 (三)緩衝液和試劑……………………………………………6 (四)除草劑處理植物的方法……………………………………6 (五)發芽率及形態的比較……………………………………7 (六)鮮重、乾重的測定……………………………………8 (七)貯藏養分的定量分析…………………………………………8 (八)分解酵素的粹取及其活性的測定……………………………………9 1 澱粉分解酵素……………………………………9 2 蛋白質分解酵素………………………………………………10 (九)過氧化酵素的粹取，活性的測定及電泳的分析……………………………………11 (十）蛋白質含量的測定………………………………………12 (十一)鹼性蛋白質的分離及二度膠體電泳分析……………………………………13 (十二)核糖體的抽取及分析…………………………15 (十三)埋臘切片的製備……………………………………15 (十四)穿透式電子顯微鏡的切片……………………………………………16 三、結果…………………………………………………17 (一)發芽率及形態的比較……………………………………17 (二)鮮重及乾重的變化……………………………………18 (三)貯藏養分在萌芽期間的變化情形……………………………………19 1 可溶性糖量的變化……………………………………19 2 可溶性胺基酸的變化……………………………………20 (四)種子萌芽期間，兩種分解酵素的變化……………………………………20 1 澱粉分解酵素……………………………………20 2 蛋白質分解酵素……………………………………21 (五)種子萌芽期間過氧化酵素的活性及電泳分析……………………………………22 (六)鹼性蛋白質的二度膠體的電泳分析……………………23 (七)核糖體的變化………………………………………23 (八)解剖方面的變化…………………………………………24 四、討論……………………………………55 五、引用文獻……………………………………61 | |
| dc.language.iso | zh-TW | |
| dc.title | 丁基拉草對水稗種子萌芽及幼苗生長的影響 | zh_TW |
| dc.title | Effects of Butachlor on Seed Germination and Seedling Growth of Echinochloa crus-galli:Morphological,Anatomical and Biochemical Studies. | en |
| dc.date.schoolyear | 68-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 74 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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