請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76181完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.author | 葛茂豐 | zh_TW |
| dc.date.accessioned | 2021-07-01T08:18:51Z | - |
| dc.date.available | 2021-07-01T08:18:51Z | - |
| dc.date.issued | 1995 | |
| dc.identifier.citation | 郭書祥。（1984）以抗藥性標誌進行酵母菌載體之改良。國立臺灣大學植物科學研究所碩士論文。 Brechot, C. (1994) Primary liver cancer. Chapter 3 pp.31-40, Chapter 8 pp. 89-124 CRC Press, Inc. Boca Raton Chen, D. C. (1994) Studies on the expression of HBsAg in secretion pathway of Saccharomyces cerevisia. Ph. D. Thesis, Institute of Botany, National Taiwan University. Chen, D. C. Yang. B. C. and Kuo. T. T. (1991) One-step transformation of yeast in stationary phase. Current Genetics 85: 84-84 Chen. D. C. Chuang, L. T. Chen. W. P. and Kuo, T. T. 1995) Abnomal growth induced by expression of HBsAg in the secretion pathway of S. cerevisiae pep4 mutants. Current Genetics 27: 201-206 Chisari, F. V. Klopchin, K. Moriyama, K. Pasquinelli, C. Dunsford, H. A. Sel1 , S. Pinkert, C. A. Brinster, R. L. and Palmiter R. D. (1989) Molecular pathogenesis of hepatocellular carcinoma in hepatitis B virus transgenic mice. Cell 59: 1145-1156 Cross, F. R. and Tinkelenberg, A. II. (1991) A potential positive feedback loop controlling CLN1 and CLN2 gene expression at start of the yeast cycle. Cell 65: 875-883 Fantes, P. and Brooks, R. (1994) The cell cycle----a practical approach. Chapter 2 pp.25-44, Chapter 4 pp.69-92 Oxford University Press, Inc. Oxford Fourel, G. Couturier, J. Wei, Y. Apiou, F. Tiollais, P. and Buendia, M. A. (1994) Evidence for long-rang oncogene activation by hepadnavirus insertion. The EMBO Journal 13: 2526-2531 Ganem, D. and Varmus H. E. (1987) The molecular biology of the hepatitis B virus. Annual Review of Biochemistry 56: 651-694 Gimeno, C. J. Ljungdahl, P. O. Styles, C. A. and Fink, G. R. (1992) Unipolar cell divisions in the yeast S. cerevisiae lead to filamentous growth: regulation by starvation and RAS. Cell 68: 1077-1090 Gimeno, C. J. and Fink, G. R. (1994) Induction of pseudohyphal growth by overexpression of PHDI, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development. Molecular and Cellular Biology 14: 2100-2112 Helling, R. B. Vargas, C. N. and Adams, J. (1987) Evolution of Escherichia coil during growth in a constant Environment. Genetics 116: 349-358 Hsu, C. Tokiwa, T. Bennett, W. Metcalf, R. A. Welsh, A. Sun, T. and Harris, C. C. (1993) 53 gene mutation and integrated hepatitis B viral DNA sequences human liver cancer cell lines. Carcinogenesis 11: 987-992 Jones, E. W. (1977) Proteinases mutants of Saccharomyces cerevisiae. Genetics 85: 23-33. Jones, E. W. (1991) Three proteolvtic systems in the yeast Saccharomyces cerevisiae. The Journal of Biological Chemistry 26: 7963-7966 Kekule, A. S. Lauer, U. Meyer, M. Caselmann,W. H. Hofschneider, P. H. and Korshy. R. (1990) The preS2/S region of integrated hepatitis B virus DNA encodes a transcriptional transactivator. Nature 343: 457-461 Kim, C. M. Koike, K. Saito. I. Miyamura, T. and Jay, G. (1991) Hbx gene of hepatitis B virus induces liver cancer in transgenic mice. Nature 351: 317-320 Koch, C. and Nasmyth, K. (1994) Cell cycle regulated transcription in yeast. Current Opinion in Cell Biology 6:451-459 Kron, S. J. Styles, C. A. and Fink, G. R. (1994) Symmetric cell division in pseudohyphal of the yeast Saccharomyces cerevisiae. Molecular Biology of the Cell 5: 1003-1022 Lew, D. J. and Reed, S. I. (1993) Morphogenesis in the yeast cell cycle: regulation by CDC28 and cyclins. The Journal of Cell Biology 120: .1305-1320 Mabit, H. Dubanchet, S. Capel, F. Dauguet, C. and Petit, M. A. (1994) In vitro infection of human hepatoma cells (HepG2) with hepatitis B virus (HBV): spontaneous selection of a stable IIBV surface antigen-producing HepG2 cell line containing integrated HBV DNA sequences. Journal of General Virology 75: 2681-2689 Moreno, S. Hayles, J. and Nurse, P. (1989) Regulation of p34cdc2 protein during mitosis. Cell 58: 361-372 Moutain, H. A. and Sudberv, P. E. (1990) The relationship of growth rate and catabolite repression with WHI2 expression and cell size in Saccharomyces cerevisiae. Journal of General Microbiology 136: 733-737 Nasmyth, K. (1993) Control of yeast cell cycle by the CDC28 protein kinase. Current Opinion in Cell Biology 5: 166-179 Roberts, R. L. and Fink, G. R. (1994) Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth. Genes & Development 8: 2974-2985 Sprague, G. F. (1992) Strategies for sexual differentiation in budding yeast, fission yeast, and corn smut fungus. Seminars in Developmental Biology 3: 299-306 Stueland, C. S. Lew, D. J. Cismowski, M. J. and Reed, S. I. (1993) Full activation of p34cdc28 histone ill kinase activity is unable to promote entry into mitosis in checkpoint-arrest cells of the yeast Saccharomyces cerevisiae. Molecular and Cellular Biology 13: 3744-3755 Surana. U. Robitsch, H. Price, C. Schuster, T. Fitch, I. Futcher, A. B. and Nasmyth, K. (1991) The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae. Cell 65: 145-161 Takada. S. and Koike, K. (1990) Trans activation function of a 3’ truncated X gene-cell fusion product from integrated hepatitis B virus DNA in chronic hepatitis tissues. Proceedings of the National Academy of Sciences 87: 5628-5632 Teichert, V. Mechler, B. Mu11ar. H. and Wolf, D. H. (1989) Lysosomal (vacuolar) proteinase of yeast are essential catalysys for protein degradation differentiation and cell survival. The Journal of Biological Chemistry 264: 16037-16045 Tokiwa. G.Tyers. M. Volpe, T. and Futcher, B. (1994) Inhibition of G1 cyclin activity by the Ras/cAMP pathway in yeast. Nature 371: 342-345 Tsurimoto. T Gripon. P Fourel, I Hantz, O. Trepo, C. and Guguen-Guillouzo, C. (1987) Stable expression and replication of hepatitis B virus genome in an integrated state in a human hepatoma cell line transfected with the cloned viral DNA. Proceedings of the National Academy of Sciences 84:444-448 Vyas. G. N. Dienstag. J. L. and Hoofnagle, J. H. (1994) Viral hepatitis and Liver Disease. Chapter 4-6 pp.49-96 Grune & Stratton, Inc. Orlando Wang, J. Chenivesse, X. Henglein, B. and Brechot, C. (1990) Hepatitis B virus integration in a cyclin A gene in a hepatocellular carcinoma. Nature 343: 555-557 Wang, J. Zindy, F. Chenivesse, X. Lamas, E. Henglein, B. and Brechot, C. (1992) Modification of cyclin A expression by hepatocellular carcinoma. Oncogene 7: 1653-1656 Woodcook. D. M. Growther, P. J. Doherty, J. Jefferson. S. DeCurz. E. Widner. M. Smith. S. S. Michael, M. Z. and Graham, M. W. (1989) Quantitative evalution of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acid Research 17: 3469-3478 oo1ford. C. A. Noble. J. A. Garmant, J. D. Tarn, M. F. and Jonest, E. W. (1993) Phenotypic analysis of Proteinase A mutant. The Journal of Biological Chemistry. 268: 8990-8998 Zhang, Y. J. Jiang, W. Chen, C. J. Lee, C. S. Kahn, S. M. Santella, R. M. and Weinstein, I. B. (1993) Amplification and overexpression of cyclin Dl in human hepatocellular carcinoma. Biochemical and Biophysical Research Communications 196: 1010-1016 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76181 | - |
| dc.description.abstract | B型肝炎病毒HBV(hepatitis B virus)在肝癌(hepatocellular carcinoma)發生上所扮演的角色以及對真核生物的影響仍不清楚。近年發現病毒的蛋白質會改變宿主基因的表現，進而導致肝癌的發生。過去本實驗室的研究發現，將一個帶有B型肝炎表面抗原(HBsAg)基因的質體送到酵母菌中，表現後使其進入酵母菌的分泌途徑(secretion pathway)中，會使得酵母菌的生長受到嚴重的抑制。本實驗篩選一株生長特別快速而且不受B型肺炎病毒表面抗原蛋白質毒性抑制的穩定轉形株NI(non-inhibited)。結果顯示NI具有較高的生長速率，飽和期(stationary phase)有較高的細胞存活率(cell viability)，缺糖飢餓(glucose starvation)時有較高的histone H1 kinase activity，較高的plasmid TRP1 marker Stability，較高的表面抗原(HBsAg)產量，偽絲狀生長(pseudohyphae growth)，及integration現象，使酵母菌變成類似癌細胞之生長模式。由於在肝癌的研究上一直缺乏良好的研究系統，無法進行深入的研究，酵母菌可以提供作為研究B型肝炎病毒(HBV)作用的研究系統。 | zh_TW |
| dc.description.abstract | The exact role of hepatitis B virus in the development of liver cancer is not clear. The recent identification of some viral regulatory genes suggest a possible direct involvement of the viral protein may alter host gene expression and lead to the development of hepatocellular carcinoma. According to previously study, yeast cells were transformed with a plasmid containing DNA fragment express the HBsAg gene in secretion pathway of yeast S. cerevisiae and it has been shown that the growth of these transformants are strongly inhibited. This study select a new population which are non-inhited (NI). These NI transformants were characterized in higher growth rate, higher cell viability, higher plasmid TRP1 marker stability, higher histone Hl kinase activity, pseudohyphae growth, HBV DNA integration. Because there is no good research system in liver cancer study, these results presented here suggest that these transformants is similar with cancer cells in cell growth and the S. cerevisiae may provide a simple system for studying the mechanism of action of HBV virus. | en |
| dc.description.provenance | Made available in DSpace on 2021-07-01T08:18:51Z (GMT). No. of bitstreams: 0 Previous issue date: 1995 | en |
| dc.description.tableofcontents | 壹、緒言. . . . . . . . . . . . . . . . . . . 1 貳、材料與方法 （一）材料. . . . . . . . . . . . . . . . . . .4 （二）方法. . . . . . . . . . . . . . . . . . .10 參、結果. . . . . . . . . . . . . . . . . . . 18 肆、討論. . . . . . . . . . . . . . . . . . .24 伍、參考文獻. . . . . . . . . . . . . . . . . . .56 | |
| dc.language.iso | zh-TW | |
| dc.title | B型肝炎病毒表面抗原在酵母菌分泌途徑表現所?生的生物效應 | zh_TW |
| dc.title | Biological effects of HBsAg expression in secretion pathway of yeast Saccharomyces cerevisiae | en |
| dc.date.schoolyear | 83-2 | |
| dc.description.degree | 碩士 | |
| dc.relation.page | 69 | |
| dc.rights.note | 未授權 | |
| dc.contributor.author-dept | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
文件中的檔案：
沒有與此文件相關的檔案。
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。