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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76328完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 林靖愉 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:20:21Z | - |
| dc.date.available | 2021-07-01T08:20:21Z | - |
| dc.date.issued | 1997 | |
| dc.identifier.citation | 何家菀，陳明惠，何振榮， 1996。小定鞭藻毒素的分離與鑑定，水生生物學報，第20卷， 41-48。
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N-alleylsecoboldine as a novel antioxidant against per-oxidative damage. Europe. J. Pharmacol. 303: 129-139. Tringali, C., and Piattelli, M. 1982. Two chroinone derivatives from the brown alga Zonaria tournefortii. Tetrahedron Lett.23: 1509—1512. Wang, S. Y., and Faust, M. 1990. Seasonal changes of membrane lipids in apple shoots. J. Ain. Soc. Hortic. Sd. 115(3):462—467 .Wehrli, F. W, and Wrthlin, T. 1980. The spectral parameters. In: Introduction of Carbon—13. ‘ehrli, F. VI. and irthlin, T. (eds) . pp. 45—47. Heyden & Son press, London. ‘Whitaker, B. D. 1992. Glycerolipid—fatty—acid desaturase deficiencies in chloroplasts from fruits of CapsicuinannuuinL. Planta 187(2): 261—265. York, W S., Darvill, A. G., McNeil, M., Stevenson, T. T., and Albersheim, P. 1986. Isolation and characterisation of plant cell wall components. lethods Enzyinol., 118: 3—40. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76328 | - |
| dc.description.abstract | 第一部份 東方粉枝藻(Liagora orientalis)及波氏粉枝藻(Liagora boergesnii)雙半乳糖基二醯基甘油的分離與結構鑑定 實驗室大量培養的東方粉枝藻(Liagora orientalis)和波氏粉枝藻(Liagora boergesnii)絲狀體，其丙酮萃出物經過速分管柱層析得到一系列雙糖基二醯基甘油，經由核磁共振譜、紅外光吸收光譜、電子撞擊質譜分析及快速原子撞擊質譜及利用化學反應後進行甲基酯及糖衍生物的氣相層析質譜，推測此一系列化合物結構如下： 該一系列化合物的結構圖（略） 1：R,R′= C16:0, C16:0 2: C18:1, C16:0 3: C20:5, C16:0 4: C20:4, C16:0 其中雙糖基反應成糖的TMS衍生物及alditol acetate衍生物和部份甲基化的alditol acetate進行氣相層析質譜分離分析，證實為1,6鍵結之雙半乳糖；而醯基甘油上的脂肪酸在東方粉枝藻為 C16:0 (58.57%), C18:1 (16.48%), C20:5 (11.44%), C20:4 (3.71%)；而波氏粉枝藻則為 C16:0 (45.48%), C18:l (12.34%), C20:5 (32.21%), C20:4 (1.51%）的組成存在。 雙糖基二醯基甘油的溶血活性及抗血小板凝集活性並不存在。 第二部份 圈扇藻（Zonaria diesingiana）醯基間苯三酚類化合物的分離與光譜分析 本省北海岸採集的圈扇藻（Zonaria diesingiana）分離出兩個醯基間苯三酚類化合物，其中之一為 2-（17’-hydroxy-l’-oxoeicosa-5',8',11',14'(all Z)-tetraenyl）-l,3,5-trihydroxybenzene，首次以二維核磁共振譜對各碳13原子核化學位移重新鑑別，並提出舊有文獻（Gerwick & Fenical, 1982）對 C-3', C-4', C-16', C-18’數據上的修正；另一為 2-（ 1’-oxoeicosa-5',8',11',14',17' (all Z)－pentaenyl)-1,3,5-trihydroxybenzene，同樣亦對舊有文獻（Amicoet.al.,1981）上 C-3',C-4’之化學位移提出修正。並提出分子內氫鍵形成之有無在核磁共振譜上的表現情形。 | zh_TW |
| dc.description.abstract | Part Ⅰ: Isolation and characterization of digalactosyldiacylglycerols from Liagora orientalis and L. boergesnii. A series of digalactosyldiacylglycerols has been isolated from cultured filamentous phase of L. orientalis and L. boergesnii (Rhodophyta). These were extracted with acetone and seperated through Si-flash column chromotography, and their structures were characterized by EIMS, FABMS and NMR, IR spectroscopic methods. The galactose residues of digalactosyldiacylglycerols have been determined as 1,6-linked digalactose through GCMS analysis of their TMS-sugar derivatives, alditol acetates and partially methylated alditol acetates. The fatty acid residues of digalactosyldiacylglycerols from L. orientalis have been determined as C16:0 (58.57%), C18:l (16.48%), C20:5 (11.44%), C20:4 (3.71%), and the fatty acid residues of digalactosyldiacylglycerols from L. boergesnii have been determined as C16:0 (58.57%), C18:l (16.48%), C20:5 (11.44%), C20:4 (3.71%). The structure is sbbreviated. 1: R, R′= C16:0, C16:0 2: C18:1, C16:0 3: C20:5, C16:0 4: C20:4, C16:0 These compounds were then subjected to hemolysis and antiplatelet acativty assay, there is no significant difference compared with the control. Part Ⅱ: Isolation and characterization of acylphlorglucinols from Zonaria diesingiana. Two acyiphiorglucinols have been isolated from Zonaria diesingiana collected from the north coast in Taiwan. Based on 1H, 13C, 1H-1H COSY and 13C-1H COSY NMR, one is assigned as 2-(17’-hydroxy-l’-oxoeicosa-5’, 8’, 11’, 14’ (all Z)-tetraenyl)-1,3,5-trihydroxybenzene, and its 13C-NMR data at C-3’, C-4’, C-16’, C-18’ are different from the published report (Gerwick & Fenical,1982).The other is 2-(1’-oxoeicosa-5’,8’,11’,14’,17’(all Z)-pentaenyl)-l ,3,5-trihydroxybenzene, and its 13C-NMR data at C-3’and C-4’are also different from the published report (Gerwick & Fenical,1982).This is the first time determing these two structures by two dimention NMR, and make some corrections on the published data. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:20:21Z (GMT). No. of bitstreams: 0 Previous issue date: 1997 | en |
| dc.description.tableofcontents | 第一部份 東方粉枝藻(Liagora orientalis)及波氏粉枝藻(Liagora.boergensnii)雙半乳糖基二醯基甘 油的分離與結構鑑定 壹、前言………………………………………………………………………………………………1 貳、材料與方法……………………………………………………………………………………………7 一、藥品與重要儀器…………………………………………………………………………………7 二、藻體培養…………………………………………………………………………………………7 三、化合物的萃取與分離……………………………………………………………………………9 四、混合物(A)、(B)結構 鑒定………………………………………………………………………9 1、磁共振譜分析…………………………………………………………………………………9 2、紅外光吸收光譜分析…………………………………………………………………………10 3、電子撞擊質譜分析……………………………………………………………………………10 4、混合物醣基部份分析…………………………………………………………………………10 A、糖TMS衍生物分析…………………………………………………………………………10 B、糖alditol acetate衍生物分析…………………………………………………………11 C、鍵結點分析 ………………………………………………………………………………12 5、混合物脂防酸部份分析………………………………………………………………………13 6、快速原子撞擊質譜分析………………………………………………………………………13 五、生物活性測試……………………………………………………………………………………14 1、溶血活性測試……………………………………………………………………………………16 A、標準品Digitonin濃度對溶血百分比檢量線製作………………………………………14 B、混合物(A)、(B)溶血活性之測定 ………………………………………………………15 2、抗血小板凝集測試……………………………………………………………………………16 A、血小板懸浮液的準備 ……………………………………………………………………16 B、抗血小板凝集測定 ………………………………………………………………………16 參、結果與討論……………………………………………………………………………………………18 一、 混合物(A)、(B)結構鑑定 ……………………………………………………………………18 混合物(A) ……………………………………………………………………………………………18 1、雙糖基二醯基甘油的確認 ………………………………………………………………………18 A、核磁共振譜分析 ………………………………………………………………………………18 B、紅外光吸收光譜分析 …………………………………………………………………………18 C、電子撞擊質譜分析 ……………………………………………………………………………20 2、醣基部份分析 ……………………………………………………………………………………20 A、糖TMS衍生物分析………………………………………………………………………………20 B、糖alditol acetate衍生物分析………………………………………………………………22 C、鍵結點分析 ……………………………………………………………………………………24 3、脂肪酸部份分析 …………………………………………………………………………………24 4、快速原子撞擊質譜分析 …………………………………………………………………………27 混合物(B) ……………………………………………………………………………………………27 1、醣基部份分析 ……………………………………………………………………………………27 A、糖TMS衍生物分析………………………………………………………………………………27 B、糖alditol acetate衍生物分析………………………………………………………………………………27 C、鍵結點分析………………………………………………………………………………………………28 2、脂肪酸部份分析………………………………………………………………………………………………28 3、快速原子撞擊質譜………………………………………………………………………………………………29 二、生物活性測試………………………………………………………………………………………………30 1、溶血活性測試………………………………………………………………………………………………30 A、標準品Digitonin濃度對溶血百分比檢量線製作………………………………………………………………… B、混合物(A)、(B)溶血活性之測定…………………………………………………………………………………31 2、抗血小板凝集測試………………………………………………………………………………………………32 第二部份 圈扇藻（Zonaria diesingiana）醯基間苯三酚纇化合物的分離與光譜分析 壹、前言………………………………………………………………………………………………34 貳、材料與方法………………………………………………………………………………………………38 一、藥品與重要儀器………………………………………………………………………………………………38 二、材料………………………………………………………………………………………………38 三、藻體萃取與純化………………………………………………………………………………………………39 四、化合物結構………………………………………………………………………………………………39 1、核磁共振譜分析………………………………………………………………………………………………39 2、紫外光吸收光譜分析……………………………………………………………………………………………40 3、電子撞擊質譜分析………………………………………………………………………………………………40 參、結果與討論………………………………………………………………………………………………41 一、化合物(11)、(12)結構鑑定………………………………………………………………………………………41 1、化合物(11)………………………………………………………………………………………………41 2、化合物(12)………………………………………………………………………………………………46 肆、參考文獻………………………………………………………………………………………………51 附表………………………………………………………………………………………………57 附圖………………………………………………………………………………………………61 | |
| dc.language.iso | zh-TW | |
| dc.title | 東方粉枝藻及波氏粉枝藻雙半乳糖基二醯基甘油及圈扇藻醯基間苯三酚類化合物的分離與其結構鑑定 | zh_TW |
| dc.title | Isolation and characterization of digalactosyl-diacylg1ycerols from Liagora orientalis and L. boergensnii and acylphlorglucinols from Zonaria diesingiana | en |
| dc.date.schoolyear | 85-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 101 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
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