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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76314完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 陳亮明 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:20:11Z | - |
| dc.date.available | 2021-07-01T08:20:11Z | - |
| dc.date.issued | 1997 | |
| dc.identifier.citation | (1) DANIEL J. LEAHY (1995) A structural view of CD4 and CD8 . FASEB J. 9, 17-25 (1995) (2) Littman, D, R., Thomas, Y., Maddon , P. J., Chess, L., and Axel, R. (1985)The isolation and sequence of the gene encoding T8 : a molecule defining functional classes of T lymphocytes. Cell 40, 237- 246 (3) Jack L. Strominger (1989). Developmental biology of Tcell receptors Science, 244, 943-244 (4) Gerald R. Crabtree (1989). Contingent genetic regulatory events in T lymphocyte activation. Science, 243, 355-361 (5) Harald Von Boehmer (1994). Positive selection of lymphocytes. Cell, 76, 219-288 (6) Von Boehmer, H. (1986). The selection of the α , β heterodimeric Tcell receptor for antigen. Immunol. Today, 7, 333-336 (7) Robey, E. A., Fowlkes, B. J., Gordon, J. W., Kioussis, D., Von Boehmer, H., Ramsdell, F., and Axel, R. (1991). Thymic selection in CD8 transgenic mice supports an instructive model for commitment to a CD4 or CD8 lineage. Cell, 64, 99-107 (8) Dan R.Littman (1987). The structure of the CD4 and CD8 genes. Ann.Rev. Immunol., 5, 561-584 (9) MacDonald, H. R., Glasebrook, A. L., Bron, C., Kelso, A., Cerotti, J. , -C. (1982). Clonal heterogeneity in the functional requirement for Ly 2, 3 molecules on cytolytic Tlymphocytes(CTL). Immunol. Rev., 68, 88-155 (10) Arthur Weiss, Dan R. Littman (1994). Signal transduction by lymphocyte antigen receptors. Cell, 76, 263-274 (11) Julia M. Turner, Michael H. Brodsky, Bryan A. Irving, Steven D.Levin, Roger M. Permutter, and Dan R. littman (1990). Interaction of the unique N-terminal region of tyrosine kinase p56Lck with cytoplasmic domains of CD4 and CD8 is mediated by cysteine motifs. Cell, 60, 755-765 (12) Tomas mustelin and Paul Burn (1993). Regulation of src family tyrosine kinase in lymphocytes. T. I. B. S, 18, 215-220 (13) Johnson, P., Wi1liams, A. F. (1986). Striking similarities between antigen receptor J pieces and sequence in the second chain of the murine CD8 antigen. Nature, 323, 74-76 (14) Hiromitsu Nakauchi, Massatoshi Tagawa, Garry P. Nolan, and Leonard A.Herzenberg (1987). Isolation and characterization of the gene for the Murine T cell Differentiation antigen and immunoglobulin-related molecule, Lyt-2 Nucleic Acid Res., 15, 4337-4347 (15) Anne M. Norment, Nils lonberg, Elizabeth Lacy, and Dan R. Littman(l989). Alternatively spliced mRNA encodes a secreted form of Human CD8 α. J. Immunol, 142, 3312-3319 (16) Kei-Ichi Nakayama, Yo-Ichi Shinkai, KO Okumura, and Hiromitsu Nakauchi (1989). Isolation and characterization of the mouse CD8βchain(ly-3) genes. J. Immunol, 142, 2540-2546 (17) David M. Gibson, Benjammin A. Taylor , and Marianna Cherry(l978). Enidence for close linkage of a mouse light chain marker with the Ly -2, 3 locus. J, Immunol, 121, 1585-1590 (18) Scott D. Gorman, Y.henry Sun , Rose Zamoyska, and Jane R.Parnes(1988). Molecular linkage of the Ly-3 and Ly-2 Locus. J, Immunol, 121, 1585-1590 (19）廖泓鈞(1994) ，雞的 CD 8 α基因的分離與鑑定． 國立台灣大學碩士論文 (1994) (20) F. Sanger, S. L. Nicklen and A.R. Coulson (1977). DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. Usa, 74, 5463-5467 (21) M. A. Innis, K. B. Myambo, D. H. Gelfand and M. A. D. Brow (l988).DNA sequencing with Taq DNA polymerase and direct sequencing of polymerase chain reaction - amplified DNA. Proc. Natl. Aad. Sci. USA, 85, 9436-9440 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76314 | - |
| dc.description.abstract | CD8分子是一種由T細胞表現至細胞表面的醣蛋白，由α和β兩條勝?鏈以Heterodimer結構存在。其所扮演的角色為與抗原呈現細胞（Antigen Prensting Cell）中的MHC Classl I 分子結合，及參予Cytotoxic T Cell本身的活化。 本實驗主要目的為定出CD8 α基因在雞的genome上的結構以幫助瞭解此基因在演化上可能形成的過程，並希望藉此能找到與CD8 α基因表現上有關的調控位元或機轉。目前我們的研究結果得到一與演化有關的結果。我們由目前定出的CD8 α基因，CD8 α上游約15 kb的序列，進入GCG搜尋，發現不少基因片段和人跟老鼠的Ig kLight Chain極為相似。同時在我們定出15 kb的序列中，6046?6712和部分CD8 α structure gene與Ig k Light Chain 有極高相似性。CD8 α與Ig k Light Chain皆為Immunoglobulin Superfamily的一員。在這段序列中我們找到 Immunoglobulin形成Floding時所必須的Cysteine，這意味著這段基因有某個Conserve的特殊結構為 Immunoglobulin所使用。 雞至今仍未發現有Ig k Light Chain的存在，在人跟老鼠Ig k Light Chain和CD8分子基因位於一條染色體上，如果雞有Ig k LightChain ，這些和Ig k LightChain相似的基因片段可能就是Ig k Light Chain的一部份，或其前身。人類和老鼠則為增加免疫球蛋白的變異性，而演化出Ig k Light Chain來。雞則可能因為屬於較低等的動物、對免疫球蛋白變異性需求不這麼高，所以沒有Ig k Light Chain演化出。 在這段約15kb的序列中我們還找到兩段Reverse Repeat的DNA片段，我們將這兩段基因送入GCG搜尋發現其中有一段序列Immunoglobulin Fc Receptor的基因很像。至於這兩段DNA片段所代表的意義還不是很清楚，有待進一步的研究。 | zh_TW |
| dc.description.abstract | CD8 molecules are cell-surface glycoproteins that participate in both T cell development and antigen recognition by T cells . CD8 exists as a disulfide-linked heterodimer of the α and β chains . The sequence encoding chicken genomic CD8 α gene was proviously determined in this laboratory . In order to futher understand the mechanism involved in regulating the expression of CD8 αgene , we extended our studies by determining the flanking sequences surrounding the CD8 α coding region. We found some DNA fragments upstream of the chicken CD8 α gene displaying squence similarity to the coding region of CD8 α gene and Ig k light chain genes of human and mouse. A block of DNA in the 15 kb region was also found to be a partial duplicate of the CD8 α gene . Till now no Ig k light chain is documented to be expressed in the chicken . In human and mouse , CD8 α gene and Ig k light chain are located in the same chromosome . If chickens do have Ig k light chain ,these DNA fragment with sequences similar to the Ig K light chain might be part of the pseudogenes of the chicken Ig k V gene members. More experiments are needed to determine whether there is (pseudo-) Ig k loci present in chickens . Two different inverted repeats were found in the 15 kb region . One of them showed certain degree of sequence similarity to Ig Fc receptor . Again , more experiments are needed to help us understanding the meanings of these DNA fragments . | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:20:11Z (GMT). No. of bitstreams: 0 Previous issue date: 1997 | en |
| dc.description.tableofcontents | 中文摘要 1 英文摘要 2 縮寫表 3 緒言 4 實驗方法和材料 11 結果 13 討論 15 圖表 18 附錄 38 參考文獻 39 | |
| dc.language.iso | zh-TW | |
| dc.title | 雞的CD 8α基因Flanking Region結構之研究 | zh_TW |
| dc.title | Genomic Structural Studies of Chicken CD 8 αGene Flanking Region | en |
| dc.date.schoolyear | 85-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 45 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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