請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76179
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Kai-Tai Chang | en |
dc.contributor.author | 張開泰 | zh_TW |
dc.date.accessioned | 2021-07-01T08:18:50Z | - |
dc.date.available | 2021-07-01T08:18:50Z | - |
dc.date.issued | 1978 | |
dc.identifier.citation | 1. Allington, R.W., M.K. Brakks, J.M. Nelson, C.G.Aron, and B.A. Larkins. 1976. Optimum conditions for high resolution gradient analysis. Anal. Biochem. 73:78-92. 2. Bayley, J.M., J. King, and O.L. Gamborg. 1972. The effect of the source of inorganic nitrogen on growth and enzymes of nitrogen assimilation in soybean and wheat cells in suspension culfures. Planta (Berl.) 105:15-24. 3. Bayley, J.M., J. King, and O.L. Gamborg. 1972. The ability of amino compounds and conditioned medium to alleviate the reduced nitrogen requirement of soybean cells grown in suspension cultures. Planta (Berl.) 105:25-32. 4. Behrend, J., and R.I. Mateles. 1975. Nitrogen metabolism in plant cell suspension cultures. I. Effect of amino acid on growth. Plant Physiol. 56:584-589. 5. Birmingham, B.C., and G.A. Maolachlan, 1972. Generation and suppression of microsomal ribonuclease activity after treatments with auxin and cytokinin. Plant Physiol. 49:371-375. 6. Cashel, M.1975. Regulation of bacterial ppGpp. and pppGpp. Ann. Rev. Microbiol. 29:301-318. 7. Chambon, P. 1975. Eukaryotic nuclear RNA polymerases. Ann. Rev. Biochem. 44:613-638. 8. Chen, Y.M., C.Y. Lin, H.Chang, T.J. Guilfoyle, and J.L. Key. 1975. Isolation and properties of nuclei from control and auxin-treated soybean hypocotyl. Plant Physiol. 56:78-82. 9. Davies, E., B.A.Larkins, and R.H.Knight. 1972. Polyribosomes from peas. I An improved method for their isolation in the absence of ribonucleases inhibitors. Plant Physiol. 50:581-584. 10. Duda, C.T. 1976. Plant RNA polymerases. Ann. Rev. Plant Physiol. 27:119-132. 11. Fosket, D.E., and K.C. Short. 1973. The role of cytokinin in the regulation of growth, DNA synthesis and cell proliferation in cultured soybean tissues, Glycine max L. var. Biloxi. Physiol. Plant, 28:14-23. 12. Fosket, D.E., M.J.Volk, and M.R. Goldsmith. 1977 Polyribosome formation in relation to cytokinin-induced cell division in suspension cultures of Glycine max L. Merr. Plant Physiol. 60:554-562. 13. Gamborg. O.L., R.A.Miller, and K. Ojima. 1968 Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151-158. 14. Gamborg, O.L., and L.R. Wetter, 1975. Plant tissue culture methods, National Research Council of Canada, Prairie Regional Laboratory, Saskatoon Saskatchewan. 15. Gamborg, O.L., T.Murashige, T.A. Thorpe, and I. K.Vasil. 1976. Plant tissue culture media. In Vitro. 12:473-478. 16. Gross, K.J. and A.O.Pogo. 1974. Control mechanism of ribonucleic acid synthesis in eukaryotes. The effect of amino acid and glucose starvation and cycloheximide on yeast deoxyribonucleic acid-dependent ribonucleic acid polymerases. J. Biol. Chem. 249:568-576. 17. Gross, K.J., and A.O. Pogo. 1976. Control of ribonucleic acid synthesis in eukaryotes. 2. The effect of protein synthesis on the activities of nuclear and total DNA-dependent RNA polymerase in yeast. Biochemistry. 15:2070-2081. 18. Guilfoyle, T.J., C.Y.Lin, Y.M. Chen, R.T.Nagao, and J.L. Key. 1975. Enhancement of soybean RNA polymerase I by auxin. Proc. Nat. Acad. Sci. U.S.A. 72:69-72. 19. Hallberg, R.L., and P.J. Bruns. 1976. Ribosome biosynthesis in Tetrahymena pyriformis, Regulation in response to nutritional changes. J. Cell Biol. 71:383-394. 20. Ingle, J., and J.L. Key, 1965. A comparative evaluation of the synthesis of DNA-like RNA in excised and intact plant tissues. Plant Physiol. 40:1212-1219. 21. Key.J.L. 1969. Hormones and nucleic acid metabolism. Ann. Rev. Plant Physiol. 20;449-474. 22. Kumar, B.V.,R. McMillian, G. Medoff, D. Schlessinger, and G.S. Kobayashi. 1977. Mechanism of the inhibition by RNA of the RNA polymerases of Histoplasma capsulatum.Biochim. Biophys. Acta. 478:192-200. 23. Lin, C.Y., Y.M. Chen, T.J. Guilfoyle, and J.L. Key. 1976. Selective modulation of RNA polymerase I activity during growth transitions in the soybean seedling Plant Physiol. 58:614-617. 24. Lindell, T.J., F.Weinberg, P.W. Morris, R.G. Roeder, and W.J. Rutter. 1970. Specific inhibition of nuclear RNA polymerase II by α-amanitin. Science. 170:447-449. 25. McMillian, R., B.V. Kumar, G.Medoff, D.Schlessinger and G.S.Kobayashi. 1977. Inhibition of Histoplasma capsulatum ribonucleic acid polymerases by homologous and heterologous ribonucleic acid. J. Bacteriol, 130:1387-1389. 26. Murashige, T. 1974. Plant propagation through tissue cultures. Ann. Rev. Plant Physiol. 25:135-166. 27. Sasaki, R., H. Goto, K.Arima, and Y.Sasaki. 1974. Effect of polyribonucleotides on eukaryotic DNA-dependent RNA polymerases, Biochim. Biophys. Acta. 366:435-442. 28. Shulman, R.W., C.E. Sripati, and J.R. Warner. 1977. Noncoordinated transcription in the absence of protein synthesis in yeast. J. Biol. Chem. 252:1344-1349. 29. Simpkins, I., and H.E. Street. 1970. Studies on the growth in culture of plant cells, VII. Effects of kinetin on the carbohydrate and nitrogen metabolism of Acer pseudoplatanus, L. cells grown in suspension culture. J. Exp. Bot. 21:170-185. 30. Skoog, F., and D.J. Armstrong. 1970, Cytokinin. Ann. Rev. Plant Physiol. 21:359-384. 31. Street, H.E. 1973. Plant tissue and cell culture. Botanical monographs. Vol II. Blackwell scientific publication, London. 32. Travis, R.L., J.M. Anderson, and J.L.Key. 1973. Influence of auxin and incubation on the relative level of Polyribosomes in excised soybean hypocotyl. Plant Physiol. 52:608-612, 33. White, J. L., and H.H. Murakishi, 1977. Requirements for extraction of Polyribosomes from plant callus cultures. Plant Physiol. 59:800-802. 34. White, P.R. 1963. The cultivation of animal and plant cells 2nd ed. Ronald press. N.Y. 35.竹內正幸、石原愛也、古穀力、1972.基礎篇.植物組織培養.朝倉書店.東京.76-77。 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76179 | - |
dc.description.abstract | 大豆下胚軸頂端切離部份,在磷酸緩衝液(phosphate buffer)培養8至12小時,頂端部份核醣核酸聚合?I(RNA polymeraseI)的活性與蛋白質合成能力(polyribosome level)顯著的降低。 無機鹽(inorganic salt)、生長調節劑(growth regulator)、胺基酸源(amino acid source)對核醣核酸聚合?I活性的維持均有重大的影響。而含酵母焠取物(yeast extract)的B5配方培養液維持細胞活性,相對提高核醣核酸聚合?I活性與蛋白質合成能力,比其他的含酵母焠取物、椰子汁(coconut milk)、或乳酪素水解物(Casein hydrolysate)的MS. White. ER配方培養液,及含椰子汁或乳酪素水解物的B5配方培養液有較好的效果。核醣核酸聚合?I活性與蛋白質合成能力間好像有很密切的正相關存在。推測這種現象的產生是與大腸菌(E. coli)的stringent菌株(strain)胺基酸匱乏(amino acid starvation)所造成的stringency,有相似控制核醣體核醣核酸(ribosomal-RNA r-RNA)合成的機制。 | zh_TW |
dc.description.abstract | When exeised hypocotyl segments (0-0.5 cm) of dark-grown seedlings were incubated in phosphate buffer medium for 12 hours, the RNA polymerase I activity in the isolated nuclei decreased markedly relative to the RNA polymerase II activity. The level of polyribosome (protein synthetic activity) also decreased during the incubation period. It appears that there is a correlation between the RNA polymerase I activity and the level of polyribosome. Inorganic salts (macro-and micro-nutrients) such as MS or White, or B5 medium with plant growth hormones, which are commonly used in soybean tissue culture, in combination with either casein hydrolysate, yeast extract or coconut milk were used during the inoubation period to find whether the dissociation of the polyribosome could be prevented and the original RNA polymerase I activity be maintained. The B5 medium with yeast extract was found to be the most effective of the nutrients tested to maintain the RNA polymerase I activity up to 51% of the original activity. The level of polyribosome with this incubation medium was 47% which was 79% of the original polyribosome level. This substantiation shows the relationship between the level of polyribosome and the RNA polymerase I activity. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:18:50Z (GMT). No. of bitstreams: 0 Previous issue date: 1978 | en |
dc.description.tableofcontents | 中文摘要………………………………………1 英文摘要………………………………………2 前 言………………………………………4 材料與方法………………………………………7 結 果………………………………………14 討 論………………………………………23 引用文獻………………………………………29 | |
dc.language.iso | zh-TW | |
dc.title | 大豆下胚軸核醣核酸聚合?活性與蛋白質合成能力相關性之研究 | zh_TW |
dc.title | Studies on the correlation between RNA polymerase activity and polyribosome level in soybean hypocotyl | en |
dc.date.schoolyear | 66-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 39 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
沒有與此文件相關的檔案。
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。